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The map label for this gene is yhcA [H]

Identifier: 57650895

GI number: 57650895

Start: 2406186

End: 2408117

Strand: Reverse

Name: yhcA [H]

Synonym: SACOL2347

Alternate gene names: 57650895

Gene position: 2408117-2406186 (Counterclockwise)

Preceding gene: 57650896

Following gene: 57650893

Centisome position: 85.72

GC content: 32.82

Gene sequence:

>1932_bases
ATGACTACGACCTTCATTATTAGCTACATTATTTTAGCGCTCATTATTGTTGGGGTTATCAATTTATTTTTAATAAGATC
AAGAAAAAAAGGCAAACGCCAACAAAAGGAACAACAATTTACGACACGTCAATCAAATCAATCTAAATTTAAAGCTAGTG
ATTTAGACAAAACAACTGATCAATCGACACAACGTATGACGCATGAAGAGTTGCGTGTTGACAATCAAGATGATCATAGC
CAAGTTAGTCTAAATGGTTACACAAAGGGGTCTGAGAAAGATCAAGAAGCATTCACTAATAATAAGGATGAGGAAGCAGT
TGCTGCTAAAAATCCTGAATCAGAAGAATATAAAGTGAATGAAAAAATAAAAAAAGAACATAAAAACTTTATTTTTGGTG
AAGGTGTTTCACGCGGTAAAATATTAGCGGCATTATTATTCGGTATGTTTATTGCGATTTTAAACCAAACATTATTAAAT
GTTGCTTTACCAAAAATTAATACGGAATTTAATATTTCTGCGTCAACAGGGCAATGGTTGATGACGGGATTCATGTTAGT
AAACGGTATTTTAATACCAATTACGGCGTATCTATTTAATAAATATTCATATCGAAAATTATTTTTAGTTGCGTTAGTAC
TCTTTACAATTGGTTCATTAATCTGTGCGATTTCAATGAATTTCCCAATCATGATGGTTGGACGTGTATTACAAGCCATT
GGTGCAGGTGTACTAATGCCATTAGGTTCAATTGTTATTATTACAATTTATCCACCTGAAAAACGTGGTGCTGCCATGGG
TACAATGGGTATCGCAATGATTTTAGCACCTGCAATTGGTCCAACATTATCAGGTTATATTGTACAAAATTACCATTGGA
ATGTGATGTTTTACGGTATGTTCATTATCGGGATTATCGCTATTTTAATTGGTTTTGTATGGTTTAAGTTATATCAATAT
ACAACAAACCCTAAAGCTGATATCCCAGGTATCATTTTTAGTACGATTGGTTTTGGTGCTTTGTTATATGGTTTCTCAGA
AGCTGGCAACAAAGGTTGGGGTTCAGTAGAGATAGAAACAATGTTTGCGATTGGTATTATCTTTATTATTCTATTCGTTA
TTAGAGAATTAAGAATGAAATCACCAATGTTGAATTTAGAAGTATTGAAATTCCCAACATTTACATTAACAACAATTATT
AATATGGTTGTAATGTTAAGTTTATATGGTGGTATGATTTTATTACCGATTTATTTACAAAATTTACGCGGATTCTCAGC
ATTAGATTCCGGATTGTTATTATTACCTGGTTCTCTAATTATGGGTCTACTAGGACCATTTGCAGGTAAATTGTTAGATA
CTATTGGTTTAAAACCATTAGCTATCTTTGGTATTGCTGTAATGACTTATGCAACATGGGAATTAACTAAATTAAATATG
GATACACCATATATGACAATCATGGGTATCTATGTACTTCGTTCATTTGGTATGGCATTTATAATGATGCCAATGGTAAC
TGCAGCTATTAATGCGTTACCGGGACGACTTGCCTCTCATGGTAATGCTTTCTTAAATACGATGCGTCAATTAGCAGGCT
CTATAGGTACAGCAATCTTAGTTACTGTAATGACAACACAAACTACACAACACTTATCAGCTTTTGGGGAAGAGTTAGAT
AAAACGAATCCTGTTGTACAAGATCATATGCGTGAATTAGCATCACAATATGGCGGACAAGAAGGCGCAATGAAAGTGTT
ACTACAATTTGTAAATAAACTAGCAACGGTTGAAGGTATTAATGATGCATTTATAGTTGCAACGATATTTAGCATCATCG
CCTTAATTTTATGTTTATTTTTACAAAGTAATAAAAAAGCAAAAGCTACAGCTCAAAAGTTAGATGCAGATAATAGTATC
AATCATGAATAA

Upstream 100 bases:

>100_bases
AATACCTGTTAAAATCACTCTAGAATCAGAACCGTCTAAACAAGTTGTTCCTGGAATGAACGCTGAAGTAAAAATCCATA
AAAATTAAGGGGGTCATTAA

Downstream 100 bases:

>100_bases
ATAAAATAAATTAATTGAAGTGTGACTAATCAAAAATTATGTTGTGGGGACATGATTTTTAAAGTATCGGTGCCAAATAT
GGTTATCGATACTTTTTTTA

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 643; Mature: 642

Protein sequence:

>643_residues
MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQSNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHS
QVSLNGYTKGSEKDQEAFTNNKDEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIGSLICAISMNFPIMMVGRVLQAI
GAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILAPAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILIGFVWFKLYQY
TTNPKADIPGIIFSTIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSPMLNLEVLKFPTFTLTTII
NMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGKLLDTIGLKPLAIFGIAVMTYATWELTKLNM
DTPYMTIMGIYVLRSFGMAFIMMPMVTAAINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELD
KTNPVVQDHMRELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSNKKAKATAQKLDADNSI
NHE

Sequences:

>Translated_643_residues
MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQSNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHS
QVSLNGYTKGSEKDQEAFTNNKDEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIGSLICAISMNFPIMMVGRVLQAI
GAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILAPAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILIGFVWFKLYQY
TTNPKADIPGIIFSTIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSPMLNLEVLKFPTFTLTTII
NMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGKLLDTIGLKPLAIFGIAVMTYATWELTKLNM
DTPYMTIMGIYVLRSFGMAFIMMPMVTAAINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELD
KTNPVVQDHMRELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSNKKAKATAQKLDADNSI
NHE
>Mature_642_residues
TTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQSNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQ
VSLNGYTKGSEKDQEAFTNNKDEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLNV
ALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIGSLICAISMNFPIMMVGRVLQAIG
AGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILAPAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILIGFVWFKLYQYT
TNPKADIPGIIFSTIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSPMLNLEVLKFPTFTLTTIIN
MVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGKLLDTIGLKPLAIFGIAVMTYATWELTKLNMD
TPYMTIMGIYVLRSFGMAFIMMPMVTAAINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDK
TNPVVQDHMRELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSNKKAKATAQKLDADNSIN
HE

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=476, Percent_Identity=28.3613445378151, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788710, Length=490, Percent_Identity=27.9591836734694, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI87081983, Length=413, Percent_Identity=27.6029055690073, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1790195, Length=438, Percent_Identity=28.0821917808219, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788392, Length=417, Percent_Identity=25.6594724220624, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322958, Length=544, Percent_Identity=21.875, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323735, Length=320, Percent_Identity=25.3125, Blast_Score=77, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=236, Percent_Identity=26.271186440678, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323520, Length=398, Percent_Identity=26.1306532663317, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=129, Percent_Identity=28.6821705426357, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 70979; Mature: 70848

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQSNQSKFKASDLDKTTD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
QSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNKDEEAVAAKNPESEEYKVN
HHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHCCCCCCCHHHCCCCCCCHHHHH
EKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLNVALPKINTEFNISASTGQWL
HHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHH
MTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIGSLICAISMNFPIMMVGRVLQAI
HHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILAPAIGPTLSGYIVQNYHWNVMFYGM
CCHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECEECHHHHHHH
FIIGIIAILIGFVWFKLYQYTTNPKADIPGIIFSTIGFGALLYGFSEAGNKGWGSVEIET
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHH
MFAIGIIFIILFVIRELRMKSPMLNLEVLKFPTFTLTTIINMVVMLSLYGGMILLPIYLQ
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
NLRGFSALDSGLLLLPGSLIMGLLGPFAGKLLDTIGLKPLAIFGIAVMTYATWELTKLNM
HCCCHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEECC
DTPYMTIMGIYVLRSFGMAFIMMPMVTAAINALPGRLASHGNAFLNTMRQLAGSIGTAIL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHH
VTVMTTQTTQHLSAFGEELDKTNPVVQDHMRELASQYGGQEGAMKVLLQFVNKLATVEGI
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
NDAFIVATIFSIIALILCLFLQSNKKAKATAQKLDADNSINHE
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQSNQSKFKASDLDKTTD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
QSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNKDEEAVAAKNPESEEYKVN
HHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHCCCCCCCHHHCCCCCCCHHHHH
EKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLNVALPKINTEFNISASTGQWL
HHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHH
MTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIGSLICAISMNFPIMMVGRVLQAI
HHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILAPAIGPTLSGYIVQNYHWNVMFYGM
CCHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECEECHHHHHHH
FIIGIIAILIGFVWFKLYQYTTNPKADIPGIIFSTIGFGALLYGFSEAGNKGWGSVEIET
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHH
MFAIGIIFIILFVIRELRMKSPMLNLEVLKFPTFTLTTIINMVVMLSLYGGMILLPIYLQ
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
NLRGFSALDSGLLLLPGSLIMGLLGPFAGKLLDTIGLKPLAIFGIAVMTYATWELTKLNM
HCCCHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEECC
DTPYMTIMGIYVLRSFGMAFIMMPMVTAAINALPGRLASHGNAFLNTMRQLAGSIGTAIL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHH
VTVMTTQTTQHLSAFGEELDKTNPVVQDHMRELASQYGGQEGAMKVLLQFVNKLATVEGI
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
NDAFIVATIFSIIALILCLFLQSNKKAKATAQKLDADNSINHE
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]