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The map label for this gene is ymfI [H]

Identifier: 57651850

GI number: 57651850

Start: 1315795

End: 1316499

Strand: Direct

Name: ymfI [H]

Synonym: SACOL1299

Alternate gene names: 57651850

Gene position: 1315795-1316499 (Clockwise)

Preceding gene: 57651849

Following gene: 57651851

Centisome position: 46.84

GC content: 34.04

Gene sequence:

>705_bases
ATGAAAGCATTAGTATTAGGTGGTTCTGGTTCAATTGGTTCTGAGATAGTCAAACAATTATTAACTGATGGATTTGAAGT
TTATGTGCAATATTATCGTACTGATATAAATGAATTAACTAGCAAATTTAATGATGATAAAGTTCGTTTTATACAAGCGG
ATTTATCTCAAACAATTGATATTGACAAAACATTTGGTGACATTAAATCATTAGACTGTTTAATATATGCAAGTGGTCAG
TCTTTATATGGTGTTTTACAAGATATGAAAGACCATGATATTGATGCATGTTATCAGTTAAATGTCTTGCAATTAATTCG
ATTATGTAGATATTTCGTTGATGTTTTACGTCAAAGTGCCAATGGAAGAATTATTGTAATTTCATCAATTTGGGGTGAGA
CAGGAGCTAGTATGGAAACTATTTATTCGGCGATGAAAAGTGCACAATTAGGTTTCGTTAAGGCGCTTAGTCAAGAGCTT
GCACTAACATCAGTGACAGTAAATGCTATCGCACCTGGATTTGTAGCCGGTAATATGGCAAGTGAGTGGCAAGAAGATGA
ACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTAT
ATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAA

Upstream 100 bases:

>100_bases
ACATTGTTGAAAATATCACGTTAGATAGTATTAACGAAACGTCATCGTTATATTTAAATTTAGATCAGCAAGTCGATAGT
CGTTTGGAGATTAAAAAGTA

Downstream 100 bases:

>100_bases
AATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTGTTA
ATTAAACAAGGGGTGAGGTT

Product: acetoacetyl-CoA reductase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFNDDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQ
SLYGVLQDMKDHDIDACYQLNVLQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQEL
ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNARSVTGTIQKVNGAWYI

Sequences:

>Translated_234_residues
MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFNDDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQ
SLYGVLQDMKDHDIDACYQLNVLQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQEL
ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNARSVTGTIQKVNGAWYI
>Mature_234_residues
MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFNDDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQ
SLYGVLQDMKDHDIDACYQLNVLQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQEL
ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNARSVTGTIQKVNGAWYI

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI40254992, Length=235, Percent_Identity=27.2340425531915, Blast_Score=89, Evalue=2e-18,
Organism=Homo sapiens, GI66933014, Length=216, Percent_Identity=26.3888888888889, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787335, Length=236, Percent_Identity=27.1186440677966, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI2367365, Length=236, Percent_Identity=29.6610169491525, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI87082100, Length=243, Percent_Identity=26.3374485596708, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI87082160, Length=240, Percent_Identity=22.9166666666667, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789208, Length=178, Percent_Identity=25.2808988764045, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789057, Length=184, Percent_Identity=26.0869565217391, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17560676, Length=241, Percent_Identity=27.3858921161826, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17555706, Length=238, Percent_Identity=27.7310924369748, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25147288, Length=243, Percent_Identity=27.5720164609054, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17508651, Length=244, Percent_Identity=26.2295081967213, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322067, Length=205, Percent_Identity=30.2439024390244, Blast_Score=76, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24643142, Length=222, Percent_Identity=23.8738738738739, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI21355319, Length=235, Percent_Identity=27.2340425531915, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24639444, Length=238, Percent_Identity=22.6890756302521, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 25925; Mature: 25925

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFNDDKVRFIQADLSQTID
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEHHHHHHHCC
IDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNVLQLIRLCRYFVDVLRQSA
CHHHHHHHHHCEEEEEECCHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCC
NGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQELALTSVTVNAIAPGFVAGNMA
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHCCCHHCCCHH
SEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNARSVTGTIQKVNGAWYI
HHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCCHHHHHEECCCCCCC
>Mature Secondary Structure
MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFNDDKVRFIQADLSQTID
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEHHHHHHHCC
IDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNVLQLIRLCRYFVDVLRQSA
CHHHHHHHHHCEEEEEECCHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCC
NGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQELALTSVTVNAIAPGFVAGNMA
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHCCCHHCCCHH
SEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNARSVTGTIQKVNGAWYI
HHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCCHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]