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The map label for this gene is gatB [H]

Identifier: 57238069

GI number: 57238069

Start: 1246683

End: 1248101

Strand: Reverse

Name: gatB [H]

Synonym: CJE1331

Alternate gene names: 57238069

Gene position: 1248101-1246683 (Counterclockwise)

Preceding gene: 57238075

Following gene: 57238068

Centisome position: 70.2

GC content: 33.33

Gene sequence:

>1419_bases
ATGTTTGAAGTAGTTATAGGTCTTGAAGTTCATACTCAGTTAAATACAAAAACAAAAATCTTTTGCTCATGTGCAACTTC
TTTTGGAGAAGCTCCAAATACCAATGTTTGTCCCACTTGTTTGGCCTTACCAGGAGCTTTGCCTGTTTTAAATGAAGAGG
CTGTAAAAAAAGCTATAGCTTTTGGAAAAGCTGTCAATGCAACAATTAATAAAAAAAGTGTTTTTAATCGTAAGAATTAT
TTTTATCCAGATTTACCTAAAGCCTATCAAATTTCACAATTTGATATCCCTATAGTAGAAAAGGGTGAGCTTTTTATCAA
TGTAAAGGGTGAAAATAAACGCATAGGTATTACAAGAGCACACTTAGAAGAAGATGCAGGTAAAAATATCCACGAAAATA
ATTTTTCTAAAGTGGACTTAAATCGTGCAGGAACTCCTTTGCTTGAGATTGTCAGCGAACCTGAGCTTAGAAGTAGCGAT
GAGGCAGTAGCTTATCTTAAAAAACTTCATTCTATTATACGCTTTTTAGACATTTCAGATGCAAATATGCAAGAAGGAAG
TTTTAGATGCGATGCAAATGTTAGTATAAGACCTAAGGGCGATACAAAACTTTATACTAGAGTAGAGATTAAAAATCTTA
ATTCTTTTCGTTTTATCCAAAAAGCTATAGAATATGAGGTAAAAAGACAAAGTGAGGCTTGGGAAGATGGAACTTACGAA
CAAGAAGTAGTTCAAGAAACTAGACTTTTTGATACGACAAATTTAGTTACAAGAAGTATGCGTGGCAAAGAAGAAGCGGC
AGAATATCGCTATTTTCCTGATCCTGATTTATTGCCTGTTTTATTAAAAGATGAGTTTTTGGATATTAAAATCCCTGAAC
TTCCTGATGAGAAGAAAGCACGCTTTATAGACGAGCTTGGTATAAAAGAAAGTGATGCTGAGGTTTTAATCAGTTCTTTA
GAAATGAGTCGTTTTTTTGAAAGTCTTATTTCTCAAAATTTAAATCCTAAACTTTGTGTAAATTGGTTAAATACTGAACT
TATGGGACTTTTAAAAGGAGAATTGACTATAGAAAATTCTCCCGTAGATGCTCAAAAGCTTGGAATTTTAATTAAACGCA
TAGAAGATGGCACTATCAGTGCAAAAGCGGCTAAAGATGTTTTGGCTTTTGTATTTGAAAATACTAGCGTGGAAATTGAT
GAAGCTATAGAAAAGCTAGGACTTAAACAAGTAAGTGATGACTCAGCCATAGAAGCGGTGATTGAACAAATTTTAAATGC
AAATGCCGATAAGGTTGCAGAGTATAAAAGTGGTAAAGAGAAGCTTTTTGGATTCTTTGTAGGTCAAACCATGAAAGAAG
GCAAAGGAGCTTTTAATCCTGCAAAAGTAAATGAAATTTTAAAAACAAAGCTTGGTTGA

Upstream 100 bases:

>100_bases
TATAAATTGTCTAAGAATTATAGCATTTAAAGTTAATATTTAAAGCCTTTCTAAAAGAAAAAAAGATAAAATAAAACATT
TAAAAATTCAAGGAAATTTT

Downstream 100 bases:

>100_bases
TGCGTATAGCGGTTATTGGTGCGGGAAAATGGGGCAGTGCTTTACATCTAGCCTTAAAAGAAAATCACAATTGTTTTATC
AGTTCTTTGCATCAGCGTGA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MFEVVIGLEVHTQLNTKTKIFCSCATSFGEAPNTNVCPTCLALPGALPVLNEEAVKKAIAFGKAVNATINKKSVFNRKNY
FYPDLPKAYQISQFDIPIVEKGELFINVKGENKRIGITRAHLEEDAGKNIHENNFSKVDLNRAGTPLLEIVSEPELRSSD
EAVAYLKKLHSIIRFLDISDANMQEGSFRCDANVSIRPKGDTKLYTRVEIKNLNSFRFIQKAIEYEVKRQSEAWEDGTYE
QEVVQETRLFDTTNLVTRSMRGKEEAAEYRYFPDPDLLPVLLKDEFLDIKIPELPDEKKARFIDELGIKESDAEVLISSL
EMSRFFESLISQNLNPKLCVNWLNTELMGLLKGELTIENSPVDAQKLGILIKRIEDGTISAKAAKDVLAFVFENTSVEID
EAIEKLGLKQVSDDSAIEAVIEQILNANADKVAEYKSGKEKLFGFFVGQTMKEGKGAFNPAKVNEILKTKLG

Sequences:

>Translated_472_residues
MFEVVIGLEVHTQLNTKTKIFCSCATSFGEAPNTNVCPTCLALPGALPVLNEEAVKKAIAFGKAVNATINKKSVFNRKNY
FYPDLPKAYQISQFDIPIVEKGELFINVKGENKRIGITRAHLEEDAGKNIHENNFSKVDLNRAGTPLLEIVSEPELRSSD
EAVAYLKKLHSIIRFLDISDANMQEGSFRCDANVSIRPKGDTKLYTRVEIKNLNSFRFIQKAIEYEVKRQSEAWEDGTYE
QEVVQETRLFDTTNLVTRSMRGKEEAAEYRYFPDPDLLPVLLKDEFLDIKIPELPDEKKARFIDELGIKESDAEVLISSL
EMSRFFESLISQNLNPKLCVNWLNTELMGLLKGELTIENSPVDAQKLGILIKRIEDGTISAKAAKDVLAFVFENTSVEID
EAIEKLGLKQVSDDSAIEAVIEQILNANADKVAEYKSGKEKLFGFFVGQTMKEGKGAFNPAKVNEILKTKLG
>Mature_472_residues
MFEVVIGLEVHTQLNTKTKIFCSCATSFGEAPNTNVCPTCLALPGALPVLNEEAVKKAIAFGKAVNATINKKSVFNRKNY
FYPDLPKAYQISQFDIPIVEKGELFINVKGENKRIGITRAHLEEDAGKNIHENNFSKVDLNRAGTPLLEIVSEPELRSSD
EAVAYLKKLHSIIRFLDISDANMQEGSFRCDANVSIRPKGDTKLYTRVEIKNLNSFRFIQKAIEYEVKRQSEAWEDGTYE
QEVVQETRLFDTTNLVTRSMRGKEEAAEYRYFPDPDLLPVLLKDEFLDIKIPELPDEKKARFIDELGIKESDAEVLISSL
EMSRFFESLISQNLNPKLCVNWLNTELMGLLKGELTIENSPVDAQKLGILIKRIEDGTISAKAAKDVLAFVFENTSVEID
EAIEKLGLKQVSDDSAIEAVIEQILNANADKVAEYKSGKEKLFGFFVGQTMKEGKGAFNPAKVNEILKTKLG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=497, Percent_Identity=34.0040241448692, Blast_Score=281, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI115534094, Length=313, Percent_Identity=38.9776357827476, Blast_Score=188, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6319391, Length=510, Percent_Identity=30.7843137254902, Blast_Score=204, Evalue=2e-53,
Organism=Drosophila melanogaster, GI45550808, Length=474, Percent_Identity=32.4894514767932, Blast_Score=251, Evalue=9e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52947; Mature: 52947

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEVVIGLEVHTQLNTKTKIFCSCATSFGEAPNTNVCPTCLALPGALPVLNEEAVKKAIA
CEEEEEEEEEEEECCCCEEEEEEEHHHCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHH
FGKAVNATINKKSVFNRKNYFYPDLPKAYQISQFDIPIVEKGELFINVKGENKRIGITRA
HCCHHCCCCCHHHHHCCCCCCCCCCCCCEECCCCCCCEEECCEEEEEEECCCCEEEEEHH
HLEEDAGKNIHENNFSKVDLNRAGTPLLEIVSEPELRSSDEAVAYLKKLHSIIRFLDISD
HHHHHCCCCCCCCCCCEEECCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
ANMQEGSFRCDANVSIRPKGDTKLYTRVEIKNLNSFRFIQKAIEYEVKRQSEAWEDGTYE
CCCCCCCEEECCCEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHH
QEVVQETRLFDTTNLVTRSMRGKEEAAEYRYFPDPDLLPVLLKDEFLDIKIPELPDEKKA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEHEECCCEEEEECCCCCCHHHH
RFIDELGIKESDAEVLISSLEMSRFFESLISQNLNPKLCVNWLNTELMGLLKGELTIENS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEECCC
PVDAQKLGILIKRIEDGTISAKAAKDVLAFVFENTSVEIDEAIEKLGLKQVSDDSAIEAV
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHCCCCCCCCCHHHHHH
IEQILNANADKVAEYKSGKEKLFGFFVGQTMKEGKGAFNPAKVNEILKTKLG
HHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MFEVVIGLEVHTQLNTKTKIFCSCATSFGEAPNTNVCPTCLALPGALPVLNEEAVKKAIA
CEEEEEEEEEEEECCCCEEEEEEEHHHCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHH
FGKAVNATINKKSVFNRKNYFYPDLPKAYQISQFDIPIVEKGELFINVKGENKRIGITRA
HCCHHCCCCCHHHHHCCCCCCCCCCCCCEECCCCCCCEEECCEEEEEEECCCCEEEEEHH
HLEEDAGKNIHENNFSKVDLNRAGTPLLEIVSEPELRSSDEAVAYLKKLHSIIRFLDISD
HHHHHCCCCCCCCCCCEEECCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
ANMQEGSFRCDANVSIRPKGDTKLYTRVEIKNLNSFRFIQKAIEYEVKRQSEAWEDGTYE
CCCCCCCEEECCCEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHH
QEVVQETRLFDTTNLVTRSMRGKEEAAEYRYFPDPDLLPVLLKDEFLDIKIPELPDEKKA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEHEECCCEEEEECCCCCCHHHH
RFIDELGIKESDAEVLISSLEMSRFFESLISQNLNPKLCVNWLNTELMGLLKGELTIENS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEECCC
PVDAQKLGILIKRIEDGTISAKAAKDVLAFVFENTSVEIDEAIEKLGLKQVSDDSAIEAV
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHCCCCCCCCCHHHHHH
IEQILNANADKVAEYKSGKEKLFGFFVGQTMKEGKGAFNPAKVNEILKTKLG
HHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA