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The map label for this gene is nrgA [H]

Identifier: 57650715

GI number: 57650715

Start: 2089985

End: 2091235

Strand: Reverse

Name: nrgA [H]

Synonym: SACOL2031

Alternate gene names: 57650715

Gene position: 2091235-2089985 (Counterclockwise)

Preceding gene: 57650716

Following gene: 57650714

Centisome position: 74.44

GC content: 32.93

Gene sequence:

>1251_bases
ATGAATCTTAACGATACGATATTTATGTTTTTGTGTACATTATTAGTTTGGTTAATGACACCAGGATTAAGTTTATTTTA
TGGTGGGTTAGTTGAATCTAAAAATGCGCTTAATACTGTCATGCAAAGTATGGCAGCAATTGTGCTTGTTACATTTGTAT
GGATAACAGTTGGTTTTACAATTAGTTTTGGGAATGGGAATTTATGGTTCGGAAATTGGGAATATACTTTTCTTAATCAT
GTAGGTTTTGCGACTCAAGAAGATATTAGCCCACATATTCCTTTCGCTTTGTTTATGTTATTTCAAATGATGTTTTGTAC
GATTGCAATTTCTATTTTATCTGGTTCAATCGCTGAGAAAATGAAGTTTATTCCTTATTTATTATTCGTAGTAATATGGA
CTGCTCTTGTATACAGTCCAGTAGCACATTGGGTTTGGGGCGGCGGTTGGATTAACAAACTCGGTGTATTAGATTTCGCT
GGAGGTACGGTTGTTCATATTACATCAGGTGTTTCTGGTTTAGTATTAGCTATTATGATTGGAAAAGGAAACAAACATTC
TGAATCAACACCACATAATCTTATCATTACGTTGATTGGCGGTATATTCGTGTGGATTGGTTGGTATGGATTTAATGTAG
GTAGTGCTTTTACATTTGATAATATTGCGATGCTTGCATTTACAAATACTGTCATTTCAGCCAGTGCAGGTGCTATAGGT
TGGTTAATTTTAGAATATATTTTTAAAAAGACGACAAGTTTACTTGGACTTTTACTCGGTGCATTAGCAGGATTAGTTGT
CATTACTCCTGCAGCAGGATATGTAACATATCTTAGTGCAACAATAATGGCTTTAATAGGAGGTATCTGTTGTTATATTG
TCATTAATTACATCAAGGTAAAACTAAAATATCATGATGCATTAGATGCATTTGGTATTCATGGTGTTGGTGGTATTATT
GGTGCTGTTTTAACAGCAGTTTTCCAAAGTAAAAAAGCCAATCCTGACATTGAGAATGGCTTTATTTATACTGGTGACAT
ACATATTATACTTGTACAAATATTATGTGTAACAGCAGTTGTAATTTTTAGTATAGTCATGACGTTTATTATTGCGAAAG
TAATTAAATTAATTACACCATTATCTGTTACGGAACAAGAAACGAATATAGGATTAGACAAGATTGTTCACGGTGAACAT
GCTTACTTTGAAGGTGAGCTAAATAGATTCAATAAACATATTCGATATTAG

Upstream 100 bases:

>100_bases
TATAGTGTGGCACATTTTATTCCAAAAGATGTAATAAAACTTAACGCATTTTTGCTTTTTATAAATTGTCAGATTATTAT
GAAAAAAGGGAGTGGTAAGT

Downstream 100 bases:

>100_bases
AATATATTTACATAGAATATTCATTGTCCTGACATTTAACTAAAGGTTGATGTTGGGACATTTTGTTATACAAAAGTTTT
ATTTTGAAATCTTTTTATGA

Product: ammonium transporter family protein

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVESKNALNTVMQSMAAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNH
VGFATQEDISPHIPFALFMLFQMMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDNIAMLAFTNTVISASAGAIG
WLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVTYLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGII
GAVLTAVFQSKKANPDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGEH
AYFEGELNRFNKHIRY

Sequences:

>Translated_416_residues
MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVESKNALNTVMQSMAAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNH
VGFATQEDISPHIPFALFMLFQMMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDNIAMLAFTNTVISASAGAIG
WLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVTYLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGII
GAVLTAVFQSKKANPDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGEH
AYFEGELNRFNKHIRY
>Mature_416_residues
MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVESKNALNTVMQSMAAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNH
VGFATQEDISPHIPFALFMLFQMMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDNIAMLAFTNTVISASAGAIG
WLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVTYLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGII
GAVLTAVFQSKKANPDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGEH
AYFEGELNRFNKHIRY

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=403, Percent_Identity=40.1985111662531, Blast_Score=278, Evalue=6e-76,
Organism=Caenorhabditis elegans, GI133931100, Length=427, Percent_Identity=25.0585480093677, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32564042, Length=416, Percent_Identity=26.2019230769231, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17550182, Length=438, Percent_Identity=27.3972602739726, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17550180, Length=431, Percent_Identity=25.5220417633411, Blast_Score=114, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6324187, Length=412, Percent_Identity=33.7378640776699, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6325396, Length=416, Percent_Identity=32.6923076923077, Blast_Score=212, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6321559, Length=416, Percent_Identity=32.4519230769231, Blast_Score=210, Evalue=3e-55,
Organism=Drosophila melanogaster, GI161078314, Length=414, Percent_Identity=28.5024154589372, Blast_Score=128, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45481; Mature: 45481

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVESKNALNTVMQSMAAIVLVTFVWITVGFT
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHE
ISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQMMFCTIAISILSGSIAEK
EEECCCEEEECCCEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFAGGTVVHITSGVSGLVLAIMI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEECCCEEEEEECCHHHHHHHEEE
GKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDNIAMLAFTNTVISASAGAIG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCHHHH
WLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVTYLSATIMALIGGICCYIVINYIKV
HHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKANPDIENGFIYTGDIHIILVQILCVTAV
EEEEHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHH
VIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGEHAYFEGELNRFNKHIRY
HHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHCCCCCCEEHHHHHHHHHHCCC
>Mature Secondary Structure
MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVESKNALNTVMQSMAAIVLVTFVWITVGFT
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHE
ISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQMMFCTIAISILSGSIAEK
EEECCCEEEECCCEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFAGGTVVHITSGVSGLVLAIMI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEECCCEEEEEECCHHHHHHHEEE
GKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDNIAMLAFTNTVISASAGAIG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCHHHH
WLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVTYLSATIMALIGGICCYIVINYIKV
HHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKANPDIENGFIYTGDIHIILVQILCVTAV
EEEEHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHH
VIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGEHAYFEGELNRFNKHIRY
HHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHCCCCCCEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]