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The map label for this gene is gltX [H]

Identifier: 57651404

GI number: 57651404

Start: 596014

End: 597468

Strand: Direct

Name: gltX [H]

Synonym: SACOL0574

Alternate gene names: 57651404

Gene position: 596014-597468 (Clockwise)

Preceding gene: 57651403

Following gene: 57651405

Centisome position: 21.21

GC content: 34.16

Gene sequence:

>1455_bases
ATGAGCGATCGTATAAGAGTAAGATATGCACCAAGTCCAACTGGGTATCTTCATATTGGTAATGCAAGAACAGCATTATT
CAATTACTTGTATGCTAAACATTACAACGGAGATTTTGTGATTCGAATTGAAGATACTGATAAAAAACGTAATTTAGAAG
ATGGAGAAACATCACAATTTGATAATCTTAAATGGTTAGGATTAGATTGGGATGAGTCTGTAGATAAAGACAATGGCTAC
GGACCATATCGTCAATCTGAACGTCAACATATCTACCAACCATTAATAGATCAGTTACTAGCAGAAGATAAAGCATATAA
ATGCTATATGACAGAAGAAGAATTAGAAGCTGAACGTGAAGCGCAAATCGCTCGTGGTGAAATGCCTCGCTATGGTGGTC
AACATGCGCATTTGACTGAAGAACAACGTCAACAATTTGAAGCAGAAGGACGCCAACCATCAATTCGTTTCCGAGTACCT
CAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTAT
CGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTC
GTGGTGATGATCATATTTCAAACACGCCTAAACAAATTATGATTTATGAAGCATTTGGCTGGGAGCCACCTCGTTTTGGT
CATATGTCATTAATTGTTAATGAAGAACGTAAAAAGTTAAGTAAACGTGATGGGCAAATTTTACAATTTATTGAGCAATA
TCGTGACTTAGGTTATTTACCTGAAGCGTTATTTAATTTTATTGCGTTATTAGGTTGGTCTCCTGAAGGTGAAGAAGAAA
TCTTTTCTAAAGAAGAATTTATCAAAATCTTTGATGAAAAGCGTTTGTCAAAATCACCAGCATTTTTCGATAAGCAAAAA
TTAGCATGGGTTAATAACCAATATATGAAACAAAAAGATACTGAAACAGTATTCCAATTAGCATTACCTCATTTAATTAA
AGCAAATTTGATTCCTGAGGTGCCGTCAGAAGAGGATTTATCTTGGGGACGCAAATTAATTGCGCTTTATCAAAAAGAAA
TGAGTTATGCCGGTGAAATTGTACCTTTATCAGAAATGTTCTTTAAAGAAATGCCAGCTCTTGGTGAAGAAGAACAACAA
GTGATTAATGGAAAGCAAGTACCAGAGTTAATGACGCACTTATTCAGTAAATTAGAAGCACTTGAACCATTTGAATCGGC
TGAAATTAAAAAGACAATTAAAGAAGTTCAAAAAGAAACAGGAATAAAAGGCAAGCAATTATTTATGCCTATTCGTGTTG
CTGTAACAGGCCAAATGCATGGTCCTGAATTACCAAATACAATTGAAGTACTTGGTAAAGAAAAAGTGCTAAACCGTTTA
AAACAATATAAGTAA

Upstream 100 bases:

>100_bases
TAAGACGAGTTAGACATTTGTTATGACGGTCGAAGATAGCAAATGAACAATTTATATGAACTAACTTGAAAATCTGATAT
TTAATTTAGGAGTGAAAAAA

Downstream 100 bases:

>100_bases
TGAAATAGACAAGATCAAGGTAGTATATACTTGAATTTTATAACGTAACCACATATGATAAAGTTGTATTAACAAATTAC
AACAATATGAATAAAGTATT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIEDTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGY
GPYRQSERQHIYQPLIDQLLAEDKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDHISNTPKQIMIYEAFGWEPPRFG
HMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEALFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQK
LAWVNNQYMKQKDTETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMPALGEEEQQ
VINGKQVPELMTHLFSKLEALEPFESAEIKKTIKEVQKETGIKGKQLFMPIRVAVTGQMHGPELPNTIEVLGKEKVLNRL
KQYK

Sequences:

>Translated_484_residues
MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIEDTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGY
GPYRQSERQHIYQPLIDQLLAEDKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDHISNTPKQIMIYEAFGWEPPRFG
HMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEALFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQK
LAWVNNQYMKQKDTETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMPALGEEEQQ
VINGKQVPELMTHLFSKLEALEPFESAEIKKTIKEVQKETGIKGKQLFMPIRVAVTGQMHGPELPNTIEVLGKEKVLNRL
KQYK
>Mature_483_residues
SDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIEDTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYG
PYRQSERQHIYQPLIDQLLAEDKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVPQ
NQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDHISNTPKQIMIYEAFGWEPPRFGH
MSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEALFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKL
AWVNNQYMKQKDTETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMPALGEEEQQV
INGKQVPELMTHLFSKLEALEPFESAEIKKTIKEVQKETGIKGKQLFMPIRVAVTGQMHGPELPNTIEVLGKEKVLNRLK
QYK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=491, Percent_Identity=30.9572301425662, Blast_Score=253, Evalue=4e-67,
Organism=Escherichia coli, GI1788743, Length=480, Percent_Identity=38.75, Blast_Score=330, Evalue=2e-91,
Organism=Escherichia coli, GI226510925, Length=251, Percent_Identity=29.8804780876494, Blast_Score=122, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI71993394, Length=488, Percent_Identity=32.7868852459016, Blast_Score=241, Evalue=7e-64,
Organism=Saccharomyces cerevisiae, GI6324540, Length=501, Percent_Identity=32.9341317365269, Blast_Score=263, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6321192, Length=213, Percent_Identity=25.8215962441315, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324742, Length=146, Percent_Identity=31.5068493150685, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24665321, Length=497, Percent_Identity=32.9979879275654, Blast_Score=275, Evalue=5e-74,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 56305; Mature: 56173

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIEDTDKKRNLEDGETSQF
CCCCEEEEECCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEEECCCCCCCCCCCCCCCC
DNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAEDKAYKCYMTEEELEAERE
CCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHH
AQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVPQNQTYSFDDMVKGNISFDSN
HHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHCCCCCCCCC
GIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDHISNTPKQIMIYEAFGWEPPRFG
CCCCEEEEECCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCC
HMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEALFNFIALLGWSPEGEEEIFSKEEF
CEEEEECHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
IKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDTETVFQLALPHLIKANLIPEVPSEEDL
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
SWGRKLIALYQKEMSYAGEIVPLSEMFFKEMPALGEEEQQVINGKQVPELMTHLFSKLEA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHH
LEPFESAEIKKTIKEVQKETGIKGKQLFMPIRVAVTGQMHGPELPNTIEVLGKEKVLNRL
HCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
KQYK
HCCC
>Mature Secondary Structure 
SDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIEDTDKKRNLEDGETSQF
CCCEEEEECCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEEECCCCCCCCCCCCCCCC
DNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAEDKAYKCYMTEEELEAERE
CCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHH
AQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVPQNQTYSFDDMVKGNISFDSN
HHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHCCCCCCCCC
GIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDHISNTPKQIMIYEAFGWEPPRFG
CCCCEEEEECCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCC
HMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEALFNFIALLGWSPEGEEEIFSKEEF
CEEEEECHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
IKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDTETVFQLALPHLIKANLIPEVPSEEDL
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
SWGRKLIALYQKEMSYAGEIVPLSEMFFKEMPALGEEEQQVINGKQVPELMTHLFSKLEA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHH
LEPFESAEIKKTIKEVQKETGIKGKQLFMPIRVAVTGQMHGPELPNTIEVLGKEKVLNRL
HCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
KQYK
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA