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The map label for this gene is bioA [H]

Identifier: 57650971

GI number: 57650971

Start: 2485322

End: 2486680

Strand: Reverse

Name: bioA [H]

Synonym: SACOL2427

Alternate gene names: 57650971

Gene position: 2486680-2485322 (Counterclockwise)

Preceding gene: 57650972

Following gene: 57650970

Centisome position: 88.51

GC content: 33.7

Gene sequence:

>1359_bases
ATGAATTACACACAACAACTTAAACAAAAAGACTCAGAATATGTTTGGCATCCATTTACACAAATGGGTGTATATAGCAA
AGAAGAAGCAATCATCATTGAAAAAGGAAAGGGTAGTTACCTTTACGATACGAATGGCAATAAATATTTAGATGGTTATG
CATCGTTGTGGGTCAATGTGCATGGTCATAATAACAAATACTTAAATAAGGTAATTAAAAAGCAACTCAATAAAATTGCC
CATTCTACGCTGCTAGGATCATCAAATATTCCGTCAATAGAACTTGCGGAAAAATTAATCGAAATCACGCCAAGTAATCT
AAGAAAAGTATTTTATTCTGATACAGGCAGTGCGTCTGTTGAAATCGCAATAAAGATGGCATATCAGTATTGGAAAAATA
TTGATAGAGAAAAATATGCCAAGAAAAACAAGTTTATAACGCTAAATCACGGTTATCATGGGGATACGATTGGTGCGGTA
AGTGTTGGTGGTATCAAGACCTTTCATAAAATATTTAAAGACTTAATATTTGAGAATATTCAAGTAGAAAGCCCATCTTT
CTATCGCAGTAATTACGATACTGAAAATGAAATGATGACAGCTATTTTAACGAATATAGAGCAAATTCTAATTGAAAGAA
ATGATGAAATCGCAGGGTTTATATTGGAACCGTTGATTCAAGGTGCGACAGGCTTGTTTGTTCATCCTAAAGGCTTTTTG
AAAGAAGTCGAGAAATTGTGCAAAAAATACGATGTCTTATTAATTTGTGATGAGGTAGCAGTTGGTTTTGGGAGAACTGG
AAAGATGTTTGCATGCAATCATGAAGATGTTCAACCGGATATTATGTGTTTAGGTAAGGCGATTACTGGTGGCTACTTAC
CACTTGCAGCTACATTGACATCTAAAAAAATATACAATGCATTTTTAAGTGATTCGCATGGTGTGAATACCTTTTTCCAT
GGTCATACATACACCGGAAATCAAATCGTTTGTACGGTTGCATTAGAAAATATAAGACTTTATGAAAAACGTAAGTTATT
GTCACATATTGAAACGACATCATCAACACTTGAGAAACAGTTACATGCGCTGAAGCGTCATCGAAATGTTGGTGATGTAA
GAGGACGAGGCTTAATGTTTGGAGTTGAATTAGTTACAGATAAAGATAGTAAAACGCCGTTAGAAATTGAAAAAGTTGAA
CGTATTGTACGTAATTGTAAAGAAAACGGGCTAATGATTAGAAATTTAGAAAATGTCATTACGTTTGTGCCAGTGTTAAG
TATGTCAAATAAAGAAGTGAAAACGATGGTACGTATTTTTAAAAAGGCAGTACATAACATTTTAGATAGGAAGTGTTAA

Upstream 100 bases:

>100_bases
ATTGGAAAATTAACAAATAAAACAGTCTATACATTTGAAGAACATGCCACGTATGAAAATTTCTCAGAAGCATTTTTAAA
ACAATTAATAGGAGTTAAAA

Downstream 100 bases:

>100_bases
TATGAATTTGGCTAAACGCATATTACAAGGGGAACAATTAACAAAAGAGACTGTATTGAAAATTTATGAGGATACTAATA
TTGATACCTTAGATTTATTA

Product: adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Products: S-adenosyl-4-methylthio-2-oxobutanoate; 7,8-diaminononanoate

Alternate protein names: 7,8-diamino-pelargonic acid aminotransferase; DAPA aminotransferase [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDTNGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIA
HSTLLGSSNIPSIELAEKLIEITPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIAGFILEPLIQGATGLFVHPKGFL
KEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHEDVQPDIMCLGKAITGGYLPLAATLTSKKIYNAFLSDSHGVNTFFH
GHTYTGNQIVCTVALENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKTPLEIEKVE
RIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDRKC

Sequences:

>Translated_452_residues
MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDTNGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIA
HSTLLGSSNIPSIELAEKLIEITPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIAGFILEPLIQGATGLFVHPKGFL
KEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHEDVQPDIMCLGKAITGGYLPLAATLTSKKIYNAFLSDSHGVNTFFH
GHTYTGNQIVCTVALENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKTPLEIEKVE
RIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDRKC
>Mature_452_residues
MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDTNGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIA
HSTLLGSSNIPSIELAEKLIEITPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIAGFILEPLIQGATGLFVHPKGFL
KEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHEDVQPDIMCLGKAITGGYLPLAATLTSKKIYNAFLSDSHGVNTFFH
GHTYTGNQIVCTVALENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKTPLEIEKVE
RIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDRKC

Specific function: Catalyzes the formation of the intermediate 7,8- diaminopelargonic acid (DAPA) from 7-keto-8-aminopelargonic acid (KAPA) with lysine as the amino donor. B.subtilis does not utilize S-adenosylmethionine (SAM), but instead utilizes only L-lysine as the amin

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily [H]

Homologues:

Organism=Homo sapiens, GI13994255, Length=448, Percent_Identity=28.5714285714286, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI37574042, Length=427, Percent_Identity=29.03981264637, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI4557809, Length=416, Percent_Identity=28.8461538461538, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI226442705, Length=426, Percent_Identity=28.8732394366197, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI24119277, Length=430, Percent_Identity=27.2093023255814, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI226442709, Length=388, Percent_Identity=29.1237113402062, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI284507298, Length=333, Percent_Identity=29.1291291291291, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI188536080, Length=335, Percent_Identity=22.9850746268657, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI38679950, Length=335, Percent_Identity=22.9850746268657, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI38679946, Length=335, Percent_Identity=22.9850746268657, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1786991, Length=437, Percent_Identity=32.4942791762014, Blast_Score=265, Evalue=4e-72,
Organism=Escherichia coli, GI145693181, Length=362, Percent_Identity=32.8729281767956, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1788044, Length=431, Percent_Identity=28.3062645011601, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1789759, Length=429, Percent_Identity=27.5058275058275, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1789016, Length=453, Percent_Identity=26.2693156732892, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1787560, Length=421, Percent_Identity=26.1282660332542, Blast_Score=125, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI32564660, Length=448, Percent_Identity=29.9107142857143, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI25144271, Length=446, Percent_Identity=27.5784753363229, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI25144274, Length=245, Percent_Identity=33.8775510204082, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI71992977, Length=411, Percent_Identity=25.3041362530414, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17541228, Length=388, Percent_Identity=22.680412371134, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324386, Length=464, Percent_Identity=30.3879310344828, Blast_Score=217, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6324432, Length=438, Percent_Identity=29.6803652968037, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6323470, Length=447, Percent_Identity=26.8456375838926, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6321456, Length=446, Percent_Identity=23.3183856502242, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357415, Length=422, Percent_Identity=29.1469194312796, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI161085790, Length=446, Percent_Identity=28.0269058295964, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI28574759, Length=451, Percent_Identity=27.9379157427938, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI21356575, Length=381, Percent_Identity=27.0341207349081, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI281366494, Length=452, Percent_Identity=24.5575221238938, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24667139, Length=452, Percent_Identity=24.5575221238938, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24667143, Length=452, Percent_Identity=24.5575221238938, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.62

Molecular weight: Translated: 51405; Mature: 51405

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDTNGNKYLDGYASLWVNV
CCHHHHHHHCCCCEEECCHHHHCCCCCCCEEEEECCCCCEEEECCCCEEECCEEEEEEEE
HGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEITPSNLRKVFYSDTGSASV
ECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHEEEECCCCCCEE
EIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAVSVGGIKTFHKIFKDLIFENI
EEHHHHHHHHHHCCCHHHHHHCCCEEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHCC
QVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIAGFILEPLIQGATGLFVHPKGFL
CCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCHHHH
KEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHEDVQPDIMCLGKAITGGYLPLAATLT
HHHHHHHHHCCEEEEECHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHH
SKKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVALENIRLYEKRKLLSHIETTSSTLEKQ
HHHHHHHHHCCCCCCCEEECCEEECCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
LHALKRHRNVGDVRGRGLMFGVELVTDKDSKTPLEIEKVERIVRNCKENGLMIRNLENVI
HHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEECHHHHH
TFVPVLSMSNKEVKTMVRIFKKAVHNILDRKC
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDTNGNKYLDGYASLWVNV
CCHHHHHHHCCCCEEECCHHHHCCCCCCCEEEEECCCCCEEEECCCCEEECCEEEEEEEE
HGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEITPSNLRKVFYSDTGSASV
ECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHEEEECCCCCCEE
EIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAVSVGGIKTFHKIFKDLIFENI
EEHHHHHHHHHHCCCHHHHHHCCCEEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHCC
QVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIAGFILEPLIQGATGLFVHPKGFL
CCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCHHHH
KEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHEDVQPDIMCLGKAITGGYLPLAATLT
HHHHHHHHHCCEEEEECHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHH
SKKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVALENIRLYEKRKLLSHIETTSSTLEKQ
HHHHHHHHHCCCCCCCEEECCEEECCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
LHALKRHRNVGDVRGRGLMFGVELVTDKDSKTPLEIEKVERIVRNCKENGLMIRNLENVI
HHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEECHHHHH
TFVPVLSMSNKEVKTMVRIFKKAVHNILDRKC
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; 8-amino-7-oxononanoate

Specific reaction: S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8763940; 9387221; 9384377 [H]