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The map label for this gene is miaB [H]

Identifier: 57651862

GI number: 57651862

Start: 1327724

End: 1329268

Strand: Direct

Name: miaB [H]

Synonym: SACOL1312

Alternate gene names: 57651862

Gene position: 1327724-1329268 (Clockwise)

Preceding gene: 57651861

Following gene: 57651863

Centisome position: 47.26

GC content: 32.88

Gene sequence:

>1545_bases
GTGAACGAAGAACAAAGAAAAGCAAGTTCTGTAGATGTTTTAGCTGAGAGAGATAAGAAAGCAGAAAAAGATTATAGTAA
ATATTTTGAACATGTTTATCAGCCGCCTAATTTAAAAGAAGCGAAAAAAAGAGGTAAACAAGAAGTTCGTTATAATAGAG
ATTTCCAAATTGATGAAAAATATCGCGGTATGGGGAACGAGCGTACATTTTTAATTAAAACATATGGATGTCAAATGAAT
GCACATGACACTGAGGTCATTGCTGGTATACTTGAAGCATTAGGCTATCAAGCAACGACTGATATTAACACTGCAGATGT
TATTTTAATTAATACATGTGCGATTAGAGAAAATGCCGAGAACAAAGTGTTTAGTGAAATAGGTAATTTGAAGCATTTGA
AAAAAGAACGACCTGATATTTTAATCGGTGTTTGTGGTTGTATGTCACAAGAAGAGTCAGTAGTGAATAAAATTTTAAAA
TCGTATCAAAATGTAGATATGATATTTGGTACACATAATATTCATCATTTACCAGAAATTTTAGAAGAAGCATACTTATC
TAAAGCAATGGTTGTTGAAGTATGGTCTAAAGAAGGAGACGTTATTGAAAATCTTCCAAAAGTCCGTGAAGGCAACATTA
AAGCATGGGTCAATATTATGTATGGTTGTGATAAGTTTTGTACATATTGTATTGTTCCATTTACAAGAGGTAAAGAACGA
AGCCGTAGACCTGAAGACATTATAGATGAAGTACGTGAACTTGCTCGTGAAGGTTACAAAGAAATAACGCTTTTAGGTCA
AAATGTAAATTCTTATGGTAAAGATTTACAGGATATAGAATATGACTTAGGAGATCTTTTACAAGCAATTTCTAAAATAG
CGATTCCAAGAGTTCGTTTCACAACAAGTCATCCTTGGGACTTTACAGATCACATGATTGATGTTATTTCAGAGGGTGGT
AATATCGTTCCGCATATCCACTTGCCAGTTCAATCTGGAAATAATGCAGTATTAAAAATAATGGGTAGAAAATATACACG
AGAAAGTTATTTGGATTTAGTAAAACGAATCAAAGATAGAATTCCTAATGTAGCATTAACTACAGATATTATTGTAGGGT
ATCCAAATGAATCAGAGGAACAATTTGAAGAAACTTTAACTCTGTATGATGAAGTTGGTTTTGAACATGCATATACGTAC
TTGTATTCACAACGTGATGGTACGCCTGCTGCTAAAATGAAAGATAATGTACCTTTAAATGTCAAAAAGGAACGATTGCA
ACGTTTGAATAAAAAAGTTGGTCATTATTCACAAATAGCTATGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTG
AAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAA
GAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGT
AGAGCCGGAAATGGTGATTCAATAA

Upstream 100 bases:

>100_bases
CTTGTTAGAGATGACGTATAACTTTAACTGTTTTTGCACTTTATAGTTAAATTTAATATAATTATTAAATGATACGGGCA
AATAGAAAGGATTTTGTAAA

Downstream 100 bases:

>100_bases
ATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTATCAAAACATA
TCAACAAATTGAAGCACAGA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKRGKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMN
AHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVNIMYGCDKFCTYCIVPFTRGKER
SRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGG
NIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEVGFEHAYTY
LYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPK
EMIGKLVEVRIDEAKQYSLNGSFIKEVEPEMVIQ

Sequences:

>Translated_514_residues
MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKRGKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMN
AHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVNIMYGCDKFCTYCIVPFTRGKER
SRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGG
NIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEVGFEHAYTY
LYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPK
EMIGKLVEVRIDEAKQYSLNGSFIKEVEPEMVIQ
>Mature_514_residues
MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKRGKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMN
AHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVNIMYGCDKFCTYCIVPFTRGKER
SRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGG
NIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEVGFEHAYTY
LYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPK
EMIGKLVEVRIDEAKQYSLNGSFIKEVEPEMVIQ

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=443, Percent_Identity=37.6975169300226, Blast_Score=288, Evalue=8e-78,
Organism=Homo sapiens, GI28872784, Length=443, Percent_Identity=37.6975169300226, Blast_Score=288, Evalue=1e-77,
Organism=Homo sapiens, GI93277076, Length=445, Percent_Identity=28.314606741573, Blast_Score=179, Evalue=6e-45,
Organism=Escherichia coli, GI1786882, Length=443, Percent_Identity=41.0835214446953, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1787057, Length=462, Percent_Identity=25.5411255411255, Blast_Score=152, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=473, Percent_Identity=33.1923890063425, Blast_Score=251, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI71996771, Length=364, Percent_Identity=27.1978021978022, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21356207, Length=482, Percent_Identity=37.7593360995851, Blast_Score=301, Evalue=5e-82,
Organism=Drosophila melanogaster, GI19922432, Length=459, Percent_Identity=30.2832244008715, Blast_Score=189, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 58932; Mature: 58932

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKRGKQEVRYNRDFQIDEK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHCCCCCCCCCHH
YRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAE
HCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCH
NKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHHLPEI
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHH
LEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVNIMYGCDKFCTYCIVPFTRGKER
HHHHHHHHHHHEEECCCCCCHHHHCCCCCCCCHHEEHHHHHHHHHHHHHEEEECCCCCCC
SRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKIAIPRVRF
CCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEE
TTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDR
ECCCCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHH
IPNVALTTDIIVGYPNESEEQFEETLTLYDEVGFEHAYTYLYSQRDGTPAAKMKDNVPLN
CCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHEECCCCCCHHHCCCCCCCC
VKKERLQRLNKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPK
CHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEECCCCCCCEEECCCCH
EMIGKLVEVRIDEAKQYSLNGSFIKEVEPEMVIQ
HHHHHHHHHHHCCCCEECCCCCHHHHCCCHHCCC
>Mature Secondary Structure
MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKRGKQEVRYNRDFQIDEK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHCCCCCCCCCHH
YRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAE
HCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCH
NKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHHLPEI
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHH
LEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVNIMYGCDKFCTYCIVPFTRGKER
HHHHHHHHHHHEEECCCCCCHHHHCCCCCCCCHHEEHHHHHHHHHHHHHEEEECCCCCCC
SRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKIAIPRVRF
CCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEE
TTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDR
ECCCCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHH
IPNVALTTDIIVGYPNESEEQFEETLTLYDEVGFEHAYTYLYSQRDGTPAAKMKDNVPLN
CCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHEECCCCCCHHHCCCCCCCC
VKKERLQRLNKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPK
CHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEECCCCCCCEEECCCCH
EMIGKLVEVRIDEAKQYSLNGSFIKEVEPEMVIQ
HHHHHHHHHHHCCCCEECCCCCHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA