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The map label for this gene is leuS [H]

Identifier: 57237973

GI number: 57237973

Start: 1149175

End: 1151604

Strand: Reverse

Name: leuS [H]

Synonym: CJE1234

Alternate gene names: 57237973

Gene position: 1151604-1149175 (Counterclockwise)

Preceding gene: 57237974

Following gene: 57237972

Centisome position: 64.78

GC content: 31.07

Gene sequence:

>2430_bases
ATGGCTTATGAAGCAAGTTTAATAGAAAAAAAATGGCAAAAAATTTGGGATGAAAATGAATATTTTGAGCCAAAAGATGA
TTTAAATTTGCCTAAAAAATATATTTTATCGATGTTTCCTTATCCTAGCGGACGCATTCACATGGGGCATGTAAGAAATT
ATACTATAGGTGATGTTTTAGCAAGATATTATCGCAAGATAGGTTTTAATGTTTTACATCCTATAGGTTTTGATAGTTTT
GGTATGCCTGCTGAAAATGCAGCTATTAAACATAAAATTCATCCTAAGTCATGGACTTATGAAAATATAGCTTATATGAA
AAAAGAACTTTTTTCTTTAGGTTTTTCTTTTTCTAAAAAAAGAATGCTTGCTACTTCAGATCCACTTTATACAAAATTTG
AGCAAGAATTTTTTATTAAGATGTTTGAAAAAGGTTTGATTTACACTAAAGAAGCAAATGTAAATTGGTGTGAACAAGAT
CAAACGGTTTTGGCTAATGAGCAAGTTGAAGACGGAAAATGTTGGCGTTGTGGTCATGAAGTAGTGCAGAAAAAAATGCC
AGGTTATTATGTAAAAATCACTGCTTATGCTGAGGAACTTTTAAAAGATTTAGAAGAATTAAAAGATAAATGGCCTAATC
AAGTTTTAACTATGCAAGAAAATTGGATTGGTAAAAGCGAAGGTTTGGAATTTTCTTTAAATTTAGACGAAGAAAGCAAG
CAAAAAACCAAAGAAAGTTCTTTAGAAGTTTTTACTACAAGAGCAGATACAATTTATGGAGTTTCGTATATCGCTTTAGC
TCCAGAACATAAGATTGTTCAAAATTTGCTTTCACAAAATTTATTAAATCAAGATGTATTAAATAAAATCAAAGTTATTC
AAAATCAAAGCCCAAGAGAAAGACAAAGTTCTGAAAAAGAAGGTTATTTTTTAGGAATTTATGCCATTCATCCTTTAAGC
GGAGAAAAAATTCCTTTATGGGTGGCTAATTTTGTCTTGGCTGATTATGGTAGCGGAGCGGTTATGGCTGTTCCAGCTCA
TGATGAAAGAGATTTTGAATTTGCAACAAAATATAATCTTGCTATCAAACAAGTTATTCAAACTCAAGAAAATTTACCTT
ATACGCAAAAATCAGGAAAGTTAATCCATAGTCAAGAATTTGATAATCTTGATTGTAATGAGGCAAGATTAAAAATTATT
TCTCAGTTTGAAGCTAAAAATATAGGAAAAAGGGTTGTAAATTTTAAAATTCGTGATTGGGGTGTTTCACGCCAAAGATA
TTGGGGTGCGCCTATTCCTATGATTAAATGTCAAATTTGTGGTATAGTGCCTCAAAAACTAGAAAATTTACCTATTACCC
TACCTGAAGATGTGCAAATTACAGGTGAGGGTAATCCACTTGATAAACATCCAACTTGGAAAAATTGTATTTGTCCCAAA
TGTGGCAAAGAGGCTCAAAAAGAAAGTGATACTTTAGATACTTTTTTTGAAAGTTCTTGGTATTTTGCACGTTTTGCAAG
TGATGAGAAAACTTGGCAGGAAAAAGCTTTAGATGAAAAAAGTGTAAAGTATTGGATGAGTGTGGATCAATATATAGGTG
GTATTGAACATGCAATCTTGCATTTGCTTTATGCGAGATTTTTCCAAAAAGCTTTAAGAGACTTGGGTTATTTAACACAA
AATGAACCTTTTGATAGACTTTTAACTCAGGGTATGGTGTTAAAAGATGGAGCTAAAATGTCAAAATCAAAAGGCAATGT
TGTAGATCCTGATGAGATTATAGAAAAATACGGTGCTGATACCGCAAGACTTTTTATACTTTTTGCTGCGCCTCCGGCTA
AAGAGCTTGAATGGAATGATGATGCGGTTGAAGGGGCTTATAGATTTATTTGTAAACTCTATGATAGGGCGCAAAATGTT
AAAAAAGGTGAGCTTGTAGAATTAAAACAAGAAAATTTAAATAAAGAAGAAAAATATGCAAGATTAAAGGTATATGAAGC
TTTGAAAAAATCTTTTGAAGTTTATCATCAAAGTTTTGCTTTTAATACTTTAATTGCAGCTTGCATGGAAGCTTTAAATG
CTTTAGCGCTTTGTAAAAATGAAGCTTTGGAGCAAGAGGCTTTTTATATTATTTTAAATATTTTAGAGCCTATTATTCCT
CATGTTTGTTTTGAGCTTAGCGAGGAGCTTTTTAAATGTAAAAATTTCAAAAAATTAGAACTTAAGGAAGAGGTTTTTGT
AAAAGATACTTTAAATTTAGCTGTAAGTATCAATGGTAAGAAAAGGGCTGAATTTGAAATTTCAAGTAGTGCGAGTAAAG
AAGAAATTTTAGCTTTTGCAAAAGAAAATACCGCTAAATGGCTCGAAGGTAAAAGTATAGTTAAAGAAATTTATGTGGAA
GGAAAGCTAGTTAATCTAGTAATCAAATGA

Upstream 100 bases:

>100_bases
GCTTGAGTTTAGAAATAAAGAAATTGAAAAAGCAAAAAGAAAACAAGATAAAGAACGCAATCGTGCAATGTATGAAAAAG
GAACAGTGTAAGGAGAATTT

Downstream 100 bases:

>100_bases
AAAAGATTTTGATTTTTTGTATAGGTTTATTTTTAGGTGCTTGTGGATATGTTCCTACATCAAAAATCGCTAATAATATT
TTTGATGAGAAAGTTTATGT

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 809; Mature: 808

Protein sequence:

>809_residues
MAYEASLIEKKWQKIWDENEYFEPKDDLNLPKKYILSMFPYPSGRIHMGHVRNYTIGDVLARYYRKIGFNVLHPIGFDSF
GMPAENAAIKHKIHPKSWTYENIAYMKKELFSLGFSFSKKRMLATSDPLYTKFEQEFFIKMFEKGLIYTKEANVNWCEQD
QTVLANEQVEDGKCWRCGHEVVQKKMPGYYVKITAYAEELLKDLEELKDKWPNQVLTMQENWIGKSEGLEFSLNLDEESK
QKTKESSLEVFTTRADTIYGVSYIALAPEHKIVQNLLSQNLLNQDVLNKIKVIQNQSPRERQSSEKEGYFLGIYAIHPLS
GEKIPLWVANFVLADYGSGAVMAVPAHDERDFEFATKYNLAIKQVIQTQENLPYTQKSGKLIHSQEFDNLDCNEARLKII
SQFEAKNIGKRVVNFKIRDWGVSRQRYWGAPIPMIKCQICGIVPQKLENLPITLPEDVQITGEGNPLDKHPTWKNCICPK
CGKEAQKESDTLDTFFESSWYFARFASDEKTWQEKALDEKSVKYWMSVDQYIGGIEHAILHLLYARFFQKALRDLGYLTQ
NEPFDRLLTQGMVLKDGAKMSKSKGNVVDPDEIIEKYGADTARLFILFAAPPAKELEWNDDAVEGAYRFICKLYDRAQNV
KKGELVELKQENLNKEEKYARLKVYEALKKSFEVYHQSFAFNTLIAACMEALNALALCKNEALEQEAFYIILNILEPIIP
HVCFELSEELFKCKNFKKLELKEEVFVKDTLNLAVSINGKKRAEFEISSSASKEEILAFAKENTAKWLEGKSIVKEIYVE
GKLVNLVIK

Sequences:

>Translated_809_residues
MAYEASLIEKKWQKIWDENEYFEPKDDLNLPKKYILSMFPYPSGRIHMGHVRNYTIGDVLARYYRKIGFNVLHPIGFDSF
GMPAENAAIKHKIHPKSWTYENIAYMKKELFSLGFSFSKKRMLATSDPLYTKFEQEFFIKMFEKGLIYTKEANVNWCEQD
QTVLANEQVEDGKCWRCGHEVVQKKMPGYYVKITAYAEELLKDLEELKDKWPNQVLTMQENWIGKSEGLEFSLNLDEESK
QKTKESSLEVFTTRADTIYGVSYIALAPEHKIVQNLLSQNLLNQDVLNKIKVIQNQSPRERQSSEKEGYFLGIYAIHPLS
GEKIPLWVANFVLADYGSGAVMAVPAHDERDFEFATKYNLAIKQVIQTQENLPYTQKSGKLIHSQEFDNLDCNEARLKII
SQFEAKNIGKRVVNFKIRDWGVSRQRYWGAPIPMIKCQICGIVPQKLENLPITLPEDVQITGEGNPLDKHPTWKNCICPK
CGKEAQKESDTLDTFFESSWYFARFASDEKTWQEKALDEKSVKYWMSVDQYIGGIEHAILHLLYARFFQKALRDLGYLTQ
NEPFDRLLTQGMVLKDGAKMSKSKGNVVDPDEIIEKYGADTARLFILFAAPPAKELEWNDDAVEGAYRFICKLYDRAQNV
KKGELVELKQENLNKEEKYARLKVYEALKKSFEVYHQSFAFNTLIAACMEALNALALCKNEALEQEAFYIILNILEPIIP
HVCFELSEELFKCKNFKKLELKEEVFVKDTLNLAVSINGKKRAEFEISSSASKEEILAFAKENTAKWLEGKSIVKEIYVE
GKLVNLVIK
>Mature_808_residues
AYEASLIEKKWQKIWDENEYFEPKDDLNLPKKYILSMFPYPSGRIHMGHVRNYTIGDVLARYYRKIGFNVLHPIGFDSFG
MPAENAAIKHKIHPKSWTYENIAYMKKELFSLGFSFSKKRMLATSDPLYTKFEQEFFIKMFEKGLIYTKEANVNWCEQDQ
TVLANEQVEDGKCWRCGHEVVQKKMPGYYVKITAYAEELLKDLEELKDKWPNQVLTMQENWIGKSEGLEFSLNLDEESKQ
KTKESSLEVFTTRADTIYGVSYIALAPEHKIVQNLLSQNLLNQDVLNKIKVIQNQSPRERQSSEKEGYFLGIYAIHPLSG
EKIPLWVANFVLADYGSGAVMAVPAHDERDFEFATKYNLAIKQVIQTQENLPYTQKSGKLIHSQEFDNLDCNEARLKIIS
QFEAKNIGKRVVNFKIRDWGVSRQRYWGAPIPMIKCQICGIVPQKLENLPITLPEDVQITGEGNPLDKHPTWKNCICPKC
GKEAQKESDTLDTFFESSWYFARFASDEKTWQEKALDEKSVKYWMSVDQYIGGIEHAILHLLYARFFQKALRDLGYLTQN
EPFDRLLTQGMVLKDGAKMSKSKGNVVDPDEIIEKYGADTARLFILFAAPPAKELEWNDDAVEGAYRFICKLYDRAQNVK
KGELVELKQENLNKEEKYARLKVYEALKKSFEVYHQSFAFNTLIAACMEALNALALCKNEALEQEAFYIILNILEPIIPH
VCFELSEELFKCKNFKKLELKEEVFVKDTLNLAVSINGKKRAEFEISSSASKEEILAFAKENTAKWLEGKSIVKEIYVEG
KLVNLVIK

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=874, Percent_Identity=32.9519450800915, Blast_Score=465, Evalue=1e-131,
Organism=Homo sapiens, GI94721239, Length=390, Percent_Identity=26.4102564102564, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI94721241, Length=390, Percent_Identity=26.4102564102564, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI1786861, Length=868, Percent_Identity=43.5483870967742, Blast_Score=717, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=227, Percent_Identity=26.431718061674, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71997510, Length=774, Percent_Identity=30.2325581395349, Blast_Score=298, Evalue=9e-81,
Organism=Caenorhabditis elegans, GI71997517, Length=774, Percent_Identity=30.2325581395349, Blast_Score=298, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI212645227, Length=343, Percent_Identity=29.4460641399417, Blast_Score=100, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323414, Length=782, Percent_Identity=37.8516624040921, Blast_Score=483, Evalue=1e-137,
Organism=Saccharomyces cerevisiae, GI6321531, Length=431, Percent_Identity=23.2018561484919, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21355409, Length=866, Percent_Identity=30.8314087759815, Blast_Score=376, Evalue=1e-104,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR009008 [H]

Pfam domain/function: PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 93453; Mature: 93322

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYEASLIEKKWQKIWDENEYFEPKDDLNLPKKYILSMFPYPSGRIHMGHVRNYTIGDVL
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEECCCCCCCHHHHH
ARYYRKIGFNVLHPIGFDSFGMPAENAAIKHKIHPKSWTYENIAYMKKELFSLGFSFSKK
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCC
RMLATSDPLYTKFEQEFFIKMFEKGLIYTKEANVNWCEQDQTVLANEQVEDGKCWRCGHE
CEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEECCCCCCCCCHHHHHHH
VVQKKMPGYYVKITAYAEELLKDLEELKDKWPNQVLTMQENWIGKSEGLEFSLNLDEESK
HHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCEEEECCCHHHH
QKTKESSLEVFTTRADTIYGVSYIALAPEHKIVQNLLSQNLLNQDVLNKIKVIQNQSPRE
HHHHHCCCEEEEECCCCEECCEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHH
RQSSEKEGYFLGIYAIHPLSGEKIPLWVANFVLADYGSGAVMAVPAHDERDFEFATKYNL
HHCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHH
AIKQVIQTQENLPYTQKSGKLIHSQEFDNLDCNEARLKIISQFEAKNIGKRVVNFKIRDW
HHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECC
GVSRQRYWGAPIPMIKCQICGIVPQKLENLPITLPEDVQITGEGNPLDKHPTWKNCICPK
CCCCHHCCCCCCCHHHHHHHCCCHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
CGKEAQKESDTLDTFFESSWYFARFASDEKTWQEKALDEKSVKYWMSVDQYIGGIEHAIL
CCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHH
HLLYARFFQKALRDLGYLTQNEPFDRLLTQGMVLKDGAKMSKSKGNVVDPDEIIEKYGAD
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCEEECCCHHCCCCCCCCCHHHHHHHHCCC
TARLFILFAAPPAKELEWNDDAVEGAYRFICKLYDRAQNVKKGELVELKQENLNKEEKYA
CEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHH
RLKVYEALKKSFEVYHQSFAFNTLIAACMEALNALALCKNEALEQEAFYIILNILEPIIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HVCFELSEELFKCKNFKKLELKEEVFVKDTLNLAVSINGKKRAEFEISSSASKEEILAFA
HHHHHHHHHHHHHCCCCCCCHHHHHHHEEEEEEEEEECCCCCEEEEECCCCCHHHHHHHH
KENTAKWLEGKSIVKEIYVEGKLVNLVIK
HHCHHHHHHHHHHHHHHHHCCEEEEEEEC
>Mature Secondary Structure 
AYEASLIEKKWQKIWDENEYFEPKDDLNLPKKYILSMFPYPSGRIHMGHVRNYTIGDVL
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEECCCCCCCHHHHH
ARYYRKIGFNVLHPIGFDSFGMPAENAAIKHKIHPKSWTYENIAYMKKELFSLGFSFSKK
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCC
RMLATSDPLYTKFEQEFFIKMFEKGLIYTKEANVNWCEQDQTVLANEQVEDGKCWRCGHE
CEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEECCCCCCCCCHHHHHHH
VVQKKMPGYYVKITAYAEELLKDLEELKDKWPNQVLTMQENWIGKSEGLEFSLNLDEESK
HHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCEEEECCCHHHH
QKTKESSLEVFTTRADTIYGVSYIALAPEHKIVQNLLSQNLLNQDVLNKIKVIQNQSPRE
HHHHHCCCEEEEECCCCEECCEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHH
RQSSEKEGYFLGIYAIHPLSGEKIPLWVANFVLADYGSGAVMAVPAHDERDFEFATKYNL
HHCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHH
AIKQVIQTQENLPYTQKSGKLIHSQEFDNLDCNEARLKIISQFEAKNIGKRVVNFKIRDW
HHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECC
GVSRQRYWGAPIPMIKCQICGIVPQKLENLPITLPEDVQITGEGNPLDKHPTWKNCICPK
CCCCHHCCCCCCCHHHHHHHCCCHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
CGKEAQKESDTLDTFFESSWYFARFASDEKTWQEKALDEKSVKYWMSVDQYIGGIEHAIL
CCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHH
HLLYARFFQKALRDLGYLTQNEPFDRLLTQGMVLKDGAKMSKSKGNVVDPDEIIEKYGAD
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCEEECCCHHCCCCCCCCCHHHHHHHHCCC
TARLFILFAAPPAKELEWNDDAVEGAYRFICKLYDRAQNVKKGELVELKQENLNKEEKYA
CEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHH
RLKVYEALKKSFEVYHQSFAFNTLIAACMEALNALALCKNEALEQEAFYIILNILEPIIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HVCFELSEELFKCKNFKKLELKEEVFVKDTLNLAVSINGKKRAEFEISSSASKEEILAFA
HHHHHHHHHHHHHCCCCCCCHHHHHHHEEEEEEEEEECCCCCEEEEECCCCCHHHHHHHH
KENTAKWLEGKSIVKEIYVEGKLVNLVIK
HHCHHHHHHHHHHHHHHHHCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA