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The map label for this gene is ksgA

Identifier: 57651375

GI number: 57651375

Start: 545535

End: 546428

Strand: Direct

Name: ksgA

Synonym: SACOL0536

Alternate gene names: 57651375

Gene position: 545535-546428 (Clockwise)

Preceding gene: 57651374

Following gene: 57651376

Centisome position: 19.42

GC content: 32.89

Gene sequence:

>894_bases
ATGTTGGATAATAAAGATATTGCAACACCATCAAGAACGCGAGCGTTGTTAGATAAATATGGCTTTAATTTTAAAAAAAG
TTTAGGACAGAACTTTTTGATAGATGTGAATATCATTAATAATATCATTGATGCAAGTGATATTGATGCACAAACTGGGG
TGATTGAAATTGGTCCAGGCATGGGGTCATTGACAGAACAATTGGCCAGACATGCTAAAAGAGTATTGGCATTTGAAATT
GATCAACGTTTAATACCTGTATTAAATGATACACTATCACCTTATGATAATGTGACGGTGATTAATGAAGATATTTTAAA
AGCGAATATTAAAGAAGCTGTTGAAAATCATTTACAAGATTGTGAAAAAATAATGGTTGTTGCAAACCTGCCGTACTATA
TTACGACGCCAATTTTATTAAATTTGATGCAACAAGATATACCAATTGATGGCTACGTGGTGATGATGCAAAAAGAAGTG
GGCGAACGCTTAAATGCTGAAGTAGGTTCAAAAGCATATGGTTCGTTATCAATTGTCGTACAATACTATACAGAGACTAG
TAAAGTATTAACGGTACCTAAATCTGTATTTATGCCACCACCTAATGTTGATTCAATAGTTGTAAAACTGATGCAGAGAA
CTGAACCGTTAGTAACAGTAGATAACGAGGAAGCATTCTTTAAGTTAGCAAAAGCAGCATTTGCACAAAGAAGAAAGACA
ATTAACAATAACTATCAAAATTATTTTAAAGATGGTAAACAACACAAAGAAGTGATTTTACAATGGTTGGAACAAGCAGG
TATTGATCCAAGACGTCGCGGTGAAACGCTATCTATTCAAGATTTTGCTAAATTGTATGAAGAAAAGAAAAAATTCCCTC
AATTAGAAAATTAA

Upstream 100 bases:

>100_bases
GGCCATTCCAATGGTAAGCAGTTATTGAAAAAGTTAAATGCATTTGGTTATACCGAAGCGGATGTAAGGCAAGCTTTAGA
AGATGAATGAGGAAGTGAAA

Downstream 100 bases:

>100_bases
ATGATTGACAAAGCAAAGCACTATTGTTAAAATTTAAATTTTGTTTGACGAAAACGTTGCAAATATGGTATTATGTAACT
TGTAGCGAGGTGGAGCAATA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIIDASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEI
DQRLIPVLNDTLSPYDNVTVINEDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLVTVDNEEAFFKLAKAAFAQRRKT
INNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGETLSIQDFAKLYEEKKKFPQLEN

Sequences:

>Translated_297_residues
MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIIDASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEI
DQRLIPVLNDTLSPYDNVTVINEDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLVTVDNEEAFFKLAKAAFAQRRKT
INNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGETLSIQDFAKLYEEKKKFPQLEN
>Mature_297_residues
MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIIDASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEI
DQRLIPVLNDTLSPYDNVTVINEDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLVTVDNEEAFFKLAKAAFAQRRKT
INNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGETLSIQDFAKLYEEKKKFPQLEN

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=294, Percent_Identity=30.2721088435374, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI7657198, Length=290, Percent_Identity=28.9655172413793, Blast_Score=107, Evalue=9e-24,
Organism=Escherichia coli, GI1786236, Length=261, Percent_Identity=34.8659003831418, Blast_Score=148, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI25141369, Length=297, Percent_Identity=28.6195286195286, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25146882, Length=223, Percent_Identity=33.6322869955157, Blast_Score=104, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6324989, Length=229, Percent_Identity=29.6943231441048, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21357273, Length=292, Percent_Identity=29.7945205479452, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21358017, Length=298, Percent_Identity=29.1946308724832, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_STAA1 (A7WYP0)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441080.1
- ProteinModelPortal:   A7WYP0
- SMR:   A7WYP0
- STRING:   A7WYP0
- EnsemblBacteria:   EBSTAT00000003081
- GeneID:   5559812
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_0490
- eggNOG:   COG0030
- GeneTree:   EBGT00050000024854
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- ProtClustDB:   PRK00274
- BioCyc:   SAUR418127:SAHV_0490-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 33738; Mature: 33738

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 31-31 BINDING 33-33 BINDING 58-58 BINDING 79-79 BINDING 104-104 BINDING 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIIDASDIDAQTGVIEIGPG
CCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCEEEECCC
MGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVINEDILKANIKEAVENHLQD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHH
CEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEVGERLNAEVGSKAYGSLSIVV
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCCEEEE
QYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLVTVDNEEAFFKLAKAAFAQRRKT
EEHHCCCCEEECCHHHCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHH
INNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGETLSIQDFAKLYEEKKKFPQLEN
HCCCHHHHHHCCHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIIDASDIDAQTGVIEIGPG
CCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCEEEECCC
MGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVINEDILKANIKEAVENHLQD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHH
CEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEVGERLNAEVGSKAYGSLSIVV
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCCEEEE
QYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLVTVDNEEAFFKLAKAAFAQRRKT
EEHHCCCCEEECCHHHCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHH
INNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGETLSIQDFAKLYEEKKKFPQLEN
HCCCHHHHHHCCHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA