Click here to switch to the map view.

The map label for this gene is infB [H]

Identifier: 57651839

GI number: 57651839

Start: 1300186

End: 1302303

Strand: Direct

Name: infB [H]

Synonym: SACOL1288

Alternate gene names: 57651839

Gene position: 1300186-1302303 (Clockwise)

Preceding gene: 57651838

Following gene: 57651840

Centisome position: 46.28

GC content: 34.14

Gene sequence:

>2118_bases
ATGAGTAAACAAAGAATTTACGAATATGCGAAAGAATTAAATCTAAAGAGTAAAGAGATTATAGATGAGTTAAAAAGCAT
GAATATTGAGGTTTCAAATCATATGCAAGCTTTGGAAGATGACCAAATTAAAGCATTAGATAAAAAGTTCAAAAAAGAAC
AAAAGAACGACAATAAACAAAGCACTCAAAATAATCACCAAAAATCAAACAATCAAAACCAAAATAAAGGGCAACAAAAA
GATAACAAAAAGAATCAACAACAAAATAATAAAGGCAACAAAGGCAATAAAAAGAATAATAGAAATAATAAGAAAAATAA
CAAGAATAATAAACCACAAAATCAACCAGCTGCTCCAAAAGAAATACCATCAAAAGTGACATATCAAGAAGGTATTACAG
TAGGCGAATTTGCGGATAAATTAAATGTTGAATCATCAGAAATTATCAAAAAATTATTCTTACTTGGTATTGTTGCTAAT
ATCAATCAATCATTAAATCAAGAAACAATCGAATTAATTGCCGATGATTATGGCGTTGAGGTTGAAGAAGAAGTTGTGAT
TAATGAAGAAGACTTATCAATCTATTTCGAAGACGAAAAAGATGATCCAGAGGCAATTGAGAGACCAGCAGTTGTAACAA
TTATGGGACATGTTGACCATGGTAAAACGACTTTATTAGATTCAATTCGTCATACAAAAGTTACAGCAGGTGAAGCAGGC
GGAATCACTCAACATATTGGTGCATATCAAATTGAAAACGATGGCAAAAAAATCACTTTCTTAGATACACCGGGACATGC
TGCATTTACAACGATGCGTGCGCGTGGTGCACAAGTAACAGATATTACTATTTTAGTAGTAGCAGCTGACGATGGTGTTA
TGCCACAAACAATTGAAGCAATTAACCATGCTAAAGAAGCAGAAGTACCAATTATTGTTGCAGTAAATAAAATTGATAAA
CCAACTTCAAATCCTGATCGAGTTATGCAAGAATTAACTGAATATGGTTTAATTCCTGAAGATTGGGGCGGCGAAACAAT
TTTCGTTCCACTTTCTGCATTAAGTGGTGATGGTATCGACGATTTATTAGAAATGATAGGATTAGTTGCAGAAGTTCAAG
AACTTAAAGCAAATCCTAAAAACCGTGCTGTTGGTACAGTTATCGAAGCTGAATTAGATAAATCACGTGGTCCTTCTGCA
TCATTATTAGTACAAAACGGTACATTAAATGTTGGTGATGCGATTGTAGTTGGTAATACTTACGGCCGTATCCGTGCAAT
GGTTAATGACTTAGGTCAAAGAATCAAAACGGCTGGTCCATCAACGCCTGTTGAAATTACAGGTATTAATGATGTGCCAC
AAGCTGGGGATCGCTTTGTTGTATTTAGTGATGAAAAACAAGCTCGTCGTATTGGTGAATCAAGACACGAAGCTAGCATT
ATACAACAACGTCAAGAAAGTAAAAATGTTTCATTAGATAACCTGTTTGAACAAATGAAACAAGGTGAAATGAAAGATTT
AAACGTTATTATTAAAGGTGATGTTCAAGGTTCTGTTGAAGCTTTAGCTGCATCATTAATGAAAATTGATGTTGAAGGCG
TAAATGTTCGTATCATTCATACAGCGGTTGGTGCAATTAATGAGTCAGACGTGACACTTGCTAATGCCTCAAATGGTATT
ATCATTGGTTTCAATGTTCGTCCAGACAGTGGTGCAAAACGTGCTGCAGAAGCTGAAAATGTTGATATGCGTTTACACAG
AGTTATTTATAATGTTATCGAAGAAATTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCG
GACAAGCTGAAGTTCGTCAAACATTCAAAGTTTCTAAAGTTGGTACTATTGCTGGATGTTATGTTACTGAAGGTAAAATT
ACGCGAAATGCTGGTGTACGTATTATTCGTGATGGTATTGTTCAATATGAAGGCGAATTAGATACACTTAAACGTTTCAA
AGATGATGCTAAGGAAGTTGCAAAAGGTTATGAATGTGGTATTACAATTGAAAACTACAATGACCTTAAAGAAGGCGATG
TTATCGAAGCATTTGAAATGGTTGAAATTAAGCGTTAA

Upstream 100 bases:

>100_bases
GCACTTGGAAAAGGTGAGCGTGTTAATGTAGGGATTACTGACCCAGGCTTTGCTAAAAAGTTGCTATCGATGATAGATGA
ATATCATAAGGAGTGATTAT

Downstream 100 bases:

>100_bases
TTAAATAAATTACAAGCTAAAAGTATAGTTAAGATTGATATGCTCCCTATAAATATTGCACTTTTTAAGTGTCTACTTTA
TAGGGAGCATATTTGATACT

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 705; Mature: 704

Protein sequence:

>705_residues
MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIKALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQK
DNKKNQQQNNKGNKGNKKNNRNNKKNNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHVDHGKTTLLDSIRHTKVTAGEAG
GITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARGAQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDK
PTSNPDRVMQELTEYGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAELDKSRGPSA
SLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGINDVPQAGDRFVVFSDEKQARRIGESRHEASI
IQQRQESKNVSLDNLFEQMKQGEMKDLNVIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGI
IIGFNVRPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKVSKVGTIAGCYVTEGKI
TRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGITIENYNDLKEGDVIEAFEMVEIKR

Sequences:

>Translated_705_residues
MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIKALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQK
DNKKNQQQNNKGNKGNKKNNRNNKKNNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHVDHGKTTLLDSIRHTKVTAGEAG
GITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARGAQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDK
PTSNPDRVMQELTEYGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAELDKSRGPSA
SLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGINDVPQAGDRFVVFSDEKQARRIGESRHEASI
IQQRQESKNVSLDNLFEQMKQGEMKDLNVIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGI
IIGFNVRPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKVSKVGTIAGCYVTEGKI
TRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGITIENYNDLKEGDVIEAFEMVEIKR
>Mature_704_residues
SKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIKALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKD
NKKNQQQNNKGNKGNKKNNRNNKKNNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVANI
NQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHVDHGKTTLLDSIRHTKVTAGEAGG
ITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARGAQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKP
TSNPDRVMQELTEYGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAELDKSRGPSAS
LLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGINDVPQAGDRFVVFSDEKQARRIGESRHEASII
QQRQESKNVSLDNLFEQMKQGEMKDLNVIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGII
IGFNVRPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKVSKVGTIAGCYVTEGKIT
RNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGITIENYNDLKEGDVIEAFEMVEIKR

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family [H]

Homologues:

Organism=Homo sapiens, GI53729339, Length=633, Percent_Identity=37.5987361769352, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI53729337, Length=633, Percent_Identity=37.5987361769352, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI84043963, Length=457, Percent_Identity=26.6958424507659, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI157426893, Length=227, Percent_Identity=28.6343612334802, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1789559, Length=679, Percent_Identity=50.220913107511, Blast_Score=641, Evalue=0.0,
Organism=Escherichia coli, GI1788922, Length=236, Percent_Identity=30.5084745762712, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI2367247, Length=231, Percent_Identity=31.1688311688312, Blast_Score=74, Evalue=5e-14,
Organism=Escherichia coli, GI48994988, Length=170, Percent_Identity=32.3529411764706, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790412, Length=132, Percent_Identity=38.6363636363636, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1789737, Length=132, Percent_Identity=38.6363636363636, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71994658, Length=554, Percent_Identity=34.6570397111913, Blast_Score=308, Evalue=6e-84,
Organism=Caenorhabditis elegans, GI212656558, Length=467, Percent_Identity=26.9807280513919, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17557151, Length=220, Percent_Identity=30.4545454545455, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556456, Length=246, Percent_Identity=31.3008130081301, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324550, Length=636, Percent_Identity=36.4779874213836, Blast_Score=357, Evalue=3e-99,
Organism=Saccharomyces cerevisiae, GI6319282, Length=473, Percent_Identity=27.6955602536998, Blast_Score=124, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6324761, Length=147, Percent_Identity=36.0544217687075, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28572034, Length=531, Percent_Identity=39.1713747645951, Blast_Score=356, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24656849, Length=473, Percent_Identity=27.061310782241, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI160714833, Length=118, Percent_Identity=52.5423728813559, Blast_Score=112, Evalue=1e-24,
Organism=Drosophila melanogaster, GI78706572, Length=225, Percent_Identity=28.4444444444444, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922690, Length=176, Percent_Identity=30.1136363636364, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N [H]

EC number: NA

Molecular weight: Translated: 77872; Mature: 77741

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIKALDKKFKKEQKNDNKQ
CCHHHHHHHHHHCCCCHHHHHHHHHHCCEEHHHHHHHHCCHHHHHHHHHHHHHHCCCCHH
STQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRNNKKNNKNNKPQNQPAAPK
HHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
EIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVANINQSLNQETIELIADDYGVE
HCCCCCHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
VEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHVDHGKTTLLDSIRHTKVTAGEAG
CCCEEEEECCCEEEEEECCCCCHHHHCCCEEEEEEECCCCCHHHHHHHHHHCEECCCCCC
GITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARGAQVTDITILVVAADDGVMPQTIEA
CCHHHCCCEEEECCCCEEEEEECCCCHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHH
INHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTEYGLIPEDWGGETIFVPLSALSGDGID
HHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHH
DLLEMIGLVAEVQELKANPKNRAVGTVIEAELDKSRGPSASLLVQNGTLNVGDAIVVGNT
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCC
YGRIRAMVNDLGQRIKTAGPSTPVEITGINDVPQAGDRFVVFSDEKQARRIGESRHEASI
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEEEECCCHHHHHHCCHHHHHHH
IQQRQESKNVSLDNLFEQMKQGEMKDLNVIIKGDVQGSVEALAASLMKIDVEGVNVRIIH
HHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHEEECCCEEEEEE
TAVGAINESDVTLANASNGIIIGFNVRPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAM
EECCCCCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHH
KGLLDPEFEEQVIGQAEVRQTFKVSKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGEL
HHCCCCCHHHHHCCHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCEEEECCHHHCCCCH
DTLKRFKDDAKEVAKGYECGITIENYNDLKEGDVIEAFEMVEIKR
HHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIKALDKKFKKEQKNDNKQ
CHHHHHHHHHHCCCCHHHHHHHHHHCCEEHHHHHHHHCCHHHHHHHHHHHHHHCCCCHH
STQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRNNKKNNKNNKPQNQPAAPK
HHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
EIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVANINQSLNQETIELIADDYGVE
HCCCCCHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
VEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHVDHGKTTLLDSIRHTKVTAGEAG
CCCEEEEECCCEEEEEECCCCCHHHHCCCEEEEEEECCCCCHHHHHHHHHHCEECCCCCC
GITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARGAQVTDITILVVAADDGVMPQTIEA
CCHHHCCCEEEECCCCEEEEEECCCCHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHH
INHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTEYGLIPEDWGGETIFVPLSALSGDGID
HHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHH
DLLEMIGLVAEVQELKANPKNRAVGTVIEAELDKSRGPSASLLVQNGTLNVGDAIVVGNT
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCC
YGRIRAMVNDLGQRIKTAGPSTPVEITGINDVPQAGDRFVVFSDEKQARRIGESRHEASI
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEEEECCCHHHHHHCCHHHHHHH
IQQRQESKNVSLDNLFEQMKQGEMKDLNVIIKGDVQGSVEALAASLMKIDVEGVNVRIIH
HHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHEEECCCEEEEEE
TAVGAINESDVTLANASNGIIIGFNVRPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAM
EECCCCCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHH
KGLLDPEFEEQVIGQAEVRQTFKVSKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGEL
HHCCCCCHHHHHCCHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCEEEECCHHHCCCCH
DTLKRFKDDAKEVAKGYECGITIENYNDLKEGDVIEAFEMVEIKR
HHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA