Definition | Staphylococcus aureus subsp. aureus COL chromosome, complete genome. |
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Accession | NC_002951 |
Length | 2,809,422 |
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The map label for this gene is mnhA [H]
Identifier: 57651643
GI number: 57651643
Start: 955163
End: 957568
Strand: Reverse
Name: mnhA [H]
Synonym: SACOL0955
Alternate gene names: 57651643
Gene position: 957568-955163 (Counterclockwise)
Preceding gene: 57651644
Following gene: 57651642
Centisome position: 34.08
GC content: 33.46
Gene sequence:
>2406_bases GTGAGTTTGTTACATATTGCGGTGATATTACCGTTAATATTCGCACTCATTATTCCTATCCTGTATAGATTTTTTAAACG TATACATTTAGGATGGTTCGTATTACCTGTCCCTATCGTCATATTTATTTATATGCTTACATTAATTAAAACGACGATGT CAGGTAATACTGTAATGAAAACCTTAAATTGGATGCCTCATTTTGGTATGAACTTTGATTTATATCTTGATGGCTTAGGT TTACTCTTTAGTTTGCTTATTTCAGGAATCGGCAGTTTAGTAGTATTATATTCAATCGGCTACTTAAGTAAGTCAGAACA ACTAGGGAATTTTTATTGTTATTTATTACTATTTATGGGTGCAATGTTAGGTGTCGTACTTTCAGATAATGTTATTATTC TATATTTATTCTGGGAGCTAACGTCATTTTCTAGTTTTTTACTTATTTCATTCTGGCGCGAACGACAAGCATCTATATAT GGTGCTCAAAAATCGCTCATAATCACAGTATTTGGTGGTCTATCATTATTAGGTGGTATCATCTTACTCGCTATACCAAC ACAATCATTTAGCATTCAGTACATGATTCAACATGCTTCTGAAATTCAAAATAGCCCATTCTTCATTTTTGCGATGATTT TAATCATGATTGGTGCTTTTACAAAATCTGCTCAGTTTCCATTTTATATTTGGCTACCAGATGCAATGGAAGCACCTACA CCAGTCAGTGCTTATCTTCATTCAGCAACAATGGTTAAAGCAGGGTTATATTTAATCGCTCGAATGACACCAATCTTCGC AGCATCGCAAGGTTGGGTATGGACTGTCACACTCGTTGGTTTAATTACATTATTCTGGGCTTCGCTAAACGCAACAAAAC AACAAGACTTGAAAGGTATACTGGCATTTTCAACAGTATCCCAATTAGGTATGATTATGGCGATGCTTGGAATCGGTGCC ATTAGTTATCACTATCAAGGCGATGATAGCAAAATATATGCTGCTGCATTTACGGCTGCAATATTCCATTTAATCAACCA TGCAACATTTAAAGGTGCCCTATTTATGATTACAGGTGCAGTGGATCATTCTACTGGTACACGTGATGTTAAGAAATTAG GTGGCTTACTAACAATTATGCCTATTTCATTTACAATCACTGTTATTACAGCATTAAGTATGGCTGGTGTACCACCGTTC AATGGTTTCTTATCAAAAGAATCATTTTTAGAAACAACATTTACAGCAAGCCAAGCAAATTTATTCAGTGTTGATACATT AGGCTACCTATTCCCAATCATTGGTATAGTGGGCAGTGTCTTCACATTTGTATATTCAATTAAATTTATAATGCATATAT TTTTCGGACAATATAAACCTGAACAATTACCTAAAAAAGCACACGAAGTATCAATATTAATGCTTTTATCACCAGCTATT TTAGCTACATTAGTAATTGTATTTGGTTTATTCCCTGGCATTTTAACTAATTCGATTATTGAACCAGCTACATCTTCGAT TAATCATACGGTAATTGATGATGTTGAATTCCATATGTTCCATGGATTAACGCCTGCCTTTTTATCAACATTAGTGATTT ATATTTTAGGTATCTTACTAATTGTGACATTTAGTTATTGGGTGAAATTGTTGCAACGACAACCTGGTAAACTAACATTC AATTATTGGTACAACAGAAGTGCAAATGTTATTCCAAACTACTCTGAAAAGATGACGAATAGCTATGTTACCGATTATTC ACGTAATAATTTAGTTATCATTTTTGGTGCATTAATTTTATTAACATTTGTCACTATATTCTCAGTACCTTTCAACATTA ATTTTAAAGACGTTAGTCCGATTAGAATTTTCGAAGTTTGTATCGTAATTTTATTATTAAGTGCTGCCTTTTTAATTCTT TTCGCTAAATCAAGATTATTTAGCATCATTATGTTAAGTGCAGTTGGATATGCTGTGTCTGTATTGTTTATATTCTTTAA AGCACCCGACTTAGCATTGACACAGTTTGTCGTTGAATCTATTTCAACAGCATTGTTCTTATTATGCTTCTACCACTTAC CAAATTTAAATCGTTATAATGAAAAGCGCTCATTCCAGCTTACTAATGCTTTAATTGCTGGTGGTGTTGGTTTGTCCGTC ATTATTATCGGTTTAATTGCATACGGAAATAGACATTTTGAATCGATATCTAAGTTTTATCAAGAGCACGTTTATGATTT AGCTCACGGTAAAAACATGGTAAACGTCATACTTGTTGATTTCCGTGGTATGGATACTTTATTCGAGTCATCTGTGCTTG GTATCGCAGGATTAGCTGTATATACAATGATTAAATTACGTAAAAAGCGTCAAACGCAAGGTAACGAGGTGAAAAATCAT GAATAG
Upstream 100 bases:
>100_bases TTCTGAAAATAAAGCACAACAAGGAGTGTGTACGTTTTTCAGAACTATATCATTCATGTATAATGAATAACATAAATATT TTTTAAAGGAGGACAACATA
Downstream 100 bases:
>100_bases ACAACAAAATGATCTAATTTTGCAATTTGCTGCAGTTATTATCTTTTTCATGGTAATGGTCTTTGGTTTTTCACTTTTCT TAGCAGGACATTATACACCT
Product: monovalent cation/H+ antiporter subunit A
Products: NAD+; ubiquinol [C]
Alternate protein names: Mnh complex subunit A1 [H]
Number of amino acids: Translated: 801; Mature: 800
Protein sequence:
>801_residues MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIYMLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLG LLFSLLISGIGSLVVLYSIGYLSKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIGAFTKSAQFPFYIWLPDAMEAPT PVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTVTLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGA ISYHYQGDDSKIYAAAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITALSMAGVPPF NGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFIMHIFFGQYKPEQLPKKAHEVSILMLLSPAI LATLVIVFGLFPGILTNSIIEPATSSINHTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTF NYWYNRSANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIFEVCIVILLLSAAFLIL FAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESISTALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSV IIIGLIAYGNRHFESISKFYQEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVKNH E
Sequences:
>Translated_801_residues MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIYMLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLG LLFSLLISGIGSLVVLYSIGYLSKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIGAFTKSAQFPFYIWLPDAMEAPT PVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTVTLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGA ISYHYQGDDSKIYAAAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITALSMAGVPPF NGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFIMHIFFGQYKPEQLPKKAHEVSILMLLSPAI LATLVIVFGLFPGILTNSIIEPATSSINHTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTF NYWYNRSANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIFEVCIVILLLSAAFLIL FAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESISTALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSV IIIGLIAYGNRHFESISKFYQEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVKNH E >Mature_800_residues SLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIYMLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGL LFSLLISGIGSLVVLYSIGYLSKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIYG AQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIGAFTKSAQFPFYIWLPDAMEAPTP VSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTVTLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAI SYHYQGDDSKIYAAAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITALSMAGVPPFN GFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFIMHIFFGQYKPEQLPKKAHEVSILMLLSPAIL ATLVIVFGLFPGILTNSIIEPATSSINHTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFN YWYNRSANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIFEVCIVILLLSAAFLILF AKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESISTALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVI IIGLIAYGNRHFESISKFYQEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVKNHE
Specific function: Mnh complex is a Na(+)/H(+) antiporter involved in Na(+) excretion [H]
COG id: COG1009
COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit
Gene ontology:
Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]
Homologues:
Organism=Homo sapiens, GI251831117, Length=473, Percent_Identity=29.8097251585624, Blast_Score=178, Evalue=2e-44, Organism=Escherichia coli, GI1788614, Length=522, Percent_Identity=30.2681992337165, Blast_Score=206, Evalue=5e-54, Organism=Escherichia coli, GI1788829, Length=477, Percent_Identity=32.4947589098532, Blast_Score=184, Evalue=2e-47, Organism=Escherichia coli, GI1788831, Length=358, Percent_Identity=32.4022346368715, Blast_Score=145, Evalue=1e-35, Organism=Escherichia coli, GI1788827, Length=445, Percent_Identity=26.7415730337079, Blast_Score=125, Evalue=8e-30, Organism=Escherichia coli, GI1788613, Length=466, Percent_Identity=25.9656652360515, Blast_Score=119, Evalue=9e-28, Organism=Escherichia coli, GI2367154, Length=458, Percent_Identity=24.0174672489083, Blast_Score=95, Evalue=1e-20,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR005663 - InterPro: IPR001750 - InterPro: IPR001516 - InterPro: IPR018393 [H]
Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]
EC number: 1.6.99.5 [C]
Molecular weight: Translated: 89398; Mature: 89266
Theoretical pI: Translated: 9.39; Mature: 9.39
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 3.5 %Met (Translated Protein) 3.9 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 3.4 %Met (Mature Protein) 3.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIYMLTLIKTTMSGNTVMK CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH TLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYLSKSEQLGNFYCYLLLFMG HHHCCCCCCCCHHHEECHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH AMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIYGAQKSLIITVFGGLSLLGGI HHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHH ILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIGAFTKSAQFPFYIWLPDAMEAPT HEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCC PVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTVTLVGLITLFWASLNATKQQDLKGI CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH LAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYAAAFTAAIFHLINHATFKGALFMITGA HHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEHHHHHHHHHHHHHHHHHHCCEEEEEEEC VDHSTGTRDVKKLGGLLTIMPISFTITVITALSMAGVPPFNGFLSKESFLETTFTASQAN CCCCCCHHHHHHHCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCC LFSVDTLGYLFPIIGIVGSVFTFVYSIKFIMHIFFGQYKPEQLPKKAHEVSILMLLSPAI EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHH LATLVIVFGLFPGILTNSIIEPATSSINHTVIDDVEFHMFHGLTPAFLSTLVIYILGILL HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHH IVTFSYWVKLLQRQPGKLTFNYWYNRSANVIPNYSEKMTNSYVTDYSRNNLVIIFGALIL HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCEEEHHHHHHH LTFVTIFSVPFNINFKDVSPIRIFEVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVS HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH VLFIFFKAPDLALTQFVVESISTALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSV HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHH IIIGLIAYGNRHFESISKFYQEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAV HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHH YTMIKLRKKRQTQGNEVKNHE HHHHHHHHHHCCCCCCCCCCC >Mature Secondary Structure SLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIYMLTLIKTTMSGNTVMK CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH TLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYLSKSEQLGNFYCYLLLFMG HHHCCCCCCCCHHHEECHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH AMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIYGAQKSLIITVFGGLSLLGGI HHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHH ILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIGAFTKSAQFPFYIWLPDAMEAPT HEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCC PVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTVTLVGLITLFWASLNATKQQDLKGI CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH LAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYAAAFTAAIFHLINHATFKGALFMITGA HHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEHHHHHHHHHHHHHHHHHHCCEEEEEEEC VDHSTGTRDVKKLGGLLTIMPISFTITVITALSMAGVPPFNGFLSKESFLETTFTASQAN CCCCCCHHHHHHHCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCC LFSVDTLGYLFPIIGIVGSVFTFVYSIKFIMHIFFGQYKPEQLPKKAHEVSILMLLSPAI EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHH LATLVIVFGLFPGILTNSIIEPATSSINHTVIDDVEFHMFHGLTPAFLSTLVIYILGILL HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHH IVTFSYWVKLLQRQPGKLTFNYWYNRSANVIPNYSEKMTNSYVTDYSRNNLVIIFGALIL HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCEEEHHHHHHH LTFVTIFSVPFNINFKDVSPIRIFEVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVS HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH VLFIFFKAPDLALTQFVVESISTALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSV HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHH IIIGLIAYGNRHFESISKFYQEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAV HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHH YTMIKLRKKRQTQGNEVKNHE HHHHHHHHHHCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: Iron [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NADH; ubiquinone [C]
Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]
General reaction: Redox reaction [C]
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: NA