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The map label for this gene is folD [H]

Identifier: 57651672

GI number: 57651672

Start: 1081861

End: 1082721

Strand: Reverse

Name: folD [H]

Synonym: SACOL1072

Alternate gene names: 57651672

Gene position: 1082721-1081861 (Counterclockwise)

Preceding gene: 57651684

Following gene: 57651671

Centisome position: 38.54

GC content: 34.38

Gene sequence:

>861_bases
ATGGTTGCTAAAATTTTAGATGGTAAACAAATTGCCAAAGACTACAGACAGGGGTTACAAGATCAAGTTGAAGCGCTAAA
AGAAAAGGGTTTTACACCTAAATTATCCGTTATATTAGTTGGTAATGATGGCGCTAGTCAAAGTTATGTTAGATCAAAAA
AGAAAGCAGCTGAAAAAATTGGTATGATTTCAGAAATCGTACATTTGGAAGAAACAGCTACTGAAGAAGAAGTATTAAAC
GAACTAAATAGACTAAATAATGATGATTCTGTAAGTGGTATTTTGGTACAAGTACCATTACCAAAACAAGTTAGCGAACA
GAAAATATTAGAAGCAATCAATCCTGAAAAAGATGTGGACGGTTTTCATCCAATAAATATAGGGAAATTATATATCGATG
AACAAACTTTTGTACCTTGCACACCGCTCGGCATCATGGAAATATTAAAACATGCTGATATTGATTTAGAAGGTAAAAAT
GCAGTTGTAATTGGACGAAGTCATATTGTCGGACAACCAGTTTCTAAGTTACTACTTCAAAAAAATGCATCAGTAACAAT
CTTACATTCTCGTTCAAAAGATATGGCATCATATTTAAAAGATGCTGATGTCATTGTCAGTGCAGTTGGTAAGCCTGGTT
TAGTAACAAAAGATGTGGTCAAAGAAGGAGCAGTAATTATCGATGTTGGCAATACGCCAGATGAAAATGGCAAATTAAAA
GGTGACGTTGATTATGATGCGGTTAAAGAAATTGCTGGAGCTATTACACCAGTTCCTGGTGGCGTTGGTCCATTAACAAT
TACTATGGTATTAAATAATACTTTGCTTGCAGAAAAAATGCGTCGAGGTATTGATTCGTAA

Upstream 100 bases:

>100_bases
CTTTATTTTGATTTTTTGTTTGAATTTACGTCTTTTGCTATGTAAATTAGTTTTATAAACTAACAAAGTTAGGATATTGA
CAATAGGAGGAGAAGTTTTT

Downstream 100 bases:

>100_bases
AGAGCCTGAGACATAAATCAATGTTCTATGCTCTACAAAGTTATAATGGCAGTAGTTGAGTGAACGAAAATTCGCTTGTA
ACAAGCTTTTTTCAATTCTA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDGASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLN
ELNRLNNDDSVSGILVQVPLPKQVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKDVVKEGAVIIDVGNTPDENGKLK
GDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLLAEKMRRGIDS

Sequences:

>Translated_286_residues
MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDGASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLN
ELNRLNNDDSVSGILVQVPLPKQVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKDVVKEGAVIIDVGNTPDENGKLK
GDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLLAEKMRRGIDS
>Mature_286_residues
MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDGASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLN
ELNRLNNDDSVSGILVQVPLPKQVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKDVVKEGAVIIDVGNTPDENGKLK
GDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLLAEKMRRGIDS

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=45.7337883959044, Blast_Score=234, Evalue=5e-62,
Organism=Homo sapiens, GI222136639, Length=289, Percent_Identity=43.9446366782007, Blast_Score=231, Evalue=4e-61,
Organism=Homo sapiens, GI94721354, Length=293, Percent_Identity=43.6860068259386, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI36796743, Length=226, Percent_Identity=26.9911504424779, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1786741, Length=278, Percent_Identity=47.1223021582734, Blast_Score=258, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI17568735, Length=295, Percent_Identity=37.2881355932203, Blast_Score=197, Evalue=8e-51,
Organism=Saccharomyces cerevisiae, GI6321643, Length=297, Percent_Identity=43.7710437710438, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6319558, Length=292, Percent_Identity=44.5205479452055, Blast_Score=243, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6322933, Length=314, Percent_Identity=28.0254777070064, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=43.8775510204082, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=43.8775510204082, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI62472483, Length=284, Percent_Identity=41.5492957746479, Blast_Score=222, Evalue=2e-58,
Organism=Drosophila melanogaster, GI45551871, Length=284, Percent_Identity=41.5492957746479, Blast_Score=222, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24645718, Length=284, Percent_Identity=41.5492957746479, Blast_Score=222, Evalue=3e-58,
Organism=Drosophila melanogaster, GI17137370, Length=284, Percent_Identity=41.5492957746479, Blast_Score=222, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30844; Mature: 30844

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDGASQSYVRSKKKAAEKI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
GMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPKQVSEQKILEAINPEKDVD
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCC
GFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKNAVVIGRSHIVGQPVSKLLLQ
CCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCHHCCCHHHHHHHH
KNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKDVVKEGAVIIDVGNTPDENGKLK
CCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEECCCCCCCCCCEE
GDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLLAEKMRRGIDS
CCCCHHHHHHHHHCCCCCCCCCCCEEEEEHHCCHHHHHHHHHCCCC
>Mature Secondary Structure
MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDGASQSYVRSKKKAAEKI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
GMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPKQVSEQKILEAINPEKDVD
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCC
GFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKNAVVIGRSHIVGQPVSKLLLQ
CCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCHHCCCHHHHHHHH
KNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKDVVKEGAVIIDVGNTPDENGKLK
CCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEECCCCCCCCCCEE
GDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLLAEKMRRGIDS
CCCCHHHHHHHHHCCCCCCCCCCCEEEEEHHCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA