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The map label for this gene is femC

Identifier: 57650300

GI number: 57650300

Start: 1346582

End: 1347922

Strand: Direct

Name: femC

Synonym: SACOL1329

Alternate gene names: 57650300

Gene position: 1346582-1347922 (Clockwise)

Preceding gene: 57650299

Following gene: 57650301

Centisome position: 47.93

GC content: 35.2

Gene sequence:

>1341_bases
ATGCCAAAACGTACTTTCACTAAAGACGACATTCGTAAATTTGCAGAAGAGGAAAATGTAAGATATTTAAGATTACAATT
CACTGATATTTTAGGAACAATTAAAAATGTTGAAGTGCCTGTAAGCCAATTAGAAAAAGTACTTGATAACGAAATGATGT
TTGACGGTTCTTCTATCGAAGGTTTCGTACGTATCGAAGAATCAGATATGTACTTACATCCAGATTTAGATACTTGGGTA
ATCTTCCCATGGACTGCTGGACAAGGTAAAGTTGCACGTTTAATTTGTGATGTATATAAAACAGATGGAACACCATTTGA
AGGGGATCCTCGTGCAAACTTAAAACGTGTATTAAAAGAAATGGAAGATTTAGGCTTCACAGACTTTAACCTAGGGCCTG
AACCAGAATTCTTCTTGTTTAAGTTGGATGAAAAAGGGGAACCAACTTTAGAACTTAATGATGATGGTGGATATTTCGAT
TTAGCACCTACAGATTTAGGTGAAAACTGTCGTCGTGATATTGTTTTAGAATTAGAGGATATGGGCTTCGATATTGAAGC
TAGTCACCATGAAGTTGCCCCTGGTCAACATGAAATTGACTTTAAATATGCAGATGCTGTTACAGCATGTGATAATATCC
AAACATTTAAATTGGTTGTTAAAACAATCGCACGTAAACATAATTTACACGCAACATTTATGCCTAAACCATTATTCGGT
GTGAATGGTAGCGGTATGCACTTTAACGTTTCATTATTCAAAGGTAAAGAAAATGCATTCTTTGATCCAAATACTGAAAT
GGGCTTAACGGAAACTGCATATCAATTTACAGCAGGTGTGCTTAAAAATGCACGCGGATTTACTGCTGTATGTAACCCGT
TAGTAAACTCATATAAACGTTTAGTACCTGGTTATGAAGCACCATGTTATATTGCATGGAGTGGTAAAAACCGTTCACCA
TTAATCCGTGTACCATCTTCAAGAGGATTATCTACTCGTATCGAAGTACGTTCAGTAGATCCAGCTGCAAACCCATACAT
GGCGTTAGCTGCAATCTTAGAAGCTGGACTAGATGGTATTAAAAATAAATTAAAAGTTCCAGAACCAGTTAACCAAAATA
TTTACGAAATGAACCGTGAAGAACGTGAAGCAGTAGGCATTCAAGACTTACCTTCAACACTTTATACTGCATTAAAAGCA
ATGCGTGAAAATGAAGTTATTAAAAAAGCTTTAGGAAATCATATCTATAATCAATTTATTAATTCAAAATCAATTGAATG
GGATTACTACAGAACTCAAGTCTCTGAATGGGAAAGAGATCAGTACATGAAGCAATATTAA

Upstream 100 bases:

>100_bases
GATGATTGTAGATGCCACGCAAAAGCCTATTGGAGAAACTTTGCCAATAAATCGTGGTGATTTATCCCGATTTATTAAAT
AAAATTTGGAGGATTTTAAA

Downstream 100 bases:

>100_bases
TACGCTAAACAATGATGGGAAATCAGTAATATCAAGGGCTAGAGCATTTTGCTTTAGCTCTTTTTTGATTTGGAAAGATG
GGATTTCACTTGACCACTTT

Product: glutamine synthetase FemC

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQLEKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWV
IFPWTAGQGKVARLICDVYKTDGTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKLVVKTIARKHNLHATFMPKPLFG
VNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQFTAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSP
LIRVPSSRGLSTRIEVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPSTLYTALKA
MRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY

Sequences:

>Translated_446_residues
MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQLEKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWV
IFPWTAGQGKVARLICDVYKTDGTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKLVVKTIARKHNLHATFMPKPLFG
VNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQFTAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSP
LIRVPSSRGLSTRIEVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPSTLYTALKA
MRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY
>Mature_445_residues
PKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQLEKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVI
FPWTAGQGKVARLICDVYKTDGTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFDL
APTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKLVVKTIARKHNLHATFMPKPLFGV
NGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQFTAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPL
IRVPSSRGLSTRIEVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPSTLYTALKAM
RENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family

Homologues:

Organism=Homo sapiens, GI221307485, Length=453, Percent_Identity=24.9448123620309, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI1790301, Length=428, Percent_Identity=40.6542056074766, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI87081870, Length=460, Percent_Identity=27.6086956521739, Blast_Score=145, Evalue=4e-36,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): GLNA_STAAC (Q5HGC3)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_186184.1
- ProteinModelPortal:   Q5HGC3
- SMR:   Q5HGC3
- STRING:   Q5HGC3
- EnsemblBacteria:   EBSTAT00000009861
- GeneID:   3237965
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL1329
- TIGR:   SACOL1329
- eggNOG:   COG0174
- GeneTree:   EBGT00050000024288
- HOGENOM:   HBG553755
- OMA:   AMFDGSS
- ProtClustDB:   CLSK885265
- BioCyc:   SAUR93062:SACOL1329-MONOMER
- GO:   GO:0005737
- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- Gene3D:   G3DSA:3.30.590.10
- TIGRFAMs:   TIGR00653

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N; SSF54368 Gln_synt_beta

EC number: =6.3.1.2

Molecular weight: Translated: 50841; Mature: 50710

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00180 GLNA_1; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQLEKVLDNEMMFDGSSIE
CCCCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCCC
GFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTDGTPFEGDPRANLKRVLKE
EEEEEECCCEEECCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
MEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFDLAPTDLGENCRRDIVLELED
HHHCCCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCEEECCCCCCCCCCCCCEEEEEHH
MGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKLVVKTIARKHNLHATFMPKPLFG
CCCEEECCCCCCCCCCCCCCEEHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEECCCCCCC
VNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQFTAGVLKNARGFTAVCNPLVNSYKR
CCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRIEVRSVDPAANPYMALAAILEAGLDGI
HCCCCCCCEEEEECCCCCCCEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHH
KNKLKVPEPVNQNIYEMNREEREAVGIQDLPSTLYTALKAMRENEVIKKALGNHIYNQFI
HHCCCCCCCCCCHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NSKSIEWDYYRTQVSEWERDQYMKQY
CCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQLEKVLDNEMMFDGSSIE
CCCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCCC
GFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTDGTPFEGDPRANLKRVLKE
EEEEEECCCEEECCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
MEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFDLAPTDLGENCRRDIVLELED
HHHCCCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCEEECCCCCCCCCCCCCEEEEEHH
MGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKLVVKTIARKHNLHATFMPKPLFG
CCCEEECCCCCCCCCCCCCCEEHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEECCCCCCC
VNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQFTAGVLKNARGFTAVCNPLVNSYKR
CCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRIEVRSVDPAANPYMALAAILEAGLDGI
HCCCCCCCEEEEECCCCCCCEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHH
KNKLKVPEPVNQNIYEMNREEREAVGIQDLPSTLYTALKAMRENEVIKKALGNHIYNQFI
HHCCCCCCCCCCHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NSKSIEWDYYRTQVSEWERDQYMKQY
CCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA