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The map label for this gene is ytsJ [H]

Identifier: 57650572

GI number: 57650572

Start: 1786938

End: 1788167

Strand: Reverse

Name: ytsJ [H]

Synonym: SACOL1749

Alternate gene names: 57650572

Gene position: 1788167-1786938 (Counterclockwise)

Preceding gene: 57650573

Following gene: 57650571

Centisome position: 63.65

GC content: 35.85

Gene sequence:

>1230_bases
ATGTCATTAAGAGATGAAGCATTGGAAATGCACAAACGTAATCAAGGTAAATTAGAAGTTAAACCAAATGTAAAAGTTAC
TAATAAAGAGGAATTAAGTTTAGCATACTCACCTGGCGTTGCTGAACCGTGTAAAGATATTTATGAAGATAAAAGAAAAG
TATATGATTACACAATTAAAGGAAATACAGTTGCAGTTATTACTGATGGAACAGCGGTATTAGGTTTAGGTAACATTGGA
CCTGAAGCAAGTATTCCTGTAATGGAAGGTAAAGCAGTATTATTCAAAAGCTTCGCTGGTATCAATGGGGTGCCTATTGC
GTTAAATACAACTGATACCGAAGAAATCATTAAAACAGTTAAGTTGTTAGAACCTAATTATGGTGGTATTAATTTAGAGG
ATATTTCGGCACCACGTTGTTTTGAAATTGAAGAACGATTGAAAAAAGAAACTAATATTCCGGTATTCCATGACGATCAA
CATGGTACAGCAATTGTAACATTGGCAGGTTTGGTAAATGCATTGAGAGTTGTTAACAAAGATATTGCTAAAATAAAAGT
TGTACTAAATGGTGCTGGTGCAGCAGGAATAGCCATTGTTAAATTACTATACGCGTATGGTGTAAGAAATATGGTTATGT
GTGACTCAAGAGGCGCAATTTTTGAAGGACGTTCATATGGTATGAATCCTACGAAAGATGTTGTAGCAAAATGGACAAAT
AAAGATAAGATTGAAGGGTCTTTAGAAGAAGTCGTAAAAGACGCAGATGTATTTATCGGGGTTTCTGTAGCTAATGCGCT
GTCACAAGATATGGTTAAGAGTATGGCAGATAATCCAATTATATTTGCAATGGCTAATCCAAATCCTGAAATAATACCTG
ATGATGCCAAAGCGGCAGGTGCACGAGTTGTTGGTACAGGACGTTCAGACTATCCTAACCAAATTAATAATGTATTAGCT
TTCCCTGGTATTTTTAGAGGTGCATTAGAGGTTGAAGCTACACATATAAATGAAGAAATGAAAAAGGCAGCTGTAGAAGC
GATTGCTGATTTAATCGATAGTTCTGAATTAAATGAAGACTACTGTATCCCAGGACCGTTTGATAAACGTGTAGCGCCAT
CAGTTGCTCGTAATGTTGCTAAAGCGGCAATGGAATCTGGAGTAGCTAGGATTGAAGTTGATCCGCAAGATGTGTATGAT
AAAACAATGAAACTTACAGATTTACAATAA

Upstream 100 bases:

>100_bases
CTTACTCATTCAGATTTACCCAATATTTTTACTTTTATAATGTAATGCGTTTTATCCAAGTTATTTTTTAAAAATAAATA
TTGAATTAGGGGCTGATTTC

Downstream 100 bases:

>100_bases
AAATAATTAACGTTTAATTAAATTGCTTAAAAGTTAGCAATAAAGAGTCATTAAAATGATGAAAATGAAAGTTTATGATA
AACATTCAACAGTCAAACGA

Product: NADP-dependent malic enzyme

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPCKDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIG
PEASIPVMEGKAVLFKSFAGINGVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
HGTAIVTLAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRGAIFEGRSYGMNPTKDVVAKWTN
KDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMADNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLA
FPGIFRGALEVEATHINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIEVDPQDVYD
KTMKLTDLQ

Sequences:

>Translated_409_residues
MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPCKDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIG
PEASIPVMEGKAVLFKSFAGINGVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
HGTAIVTLAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRGAIFEGRSYGMNPTKDVVAKWTN
KDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMADNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLA
FPGIFRGALEVEATHINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIEVDPQDVYD
KTMKLTDLQ
>Mature_408_residues
SLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPCKDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGP
EASIPVMEGKAVLFKSFAGINGVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQH
GTAIVTLAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRGAIFEGRSYGMNPTKDVVAKWTNK
DKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMADNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAF
PGIFRGALEVEATHINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIEVDPQDVYDK
TMKLTDLQ

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=402, Percent_Identity=27.8606965174129, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI62420880, Length=402, Percent_Identity=27.8606965174129, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI239049447, Length=402, Percent_Identity=27.8606965174129, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI4505143, Length=394, Percent_Identity=25.3807106598985, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI4505145, Length=361, Percent_Identity=26.5927977839335, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI270265879, Length=320, Percent_Identity=27.8125, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788806, Length=416, Percent_Identity=50.9615384615385, Blast_Score=396, Evalue=1e-111,
Organism=Escherichia coli, GI87081919, Length=445, Percent_Identity=26.2921348314607, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17537199, Length=415, Percent_Identity=27.710843373494, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322823, Length=315, Percent_Identity=31.4285714285714, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI281362674, Length=445, Percent_Identity=26.7415730337079, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI21356279, Length=445, Percent_Identity=26.7415730337079, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI281362672, Length=445, Percent_Identity=26.7415730337079, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24646386, Length=443, Percent_Identity=27.313769751693, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24646388, Length=443, Percent_Identity=27.313769751693, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI78707242, Length=397, Percent_Identity=25.1889168765743, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI78707238, Length=397, Percent_Identity=25.1889168765743, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI78707240, Length=386, Percent_Identity=24.3523316062176, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 44234; Mature: 44103

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPCKDIYEDKRKVYDYTIK
CCCHHHHHHHHHCCCCCEEECCCEEECCHHHEEEEECCCCCHHHHHHHHHHHEEEEEEEC
GNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGINGVPIALNTTDTEEIIKTV
CCEEEEEECCCEEEEECCCCCCCCCEEECCCHHHHHHHCCCCCEEEEEECCCHHHHHHHH
KLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQHGTAIVTLAGLVNALRVVNK
HHHCCCCCCCCHHCCCCCCCCHHHHHHHHHCCCCEEECCCCCEEEHHHHHHHHHHHHHCC
DIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRGAIFEGRSYGMNPTKDVVAKWTN
CCEEEEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCEECCCCCCCCCHHHHHHHCCC
KDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMADNPIIFAMANPNPEIIPDDAKAAG
CHHCCCHHHHHHHHHHEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
ARVVGTGRSDYPNQINNVLAFPGIFRGALEVEATHINEEMKKAAVEAIADLIDSSELNED
CEEEECCCCCCCHHHHHHECCCHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCC
YCIPGPFDKRVAPSVARNVAKAAMESGVARIEVDPQDVYDKTMKLTDLQ
CCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPCKDIYEDKRKVYDYTIK
CCHHHHHHHHHCCCCCEEECCCEEECCHHHEEEEECCCCCHHHHHHHHHHHEEEEEEEC
GNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGINGVPIALNTTDTEEIIKTV
CCEEEEEECCCEEEEECCCCCCCCCEEECCCHHHHHHHCCCCCEEEEEECCCHHHHHHHH
KLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQHGTAIVTLAGLVNALRVVNK
HHHCCCCCCCCHHCCCCCCCCHHHHHHHHHCCCCEEECCCCCEEEHHHHHHHHHHHHHCC
DIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRGAIFEGRSYGMNPTKDVVAKWTN
CCEEEEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCEECCCCCCCCCHHHHHHHCCC
KDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMADNPIIFAMANPNPEIIPDDAKAAG
CHHCCCHHHHHHHHHHEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
ARVVGTGRSDYPNQINNVLAFPGIFRGALEVEATHINEEMKKAAVEAIADLIDSSELNED
CEEEECCCCCCCHHHHHHECCCHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCC
YCIPGPFDKRVAPSVARNVAKAAMESGVARIEVDPQDVYDKTMKLTDLQ
CCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]