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The map label for this gene is proS [H]

Identifier: 57651833

GI number: 57651833

Start: 1291332

End: 1293035

Strand: Direct

Name: proS [H]

Synonym: SACOL1282

Alternate gene names: 57651833

Gene position: 1291332-1293035 (Clockwise)

Preceding gene: 57651832

Following gene: 57651834

Centisome position: 45.96

GC content: 34.86

Gene sequence:

>1704_bases
ATGAAGCAATCCAAAGTTTTTATACCAACGATGCGTGACGTGCCATCAGAAGCAGAAGCACAAAGTCATCGTTTATTATT
GAAATCGGGTTTGATAAAACAAAGTACAAGTGGGATTTATAGTTATTTACCGCTAGCAACACGTGTGTTAAATAATATTA
CTGCAATTGTGCGACAAGAAATGGAACGTATCGATTCTGTTGAAATTTTAATGCCAGCGTTACAACAAGCTGAATTATGG
GAAGAATCAGGACGTTGGGGTGCATATGGCCCAGAATTAATGCGTTTACAAGATAGACATGGAAGACAATTTGCATTAGG
TCCAACACATGAAGAATTAGTTACATCAATAGTAAGAAATGAATTGAAATCATACAAACAATTACCGATGACATTATTCC
AAATTCAATCTAAATTCCGTGATGAAAAGAGACCACGTTTTGGTTTACTTCGTGGGCGTGAATTTATTATGAAAGATGCA
TATTCATTCCATGCTGACGAGGCATCATTAGATCAAACGTATCAAGATATGTATCAAGCGTATAGCCGTATTTTTGAGAG
AGTTGGCATTAACGCAAGACCAGTAGTTGCAGATTCAGGTGCTATAGGCGGTAGCCATACACATGAATTTATGGCATTAA
GTGCTATCGGTGAGGATACAATCGTTTACAGTAAAGAAAGTGATTATGCTGCTAACATCGAAAAAGCAGAAGTCGTTTAC
GAACCAAATCATAAGCATACTACTGTGCAACCTTTAGAAAAAATTGAAACACCAAATGTTAAGACTGCGCAAGAATTGGC
AGACTTCTTAGGTAGACCAGTAGATGAAATCGTTAAAACGATGATTTTCAAAGTTGATGGCGAATATATTATGGTTTTAG
TGCGTGGCCATCATGAAATTAATGACATTAAATTAAAATCTTATTTCGGCACAGATAATATTGAATTAGCAACACAAGAC
GAAATTGTTAATTTAGTTGGTGCAAATCCTGGTTCACTAGGTCCTGTAATTGATAAAGAAATCAAAATTTATGCAGATAA
TTTTGTGCAAGATTTAAATAATTTAGTTGTCGGTGCTAACGAAGATGGTTATCACTTAATTAATGTAAATGTAGGTAGAG
ACTTCAACGTTGATGAATATGGCGATTTCCGTTTTATTTTAGAAGGCGAAAAGTTAAGTGATGGTTCAGGCGCTGCACAT
TTTGCTGAAGGTATTGAAGTTGGTCAAGTATTCAAATTGGGTACTAAGTATTCAGAATCAATGAATGCTACATTCTTAGA
TAACCAAGGAAAAGCTCAATCTTTAATTATGGGTTGTTACGGAATTGGAATTTCTAGAACGCTAAGTGCGATTGTTGAAC
AAAATCACGATGATAATGGAATTGTTTGGCCTAAATCAGTTACTCCGTTTGATTTACATTTAATTTCTATTAATCCTAAG
AAAGATGATCAACGAGAACTAGCAGATGCACTATATGCTGAATTTAATACTAAATTTGATGTGTTGTACGATGATCGTCA
GGAACGTGCAGGTGTTAAATTTAATGATGCCGATTTAATTGGTTTACCACTGCGAATTGTTGTTGGTAAACGTGCATCGG
AAGGTATTGTAGAAGTTAAAGAACGTTTAACAGGTGATAGCGAAGAAGTTCACATTGATGACTTAATGACTGTCATTACA
AATAAATATGATAACTTAAAATAA

Upstream 100 bases:

>100_bases
ATTATTGCTATTGGTGCCATTTTCATGGTCGTTATAATGATATTAGTAACGTGGAATGATATTCGACGATATTTCTTATA
ATTTAGGAGGATAAATAATT

Downstream 100 bases:

>100_bases
TTAAGATCGAATGAATTATAAGAGTAGGAAAAAGCTGAAAGAAATCTGATGCTTATGTCCTGCTCTTATTATTTTTGATA
TAATGATTATTCGATGAAAA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLPLATRVLNNITAIVRQEMERIDSVEILMPALQQAELW
EESGRWGAYGPELMRLQDRHGRQFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGEDTIVYSKESDYAANIEKAEVVY
EPNHKHTTVQPLEKIETPNVKTAQELADFLGRPVDEIVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQD
EIVNLVGANPGSLGPVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKLSDGSGAAH
FAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQSLIMGCYGIGISRTLSAIVEQNHDDNGIVWPKSVTPFDLHLISINPK
KDDQRELADALYAEFNTKFDVLYDDRQERAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVIT
NKYDNLK

Sequences:

>Translated_567_residues
MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLPLATRVLNNITAIVRQEMERIDSVEILMPALQQAELW
EESGRWGAYGPELMRLQDRHGRQFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGEDTIVYSKESDYAANIEKAEVVY
EPNHKHTTVQPLEKIETPNVKTAQELADFLGRPVDEIVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQD
EIVNLVGANPGSLGPVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKLSDGSGAAH
FAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQSLIMGCYGIGISRTLSAIVEQNHDDNGIVWPKSVTPFDLHLISINPK
KDDQRELADALYAEFNTKFDVLYDDRQERAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVIT
NKYDNLK
>Mature_567_residues
MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLPLATRVLNNITAIVRQEMERIDSVEILMPALQQAELW
EESGRWGAYGPELMRLQDRHGRQFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGEDTIVYSKESDYAANIEKAEVVY
EPNHKHTTVQPLEKIETPNVKTAQELADFLGRPVDEIVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQD
EIVNLVGANPGSLGPVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKLSDGSGAAH
FAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQSLIMGCYGIGISRTLSAIVEQNHDDNGIVWPKSVTPFDLHLISINPK
KDDQRELADALYAEFNTKFDVLYDDRQERAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVIT
NKYDNLK

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=230, Percent_Identity=42.6086956521739, Blast_Score=214, Evalue=2e-55,
Organism=Escherichia coli, GI1786392, Length=570, Percent_Identity=44.7368421052632, Blast_Score=520, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI115532348, Length=275, Percent_Identity=36, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI193203271, Length=90, Percent_Identity=34.4444444444444, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=247, Percent_Identity=32.7935222672065, Blast_Score=164, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24656200, Length=248, Percent_Identity=36.2903225806452, Blast_Score=172, Evalue=8e-43,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63836; Mature: 63836

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLPLATRVLNNITAIVRQE
CCCCCEECCCHHCCCCHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGRQFALGPTHEELVTSIVRN
HHHHCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHH
ELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDAYSFHADEASLDQTYQDMYQA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGEDTIVYSKESDYAANIEKAEVVY
HHHHHHHHCCCCCCEEECCCCCCCCCCHHHHEEECCCCCEEEEECCCCCCCCCCCEEEEE
EPNHKHTTVQPLEKIETPNVKTAQELADFLGRPVDEIVKTMIFKVDGEYIMVLVRGHHEI
CCCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHEEECCCEEEEEEECCCCC
NDIKLKSYFGTDNIELATQDEIVNLVGANPGSLGPVIDKEIKIYADNFVQDLNNLVVGAN
CCEEEEEEECCCCEEEEEHHHHHHHCCCCCCCCCCCCCCHHEEEHHHHHHHHHCEEEECC
EDGYHLINVNVGRDFNVDEYGDFRFILEGEKLSDGSGAAHFAEGIEVGQVFKLGTKYSES
CCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHCCHHCCC
MNATFLDNQGKAQSLIMGCYGIGISRTLSAIVEQNHDDNGIVWPKSVTPFDLHLISINPK
CCCEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEECCCCCCCEEEEEEEECCC
KDDQRELADALYAEFNTKFDVLYDDRQERAGVKFNDADLIGLPLRIVVGKRASEGIVEVK
CCHHHHHHHHHHHHCCCCEEEEECCCHHHCCCEECCCCEECCCEEEEECCCCCCCHHHHH
ERLTGDSEEVHIDDLMTVITNKYDNLK
HHHCCCCCEEEHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLPLATRVLNNITAIVRQE
CCCCCEECCCHHCCCCHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGRQFALGPTHEELVTSIVRN
HHHHCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHH
ELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDAYSFHADEASLDQTYQDMYQA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGEDTIVYSKESDYAANIEKAEVVY
HHHHHHHHCCCCCCEEECCCCCCCCCCHHHHEEECCCCCEEEEECCCCCCCCCCCEEEEE
EPNHKHTTVQPLEKIETPNVKTAQELADFLGRPVDEIVKTMIFKVDGEYIMVLVRGHHEI
CCCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHEEECCCEEEEEEECCCCC
NDIKLKSYFGTDNIELATQDEIVNLVGANPGSLGPVIDKEIKIYADNFVQDLNNLVVGAN
CCEEEEEEECCCCEEEEEHHHHHHHCCCCCCCCCCCCCCHHEEEHHHHHHHHHCEEEECC
EDGYHLINVNVGRDFNVDEYGDFRFILEGEKLSDGSGAAHFAEGIEVGQVFKLGTKYSES
CCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHCCHHCCC
MNATFLDNQGKAQSLIMGCYGIGISRTLSAIVEQNHDDNGIVWPKSVTPFDLHLISINPK
CCCEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEECCCCCCCEEEEEEEECCC
KDDQRELADALYAEFNTKFDVLYDDRQERAGVKFNDADLIGLPLRIVVGKRASEGIVEVK
CCHHHHHHHHHHHHCCCCEEEEECCCHHHCCCEECCCCEECCCEEEEECCCCCCCHHHHH
ERLTGDSEEVHIDDLMTVITNKYDNLK
HHHCCCCCEEEHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA