Click here to switch to the map view.

The map label for this gene is tilS

Identifier: 57651385

GI number: 57651385

Start: 561623

End: 562918

Strand: Direct

Name: tilS

Synonym: SACOL0553

Alternate gene names: 57651385

Gene position: 561623-562918 (Clockwise)

Preceding gene: 57651384

Following gene: 57651386

Centisome position: 19.99

GC content: 30.79

Gene sequence:

>1296_bases
ATGCAGTTAAATAGTAATGGTTGGCATGTTGATGACCATATTGTTGTCGCTGTTTCTACAGGTATTGATAGTATGTGTTT
ATTGTATCAACTACTAAATGATTATAAAGATAGTTATAGAAAACTAACATGCTTACATGTCAATCATGGCGTTAGGTCAG
CTTCAATTGAGGAAGCCAGATTTTTAGAAGCATACTGCGAACGTCATCACATCGATTTACATATCAAAAAGTTAGATTTG
TCGCATAGTCTCGACCGAAATAACAGCATTCAGAATGAAGCTCGAATTAAACGTTACGAATGGTTTGATGAAATGATGAA
TGTATTAGAAGCGGATGTATTGCTAACGGCGCATCATTTGGACGATCAATTAGAAACTATTATGTATCGTATTTTTAATG
GGAAATCAACACGTAATAAACTAGGATTTGATGAGTTATCGAAGCGAAAAGGTTATCAGATTTATCGACCACTTTTAGCT
GTCTCTAAAAAAGAAATAAAACAATTCCAAGAGAGATATCATATTCCATATTTTGAAGATGAATCTAATAAAGATAACAA
ATATGTTAGAAATGATATTCGTAATAGAATTATTCCAGCTATTGATGAAAATAATCAACTTAAAGTATCGCATTTATTAA
AATTAAAACAATGGCATGATGAACAATATGATATTTTGCAATATTCAGCTAAACAATTTATTCAAGAATTTGTGAAGTTT
GATGAACAGTCAAAATATTTAGAGGTTTCTAGACAAGCTTTTAATAACTTACCAAACTCATTAAAGATGGTTGTGTTGGA
CTGCCTATTATCAAAGTATTATGAGTTGTTTAATATTAGTGCTAAAACATACGAAGAGTGGTTTAAACAATTTAGTAGTA
AGAAAGCACAATTCAGTATTAATCTCACGGATAAATGGATAATTCAAATCGCATATGGTAAATTAATAATAATGGCTAAA
AATAATGGCGATACATATTTTAGAGTTCAAACAATTAAAAAGCCAGGTAATTATATTTTTAACAAATATCGATTAGAGAT
ACATTCTAATTTACCAAAATGTTTATTTCCGCTTACAGTGAGAACACGACAAAGTGGCGATACATTTAAACTGAATGGGC
GCGATGGTTATAAGAAAGTGAATCGCCTGTTTATAGATTGTAAAGTGCCACAGTGGGTTCGGGATCAAATGCCAATCGTA
TTGGATAAACAACAGCGCATTATTGCGGTAGGAGATTTATATCAACAACAAACAATAAAAAAATGGATTATAATTAGTAA
AAATGGAGATGAATAG

Upstream 100 bases:

>100_bases
ATCTAAGACTGTTTCTTAAATAAGGAGCAGTCTCTTTTATTTGTAATGATATAACTAAGACTTATACCATTTTTGAAAAT
TGTAAAAGTGAGGTGATGTT

Downstream 100 bases:

>100_bases
CGTTATGCATAATGATTTGAAAGAAGTATTGTTAACTGAAGAAGATATTCAAAATATCTGTAAGGAATTGGGAGCACAAT
TAACAAAGGATTATCAAGGT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTCLHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDL
SHSLDRNNSIQNEARIKRYEWFDEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQWHDEQYDILQYSAKQFIQEFVKF
DEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYELFNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAK
NNGDTYFRVQTIKKPGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQWVRDQMPIV
LDKQQRIIAVGDLYQQQTIKKWIIISKNGDE

Sequences:

>Translated_431_residues
MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTCLHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDL
SHSLDRNNSIQNEARIKRYEWFDEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQWHDEQYDILQYSAKQFIQEFVKF
DEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYELFNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAK
NNGDTYFRVQTIKKPGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQWVRDQMPIV
LDKQQRIIAVGDLYQQQTIKKWIIISKNGDE
>Mature_431_residues
MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTCLHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDL
SHSLDRNNSIQNEARIKRYEWFDEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQWHDEQYDILQYSAKQFIQEFVKF
DEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYELFNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAK
NNGDTYFRVQTIKKPGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQWVRDQMPIV
LDKQQRIIAVGDLYQQQTIKKWIIISKNGDE

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=192, Percent_Identity=32.2916666666667, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_STAAC (Q5HIG6)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_185441.1
- ProteinModelPortal:   Q5HIG6
- SMR:   Q5HIG6
- STRING:   Q5HIG6
- EnsemblBacteria:   EBSTAT00000009655
- GeneID:   3237121
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL0553
- TIGR:   SACOL0553
- eggNOG:   COG0037
- GeneTree:   EBGT00050000024217
- HOGENOM:   HBG693908
- OMA:   NDISYPL
- ProtClustDB:   CLSK884700
- BioCyc:   SAUR93062:SACOL0553-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 51292; Mature: 51292

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTCLHVNHGVRSASIEEAR
CCCCCCCCEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHH
FLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWFDEMMNVLEADVLLTAHHL
HHHHHHHCCCCEEEEEEECCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLAVSKKEIKQFQERYHIPYFED
HHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQWHDEQYDILQYSAKQFIQEFVKF
CCCCCHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYELFNISAKTYEEWFKQFSSKKAQFSI
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEE
NLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKKPGNYIFNKYRLEIHSNLPKCLFPLTV
ECCCEEEEEEEECEEEEEEECCCCEEEEEEEECCCCCCEEEEEEEEECCCCCHHHCCEEE
RTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQWVRDQMPIVLDKQQRIIAVGDLYQQQTIK
EECCCCCEEEECCCCHHHHHHEEEEEECCCHHHHHCCCEEECCCCCEEEEHHHHHHHHHE
KWIIISKNGDE
EEEEEECCCCC
>Mature Secondary Structure
MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTCLHVNHGVRSASIEEAR
CCCCCCCCEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHH
FLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWFDEMMNVLEADVLLTAHHL
HHHHHHHCCCCEEEEEEECCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLAVSKKEIKQFQERYHIPYFED
HHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQWHDEQYDILQYSAKQFIQEFVKF
CCCCCHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYELFNISAKTYEEWFKQFSSKKAQFSI
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEE
NLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKKPGNYIFNKYRLEIHSNLPKCLFPLTV
ECCCEEEEEEEECEEEEEEECCCCEEEEEEEECCCCCCEEEEEEEEECCCCCHHHCCEEE
RTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQWVRDQMPIVLDKQQRIIAVGDLYQQQTIK
EECCCCCEEEECCCCHHHHHHEEEEEECCCHHHHHCCCEEECCCCCEEEEHHHHHHHHHE
KWIIISKNGDE
EEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA