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The map label for this gene is dpnA [H]

Identifier: 57238157

GI number: 57238157

Start: 1337677

End: 1338432

Strand: Reverse

Name: dpnA [H]

Synonym: CJE1421

Alternate gene names: 57238157

Gene position: 1338432-1337677 (Counterclockwise)

Preceding gene: 57238158

Following gene: 57238156

Centisome position: 75.28

GC content: 29.76

Gene sequence:

>756_bases
ATGTTTCACCCACAGCTTTATAACGACCACTTTCAAAATTTTAAAAGATATAATATACCAAAAGCACAGCTTGTAATAGC
TGATATTCCTTATAATTTAGGCAACAATGCTTATGCTTCATCTCCTGAATGGTATATAAATGGGGATAATAAAAATGGAG
AAAGCAAAAAAGCAAACAAGGCGTTTTTTGATACAGATAATGATTTTAGAGTTAGCGAATTTATGCACTTTTGCTCAAAA
ATGCTTATAAAAGAACCTAAAGAATGCGGTAAAAGTCCTTGCATGATTGTTTTTTGCTCTTTTGAACAACAAACAATGTT
AATTGAAGTAGCTAAAAAATATGGCTTTAATCATTATATAAATTTGGTTTTTAGAAAACAAAGCTCATCTCAAGTTTTAA
AAGCAAATATGAAAATAGTTGGAAATTGTGAATATGCTTTAATCTTATATCGTGAAAAACTTCCAAAATTTAACAATGAT
GGAAAGATGATTTATAACTGCATGGATTGGCAAAAAGATGAAGGTATTCCTAAAGTACATCCCACACAAAAGCCTGTTAA
ATTACTAGAAAGATTAATCACTATTTTTACAGATGCAGGTGATGTTGTTATAGATCCATGTGCTGGAAGTGGTAGCACTC
TTTTAGCAGCTACAAATTTAAACCGCAAAGCTTATGGCTTTGAGATTAAAAAAGACTTTTTTAAAAGTGCTAATGAAATT
ATGTTTAAACACATAGAAAGAAGCTTATTTGCTTAA

Upstream 100 bases:

>100_bases
AATCAGAATATCAAAGACTATCAGAGCGAATTTAATGAAATAATGGAAAAAATAGCCTTTTTAAAAGAGCCTAATTTATT
TAATCAAAAAGGAAGTGATG

Downstream 100 bases:

>100_bases
GTTAGAAAGGATAAAAAATGAAAGAATTCAAAGAATACATAAAAGCTAAAATAGCATTAGAAAAAGAGCTAGAAAATACG
CAAGAAATGTTAAAGCAAAC

Product: site-specific DNA-methyltransferase

Products: NA

Alternate protein names: M.DpnIIB; Adenine-specific methyltransferase DpnIIB; M.DpnII 2 [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MFHPQLYNDHFQNFKRYNIPKAQLVIADIPYNLGNNAYASSPEWYINGDNKNGESKKANKAFFDTDNDFRVSEFMHFCSK
MLIKEPKECGKSPCMIVFCSFEQQTMLIEVAKKYGFNHYINLVFRKQSSSQVLKANMKIVGNCEYALILYREKLPKFNND
GKMIYNCMDWQKDEGIPKVHPTQKPVKLLERLITIFTDAGDVVIDPCAGSGSTLLAATNLNRKAYGFEIKKDFFKSANEI
MFKHIERSLFA

Sequences:

>Translated_251_residues
MFHPQLYNDHFQNFKRYNIPKAQLVIADIPYNLGNNAYASSPEWYINGDNKNGESKKANKAFFDTDNDFRVSEFMHFCSK
MLIKEPKECGKSPCMIVFCSFEQQTMLIEVAKKYGFNHYINLVFRKQSSSQVLKANMKIVGNCEYALILYREKLPKFNND
GKMIYNCMDWQKDEGIPKVHPTQKPVKLLERLITIFTDAGDVVIDPCAGSGSTLLAATNLNRKAYGFEIKKDFFKSANEI
MFKHIERSLFA
>Mature_251_residues
MFHPQLYNDHFQNFKRYNIPKAQLVIADIPYNLGNNAYASSPEWYINGDNKNGESKKANKAFFDTDNDFRVSEFMHFCSK
MLIKEPKECGKSPCMIVFCSFEQQTMLIEVAKKYGFNHYINLVFRKQSSSQVLKANMKIVGNCEYALILYREKLPKFNND
GKMIYNCMDWQKDEGIPKVHPTQKPVKLLERLITIFTDAGDVVIDPCAGSGSTLLAATNLNRKAYGFEIKKDFFKSANEI
MFKHIERSLFA

Specific function: Essential for establishment of a previously unmethylated plasmid transformed into the cell as single stranded DNA. Recognizes the single- or double-stranded sequence GATC, causes specific methylation on A-2 on one or both strands (respectively), and prote

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=144, Percent_Identity=34.0277777777778, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 28983; Mature: 28983

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHPQLYNDHFQNFKRYNIPKAQLVIADIPYNLGNNAYASSPEWYINGDNKNGESKKANK
CCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCC
AFFDTDNDFRVSEFMHFCSKMLIKEPKECGKSPCMIVFCSFEQQTMLIEVAKKYGFNHYI
CCCCCCCCEEHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEE
NLVFRKQSSSQVLKANMKIVGNCEYALILYREKLPKFNNDGKMIYNCMDWQKDEGIPKVH
EEEEECCCCCHHHHCCCEEEECCCEEEEEEEHHCCCCCCCCCEEEEECCCCCCCCCCCCC
PTQKPVKLLERLITIFTDAGDVVIDPCAGSGSTLLAATNLNRKAYGFEIKKDFFKSANEI
CCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHH
MFKHIERSLFA
HHHHHHHHHCC
>Mature Secondary Structure
MFHPQLYNDHFQNFKRYNIPKAQLVIADIPYNLGNNAYASSPEWYINGDNKNGESKKANK
CCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCC
AFFDTDNDFRVSEFMHFCSKMLIKEPKECGKSPCMIVFCSFEQQTMLIEVAKKYGFNHYI
CCCCCCCCEEHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEE
NLVFRKQSSSQVLKANMKIVGNCEYALILYREKLPKFNNDGKMIYNCMDWQKDEGIPKVH
EEEEECCCCCHHHHCCCEEEECCCEEEEEEEHHCCCCCCCCCEEEEECCCCCCCCCCCCC
PTQKPVKLLERLITIFTDAGDVVIDPCAGSGSTLLAATNLNRKAYGFEIKKDFFKSANEI
CCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHH
MFKHIERSLFA
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3019562; 10712690; 2824782 [H]