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The map label for this gene is metK [H]

Identifier: 57650575

GI number: 57650575

Start: 1891567

End: 1892760

Strand: Reverse

Name: metK [H]

Synonym: SACOL1837

Alternate gene names: 57650575

Gene position: 1892760-1891567 (Counterclockwise)

Preceding gene: 57650577

Following gene: 57652082

Centisome position: 67.37

GC content: 37.69

Gene sequence:

>1194_bases
ATGTTAAATAACAAACGATTATTTACTTCAGAGTCTGTTACAGAAGGACACCCAGATAAAATCGCTGACCAAGTGTCAGA
TGCAATATTAGATGCTATTTTAAAAGACGATCCAAATGCACGTGTAGCTTGTGAAACAACTGTTACAACAGGTATGGCAT
TGATCGCCGGCGAAATTTCTACAACAACATATGTTGATATTCCGAAAGTTGTTAGAGAAACAATTAAAGAAATTGGATAC
ACAAGAGCAAAATATGGTTATGATTATGAAACAATGGCAATTTTAACTGCGATTGATGAACAATCACCTGACATTGCACA
AGGCGTGGATAAAGCATTAGAGTATCGTGATAAAGATAGCGAAGAAGAAATTGAAGCAACAGGTGCAGGTGACCAAGGTT
TAATGTTTGGTTACGCAACGAATGAAACAGAGACGTATATGCCTCTAGCAATTTATTTATCACATCAATTGGCAAAACGT
TTATCAGATGTGCGTAAAGATGGTACGTTAAATTATTTACGACCAGACGGTAAAGTTCAGGTCACTGTTGAATATGATGA
AAATGATAACCCAGTACGTATTGATACGATTGTCGTATCAACACAACATGCTGAGGATGTGACACTTGAACAAATTCAAG
AAGACATTAAAGCACATGTCATTTATCCAACTGTTCCAGAAAACTTGATTAATGAACAAACTAAATTCTACATCAATCCT
ACAGGACGCTTTGTAATCGGTGGACCTCAAGGTGATGCAGGATTAACAGGACGTAAAATCATTGTTGATACGTACGGTGG
CTATGCACGTCATGGTGGCGGATGCTTCAGTGGTAAGGATCCTACAAAAGTAGACCGTTCAGCTGCATACGCTGCACGTT
ATGTGGCTAAAAATATTGTTGCAGCAGGTTTAGCAGATCAATGTGAAGTACAATTGGCATATGCGATTGGTGTTGCAGAA
CCAGTGTCAATTGCAATTGATACATTTGGAACAGGTAAAGTTTCTGAAGGGCAACTTGTCGAAGCAGTTAGAAAACACTT
TGACCTAAGACCTGCAGGTATTATTAAAATGCTTGATTTGAAACAACCGATTTACAAACAAACTGCTGCTTATGGTCATT
TTGGACGTACAGATGTCTTATTCCCATGGGAAAAATTAGACAAAGTGGAAGAATTAAAAGACGCAGTAAAATAA

Upstream 100 bases:

>100_bases
GGACGTATTAGGCCTTCTCTCTATATTAATAGTTAGAAGGTCTTTTTTATTTAGCTCACAGAGAGAGAATTTTCGTAATA
TAAATTTAAAGGAGCAAACT

Downstream 100 bases:

>100_bases
TAAGATGAATTGAATATAGCTAAATAAAAGATTTTCGGTATATAAATGATAATATGTATCGAAAATCTTTAGCTTTTCTA
TTATAATTAAAGTTATTCAT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVACETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGY
TRAKYGYDYETMAILTAIDEQSPDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKAHVIYPTVPENLINEQTKFYINP
TGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCFSGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAE
PVSIAIDTFGTGKVSEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEELKDAVK

Sequences:

>Translated_397_residues
MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVACETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGY
TRAKYGYDYETMAILTAIDEQSPDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKAHVIYPTVPENLINEQTKFYINP
TGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCFSGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAE
PVSIAIDTFGTGKVSEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEELKDAVK
>Mature_397_residues
MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVACETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGY
TRAKYGYDYETMAILTAIDEQSPDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKAHVIYPTVPENLINEQTKFYINP
TGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCFSGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAE
PVSIAIDTFGTGKVSEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEELKDAVK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=381, Percent_Identity=58.7926509186352, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI5174529, Length=381, Percent_Identity=58.2677165354331, Blast_Score=433, Evalue=1e-121,
Organism=Escherichia coli, GI1789311, Length=391, Percent_Identity=63.1713554987212, Blast_Score=506, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17538494, Length=388, Percent_Identity=54.639175257732, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25145633, Length=397, Percent_Identity=54.6599496221663, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17538498, Length=388, Percent_Identity=54.8969072164949, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17551082, Length=387, Percent_Identity=55.0387596899225, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32565905, Length=388, Percent_Identity=50.2577319587629, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI32565911, Length=337, Percent_Identity=53.1157270029674, Blast_Score=355, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI25145635, Length=346, Percent_Identity=52.6011560693642, Blast_Score=353, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI32565903, Length=339, Percent_Identity=51.3274336283186, Blast_Score=335, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI32565909, Length=86, Percent_Identity=55.8139534883721, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=68.1818181818182, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=382, Percent_Identity=56.282722513089, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6320710, Length=388, Percent_Identity=55.4123711340206, Blast_Score=426, Evalue=1e-120,
Organism=Drosophila melanogaster, GI45552159, Length=387, Percent_Identity=54.7803617571059, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580529, Length=387, Percent_Identity=54.7803617571059, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580537, Length=387, Percent_Identity=54.7803617571059, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580533, Length=387, Percent_Identity=54.7803617571059, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580535, Length=387, Percent_Identity=54.7803617571059, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580531, Length=387, Percent_Identity=54.7803617571059, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580527, Length=387, Percent_Identity=54.5219638242894, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580525, Length=387, Percent_Identity=54.5219638242894, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580541, Length=375, Percent_Identity=53.0666666666667, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580539, Length=126, Percent_Identity=56.3492063492063, Blast_Score=142, Evalue=4e-34,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43642; Mature: 43642

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVACETTVTTGMALIAGEIS
CCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCEEEEECCCC
TTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQSPDIAQGVDKALEYRDKDS
CCEEECCHHHHHHHHHHCCCCHHHCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHCCCCC
EEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKRLSDVRKDGTLNYLRPDGKVQ
HHHHHHCCCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEE
VTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKAHVIYPTVPENLINEQTKFYINP
EEEEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHEEEEECCCCHHHCCCCCEEEECC
TGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCFSGKDPTKVDRSAAYAARYVAKNIV
CCCEEEECCCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKVSEGQLVEAVRKHFDLRPAGIIKMLDL
HHCCCCHHHEEEEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHEEHHC
KQPIYKQTAAYGHFGRTDVLFPWEKLDKVEELKDAVK
CCHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVACETTVTTGMALIAGEIS
CCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCEEEEECCCC
TTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQSPDIAQGVDKALEYRDKDS
CCEEECCHHHHHHHHHHCCCCHHHCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHCCCCC
EEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKRLSDVRKDGTLNYLRPDGKVQ
HHHHHHCCCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEE
VTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKAHVIYPTVPENLINEQTKFYINP
EEEEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHEEEEECCCCHHHCCCCCEEEECC
TGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCFSGKDPTKVDRSAAYAARYVAKNIV
CCCEEEECCCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKVSEGQLVEAVRKHFDLRPAGIIKMLDL
HHCCCCHHHEEEEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHEEHHC
KQPIYKQTAAYGHFGRTDVLFPWEKLDKVEELKDAVK
CCHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA