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The map label for this gene is narH [H]

Identifier: 57650939

GI number: 57650939

Start: 2451676

End: 2453235

Strand: Reverse

Name: narH [H]

Synonym: SACOL2394

Alternate gene names: 57650939

Gene position: 2453235-2451676 (Counterclockwise)

Preceding gene: 57650940

Following gene: 57650938

Centisome position: 87.32

GC content: 38.08

Gene sequence:

>1560_bases
TTGAAGATTAAAGCGCAAGTTGCGATGGTATTAAATTTAGATAAATGCATAGGATGCCATACGTGTAGTGTGACATGTAA
AAACACTTGGACAAATCGTCCAGGTGCTGAGTACATGTGGTTCAACAACGTAGAAACGAAGCCAGGTGTAGGGTATCCGA
AACGTTGGGAAGACCAAGAACACTACAAAGGTGGTTGGGTACTAAATCGTAAAGGGAAACTTGAATTAAAATCTGGAAGT
AGAATTTCAAAAATTGCTTTAGGTAAAATTTTTTATAACCCAGATATGCCATTAATTAAAGATTATTATGAGCCATGGAA
CTATAATTATGAACATTTAACAACTGCGAAATCAGGGAAGCATTCGCCAGTTGCTAGAGCGTATTCAGAAATTACGGGGG
ATAACATTGAAATTGAATGGGGACCTAACTGGGAAGATGACTTAGCAGGTGGTCATGTTACAGGTCCAAAAGATCCTAAC
ATCCAAAAAATAGAAGAAGATATTAAATTCCAATTTGACGAAACTTTTATGATGTATTTACCACGTTTATGTGAACATTG
CTTGAATCCAAGTTGCGTTGCATCGTGTCCTTCAGGTGCTATGTATAAACGTGATGAAGATGGCATTGTATTAGTTGACC
AAGACGCATGTCGTGGATGGCGTTACTGTATGACAGGTTGTCCATATAAGAAAGTTTACTTCAATTGGAAAACGAACAAA
GCTGAAAAGTGTACTTTCTGTTTCCCGAGAATCGAGGCGGGTATGCCTACGGTATGCTCTGAAACTTGTACAGGACGTAT
GCGTTACTTAGGTGTTTTATTATATGATGCAGATCGAGTTCATGAAGCAGCTTCAGCAGTAGACGAGAAAGATTTATATG
AAAAACAATTAGATATATTCTTAAACCCATTTGACGAAGAAGTCATTGCTCAAGCTGAAAAAGATGGTATTGGTTACGAT
TGGATTGAAGCTGCACAAAACTCTCCAATTTATAAGTTAGCAATTGAATATAAACTTGCATTTCCGTTACATCCTGAATT
TAGAACGATGCCGATGGTTTGGTATTGCCCACCACTTAGCCCAATTATGAGCTATTTTGAAGGTAAAAATACAACTCAAA
ATCCAGATGCGATTTTCCCAGCCATTGAAGAAATGCGTTTACCTATTGAATACTTAGCTAATATTTTCACTGCAGGAGAT
ACAGAGCCAGTAAAAGGAGCCCTACAACGTATGGCAATGATGAGAAGTTATATGAGATCTCAAGTTACTCAACAGCCATT
TGACACGTCTCGATTAGAGCGATTAGGTATAACCGAGCGCCAAACTAAAGACATGTATCGTTTATTAGGACTAGCCAAAT
ATGAAGATCGATTTGTTATTCCAACATCACACAAAGAAACCTATTTAGATACGTATCACGCACAAGGTAGTACAGGATAC
AATTACGGCGGCGAGCATTTTGGAGATAACTGTGAAGGCTGTGGCGTTGCAGTAGGTTCAGGGAAAACTGGTCAAGAAAT
TTATAATGAGAATTTCTATGGAGGGATTTTCCGTGATTAA

Upstream 100 bases:

>100_bases
GGATACGCACAAATTAGTTATCACTTTAATTATTATGGACCAATTGGGAACCAAAGGGATTTATATGTAGCAGTTAGAAA
GATGAAGGAGGTTAATTGGC

Downstream 100 bases:

>100_bases
TTTCGATAATTTTAAAAAATATCAAGAAAGTTTTGGATATATGGCACAACAGTTATGTTTTCCAGAAAAATTAACTTTTC
ATCCAAAAACTTTTGAAGAA

Product: respiratory nitrate reductase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNVETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGS
RISKIALGKIFYNPDMPLIKDYYEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNK
AEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYDADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYD
WIEAAQNSPIYKLAIEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYLANIFTAGD
TEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLGLAKYEDRFVIPTSHKETYLDTYHAQGSTGY
NYGGEHFGDNCEGCGVAVGSGKTGQEIYNENFYGGIFRD

Sequences:

>Translated_519_residues
MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNVETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGS
RISKIALGKIFYNPDMPLIKDYYEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNK
AEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYDADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYD
WIEAAQNSPIYKLAIEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYLANIFTAGD
TEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLGLAKYEDRFVIPTSHKETYLDTYHAQGSTGY
NYGGEHFGDNCEGCGVAVGSGKTGQEIYNENFYGGIFRD
>Mature_519_residues
MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNVETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGS
RISKIALGKIFYNPDMPLIKDYYEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNK
AEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYDADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYD
WIEAAQNSPIYKLAIEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYLANIFTAGD
TEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLGLAKYEDRFVIPTSHKETYLDTYHAQGSTGY
NYGGEHFGDNCEGCGVAVGSGKTGQEIYNENFYGGIFRD

Specific function: The Nitrate Reductase Enzyme Complex Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In The Formation Of Iron-Sulfur Centers. Electrons A

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=495, Percent_Identity=60.2020202020202, Blast_Score=633, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=494, Percent_Identity=58.7044534412955, Blast_Score=627, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=109, Percent_Identity=40.3669724770642, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1787122, Length=109, Percent_Identity=40.3669724770642, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI226510944, Length=126, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI2367345, Length=141, Percent_Identity=29.7872340425532, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789370, Length=97, Percent_Identity=37.1134020618557, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=35.632183908046, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=34.4827586206897, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 59431; Mature: 59431

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNVETKPGVGYPKRWEDQE
CCCCEEEEEEEEHHHHCCCEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHH
HYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDYYEPWNYNYEHLTTAKSGK
HCCCCEEECCCCCEEECCCCCEEHEEEEEEEECCCCCHHHHHCCCCCCCHHEEEECCCCC
HSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPNIQKIEEDIKFQFDETFMMYL
CCHHHHHHHHHCCCEEEEEECCCCCHHCCCCCCCCCCCCCHHHHHHHHHEEECHHHHHHH
PRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNK
HHHHHHHCCCHHHHCCCCCCCEEECCCCEEEECCHHHCCHHHHHCCCCCEEEEEEECCCC
AEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYDADRVHEAASAVDEKDLYEKQLDIF
CCEEEEECCHHCCCCCHHHHHHHCCHHHHEEEEEECHHHHHHHHHHCCHHHHHHHHHHHE
LNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLAIEYKLAFPLHPEFRTMPMVWYCPPLS
ECCCCHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCEEEECCCHH
PIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYLANIFTAGDTEPVKGALQRMAMMRSYMRS
HHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
QVTQQPFDTSRLERLGITERQTKDMYRLLGLAKYEDRFVIPTSHKETYLDTYHAQGSTGY
HHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCCCC
NYGGEHFGDNCEGCGVAVGSGKTGQEIYNENFYGGIFRD
CCCCHHCCCCCCCCEEEECCCCCCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNVETKPGVGYPKRWEDQE
CCCCEEEEEEEEHHHHCCCEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHH
HYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDYYEPWNYNYEHLTTAKSGK
HCCCCEEECCCCCEEECCCCCEEHEEEEEEEECCCCCHHHHHCCCCCCCHHEEEECCCCC
HSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPNIQKIEEDIKFQFDETFMMYL
CCHHHHHHHHHCCCEEEEEECCCCCHHCCCCCCCCCCCCCHHHHHHHHHEEECHHHHHHH
PRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNK
HHHHHHHCCCHHHHCCCCCCCEEECCCCEEEECCHHHCCHHHHHCCCCCEEEEEEECCCC
AEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYDADRVHEAASAVDEKDLYEKQLDIF
CCEEEEECCHHCCCCCHHHHHHHCCHHHHEEEEEECHHHHHHHHHHCCHHHHHHHHHHHE
LNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLAIEYKLAFPLHPEFRTMPMVWYCPPLS
ECCCCHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCEEEECCCHH
PIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYLANIFTAGDTEPVKGALQRMAMMRSYMRS
HHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
QVTQQPFDTSRLERLGITERQTKDMYRLLGLAKYEDRFVIPTSHKETYLDTYHAQGSTGY
HHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCCCC
NYGGEHFGDNCEGCGVAVGSGKTGQEIYNENFYGGIFRD
CCCCHHCCCCCCCCEEEECCCCCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377 [H]