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The map label for this gene is hsdR [H]

Identifier: 57650998

GI number: 57650998

Start: 2551139

End: 2554000

Strand: Reverse

Name: hsdR [H]

Synonym: SACOL2499

Alternate gene names: 57650998

Gene position: 2554000-2551139 (Counterclockwise)

Preceding gene: 57650999

Following gene: 57650997

Centisome position: 90.91

GC content: 28.06

Gene sequence:

>2862_bases
ATGAGTAGATTACTAAATGATTTCAATCAATCTTTACATAAAGGGTTTATAGACAAACATATTTCTCATAAAGGGAATTA
TACACCGAAGCTTTTAGTAAATAATAAAAATGAAAAAGTTCTTTCAACAATTATAGATGAACTTCAAAAATGTGAAACGT
TTTATTTTTCTGTGGCTTTTATAACAGAAAGCGGTTTAGCCAGCTTAAAGGCTCAACTATTAGATTTAAGCAATAAGGGT
GTTAAAGGGAAAATATTAACATCTAATTACTTAGGGTTTAATAGTCCTAAAATGTATGGAGAATTACTTAAATTAAAAAA
TGTAGAGGTAAGATTAACTGATATTGCTGGATTCCATGCCAAAGGATATATTTTTGAGCATAAGGATTATAGTTCTATGG
TAATAGGAAGCTCTAATTTAACATCTAATGCGTTAAAGGTTAATTATGAGCATAATGTTTTACTGTCTACTATGAAAAAT
GGCGATTTAGTTGATAGTGTAAAAAGTGAATTTGACTTGTTATGGCAAAAGAGTACCCCACTGACTGAGCAATGGATTAA
TTCATATAAAGAATCATTTGAGTACCGTTCCTTAGAAAAATTAGCGGAGGTTGAACAAACTCAAATGCTGCTAGCTGATA
AGGTGAAAAAATCAGTGGAAATTGTACCCAATTTAATGCAAGCAGAAGCATTAAGGTCATTAAAAGCTATTAGGGATAAA
GCCAAAGATAAGGCGTTAATAATATCTGCAACTGGTACAGGTAAAACGATTTTATGTGCATTAGATGTTAGAGAAGTAAA
CCCTAATAAATTTTTATTTATTGTTCATAATGAGGGGATTTTAAATAGAGCTAAGGAAGAATTTAAAAAAGTATTACCTA
TAAAAAATGATAGTGATTTTGGATTGTTGACAGGAAAACATAGAGATGTCGATGCCAAATATTTATTTGCAACCATTCAA
ACATTATCTAGAGATGATAATTTTAAGCAATTTGATGAAAATGAATTTGATTATATTGTTTTTGATGAAGCACATCGTTC
TGCTGCATCTACTTATCAACGAGTATTTAACTACTTCAAACCTAAGTTCATGCTGGGAATGACTGCTACACCAGAAAGAT
CAGATGAATTAAGTATATTTGAACTGTTTGATTATAATATTGCATATGAAATAAGGTTACAAGCAGCATTAGAAAGTGAT
ATTTTATGTCCATTTCATTATTTTGGTGTGACTGATTATGTACATCAAGGTATTAAAGAAGATGATGTAACTAAACTGAG
ATATTTAACTTCTGATGAAAGAGTTAATTATATTATTCAAAAGACAGATTACTATGGATATTCAGGTGAAATTTTACAAG
GATTGATTTTTGTAAGTAGCAAAAAAGAAGCTTATGATTTAGCGGATAAACTAAGTTCAAAAGGGATTAAAAGTGTTGCT
TTAACAGGAGATGATAGTGTTAATTATCGCCAAATTGTAATAGAAAAATTAAAAGAAGGTAAGATTAACTATATTATAAC
TGTAGATCTTTTTAACGAAGGTATTGATATTCCAGAAGTCAATCAAGTTGTAATGTTGAGACCAACAGAGTCTAGTATTA
TATTCATTCAGCAACTTGGTAGAGGATTAAGAAAAAGTTCAAATAAAGAGTATGTTACAGTAATTGATTTTATTGGTAAT
TATAAGACAAATTATTTAATTCCGATTGCGCTTTCTGGGGATCAATCGCAAAATAAAGATAATTATAAGAAGTTTTTAAC
GAATAACGATTCGATTAATGGAGTATCTACAATTAATTTTGAAGAAGTTGCTAAAAAACAGATTTACAATTCATTGGATG
CAGTATCATTAAATCAAAATAAATTAATATTAAAAGCTTATGAAGAAGTTGAAAATAGATTGGGACACATGCCGTTACTA
ATGGATTTCATACAACAACATTCTATAGATCCAAGCGTTATATTTTCTAAATTTAGTAATTATTACGAGTTCTTAGTGAG
ATATAAAAAAATAGATACATTATTGACGGAAAATGAATCTAAAAATCTGGTTTTCTTTTCAAGGCAAATAGCACCTGGAT
TGAAAAGAATCGATAGTTTAGTTTTAGAAGAACTTTTAAAAAATGAATTAACATATGATGAATTAAAAAATAAAATGTTG
AACGAAGTTAAGGATATAACAGAAGATGATATAGATACTTCATTAAGAATTTTAGATTTTTCATTTTACAACGCAGGAAT
TGAAAAAATCTATGGAAGTCCCATTATAGAACGTAATGAAAGAATGATAAGACTAAGCGATGCATTTACTAATGCTCTAA
GTAATCAAACATTTAATATGTTTTTAGAAGATCTTATTGAATTATCAAAGTATAACAATGAAAAATACCAAAAAGGTAAA
AATGGACTGATTTTATATAATAAATATTCAAGAGAAGATTTTAGTAAAATATTTAATTGGAATAAAAATGGTTCGTCTGT
AATCATGGGATACATGATAAAAAGCCAAGAAATGCCAATATTTATCACTTATGATAAGCATGAGGATATAAGTGATAGCA
CAAAATATGAAGATGAATTTTTAAGTCAAGATGAATTAAAATGGTTTACTAAATCTAATAGAACGCTAGAATCAAAAGAA
GTTCAGAAAATTTTGTCCCATAGAGCAAAAGGAATTAAAATGTATATTTTTGTACAGAAAAAAGATGATGATGGTATATA
TTTTTATTATTTAGGAACTGCTGGATATATAGAAGGTTCAGAAAAGCAAGATAAAATGCCGAATGGTTCAAACGTAGTAA
CGATGGATTTAGCACTGGATAAAGCTGTAAGAGATGATATATATCGTTATCTAACAAATTAA

Upstream 100 bases:

>100_bases
CATAAGAGTATTGAATGGTTGTCAATAAACGAACTTGATAAATTAAATTGGGCCCCAGCGGATATACCAGCCGTTAATAA
AATTATGACTGAGGGATAAT

Downstream 100 bases:

>100_bases
TGTAAACCGAAAAATGAATGTTAGTAAAGTTGTATTTAAACATCAATTTTAATAAGTAAATACATTGAGTTTCAGTGTTA
TTATTTTAAATATAAGCGCA

Product: helicase

Products: NA

Alternate protein names: R.EcoEI [H]

Number of amino acids: Translated: 953; Mature: 952

Protein sequence:

>953_residues
MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTIIDELQKCETFYFSVAFITESGLASLKAQLLDLSNKG
VKGKILTSNYLGFNSPKMYGELLKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
GDLVDSVKSEFDLLWQKSTPLTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKSVEIVPNLMQAEALRSLKAIRDK
AKDKALIISATGTGKTILCALDVREVNPNKFLFIVHNEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQ
TLSRDDNFKQFDENEFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIRLQAALESD
ILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGEILQGLIFVSSKKEAYDLADKLSSKGIKSVA
LTGDDSVNYRQIVIEKLKEGKINYIITVDLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGN
YKTNYLIPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLILKAYEEVENRLGHMPLL
MDFIQQHSIDPSVIFSKFSNYYEFLVRYKKIDTLLTENESKNLVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKML
NEVKDITEDDIDTSLRILDFSFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFNMFLEDLIELSKYNNEKYQKGK
NGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIKSQEMPIFITYDKHEDISDSTKYEDEFLSQDELKWFTKSNRTLESKE
VQKILSHRAKGIKMYIFVQKKDDDGIYFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYLTN

Sequences:

>Translated_953_residues
MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTIIDELQKCETFYFSVAFITESGLASLKAQLLDLSNKG
VKGKILTSNYLGFNSPKMYGELLKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
GDLVDSVKSEFDLLWQKSTPLTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKSVEIVPNLMQAEALRSLKAIRDK
AKDKALIISATGTGKTILCALDVREVNPNKFLFIVHNEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQ
TLSRDDNFKQFDENEFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIRLQAALESD
ILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGEILQGLIFVSSKKEAYDLADKLSSKGIKSVA
LTGDDSVNYRQIVIEKLKEGKINYIITVDLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGN
YKTNYLIPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLILKAYEEVENRLGHMPLL
MDFIQQHSIDPSVIFSKFSNYYEFLVRYKKIDTLLTENESKNLVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKML
NEVKDITEDDIDTSLRILDFSFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFNMFLEDLIELSKYNNEKYQKGK
NGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIKSQEMPIFITYDKHEDISDSTKYEDEFLSQDELKWFTKSNRTLESKE
VQKILSHRAKGIKMYIFVQKKDDDGIYFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYLTN
>Mature_952_residues
SRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTIIDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGV
KGKILTSNYLGFNSPKMYGELLKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKNG
DLVDSVKSEFDLLWQKSTPLTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKSVEIVPNLMQAEALRSLKAIRDKA
KDKALIISATGTGKTILCALDVREVNPNKFLFIVHNEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQT
LSRDDNFKQFDENEFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIRLQAALESDI
LCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGEILQGLIFVSSKKEAYDLADKLSSKGIKSVAL
TGDDSVNYRQIVIEKLKEGKINYIITVDLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNY
KTNYLIPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLILKAYEEVENRLGHMPLLM
DFIQQHSIDPSVIFSKFSNYYEFLVRYKKIDTLLTENESKNLVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKMLN
EVKDITEDDIDTSLRILDFSFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFNMFLEDLIELSKYNNEKYQKGKN
GLILYNKYSREDFSKIFNWNKNGSSVIMGYMIKSQEMPIFITYDKHEDISDSTKYEDEFLSQDELKWFTKSNRTLESKEV
QKILSHRAKGIKMYIFVQKKDDDGIYFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYLTN

Specific function: The EcoEI enzyme recognizes 5'-GAGN(7)ATGC-3'. Subunit R is required for both nuclease and ATPase activities, but not for modification [H]

COG id: COG1061

COG function: function code KL; DNA or RNA helicases of superfamily II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788511, Length=377, Percent_Identity=22.8116710875332, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320539, Length=381, Percent_Identity=26.7716535433071, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR013670
- InterPro:   IPR007409 [H]

Pfam domain/function: PF08463 EcoEI_R_C; PF04313 HSDR_N; PF04851 ResIII [H]

EC number: =3.1.21.3 [H]

Molecular weight: Translated: 109902; Mature: 109771

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTIIDELQKCETFYFSVAF
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHEEEEEE
ITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGELLKLKNVEVRLTDIAGFHA
EECCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEECCCCC
KGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKNGDLVDSVKSEFDLLWQKSTP
CCEEEECCCCCEEEEECCCCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHHCCCCCC
LTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKSVEIVPNLMQAEALRSLKAIRDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKDKALIISATGTGKTILCALDVREVNPNKFLFIVHNEGILNRAKEEFKKVLPIKNDSDF
CCCCEEEEEECCCCCEEEEEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCC
GLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDENEFDYIVFDEAHRSAASTYQRVFNYFK
EEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCC
PKFMLGMTATPERSDELSIFELFDYNIAYEIRLQAALESDILCPFHYFGVTDYVHQGIKE
CEEEEEECCCCCCCCCCEEEEEECCCEEEEEEEEEHHCCCCCCCHHHHCHHHHHHCCCCC
DDVTKLRYLTSDERVNYIIQKTDYYGYSGEILQGLIFVSSKKEAYDLADKLSSKGIKSVA
CCCHHHHEECCCCCEEEEEEECCCCCCCHHHHHEEHEECCCCHHHHHHHHHHCCCCCEEE
LTGDDSVNYRQIVIEKLKEGKINYIITVDLFNEGIDIPEVNQVVMLRPTESSIIFIQQLG
EECCCCCCHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCEEEEECCCCCEEEHHHHH
RGLRKSSNKEYVTVIDFIGNYKTNYLIPIALSGDQSQNKDNYKKFLTNNDSINGVSTINF
HHHHCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCEEECCH
EEVAKKQIYNSLDAVSLNQNKLILKAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSN
HHHHHHHHHHCCCCEECCCCEEHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHH
YYEFLVRYKKIDTLLTENESKNLVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKML
HHHHHHHHHHHHHHHCCCCCCCEEEEEHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NEVKDITEDDIDTSLRILDFSFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFNM
HHHHHCCCCCCCCEEEEEEEHHHHHHHHHHCCCCCEECCCCEEEEHHHHHHHHCCCHHHH
FLEDLIELSKYNNEKYQKGKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIKSQEMPI
HHHHHHHHHHCCCHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCCEEEEEEEEECCCCCE
FITYDKHEDISDSTKYEDEFLSQDELKWFTKSNRTLESKEVQKILSHRAKGIKMYIFVQK
EEEECCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEEEE
KDDDGIYFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYLTN
ECCCCEEEEEECCCEECCCCCHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTIIDELQKCETFYFSVAF
CHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHEEEEEE
ITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGELLKLKNVEVRLTDIAGFHA
EECCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEECCCCC
KGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKNGDLVDSVKSEFDLLWQKSTP
CCEEEECCCCCEEEEECCCCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHHCCCCCC
LTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKSVEIVPNLMQAEALRSLKAIRDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKDKALIISATGTGKTILCALDVREVNPNKFLFIVHNEGILNRAKEEFKKVLPIKNDSDF
CCCCEEEEEECCCCCEEEEEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCC
GLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDENEFDYIVFDEAHRSAASTYQRVFNYFK
EEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCC
PKFMLGMTATPERSDELSIFELFDYNIAYEIRLQAALESDILCPFHYFGVTDYVHQGIKE
CEEEEEECCCCCCCCCCEEEEEECCCEEEEEEEEEHHCCCCCCCHHHHCHHHHHHCCCCC
DDVTKLRYLTSDERVNYIIQKTDYYGYSGEILQGLIFVSSKKEAYDLADKLSSKGIKSVA
CCCHHHHEECCCCCEEEEEEECCCCCCCHHHHHEEHEECCCCHHHHHHHHHHCCCCCEEE
LTGDDSVNYRQIVIEKLKEGKINYIITVDLFNEGIDIPEVNQVVMLRPTESSIIFIQQLG
EECCCCCCHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCEEEEECCCCCEEEHHHHH
RGLRKSSNKEYVTVIDFIGNYKTNYLIPIALSGDQSQNKDNYKKFLTNNDSINGVSTINF
HHHHCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCEEECCH
EEVAKKQIYNSLDAVSLNQNKLILKAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSN
HHHHHHHHHHCCCCEECCCCEEHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHH
YYEFLVRYKKIDTLLTENESKNLVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKML
HHHHHHHHHHHHHHHCCCCCCCEEEEEHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NEVKDITEDDIDTSLRILDFSFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFNM
HHHHHCCCCCCCCEEEEEEEHHHHHHHHHHCCCCCEECCCCEEEEHHHHHHHHCCCHHHH
FLEDLIELSKYNNEKYQKGKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIKSQEMPI
HHHHHHHHHHCCCHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCCEEEEEEEEECCCCCE
FITYDKHEDISDSTKYEDEFLSQDELKWFTKSNRTLESKEVQKILSHRAKGIKMYIFVQK
EEEECCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEEEE
KDDDGIYFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYLTN
ECCCCEEEEEECCCEECCCCCHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]