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The map label for this gene is cls1

Identifier: 57650320

GI number: 57650320

Start: 1357732

End: 1359213

Strand: Direct

Name: cls1

Synonym: SACOL1351

Alternate gene names: 57650320

Gene position: 1357732-1359213 (Clockwise)

Preceding gene: 57650319

Following gene: 57650321

Centisome position: 48.33

GC content: 30.97

Gene sequence:

>1482_bases
ATGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATT
GGCTTTTATTATTATCTTTTTAGAAAGAAATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCT
TACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCACGCAAATTGAATAAAAACAATGGTAAC
GTGTTAACGGATTTCGATGGACTTTTAAAACAACAAATAGAAAGCTTTGATAAAGGTAATTATGGTACTGATAACAAACA
AGTTCAAAAACATCATGATTTAGTACGTATGCTTTTGATGGATCAAGATGGTTTTTTAACTGAAAATAATAAAGTTGATC
ATTTCATTGATGGAAATGATTTATATGATCAAGTTTTAAAAGATATTAAAAATGCAAAAGAATATATCCATTTAGAGTAC
TATACTTTCGCTTTAGATGGTTTAGGTAAAAGAATTTTACATGCTTTAGAAGAAAAATTGAAACAAGGTCTAGAAGTAAA
AATATTATATGATGATGTTGGATCTAAAAATGTTAAGATGGCAAATTTTGATCATTTTAAATCGTTAGGTGGAGAAGTTG
AAGCATTTTTTGCTTCAAAATTACCGTTATTGAATTTCAGAATGAATAATAGAAATCATAGAAAAATCATCGTAATCGAT
GGTCAACTAGGTTATGTCGGAGGATTTAACATTGGTGATGAATATCTAGGATTAGGAAAATTAGGATATTGGAGAGATAC
GCATTTACGTATACAAGGGGATGCGGTTGATGCACTGCAGTTGCGATTTATTTTAGACTGGAATTCGCAAGCGCACCGTC
CACAATTTGAATATGATGTTAAGTATTTCCCTAAAAAGAACGGACCATTGGGCAATTCACCAATTCAAATAGCTGCAAGT
GGCCCGGCTAGTGACTGGCATCAAATTGAATACGGTTATACAAAAATGATTATGAGTGCAAAGAAATCTGTATATTTACA
ATCACCATATTTCATTCCGGATAATTCATATATAAATGCCATTAAAATTGCTGCTAAATCAGGTGTAGATGTACATTTAA
TGATTCCATGTAAGCCAGATCATCCATTAGTATATTGGGCGACATTTTCAAATGCCTCTGACTTATTATCAAGTGGTGTT
AAAATTTATACGTATGAAAATGGATTTATACATTCTAAAATGTGCTTAATTGATGATGAAATCGTATCAGTGGGCACAGC
AAATATGGACTTTAGAAGTTTTGAATTAAATTTTGAAGTAAATGCCTTTGTATATGATGAAAATCTTGCTAAAGATTTAA
GGGTGGCTTATGAACATGATATTACAAAATCAAAACAACTAACCAAAGAATCATATGCCAATAGACCGCTGTCTGTTAAA
TTCAAAGAATCGTTAGCAAAATTAGTTTCGCCAATTTTATAA

Upstream 100 bases:

>100_bases
AGAAGAATTCAATGCAATTTTAACTTTATCTGTGAATAAATAATATGACGTGAAAGGTGCAAAATGTTTTTTTGTGCCTT
TCTTTATAGGAGAAAAGACT

Downstream 100 bases:

>100_bases
TTTATTTGTAAGGAGTCTCGATTATAGAGGCTCTTTTTATTTTGCCCAAAATTAAAATAACGATAAATATATTATGTAAA
TCTAATAATGACATTTGTCA

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase 1

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MRFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASSTWAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGN
VLTDFDGLLKQQIESFDKGNYGTDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEY
YTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFASKLPLLNFRMNNRNHRKIIVID
GQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAVDALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAAS
GPASDWHQIEYGYTKMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNASDLLSSGV
KIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLAKDLRVAYEHDITKSKQLTKESYANRPLSVK
FKESLAKLVSPIL

Sequences:

>Translated_493_residues
MRFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASSTWAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGN
VLTDFDGLLKQQIESFDKGNYGTDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEY
YTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFASKLPLLNFRMNNRNHRKIIVID
GQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAVDALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAAS
GPASDWHQIEYGYTKMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNASDLLSSGV
KIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLAKDLRVAYEHDITKSKQLTKESYANRPLSVK
FKESLAKLVSPIL
>Mature_493_residues
MRFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASSTWAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGN
VLTDFDGLLKQQIESFDKGNYGTDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEY
YTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFASKLPLLNFRMNNRNHRKIIVID
GQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAVDALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAAS
GPASDWHQIEYGYTKMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNASDLLSSGV
KIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLAKDLRVAYEHDITKSKQLTKESYANRPLSVK
FKESLAKLVSPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains

Homologues:

Organism=Escherichia coli, GI1787502, Length=499, Percent_Identity=27.4549098196393, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1787007, Length=331, Percent_Identity=29.0030211480363, Blast_Score=139, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CLS1_STAAC (Q5HGA3)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_186204.1
- ProteinModelPortal:   Q5HGA3
- STRING:   Q5HGA3
- EnsemblBacteria:   EBSTAT00000009380
- GeneID:   3237976
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL1351
- TIGR:   SACOL1351
- eggNOG:   COG1502
- GeneTree:   EBGT00050000023642
- HOGENOM:   HBG694312
- OMA:   SKKVKMA
- ProtClustDB:   CLSK885271
- BioCyc:   SAUR93062:SACOL1351-MONOMER
- HAMAP:   MF_00190
- InterPro:   IPR022924
- InterPro:   IPR001736
- SMART:   SM00155

Pfam domain/function: PF00614 PLDc

EC number: 2.7.8.- [C]

Molecular weight: Translated: 56366; Mature: 56366

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS50035 PLD

Important sites: ACT_SITE 233-233 ACT_SITE 235-235 ACT_SITE 240-240 ACT_SITE 411-411 ACT_SITE 413-413 ACT_SITE 418-418

Signals:

None

Transmembrane regions:

HASH(0xe4a325c)-; HASH(0xe23f970)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASSTWAWLFVLFVLPLIGFI
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYGTDNKQVQKHHDLVRMLLM
HHHHHCCCCEEHEECCCCCCEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
DQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEYYTFALDGLGKRILHALEEKL
CCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHEEEEEEEEEEHHHHHHHHHHHHHHHH
KQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFASKLPLLNFRMNNRNHRKIIVID
HCCCEEEEEEECCCCCCEEECCCHHHHHCCCCEEEHHHCCCCEEEEEECCCCCCEEEEEE
GQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAVDALQLRFILDWNSQAHRPQFEYDV
CCCCEECCCCCCHHHCCCCCCCCEECCEEEEECCCCCEEEEEEEEECCCCCCCCCCEECE
KYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYTKMIMSAKKSVYLQSPYFIPDNSYINA
EECCCCCCCCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHCEEEECCEECCCCCCEEE
IKIAAKSGVDVHLMIPCKPDHPLVYWATFSNASDLLSSGVKIYTYENGFIHSKMCLIDDE
EEEEECCCCEEEEEEECCCCCCEEEEEECCCHHHHHHCCEEEEEECCCEEEEEEEEECCH
IVSVGTANMDFRSFELNFEVNAFVYDENLAKDLRVAYEHDITKSKQLTKESYANRPLSVK
HHEECCCCCCEEEEEEEEEEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEH
FKESLAKLVSPIL
HHHHHHHHHHCCH
>Mature Secondary Structure
MRFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASSTWAWLFVLFVLPLIGFI
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYGTDNKQVQKHHDLVRMLLM
HHHHHCCCCEEHEECCCCCCEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
DQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEYYTFALDGLGKRILHALEEKL
CCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHEEEEEEEEEEHHHHHHHHHHHHHHHH
KQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFASKLPLLNFRMNNRNHRKIIVID
HCCCEEEEEEECCCCCCEEECCCHHHHHCCCCEEEHHHCCCCEEEEEECCCCCCEEEEEE
GQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAVDALQLRFILDWNSQAHRPQFEYDV
CCCCEECCCCCCHHHCCCCCCCCEECCEEEEECCCCCEEEEEEEEECCCCCCCCCCEECE
KYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYTKMIMSAKKSVYLQSPYFIPDNSYINA
EECCCCCCCCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHCEEEECCEECCCCCCEEE
IKIAAKSGVDVHLMIPCKPDHPLVYWATFSNASDLLSSGVKIYTYENGFIHSKMCLIDDE
EEEEECCCCEEEEEEECCCCCCEEEEEECCCHHHHHHCCEEEEEECCCEEEEEEEEECCH
IVSVGTANMDFRSFELNFEVNAFVYDENLAKDLRVAYEHDITKSKQLTKESYANRPLSVK
HHEECCCCCCEEEEEEEEEEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEH
FKESLAKLVSPIL
HHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA