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The map label for this gene is lpdA [H]

Identifier: 57650475

GI number: 57650475

Start: 1598534

End: 1599955

Strand: Reverse

Name: lpdA [H]

Synonym: SACOL1563

Alternate gene names: 57650475

Gene position: 1599955-1598534 (Counterclockwise)

Preceding gene: 57650476

Following gene: 57650474

Centisome position: 56.95

GC content: 34.74

Gene sequence:

>1422_bases
ATGTCAGAGAAACAATATGATTTAGTCGTTCTCGGTGGAGGTACTGCAGGATATGTTGCTGCGATACGTGCGTCTCAATT
AGGTAAAAAAGTGGCAATAGTAGAACGACAACTATTAGGAGGTACTTGTCTCCATAAAGGTTGTATTCCTACTAAATCGC
TGCTTAAATCTGCTGAAGTATTTCAAACAGTAAAACAGGCAGCAATGTTTGGCGTTGATGTCAAAGATGCTAATGTTAAT
TTTGAAAATATGTTGGCACGAAAAGAAGACATTATTAATCAAATGTATCAAGGTGTAAAGCATTTAATGCAACACAATCA
CATTGACATTTATAATGGCACGGGACGTATTCTAGGTACATCTATATTTTCACCTCAAAGTGGCACAATTTCTGTCGAAT
ATGAAGATGGTGAATCAGACTTATTACCCAACCAATTTGTATTAATTGCAACTGGTTCTTCGCCTGCAGAGTTGCCATTC
TTATCATTTGATCATGATAAAATTTTATCTAGCGATGATATATTATCATTAAAGACGTTACCATCAAGTATTGGTATTAT
TGGTGGTGGTGTTATCGGAATGGAATTTGCATCATTGATGATAGATTTAGGTGTTGACGTAACAGTTATAGAAGCAGGTG
AAAGAATTTTACCAACTGAAAGTAAACAAGCTTCACAACTATTAAAAAAATCATTGTCAGCACGTGGTGTTAAATTCTAT
GAGGGAATAAAACTTTCTGAAAATGATATAAATGTTAACGAAGATGGTGTTACGTTTGAAATTTCCTCAGACATAATTAA
AGTAGATAAAGTGTTGCTATCGATTGGTAGAAAACCTAACACATCAGATATTGGTTTAAACAACACTAAAATAAAACTTT
CAACATCAGGACATATTTTAACGAACGAATTTCAACAAACTGAAGATAAACATATTTATGCAGCTGGTGATTGTATAGGA
AAATTACAATTGGCACATGTTGGATCAAAAGAAGGTGTTGTGGCTGTTGATCATATGTTTGAGGGGAACCCAATCCCAGT
AAACTATAACATGATGCCTAAGTGTATTTATTCACAACCTGAAATTGCTTCTATCGGTTTAAACATTGAACAAGCAAAGG
CAGAGGGAATGAAAGTTAAAAGTTTTAAAGTACCATTTAAAGCAATTGGTAAAGCAGTGATTGATAGCCATGACACAAAC
GAAGGGTATAGCGAAATGGTGATTGATCAATCAACTGAAGAAATTGTGGGTATTAATATGATTGGTCCACATGTAACAGA
ATTGATTAATGAGGCATCACTGTTACAGTTCATGAATGGCTCGGCATTAGAATTAGGACTAACAACACACGCACATCCTT
CCATCTCTGAAGTGTTGATGGAATTAGGATTGAAAGCAGAAAGTAGAGCTATTCACGTATAA

Upstream 100 bases:

>100_bases
AACTAAATAGACAATCATGTAAAATAGGTAATTAATGGACATGAAAACTATGTATTAATAAATTTTCAATGGATATATAT
CATTAGTTAGGAGTAAGAAC

Downstream 100 bases:

>100_bases
ATAGGAGGATTTAACATGATTGATTATAAATCATTAGGCCTTAGCGAAGAAGACCTAAAAGTAATATATAAATGGATGGA
TTTAGGTAGAAAAATAGATG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCLHKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVN
FENMLARKEDIINQMYQGVKHLMQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILPTESKQASQLLKKSLSARGVKFY
EGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIGRKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIG
KLQLAHVGSKEGVVAVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKAVIDSHDTN
EGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTHAHPSISEVLMELGLKAESRAIHV

Sequences:

>Translated_473_residues
MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCLHKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVN
FENMLARKEDIINQMYQGVKHLMQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILPTESKQASQLLKKSLSARGVKFY
EGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIGRKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIG
KLQLAHVGSKEGVVAVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKAVIDSHDTN
EGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTHAHPSISEVLMELGLKAESRAIHV
>Mature_472_residues
SEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCLHKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNF
ENMLARKEDIINQMYQGVKHLMQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPFL
SFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILPTESKQASQLLKKSLSARGVKFYE
GIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIGRKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGK
LQLAHVGSKEGVVAVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKAVIDSHDTNE
GYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTHAHPSISEVLMELGLKAESRAIHV

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=475, Percent_Identity=34.7368421052632, Blast_Score=263, Evalue=3e-70,
Organism=Homo sapiens, GI50301238, Length=473, Percent_Identity=28.3298097251586, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI22035672, Length=490, Percent_Identity=25.5102040816327, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI33519430, Length=498, Percent_Identity=25.7028112449799, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI33519428, Length=498, Percent_Identity=25.7028112449799, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI33519426, Length=498, Percent_Identity=25.7028112449799, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI148277065, Length=498, Percent_Identity=25.7028112449799, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI148277071, Length=498, Percent_Identity=25.7028112449799, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI291045266, Length=483, Percent_Identity=23.8095238095238, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI291045268, Length=479, Percent_Identity=22.7557411273486, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI1786307, Length=472, Percent_Identity=31.5677966101695, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI87082354, Length=469, Percent_Identity=28.5714285714286, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1789915, Length=471, Percent_Identity=26.3269639065817, Blast_Score=158, Evalue=7e-40,
Organism=Escherichia coli, GI87081717, Length=468, Percent_Identity=25.8547008547009, Blast_Score=139, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI32565766, Length=471, Percent_Identity=34.6072186836518, Blast_Score=265, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI17557007, Length=493, Percent_Identity=23.9350912778905, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71982272, Length=494, Percent_Identity=23.6842105263158, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71983429, Length=372, Percent_Identity=26.0752688172043, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=372, Percent_Identity=26.0752688172043, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321091, Length=488, Percent_Identity=35.2459016393443, Blast_Score=244, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6325166, Length=477, Percent_Identity=24.7379454926625, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6325240, Length=477, Percent_Identity=26.4150943396226, Blast_Score=143, Evalue=5e-35,
Organism=Drosophila melanogaster, GI21358499, Length=475, Percent_Identity=33.2631578947368, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI17737741, Length=499, Percent_Identity=25.250501002004, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640553, Length=506, Percent_Identity=25.098814229249, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24640549, Length=506, Percent_Identity=25.098814229249, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24640551, Length=506, Percent_Identity=25.4940711462451, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51112; Mature: 50981

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCLHKGCIPTKSLLKSAEV
CCCCCEEEEEECCCCHHHHHHHHHHHCCCEEHHHHHHHHCCHHHHCCCCCHHHHHHHHHH
FQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHLMQHNHIDIYNGTGRILGT
HHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEE
SIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPFLSFDHDKILSSDDILSLKTL
EEECCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCEEECCHHHHCCCCCEEEEECC
PSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILPTESKQASQLLKKSLSARGVKFY
CCCCCEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEE
EGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIGRKPNTSDIGLNNTKIKLSTSGHIL
CCEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEE
TNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVVAVDHMFEGNPIPVNYNMMPKCIYSQP
ECHHHCCCCCEEEEECCCCCEEEEEECCCCCCEEEEEEEECCCCEEECCCCCCCEECCCC
EIASIGLNIEQAKAEGMKVKSFKVPFKAIGKAVIDSHDTNEGYSEMVIDQSTEEIVGINM
CEEEECCCHHHHHCCCEEEEEEECCHHHHHHHHHCCCCCCCCHHHHEECCCCCCEEEEEE
IGPHVTELINEASLLQFMNGSALELGLTTHAHPSISEVLMELGLKAESRAIHV
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCEECC
>Mature Secondary Structure 
SEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCLHKGCIPTKSLLKSAEV
CCCCEEEEEECCCCHHHHHHHHHHHCCCEEHHHHHHHHCCHHHHCCCCCHHHHHHHHHH
FQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHLMQHNHIDIYNGTGRILGT
HHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEE
SIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPFLSFDHDKILSSDDILSLKTL
EEECCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCEEECCHHHHCCCCCEEEEECC
PSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILPTESKQASQLLKKSLSARGVKFY
CCCCCEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEE
EGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIGRKPNTSDIGLNNTKIKLSTSGHIL
CCEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEE
TNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVVAVDHMFEGNPIPVNYNMMPKCIYSQP
ECHHHCCCCCEEEEECCCCCEEEEEECCCCCCEEEEEEEECCCCEEECCCCCCCEECCCC
EIASIGLNIEQAKAEGMKVKSFKVPFKAIGKAVIDSHDTNEGYSEMVIDQSTEEIVGINM
CEEEECCCHHHHHCCCEEEEEEECCHHHHHHHHHCCCCCCCCHHHHEECCCCCCEEEEEE
IGPHVTELINEASLLQFMNGSALELGLTTHAHPSISEVLMELGLKAESRAIHV
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8504804 [H]