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The map label for this gene is splE

Identifier: 57650600

GI number: 57650600

Start: 1917447

End: 1918163

Strand: Reverse

Name: splE

Synonym: SACOL1865

Alternate gene names: 57650600

Gene position: 1918163-1917447 (Counterclockwise)

Preceding gene: 57650601

Following gene: 57650599

Centisome position: 68.28

GC content: 34.59

Gene sequence:

>717_bases
ATGAATAAAAATATAATCATCAAAAGTATTGCAGCATTGACGATTTTAACATCAGTGACTGGCGTCGGCACAACAGTGGT
TGAGGGTATTCAACAAACGGCTAAAGCTGAACATAATGTGAAACTAATCAAAAATACTAATGTAGCACCATACAATGGTG
TCGTTTCGATAGGATCTGGAACAGGTTTCATTGTCGGTAAAAATACAATTGTTACCAACAAGCATGTCGTTGCAGGTATG
GAAATTGGTGCACATATTATAGCGCATCCCAATGGTGAATATAATAATGGCGGATTTTATAAAGTTAAAAAAATTGTCCG
TTATTCAGGTCAAGAAGATATTGCCATTCTACATGTGGAAGATAAAGCTGTTCATCCAAAAAACAGGAATTTTAAAGATT
ACACAGGCATTTTAAAAATAGCATCAGAAGCTAAAGAAAATGAACGCATTTCAATTGTTGGCTATCCAGAACCATATATA
AATAAATTTCAAATGTATGAGTCAACAGGAAAAGTGCTGTCAGTTAAAGGCAACATGATTATTACTGATGCTTTCGTAGA
ACCAGGCAACTCAGGTTCAGCTGTATTTAACAGTAAATACGAAGTTGTAGGTGTTCACTTTGGTGGAAACGGCCCTGGAA
ATAAAAGTACAAAAGGATATGGTGTTTATTTCTCTCCTGAAATTAAGAAATTCATTGCAGATAACACAGATAAATAA

Upstream 100 bases:

>100_bases
TTTAAACATCTCTGTGATTCTATTTATTCGAAATGATTTAAAAAATAAAACTTCAAAAACCTAACCTTATATTTATACGA
ATACTTAGAGGAGCACAAAA

Downstream 100 bases:

>100_bases
ATCCTTACATAGATAAATGATTTTAAAAATTAACAACAAACTCAACAATTCAAATCATCTCTGTGATTCCATTTATTCGA
AATGATTAAAAAAAATAAAA

Product: serine protease SplE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MNKNIIIKSIAALTILTSVTGVGTTVVEGIQQTAKAEHNVKLIKNTNVAPYNGVVSIGSGTGFIVGKNTIVTNKHVVAGM
EIGAHIIAHPNGEYNNGGFYKVKKIVRYSGQEDIAILHVEDKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYI
NKFQMYESTGKVLSVKGNMIITDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPGNKSTKGYGVYFSPEIKKFIADNTDK

Sequences:

>Translated_238_residues
MNKNIIIKSIAALTILTSVTGVGTTVVEGIQQTAKAEHNVKLIKNTNVAPYNGVVSIGSGTGFIVGKNTIVTNKHVVAGM
EIGAHIIAHPNGEYNNGGFYKVKKIVRYSGQEDIAILHVEDKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYI
NKFQMYESTGKVLSVKGNMIITDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPGNKSTKGYGVYFSPEIKKFIADNTDK
>Mature_238_residues
MNKNIIIKSIAALTILTSVTGVGTTVVEGIQQTAKAEHNVKLIKNTNVAPYNGVVSIGSGTGFIVGKNTIVTNKHVVAGM
EIGAHIIAHPNGEYNNGGFYKVKKIVRYSGQEDIAILHVEDKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYI
NKFQMYESTGKVLSVKGNMIITDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPGNKSTKGYGVYFSPEIKKFIADNTDK

Specific function: Unknown

COG id: COG3591

COG function: function code E; V8-like Glu-specific endopeptidase

Gene ontology:

Cell location: Secreted

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S1B family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPLE_STAA3 (Q2FFT3)

Other databases:

- EMBL:   CP000255
- RefSeq:   YP_494445.1
- HSSP:   Q53782
- ProteinModelPortal:   Q2FFT3
- SMR:   Q2FFT3
- STRING:   Q2FFT3
- EnsemblBacteria:   EBSTAT00000038336
- GeneID:   3913007
- GenomeReviews:   CP000255_GR
- KEGG:   saa:SAUSA300_1754
- eggNOG:   COG3591
- GeneTree:   EBGT00050000023734
- HOGENOM:   HBG693141
- OMA:   CTVISIN
- ProtClustDB:   CLSK885576
- GO:   GO:0006508
- InterPro:   IPR009003
- InterPro:   IPR000126
- InterPro:   IPR001254
- InterPro:   IPR008256
- InterPro:   IPR008353
- PRINTS:   PR01774
- PRINTS:   PR00839

Pfam domain/function: PF00089 Trypsin; SSF50494 Pept_Ser_Cys

EC number: NA

Molecular weight: Translated: 25680; Mature: 25680

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS00672 V8_HIS; PS00673 V8_SER

Important sites: ACT_SITE 75-75 ACT_SITE 113-113 ACT_SITE 191-191

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNIIIKSIAALTILTSVTGVGTTVVEGIQQTAKAEHNVKLIKNTNVAPYNGVVSIGSG
CCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEECCC
TGFIVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKVKKIVRYSGQEDIAILHVE
CEEEEECCEEEECCEEEEEEEECEEEEECCCCCCCCCCHHEEEHHHHCCCCCCEEEEEEE
DKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYINKFQMYESTGKVLSVKGNMI
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHEEEECCCCCEEEEECCEE
ITDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPGNKSTKGYGVYFSPEIKKFIADNTDK
EEEEEECCCCCCCEEECCCEEEEEEEECCCCCCCCCCCCEEEEECHHHHHHHCCCCCC
>Mature Secondary Structure
MNKNIIIKSIAALTILTSVTGVGTTVVEGIQQTAKAEHNVKLIKNTNVAPYNGVVSIGSG
CCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEECCC
TGFIVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKVKKIVRYSGQEDIAILHVE
CEEEEECCEEEECCEEEEEEEECEEEEECCCCCCCCCCHHEEEHHHHCCCCCCEEEEEEE
DKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYINKFQMYESTGKVLSVKGNMI
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHEEEECCCCCEEEEECCEE
ITDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPGNKSTKGYGVYFSPEIKKFIADNTDK
EEEEEECCCCCCCEEECCCEEEEEEEECCCCCCCCCCCCEEEEECHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA