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The map label for this gene is lacR

Identifier: 57650784

GI number: 57650784

Start: 2269241

End: 2269996

Strand: Reverse

Name: lacR

Synonym: SACOL2188

Alternate gene names: 57650784

Gene position: 2269996-2269241 (Counterclockwise)

Preceding gene: 57650786

Following gene: 57650783

Centisome position: 80.8

GC content: 33.99

Gene sequence:

>756_bases
TTGAATAAACATGAACGTTTGGACGAAATTGCTAAACTAGTGAATAAAAAGGGCACGATAAGAACGAATGAAATCGTCGA
AGGTTTAAATGTGTCTGATATGACAGTTCGAAGAGATTTAATTGAATTGGAAAATAAAGGGATTTTAACGAAGATTCATG
GTGGTGCACGCAGTAATTCAACATTTCAGTATAAAGAAATATCGCATAAAGAAAAACATACCCGTCAAATTGCAGAAAAA
CGATTTATAGCTAAAAAAGCTGCATCATTAATTGAAGATGGGGATACTTTGTTTTTCGGACCAGGAACAACAGTGGAACT
ATTAGCAGAAGAAGTCAATCATCATACGCTCACAATTATTACGAATTGTTTGCCGGTGTATAAAATTTTGTTAGAAAAAC
AAACAGCACATTTTCGTGTCTATTTAATTGGTGGTGAAATGCGCCATATTACAGAAGCATTTGTAGGTGAAATGGCGAAC
GCCATGTTGGAAAAACTAAGATTTAGCAAGATGTTCTTTAGTAGTAACGCAGTAAATAAAGGTGCTGTGATGACATCTAC
ATTAGATGAAGCCTATACGCAACAACTGGCACTAAGTAATTCAATTGAAAAATACTTGTTAATCGATCATACGAAAGTTG
GCAAAGAAGATTTTACATCATTTTGTCAGTTAAACGAATTGACTGCAGTGGTCATGGACTATGAAGATGAAGAAAAAGTA
GAAACGATTAAAACATATATTGAAGTAGTCGACTAA

Upstream 100 bases:

>100_bases
TTTACGCAATTTTTATTATAGAATAAGCATGTATATTAAAATTGATGTCTAAACATAAAGGATAATGACCCATCGCCTAA
ATAAAGGAGGCAATTTTACA

Downstream 100 bases:

>100_bases
ATTAAAAGTATAACTGCATTGTTAAAATATGTATCTATATGCTCATAAGTGAATGTGAATTTGCATTCATTTATGGGCTA
TTTTTTATGCAAATTTATGT

Product: lactose phosphotransferase system repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MNKHERLDEIAKLVNKKGTIRTNEIVEGLNVSDMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEK
RFIAKKAASLIEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMRHITEAFVGEMAN
AMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYLLIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKV
ETIKTYIEVVD

Sequences:

>Translated_251_residues
MNKHERLDEIAKLVNKKGTIRTNEIVEGLNVSDMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEK
RFIAKKAASLIEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMRHITEAFVGEMAN
AMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYLLIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKV
ETIKTYIEVVD
>Mature_251_residues
MNKHERLDEIAKLVNKKGTIRTNEIVEGLNVSDMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEK
RFIAKKAASLIEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMRHITEAFVGEMAN
AMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYLLIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKV
ETIKTYIEVVD

Specific function: Repressor of the lactose catabolism operon. Galactose-6- phosphate is the inducer

COG id: COG1349

COG function: function code KG; Transcriptional regulators of sugar metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH deoR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1789829, Length=247, Percent_Identity=31.9838056680162, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI87082344, Length=255, Percent_Identity=30.1960784313725, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI226510968, Length=230, Percent_Identity=29.5652173913043, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1789059, Length=235, Percent_Identity=26.8085106382979, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1789519, Length=190, Percent_Identity=31.5789473684211, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788069, Length=236, Percent_Identity=25.4237288135593, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787063, Length=251, Percent_Identity=26.6932270916335, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789170, Length=251, Percent_Identity=26.2948207171315, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790635, Length=234, Percent_Identity=24.3589743589744, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787540, Length=259, Percent_Identity=26.6409266409266, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): LACR_STAA8 (P0A0Q0)

Other databases:

- EMBL:   M32103
- EMBL:   CP000253
- PIR:   A44506
- RefSeq:   YP_500923.1
- ProteinModelPortal:   P0A0Q0
- STRING:   P0A0Q0
- EnsemblBacteria:   EBSTAT00000029504
- GeneID:   3919019
- GenomeReviews:   CP000253_GR
- KEGG:   sao:SAOUHSC_02456
- eggNOG:   COG1349
- GeneTree:   EBGT00050000024434
- HOGENOM:   HBG694151
- ProtClustDB:   CLSK2460867
- BioCyc:   SAUR93061:SAOUHSC_02456-MONOMER
- GO:   GO:0005622
- InterPro:   IPR014036
- InterPro:   IPR001034
- InterPro:   IPR018356
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00037
- SMART:   SM00420

Pfam domain/function: PF00455 DeoR; PF08220 HTH_DeoR

EC number: NA

Molecular weight: Translated: 28549; Mature: 28549

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00894 HTH_DEOR_1; PS51000 HTH_DEOR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKHERLDEIAKLVNKKGTIRTNEIVEGLNVSDMTVRRDLIELENKGILTKIHGGARSNS
CCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCC
TFQYKEISHKEKHTRQIAEKRFIAKKAASLIEDGDTLFFGPGTTVELLAEEVNHHTLTII
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCEEHHH
TNCLPVYKILLEKQTAHFRVYLIGGEMRHITEAFVGEMANAMLEKLRFSKMFFSSNAVNK
HHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GAVMTSTLDEAYTQQLALSNSIEKYLLIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKV
CCCHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCHHHHHHHHHHHHHHEEEECCCCHHHH
ETIKTYIEVVD
HHHHHHHHHCC
>Mature Secondary Structure
MNKHERLDEIAKLVNKKGTIRTNEIVEGLNVSDMTVRRDLIELENKGILTKIHGGARSNS
CCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCC
TFQYKEISHKEKHTRQIAEKRFIAKKAASLIEDGDTLFFGPGTTVELLAEEVNHHTLTII
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCEEHHH
TNCLPVYKILLEKQTAHFRVYLIGGEMRHITEAFVGEMANAMLEKLRFSKMFFSSNAVNK
HHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GAVMTSTLDEAYTQQLALSNSIEKYLLIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKV
CCCHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCHHHHHHHHHHHHHHEEEECCCCHHHH
ETIKTYIEVVD
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2163387