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The map label for this gene is proP

Identifier: 57651449

GI number: 57651449

Start: 652802

End: 654202

Strand: Direct

Name: proP

Synonym: SACOL0620

Alternate gene names: 57651449

Gene position: 652802-654202 (Clockwise)

Preceding gene: 57651448

Following gene: 57651450

Centisome position: 23.24

GC content: 34.33

Gene sequence:

>1401_bases
ATGGATTTTAATAAAGAGAATATTAACATGGTGGATGCAAAGAAAGCTAAAAAAACCGTTGTTGCAACCGGTATCGGTAA
TGCAATGGAATGGTTCGATTTTGGTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGA
ATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTT
GGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCAT
TGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACTATTATTGCTTGCAAGAGTACTACAAGGGTTTT
CAACAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGT
GGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGA
ACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTA
AGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACTTTTTACAAATCATC
AGATTTTATTACAAAGATATATTTGTATGTTTTGTAGCTGTTGTATTCTTCAATGTTACAAACTATATGGTAACTGCATA
TTTACCAACCTATTTAGAACAAGTTATTAAATTAGATGCAACGACAACAAGTGTATTAATTACTTGTGTCATGGCAATAA
TGATTCCATTAGCATTAATGTTTGGTAAGTTAGCGGATAAAATAGGTGAAAAGAAAGTATTTCTAATTGGTACTGGTGGG
CTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATT
AGGATTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAA
CTTTATCAGTAACATTTAATATCTCTGTTTCGATATTTGGTGGTACGACGCCATTAGTTGCAACATGGTTAGTTACGAAA
ACTGGAGATCCATTAGCACCTGCGTATTATTTAACAGCAATCAGTGTTATTGGCTTTTTAGTTATTACATTCTTACATTT
AAGTACAGCAGGAAAATCTCTAAAAGGTTCGTATCCAAATGTAGATAACGAGCAAGATAGAGCTTATTATGCAGAACATC
CAAAAGAAGCATTATGGTGGGTTAAAGAACGTAAGAATTAG

Upstream 100 bases:

>100_bases
ATACTTTTGTATTTATATAAAAGTTTAAATTCTTATAAATTTGACAAAACTAATTAACTCCGTATAATTATGAAACATAC
AAGAGGGAGTGTATGAATTC

Downstream 100 bases:

>100_bases
AGATTTTAATAAAAAGTATAAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTT
AAATTGAGAACTGTAATTAG

Product: osmoprotectant proline transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYIGANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVF
GIIGDKYGRKVVLTSTIILMAFSTLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEESPVFENDVATQPERDNINFLQII
RFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVIKLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGG
LTLFSIIAFMLLHSQSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPLVATWLVTK
TGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYYAEHPKEALWWVKERKN

Sequences:

>Translated_466_residues
MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYIGANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVF
GIIGDKYGRKVVLTSTIILMAFSTLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEESPVFENDVATQPERDNINFLQII
RFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVIKLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGG
LTLFSIIAFMLLHSQSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPLVATWLVTK
TGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYYAEHPKEALWWVKERKN
>Mature_466_residues
MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYIGANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVF
GIIGDKYGRKVVLTSTIILMAFSTLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEESPVFENDVATQPERDNINFLQII
RFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVIKLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGG
LTLFSIIAFMLLHSQSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPLVATWLVTK
TGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYYAEHPKEALWWVKERKN

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family

Homologues:

Organism=Escherichia coli, GI1790550, Length=457, Percent_Identity=37.855579868709, Blast_Score=341, Evalue=7e-95,
Organism=Escherichia coli, GI1788292, Length=421, Percent_Identity=30.8788598574822, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1788942, Length=430, Percent_Identity=30.6976744186047, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI1789941, Length=397, Percent_Identity=33.5012594458438, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI87082404, Length=398, Percent_Identity=26.8844221105528, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI87082231, Length=438, Percent_Identity=23.0593607305936, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROP_STAA1 (Q9KWK6)

Other databases:

- EMBL:   AP009324
- EMBL:   AB035449
- RefSeq:   YP_001441161.1
- ProteinModelPortal:   Q9KWK6
- STRING:   Q9KWK6
- EnsemblBacteria:   EBSTAT00000004529
- GeneID:   5559503
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_0571
- eggNOG:   COG0477
- GeneTree:   EBGT00050000023928
- HOGENOM:   HBG757988
- OMA:   LHFNETH
- ProtClustDB:   CLSK2301393
- BioCyc:   SAUR418127:SAHV_0571-MONOMER
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 51658; Mature: 51658

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS50850 MFS; PS00216 SUGAR_TRANSPORT_1; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc8eb260)-; HASH(0xb513ecc)-; HASH(0xc48dd0c)-; HASH(0xc5b56a0)-; HASH(0xd17c69c)-; HASH(0xd432c14)-; HASH(0xd77dff0)-; HASH(0xd2746d0)-; HASH(0xc494610)-; HASH(0xc432164)-; HASH(0xcaa8324)-; HASH(0xd491abc)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYIGANFFSPVENADIRQM
CCCCCCCCCCCCHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCCHHHHH
LTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAFSTLTIGLLPSYDQIGLWA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
PILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGSGLEIGTLSGYIAASIMIAVL
HHHHHHHHHHHCCCCCCCHHHHHHEEECCCCHHHHHHCCCCCEECHHHHHHHHHHHHHHH
TFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEESPVFENDVATQPERDNINFLQII
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
RFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVIKLDATTTSVLITCVMAIMIPLALM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
FGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHSQSFVVIVIGIFILGFFLSTYEATMPG
HHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCC
SLPTMFYSHIRYRTLSVTFNISVSIFGGTTPLVATWLVTKTGDPLAPAYYLTAISVIGFL
CHHHHHHHHHHEEEEEEEEEEEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
VITFLHLSTAGKSLKGSYPNVDNEQDRAYYAEHPKEALWWVKERKN
HHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCH
>Mature Secondary Structure
MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYIGANFFSPVENADIRQM
CCCCCCCCCCCCHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCCHHHHH
LTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAFSTLTIGLLPSYDQIGLWA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
PILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGSGLEIGTLSGYIAASIMIAVL
HHHHHHHHHHHCCCCCCCHHHHHHEEECCCCHHHHHHCCCCCEECHHHHHHHHHHHHHHH
TFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEESPVFENDVATQPERDNINFLQII
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
RFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVIKLDATTTSVLITCVMAIMIPLALM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
FGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHSQSFVVIVIGIFILGFFLSTYEATMPG
HHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCC
SLPTMFYSHIRYRTLSVTFNISVSIFGGTTPLVATWLVTKTGDPLAPAYYLTAISVIGFL
CHHHHHHHHHHEEEEEEEEEEEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
VITFLHLSTAGKSLKGSYPNVDNEQDRAYYAEHPKEALWWVKERKN
HHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10708580