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The map label for this gene is phnD [H]

Identifier: 57651149

GI number: 57651149

Start: 143021

End: 143977

Strand: Reverse

Name: phnD [H]

Synonym: SACOL0128

Alternate gene names: 57651149

Gene position: 143977-143021 (Counterclockwise)

Preceding gene: 57651169

Following gene: 57651148

Centisome position: 5.12

GC content: 31.03

Gene sequence:

>957_bases
ATGAAAAATTTTAAGTGTTTATTTGTATTAATGTTAGCAGTCATTGTTTTTGCAGCAGCATGTGGAAACTCAAGTTCTTT
AGATAATCAAAAGAACGCTAGTAATGATTCGGATTCTAAATCAGGAGGATACAAACCTAAAGAATTAACCGTTCAATTTG
TACCTTCGCAAAATGCTGGAACATTAGAAGCTAAAGCAAAACCATTAGAAAAATTACTATCTAAAGAATTAGGGATTCCA
GTTAAAGTGTCTGTATCAACTAACTACAATACAATTGTTGAAGCTATGAAGTCTAAAAAAGTTGATGTTGGTTTCTTACC
ACCAACGGCATACACATTAGCACATGATCAAAAAGCAGCTGATTTATTATTACAAGCACAACGTTTCGGTGTAAAAGAAG
ATGGTTCAGCAAGTAAAGAACTTGTAGATAGTTATAAATCAGAAATTCTTGTTAAAAAAGACTCAAAAATTAAAAGCTTG
AAAGATTTAAAAGGTAAGAAAATTGCCTTACAAGATGTAACATCAACTGCTGGATATACATTCCCACTTGCGATGTTAAA
AAACGAAGCAGGTATTAATGCAACTAAAGATATGAAAATTGTGAATGTTAAAGGTCATGACCAAGCAGTTATCTCATTAT
TAAATGGAGATGTAGATGCTGCGGCTGTATTTAACGATGCACGTAATACTGTGAAAAAAGACCAACCAAATGTATTTAAA
GACACACGAATTTTAAAATTAACACAAGCTATTCCGAATGACACAATTTCTGTAAGACCAGATATGGATAAAGATTTTCA
AGAAAAATTGAAAAAAGCTTTTATAGACATTGCTAAATCAAAAGAAGGTCACAAAATTATTAGCGAAGTTTATTCACATG
AAGGATACACAGAAACGAAAGATTCAAATTTCGACATTGTAAGAGAGTACGAAAAATTAGTTAAAGATATGAAATAA

Upstream 100 bases:

>100_bases
TTTATAAAATACTTTTTAACATTTAAATGTGGTACGCTATAAGTGTAATTTCATTGCATACATATTACACGATTAAGAAT
GTGAAGGGGACAGTTATCAA

Downstream 100 bases:

>100_bases
TCATTATTTAACAAATGAATCATTAGCGAATTTGGTATTAAAAGCTTTCGTTCAATAGATATATTCTAGATTAATATTGA
AAAGCTAGGCGCTAAACTGA

Product: phosphonate ABC transporter phosphonate-binding protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGYKPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIP
VKVSVSTNYNTIVEAMKSKKVDVGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDVDAAAVFNDARNTVKKDQPNVFK
DTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFIDIAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK

Sequences:

>Translated_318_residues
MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGYKPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIP
VKVSVSTNYNTIVEAMKSKKVDVGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDVDAAAVFNDARNTVKKDQPNVFK
DTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFIDIAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK
>Mature_318_residues
MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGYKPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIP
VKVSVSTNYNTIVEAMKSKKVDVGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDVDAAAVFNDARNTVKKDQPNVFK
DTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFIDIAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK

Specific function: Part of the ABC transporter complex phnCDE involved in phosphate import. Responsible for phosphate binding [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790543, Length=276, Percent_Identity=24.2753623188406, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015168
- InterPro:   IPR005770 [H]

Pfam domain/function: PF09084 NMT1 [H]

EC number: NA

Molecular weight: Translated: 35001; Mature: 35001

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGYKPKELTVQFVPSQNAG
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
TLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVDVGFLPPTAYTLAHDQKAA
CCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCEECCCCCHHHHHCCHHHH
DLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSLKDLKGKKIALQDVTSTAGYT
HHHHHHHHCCCCCCCCHHHHHHHHHHHHEEEECCCHHHHHHHCCCCEEEEEHHHCCCCCH
FPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDVDAAAVFNDARNTVKKDQPNVFK
HHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHH
DTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFIDIAKSKEGHKIISEVYSHEGYTETK
HHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC
DSNFDIVREYEKLVKDMK
CCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGYKPKELTVQFVPSQNAG
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
TLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVDVGFLPPTAYTLAHDQKAA
CCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCEECCCCCHHHHHCCHHHH
DLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSLKDLKGKKIALQDVTSTAGYT
HHHHHHHHCCCCCCCCHHHHHHHHHHHHEEEECCCHHHHHHHCCCCEEEEEHHHCCCCCH
FPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDVDAAAVFNDARNTVKKDQPNVFK
HHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHH
DTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFIDIAKSKEGHKIISEVYSHEGYTETK
HHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC
DSNFDIVREYEKLVKDMK
CCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA