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The map label for this gene is pglF [H]

Identifier: 57238002

GI number: 57238002

Start: 1178378

End: 1180150

Strand: Reverse

Name: pglF [H]

Synonym: CJE1263

Alternate gene names: 57238002

Gene position: 1180150-1178378 (Counterclockwise)

Preceding gene: 57238003

Following gene: 57238001

Centisome position: 66.38

GC content: 30.51

Gene sequence:

>1773_bases
ATGATTTTTTATAAAAGCAAAAGATTAGCATTTTTTTTAACTTCAGATATTGTTTTAATTTTACTTAGCGTTTATCTGGC
TTTTTCTTTGAGATTTAGTGGAGATATTCCGAGTATTTTTTATCATGGTATGATGGTTTCTGCTATTATTTTGCTTGTTT
TAAAACTTTCATTTTTGTTTGTTTTTAGAATTTATAAAGTAGCTTGGAGATTTTTTTCTCTCAATGAAGCAAGAAAGATT
TTTATCGCTTTGCTTTTAGCTGAGTTTTGTTTTTTTCTTATTTTTTATTTTTTTAGTGATTTTTTTAATCCTTTTCCAAG
AAGTGCTATTGTGATAGATTTTGTTCTTTCTTATATGTTTATAGGTACTTTAAGAATTAGCAAAAGAATGCTTGTGGATT
TTAAACCTTCTAGAATGAAAGAAGAAGAAACTCCTTGTATTGTAGTAGGGGCAACTTCTAAGGCTTTGCATTTGTTAAAA
GGTGCAAAAGAAGGTTCTTTAGGGCTTTTTCCTGTAGGCGTAGTTGATGCGAGAAAAGAGCTTATAGGGACTTATTGTGA
TAAATTTATTGTAGAAGAAAAAGAAAAAATAAAATCTTATGTAGAACAAGGGGTAAAAACTGCCATTATTGCTTTAAGAC
TTGAGCAAGAAGAGCTTAAAAAACTTTTTGAAGAACTTGTAGCTTATGGTATTTGCGATGTAAAAATATTTTCTTTTACA
AGAAACGAAGCAAGAGATATCAGTATAGAAGACTTGCTTGCTAGAAAACCAAAAGATTTAGATGATAGTGCTGTGGCGGC
TTTTTTAAAAGACAAGGTAGTTTTGGTAAGTGGAGCAGGTGGAACTATAGGCAGTGAACTTTGTAAGCAATGTATTAAAT
TTGGTGCTAAGCATCTTATTATGGTTGATCATAGTGAGTATAATCTTTATAAGATCAATGATGATTTAAATTTATATAAA
GAAAAAATTACTCCTATTTTACTGAGTATTTTAGATAAGCAAAGTTTAGATGAGGTATTAAAAACTTATAAACCTGAGCT
TATTTTACATGCAGCCGCTTATAAACATGTGCCTCTTTGTGAACAAAATCCACATTCAGCAGTAATCAATAATATTTTAG
GAACTAAAATTTTATGCGACAGTGCTAAAGAAAATAAAGTAGCTAAATTTGTGATGATAAGTACAGATAAAGCAGTGCGA
CCAACAAATATTATGGGTTGCACTAAAAGAGTTTGTGAGCTTTATACTTTAAGTATGAGTGATGAAAATTTTGAAGTTGC
TTGTGTGCGTTTTGGTAATGTTTTAGGTTCTAGTGGTAGTGTGATACCGAAATTTAAAGCACAAATTGCCAATAATGAGC
CTTTAACTTTAACGCACCCTGATATAGTGCGTTATTTTATGCTTGTGGCTGAGGCAGTGCAACTTGTTTTACAAGCTGGA
GCTATCGCAAAAGGGGGAGAACTTTTTGTTTTGGATATGGGTAAGCCTGTGAAAATCATAGATTTAGCTAAAAAAATGCT
TTTACTTTCTAATCGCAATGATTTAGAAATTAAAATCACAGGCTTAAGAAAAGGTGAGAAGCTTTATGAAGAGCTTTTGA
TTGATGAAAATGATGCTAAAACGCAATATGAGAGTATTTTTGTAGCAAAGAATGAGAAGGTTGATCTTGATTGGCTTAAT
AAAGAGATAGAAAATTTACAAATATGTGAAGATATTTCAGAGGCTTTATTAAAGATTGTACCTGAATTTAAACACAATAA
AGAAGGTGTATAA

Upstream 100 bases:

>100_bases
TTTTTCCAAAAAGGAATTTGTTTGCCAAGTGGCACGGCGATGAGTAAAGATGATGTTTATGAAATTTCAAAACTGATCTT
AAAGAGCATAAAGGCTTAAA

Downstream 100 bases:

>100_bases
TGTATATAAAAGATATACAAAGATTTGAAGATAATCGCTATCGTGCTAGAGCTTATATGAGTTATATTTTAACAAGAAAT
CTGCCCAATAAACTTCCTGA

Product: general glycosylation pathway protein

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MIFYKSKRLAFFLTSDIVLILLSVYLAFSLRFSGDIPSIFYHGMMVSAIILLVLKLSFLFVFRIYKVAWRFFSLNEARKI
FIALLLAEFCFFLIFYFFSDFFNPFPRSAIVIDFVLSYMFIGTLRISKRMLVDFKPSRMKEEETPCIVVGATSKALHLLK
GAKEGSLGLFPVGVVDARKELIGTYCDKFIVEEKEKIKSYVEQGVKTAIIALRLEQEELKKLFEELVAYGICDVKIFSFT
RNEARDISIEDLLARKPKDLDDSAVAAFLKDKVVLVSGAGGTIGSELCKQCIKFGAKHLIMVDHSEYNLYKINDDLNLYK
EKITPILLSILDKQSLDEVLKTYKPELILHAAAYKHVPLCEQNPHSAVINNILGTKILCDSAKENKVAKFVMISTDKAVR
PTNIMGCTKRVCELYTLSMSDENFEVACVRFGNVLGSSGSVIPKFKAQIANNEPLTLTHPDIVRYFMLVAEAVQLVLQAG
AIAKGGELFVLDMGKPVKIIDLAKKMLLLSNRNDLEIKITGLRKGEKLYEELLIDENDAKTQYESIFVAKNEKVDLDWLN
KEIENLQICEDISEALLKIVPEFKHNKEGV

Sequences:

>Translated_590_residues
MIFYKSKRLAFFLTSDIVLILLSVYLAFSLRFSGDIPSIFYHGMMVSAIILLVLKLSFLFVFRIYKVAWRFFSLNEARKI
FIALLLAEFCFFLIFYFFSDFFNPFPRSAIVIDFVLSYMFIGTLRISKRMLVDFKPSRMKEEETPCIVVGATSKALHLLK
GAKEGSLGLFPVGVVDARKELIGTYCDKFIVEEKEKIKSYVEQGVKTAIIALRLEQEELKKLFEELVAYGICDVKIFSFT
RNEARDISIEDLLARKPKDLDDSAVAAFLKDKVVLVSGAGGTIGSELCKQCIKFGAKHLIMVDHSEYNLYKINDDLNLYK
EKITPILLSILDKQSLDEVLKTYKPELILHAAAYKHVPLCEQNPHSAVINNILGTKILCDSAKENKVAKFVMISTDKAVR
PTNIMGCTKRVCELYTLSMSDENFEVACVRFGNVLGSSGSVIPKFKAQIANNEPLTLTHPDIVRYFMLVAEAVQLVLQAG
AIAKGGELFVLDMGKPVKIIDLAKKMLLLSNRNDLEIKITGLRKGEKLYEELLIDENDAKTQYESIFVAKNEKVDLDWLN
KEIENLQICEDISEALLKIVPEFKHNKEGV
>Mature_590_residues
MIFYKSKRLAFFLTSDIVLILLSVYLAFSLRFSGDIPSIFYHGMMVSAIILLVLKLSFLFVFRIYKVAWRFFSLNEARKI
FIALLLAEFCFFLIFYFFSDFFNPFPRSAIVIDFVLSYMFIGTLRISKRMLVDFKPSRMKEEETPCIVVGATSKALHLLK
GAKEGSLGLFPVGVVDARKELIGTYCDKFIVEEKEKIKSYVEQGVKTAIIALRLEQEELKKLFEELVAYGICDVKIFSFT
RNEARDISIEDLLARKPKDLDDSAVAAFLKDKVVLVSGAGGTIGSELCKQCIKFGAKHLIMVDHSEYNLYKINDDLNLYK
EKITPILLSILDKQSLDEVLKTYKPELILHAAAYKHVPLCEQNPHSAVINNILGTKILCDSAKENKVAKFVMISTDKAVR
PTNIMGCTKRVCELYTLSMSDENFEVACVRFGNVLGSSGSVIPKFKAQIANNEPLTLTHPDIVRYFMLVAEAVQLVLQAG
AIAKGGELFVLDMGKPVKIIDLAKKMLLLSNRNDLEIKITGLRKGEKLYEELLIDENDAKTQYESIFVAKNEKVDLDWLN
KEIENLQICEDISEALLKIVPEFKHNKEGV

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=270, Percent_Identity=26.2962962962963, Blast_Score=71, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 66767; Mature: 66767

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFYKSKRLAFFLTSDIVLILLSVYLAFSLRFSGDIPSIFYHGMMVSAIILLVLKLSFLF
CCEEECCCEEEEHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
VFRIYKVAWRFFSLNEARKIFIALLLAEFCFFLIFYFFSDFFNPFPRSAIVIDFVLSYMF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
IGTLRISKRMLVDFKPSRMKEEETPCIVVGATSKALHLLKGAKEGSLGLFPVGVVDARKE
HHHHHHHHHHHEECCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCEEECCHHHHHHH
LIGTYCDKFIVEEKEKIKSYVEQGVKTAIIALRLEQEELKKLFEELVAYGICDVKIFSFT
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHEEECHHHHHHHHHHHHHHCCCCEEEEEEC
RNEARDISIEDLLARKPKDLDDSAVAAFLKDKVVLVSGAGGTIGSELCKQCIKFGAKHLI
CCCCCCCCHHHHHHCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCEEE
MVDHSEYNLYKINDDLNLYKEKITPILLSILDKQSLDEVLKTYKPELILHAAAYKHVPLC
EEECCCCEEEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHEEEHHHHCCCCCC
EQNPHSAVINNILGTKILCDSAKENKVAKFVMISTDKAVRPTNIMGCTKRVCELYTLSMS
CCCCHHHHHHHHHHHHHEECCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHEEECC
DENFEVACVRFGNVLGSSGSVIPKFKAQIANNEPLTLTHPDIVRYFMLVAEAVQLVLQAG
CCCCEEEEEEECHHHCCCCCCCHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHC
AIAKGGELFVLDMGKPVKIIDLAKKMLLLSNRNDLEIKITGLRKGEKLYEELLIDENDAK
CCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCCCC
TQYESIFVAKNEKVDLDWLNKEIENLQICEDISEALLKIVPEFKHNKEGV
CHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIFYKSKRLAFFLTSDIVLILLSVYLAFSLRFSGDIPSIFYHGMMVSAIILLVLKLSFLF
CCEEECCCEEEEHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
VFRIYKVAWRFFSLNEARKIFIALLLAEFCFFLIFYFFSDFFNPFPRSAIVIDFVLSYMF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
IGTLRISKRMLVDFKPSRMKEEETPCIVVGATSKALHLLKGAKEGSLGLFPVGVVDARKE
HHHHHHHHHHHEECCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCEEECCHHHHHHH
LIGTYCDKFIVEEKEKIKSYVEQGVKTAIIALRLEQEELKKLFEELVAYGICDVKIFSFT
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHEEECHHHHHHHHHHHHHHCCCCEEEEEEC
RNEARDISIEDLLARKPKDLDDSAVAAFLKDKVVLVSGAGGTIGSELCKQCIKFGAKHLI
CCCCCCCCHHHHHHCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCEEE
MVDHSEYNLYKINDDLNLYKEKITPILLSILDKQSLDEVLKTYKPELILHAAAYKHVPLC
EEECCCCEEEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHEEEHHHHCCCCCC
EQNPHSAVINNILGTKILCDSAKENKVAKFVMISTDKAVRPTNIMGCTKRVCELYTLSMS
CCCCHHHHHHHHHHHHHEECCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHEEECC
DENFEVACVRFGNVLGSSGSVIPKFKAQIANNEPLTLTHPDIVRYFMLVAEAVQLVLQAG
CCCCEEEEEEECHHHCCCCCCCHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHC
AIAKGGELFVLDMGKPVKIIDLAKKMLLLSNRNDLEIKITGLRKGEKLYEELLIDENDAK
CCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCCCC
TQYESIFVAKNEKVDLDWLNKEIENLQICEDISEALLKIVPEFKHNKEGV
CHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]