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The map label for this gene is folC [H]

Identifier: 57237970

GI number: 57237970

Start: 1146135

End: 1147307

Strand: Reverse

Name: folC [H]

Synonym: CJE1231

Alternate gene names: 57237970

Gene position: 1147307-1146135 (Counterclockwise)

Preceding gene: 57237971

Following gene: 57237969

Centisome position: 64.53

GC content: 26.51

Gene sequence:

>1173_bases
ATGAACAATATAATGAAAGTTGAGAATTTTTTAGCTCAAAAAAGTATAAATTACGATAAAATTGATCGTTTTTTGATGTT
TAGAATGTATGAGAAATATAAGAAATATTTTAAAAATATTCCCATAATTCAACTTATAGGCACAAATGGTAAAGGTAGCA
CGGGTAGATATTTAACCCAACTTTTAGAAAATTTAAATTATAAAATAGGGCATTATACTAGTCCGCACATTTTCAGTTTT
AATGAAAGATTTTATCTTGATGGTAAGATTGCAAATGATGAAGAATTAGAACAAGCTCATATAAGACTTGAAGAAATTTT
TAAACAAGACTTGCAAAAATTAAGTTATTTCGAATATGCTACTTTTTTAGCTATGATTTTATTTCAAAAATGTGATTTTA
TTGTTTTAGAAGCAGGAGTGGGTGGAGAATATGATGCTACTTCTGTTTTTGAAAGAAGAATGAATATTTTTACAAGAATT
GGATTTGACCATATTCAAATTTTGGGCAATTCTTTAGAAGATATTGCAAGAACCAAGCTTAAAGTTATGGCGCCTATAGC
ACTTATAAGTGATGAGCAAGAGCAAAATGTTTTAAATTTAGCAAAAAAAATTGCTTTTTTAAAAAAAGCTAATTTGCAAG
TTTCTTCCTTAAATCCTTTTTTGAAAGAAAAATTTGAGATTTATTGTGAAAAGTTTGTTTTACCTTGTTTTTTAAAGCAT
AATTTAAAGCTTGCATTAAAGGCTTGTGAAATTTTAACTTCACAAGAAAAAACCTTAGAAGCTTTAAAAAAGTTACAAGA
GTTAAATTTACAAGGAAGATGCCAAGAAATTTCTCCAAATTTTTTTGTTGATGTGGGTCATAATCCCATGGCTGCTAAGG
CTATGCTTGATAAATTTCAAGGAGAAAAAATAAATCTTATTTATAATGCTTATTTGGATAAGGATATTTTTCAAATTTTA
AATACATTAAAGCCTATTATTGATACAATACAAATATATAAATATAAAAGTGCAGAACGAAAGCTGGCCGATGATGAAAT
TTATAGTATTGCATCAAAGCTAGGTATACAATGCAAAGAATTTGTAAAATTAGAAGAGAATAAAAAAAATCTAGTTTTTG
GTTCTTTTATGCTGGTAGAAAATTTTTTAAAGGAGTGGTGTGGTAAAAAATAA

Upstream 100 bases:

>100_bases
GATATTATAACTCCTAAAATTCACGTGGCTTATCTTGATAAGCAAAGTAAGCCAAGTATTAATATTTTAGATGAGTTAAT
AAAGCAAATTGCAGCGGTTA

Downstream 100 bases:

>100_bases
ATTCACTATTACCATTACAGATATTAACGGTTCAAGACATTTTTATTTAAATCAAATAATCAAAAAAATAGTTTTTTATA
CTATTGCTTTTATTGTTTTA

Product: folC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MNNIMKVENFLAQKSINYDKIDRFLMFRMYEKYKKYFKNIPIIQLIGTNGKGSTGRYLTQLLENLNYKIGHYTSPHIFSF
NERFYLDGKIANDEELEQAHIRLEEIFKQDLQKLSYFEYATFLAMILFQKCDFIVLEAGVGGEYDATSVFERRMNIFTRI
GFDHIQILGNSLEDIARTKLKVMAPIALISDEQEQNVLNLAKKIAFLKKANLQVSSLNPFLKEKFEIYCEKFVLPCFLKH
NLKLALKACEILTSQEKTLEALKKLQELNLQGRCQEISPNFFVDVGHNPMAAKAMLDKFQGEKINLIYNAYLDKDIFQIL
NTLKPIIDTIQIYKYKSAERKLADDEIYSIASKLGIQCKEFVKLEENKKNLVFGSFMLVENFLKEWCGKK

Sequences:

>Translated_390_residues
MNNIMKVENFLAQKSINYDKIDRFLMFRMYEKYKKYFKNIPIIQLIGTNGKGSTGRYLTQLLENLNYKIGHYTSPHIFSF
NERFYLDGKIANDEELEQAHIRLEEIFKQDLQKLSYFEYATFLAMILFQKCDFIVLEAGVGGEYDATSVFERRMNIFTRI
GFDHIQILGNSLEDIARTKLKVMAPIALISDEQEQNVLNLAKKIAFLKKANLQVSSLNPFLKEKFEIYCEKFVLPCFLKH
NLKLALKACEILTSQEKTLEALKKLQELNLQGRCQEISPNFFVDVGHNPMAAKAMLDKFQGEKINLIYNAYLDKDIFQIL
NTLKPIIDTIQIYKYKSAERKLADDEIYSIASKLGIQCKEFVKLEENKKNLVFGSFMLVENFLKEWCGKK
>Mature_390_residues
MNNIMKVENFLAQKSINYDKIDRFLMFRMYEKYKKYFKNIPIIQLIGTNGKGSTGRYLTQLLENLNYKIGHYTSPHIFSF
NERFYLDGKIANDEELEQAHIRLEEIFKQDLQKLSYFEYATFLAMILFQKCDFIVLEAGVGGEYDATSVFERRMNIFTRI
GFDHIQILGNSLEDIARTKLKVMAPIALISDEQEQNVLNLAKKIAFLKKANLQVSSLNPFLKEKFEIYCEKFVLPCFLKH
NLKLALKACEILTSQEKTLEALKKLQELNLQGRCQEISPNFFVDVGHNPMAAKAMLDKFQGEKINLIYNAYLDKDIFQIL
NTLKPIIDTIQIYKYKSAERKLADDEIYSIASKLGIQCKEFVKLEENKKNLVFGSFMLVENFLKEWCGKK

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=210, Percent_Identity=27.1428571428571, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI66932984, Length=210, Percent_Identity=27.1428571428571, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1788654, Length=307, Percent_Identity=30.2931596091205, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17553148, Length=146, Percent_Identity=33.5616438356164, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71984923, Length=146, Percent_Identity=33.5616438356164, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17553150, Length=146, Percent_Identity=33.5616438356164, Blast_Score=92, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323760, Length=330, Percent_Identity=25.1515151515152, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324815, Length=316, Percent_Identity=27.5316455696203, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24641571, Length=148, Percent_Identity=33.1081081081081, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24581568, Length=159, Percent_Identity=32.7044025157233, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 45473; Mature: 45473

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIMKVENFLAQKSINYDKIDRFLMFRMYEKYKKYFKNIPIIQLIGTNGKGSTGRYLTQ
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
LLENLNYKIGHYTSPHIFSFNERFYLDGKIANDEELEQAHIRLEEIFKQDLQKLSYFEYA
HHHHCCCCCCCCCCCCEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFLAMILFQKCDFIVLEAGVGGEYDATSVFERRMNIFTRIGFDHIQILGNSLEDIARTKL
HHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHH
KVMAPIALISDEQEQNVLNLAKKIAFLKKANLQVSSLNPFLKEKFEIYCEKFVLPCFLKH
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHC
NLKLALKACEILTSQEKTLEALKKLQELNLQGRCQEISPNFFVDVGHNPMAAKAMLDKFQ
CHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHC
GEKINLIYNAYLDKDIFQILNTLKPIIDTIQIYKYKSAERKLADDEIYSIASKLGIQCKE
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHCCCHHH
FVKLEENKKNLVFGSFMLVENFLKEWCGKK
HHHHHCCCCCEEEHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNNIMKVENFLAQKSINYDKIDRFLMFRMYEKYKKYFKNIPIIQLIGTNGKGSTGRYLTQ
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
LLENLNYKIGHYTSPHIFSFNERFYLDGKIANDEELEQAHIRLEEIFKQDLQKLSYFEYA
HHHHCCCCCCCCCCCCEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFLAMILFQKCDFIVLEAGVGGEYDATSVFERRMNIFTRIGFDHIQILGNSLEDIARTKL
HHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHH
KVMAPIALISDEQEQNVLNLAKKIAFLKKANLQVSSLNPFLKEKFEIYCEKFVLPCFLKH
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHC
NLKLALKACEILTSQEKTLEALKKLQELNLQGRCQEISPNFFVDVGHNPMAAKAMLDKFQ
CHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHC
GEKINLIYNAYLDKDIFQILNTLKPIIDTIQIYKYKSAERKLADDEIYSIASKLGIQCKE
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHCCCHHH
FVKLEENKKNLVFGSFMLVENFLKEWCGKK
HHHHHCCCCCEEEHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]