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The map label for this gene is nth [H]

Identifier: 57650407

GI number: 57650407

Start: 1531312

End: 1531971

Strand: Reverse

Name: nth [H]

Synonym: SACOL1492

Alternate gene names: 57650407

Gene position: 1531971-1531312 (Counterclockwise)

Preceding gene: 57650408

Following gene: 57650406

Centisome position: 54.53

GC content: 34.39

Gene sequence:

>660_bases
ATGGTAAGTAAGAAAAAAGCATTAGAAATGATTGATGTTATAGCAAATATGTTTCCGGATGCAGAATGTGAATTAAAACA
TGATAATCCGTTCGAATTAACTATTGCTGTATTATTGTCAGCGCAATGTACAGACGTTCTAGTAAATAGAGTAACAACTG
AGCTGTTTAAAAAATACAAAACGCCTGAAGATTATTTGGCAGTCAGTGATGAAGAACTTATGAATGATATTCGTTCTATC
GGTCTGTATCGTAACAAAGCTAAGAATATTAAGAAACTTTGTCAATCTTTGATTGATCAATTTAATGGAGAAATACCACA
AACACATAAGGAATTAGAAAGTTTAGCAGGTGTAGGACGTAAAACTGCTAATGTAGTCATGAGTGTAGCATTTGATGAAC
CTTCATTAGCTGTTGATACGCATGTAGAACGTGTTTCTAAACGCTTAGGTATTAATCGTTGGAAAGATAATGTGAGACAG
GTAGAAGACCGTTTATGTTCTGTCATACCTAGAGATAGATGGAATAGGAGCCATCATCAACTCATTTTCTTTGGAAGATA
CCACTGTTTAGCCCGAAAACCTAAATGCGATATTTGTCCACTATTAGAAGATTGTAGAGAAGGACAAAAACGTTATAAAG
CTAGTTTGAAAGAAGCGTGA

Upstream 100 bases:

>100_bases
GACGATTCTAGAAAAATAAGAGAAAAATTCAATAAACCGAAAATGACGCACACAGTTAAAACGGTACCTAAATTTGATTG
GTTAAACGGGGAGAACCTTG

Downstream 100 bases:

>100_bases
TAACATGACAAATATTACAAAAGAGGTATTTGATAATTTAGAACAAGAGATTGATTTATTTGCCAAAAATAAGACATTAG
GTTCTAGTGAAGCAAAGCCC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCTDVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSI
GLYRNKAKNIKKLCQSLIDQFNGEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKEA

Sequences:

>Translated_219_residues
MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCTDVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSI
GLYRNKAKNIKKLCQSLIDQFNGEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKEA
>Mature_219_residues
MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCTDVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSI
GLYRNKAKNIKKLCQSLIDQFNGEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKEA

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=174, Percent_Identity=32.183908045977, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1787920, Length=209, Percent_Identity=45.933014354067, Blast_Score=179, Evalue=1e-46,
Organism=Escherichia coli, GI1789331, Length=182, Percent_Identity=25.8241758241758, Blast_Score=75, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17554540, Length=171, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324530, Length=184, Percent_Identity=27.7173913043478, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319304, Length=180, Percent_Identity=27.2222222222222, Blast_Score=68, Evalue=9e-13,
Organism=Drosophila melanogaster, GI45550361, Length=178, Percent_Identity=32.0224719101124, Blast_Score=94, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 25234; Mature: 25234

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCTDVLVNRVTTELFKKYK
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFNGEIPQTHKELESLAGVGR
CCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCH
KTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQVEDRLCSVIPRDRWNRSHHQ
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCE
LIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKEA
EEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCTDVLVNRVTTELFKKYK
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFNGEIPQTHKELESLAGVGR
CCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCH
KTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQVEDRLCSVIPRDRWNRSHHQ
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCE
LIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKEA
EEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]