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The map label for this gene is hsdS [C]

Identifier: 57650596

GI number: 57650596

Start: 1913466

End: 1914665

Strand: Reverse

Name: hsdS [C]

Synonym: SACOL1861

Alternate gene names: 57650596

Gene position: 1914665-1913466 (Counterclockwise)

Preceding gene: 57650597

Following gene: 57650595

Centisome position: 68.15

GC content: 30.08

Gene sequence:

>1200_bases
ATGAGTAATACACAAAAGAAAAATGTGCCAGAATTGAGATTCCCAGGATTTGAAGGCGAATGGGAAGAGAAGAAGTTAGG
GAATCTTACTACCAAAATAGGTAGTGGAAAGACTCCCAAAGGTGGAAGTGAAAACTATACAAACAAAGGCATACCATTTT
TAAGGAGTCAAAATATTAGAAATGGTAAATTAAATCTTAATGACTTAGTTTATATTAGTAAAGATATAGATGATGAGATG
AAAAATAGTAGAACGTACTATGGTGATGTTCTTTTAAATATTACAGGAGCATCAATAGGTAGAACAGCCATTAATTCGAT
AGTTGAAACGCATGCTAATTTAAATCAACATGTATGTATTATTAGATTGAAAAAAGAGTATTATTATAATTTTTTTGGAC
AGTATCTATTATCAAGAAAAGGTAAAAGGAAAATTTTCCTTGCACAAAGTGGAGGTAGTCGAGAAGGTCTAAACTTCAAA
GAAATTGCTAATTTAAAAATCTTCACCCCAACTATATTTGAAGAACAGCAAAAAATAGGCAAGTTCTTCAGCAAACTCGA
CCGACAAATTGAATTAGAAGAACAAAAACTTGAATTGCTTCAACAACAGAAAAAAGGCTATATGCAGAAAATCTTCACAC
AAGAATTGCGATTCAAAGATGAGAATGGTGAAGAATATCCAGAGTGGGAAAACAAATTCATAAAAGACATCTTTATCTTT
GAAAATAATAGAAGAAAACCAATTACTTCTTCATTAAGAGAAAAGGGGTTATACCCTTACTATGGTGCAACTGGAATTAT
TGATTACGTAAAAGATTATTTATTCAATAATGAAGAACGATTACTAATAGGAGAAGATGGTGCAAAATGGGGGCAGTTTG
AGACGAGTAGCTTTATTGCTAATGGGCAATACTGGGTAAATAATCATGCGCATGTAGTTAAAAGTAATGATCATAATTTG
TTTTTTATGAATTATTATTTAAATTTTAAAGAACTACGAGCATTTGTCACAGGTAATGCACCAGCTAAATTAACTCATGC
GAACTTATGCAATATAAATCTTAAAATACCTTGTCTCACTGAACAAGATAAAGTAAGTGCATTGTTAAAATCTATAGACA
ATAAAATGAATAATCAAATGAATAGAATTGAGTTATTAAAAGAACGTAAAAAAGGACTATTACAAAAAATGTTTATTTAA

Upstream 100 bases:

>100_bases
TTAGATCAAGTCCAACAAGATTTGAAAAATATCGATAAAGAAATCGCAGAAATTGAGCAAGAAATCAATGCATACCTGAA
AGAACTTGGGGTGTTGAAAG

Downstream 100 bases:

>100_bases
CCCTGATAACAACCTATTAATTGAAATGATAAAAATCCTATGAATAAATTGTAATATATTAATATTGAGACTATTATAAT
TTGGGGTTTATTTTTTTAGA

Product: type I restriction-modification enzyme, S subunit, EcoA family protein

Products: NA

Alternate protein names: S.MjaXIP; Type I restriction enzyme MjaXIP specificity protein; S protein [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSNTQKKNVPELRFPGFEGEWEEKKLGNLTTKIGSGKTPKGGSENYTNKGIPFLRSQNIRNGKLNLNDLVYISKDIDDEM
KNSRTYYGDVLLNITGASIGRTAINSIVETHANLNQHVCIIRLKKEYYYNFFGQYLLSRKGKRKIFLAQSGGSREGLNFK
EIANLKIFTPTIFEEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFTQELRFKDENGEEYPEWENKFIKDIFIF
ENNRRKPITSSLREKGLYPYYGATGIIDYVKDYLFNNEERLLIGEDGAKWGQFETSSFIANGQYWVNNHAHVVKSNDHNL
FFMNYYLNFKELRAFVTGNAPAKLTHANLCNINLKIPCLTEQDKVSALLKSIDNKMNNQMNRIELLKERKKGLLQKMFI

Sequences:

>Translated_399_residues
MSNTQKKNVPELRFPGFEGEWEEKKLGNLTTKIGSGKTPKGGSENYTNKGIPFLRSQNIRNGKLNLNDLVYISKDIDDEM
KNSRTYYGDVLLNITGASIGRTAINSIVETHANLNQHVCIIRLKKEYYYNFFGQYLLSRKGKRKIFLAQSGGSREGLNFK
EIANLKIFTPTIFEEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFTQELRFKDENGEEYPEWENKFIKDIFIF
ENNRRKPITSSLREKGLYPYYGATGIIDYVKDYLFNNEERLLIGEDGAKWGQFETSSFIANGQYWVNNHAHVVKSNDHNL
FFMNYYLNFKELRAFVTGNAPAKLTHANLCNINLKIPCLTEQDKVSALLKSIDNKMNNQMNRIELLKERKKGLLQKMFI
>Mature_398_residues
SNTQKKNVPELRFPGFEGEWEEKKLGNLTTKIGSGKTPKGGSENYTNKGIPFLRSQNIRNGKLNLNDLVYISKDIDDEMK
NSRTYYGDVLLNITGASIGRTAINSIVETHANLNQHVCIIRLKKEYYYNFFGQYLLSRKGKRKIFLAQSGGSREGLNFKE
IANLKIFTPTIFEEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFTQELRFKDENGEEYPEWENKFIKDIFIFE
NNRRKPITSSLREKGLYPYYGATGIIDYVKDYLFNNEERLLIGEDGAKWGQFETSSFIANGQYWVNNHAHVVKSNDHNLF
FMNYYLNFKELRAFVTGNAPAKLTHANLCNINLKIPCLTEQDKVSALLKSIDNKMNNQMNRIELLKERKKGLLQKMFI

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 46469; Mature: 46338

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTQKKNVPELRFPGFEGEWEEKKLGNLTTKIGSGKTPKGGSENYTNKGIPFLRSQNIR
CCCCCCCCCCCCCCCCCCCCCHHHHHCCHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
NGKLNLNDLVYISKDIDDEMKNSRTYYGDVLLNITGASIGRTAINSIVETHANLNQHVCI
CCCCCCCEEEEEECCCCHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEE
IRLKKEYYYNFFGQYLLSRKGKRKIFLAQSGGSREGLNFKEIANLKIFTPTIFEEQQKIG
EEEEHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHCCEEEECCHHHHHHHHHH
KFFSKLDRQIELEEQKLELLQQQKKGYMQKIFTQELRFKDENGEEYPEWENKFIKDIFIF
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHEEEEE
ENNRRKPITSSLREKGLYPYYGATGIIDYVKDYLFNNEERLLIGEDGAKWGQFETSSFIA
ECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEE
NGQYWVNNHAHVVKSNDHNLFFMNYYLNFKELRAFVTGNAPAKLTHANLCNINLKIPCLT
CCEEEECCCEEEEECCCCCEEEEEEECCHHHHHHHHCCCCCCEEEECEEEEEEEEEECCC
EQDKVSALLKSIDNKMNNQMNRIELLKERKKGLLQKMFI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNTQKKNVPELRFPGFEGEWEEKKLGNLTTKIGSGKTPKGGSENYTNKGIPFLRSQNIR
CCCCCCCCCCCCCCCCCCCCHHHHHCCHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
NGKLNLNDLVYISKDIDDEMKNSRTYYGDVLLNITGASIGRTAINSIVETHANLNQHVCI
CCCCCCCEEEEEECCCCHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEE
IRLKKEYYYNFFGQYLLSRKGKRKIFLAQSGGSREGLNFKEIANLKIFTPTIFEEQQKIG
EEEEHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHCCEEEECCHHHHHHHHHH
KFFSKLDRQIELEEQKLELLQQQKKGYMQKIFTQELRFKDENGEEYPEWENKFIKDIFIF
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHEEEEE
ENNRRKPITSSLREKGLYPYYGATGIIDYVKDYLFNNEERLLIGEDGAKWGQFETSSFIA
ECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEE
NGQYWVNNHAHVVKSNDHNLFFMNYYLNFKELRAFVTGNAPAKLTHANLCNINLKIPCLT
CCEEEECCCEEEEECCCCCEEEEEEECCHHHHHHHHCCCCCCEEEECEEEEEEEEEECCC
EQDKVSALLKSIDNKMNNQMNRIELLKERKKGLLQKMFI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]