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The map label for this gene is gatA [H]

Identifier: 57237944

GI number: 57237944

Start: 1121665

End: 1123026

Strand: Reverse

Name: gatA [H]

Synonym: CJE1202

Alternate gene names: 57237944

Gene position: 1123026-1121665 (Counterclockwise)

Preceding gene: 57237945

Following gene: 57237943

Centisome position: 63.17

GC content: 34.43

Gene sequence:

>1362_bases
ATGATAACTTTAAAAGAAGCTTTAAAATACTCCAAAGAAGAACTTGAAAATTTAAAAAAAGAACTCAATGAAAAAGCAAA
AAAAGAAAAAAAAATAGGTGCTTATATCGAACAATTTTTAGATAAAGACTTAAGTGTTTCAGGTGAAGGTGTGCCTGTAG
CTATAAAAGATAATATCAGCGTAAAGGGCTGGGAGCTTACTAGTGCGAGTAAGATTTTACAAGGTTATATCGCCCCTTAT
GATGCAAGTGTGATTGTTAATTTAAAAGCTAATGGTTTTTCTCCTTTTGGGCGTTGCAATATGGATGAATTTGCTATGGG
AAGTTCAACAGCGAGTTCTTATTATGGCAAAACTTTAAATCCTTTAAATTTTGAGCGTGTGCCAGGTGGTTCAAGTGGCG
GAAGTGCTGCTGCGGTAGCGGGTGGCTTGGCTTTGGCAAGTTTGGGTTCTGATACAGGAGGATCTGTACGTCAGCCTGCA
GCTTTTTGTGGTTGCGTAGGATTTAAACCGAGTTATGGAAGAGTGAGTCGCTATGGCTTAGCTTCTTATTCTTCAAGTTT
GGATCAAATCGGGGTTTTAACGCAAAATGTAGAAGATGCGGCAATTTTATATGATGCGATCGCAGGATATGACAAAATGG
ATAGCACGAGTGCAAATATCGAGTTTATCAAAACCGCTCCAAATTTAAATGCAAATAAAAAATTAAAAATTGCAGTGATT
GAAAATTATGTCAATGATGCAGATAGTGAAGTAAAAAATGCACTTTTAAAAACCATAGATATGTTAAAAGCTAATGGACA
TGAGATTGTATATAAAAATTTGCTTGATTCTAAGTTTGATATTGCGGCGTATTATATCATCGCAACAGCTGAAGCAAGTA
CAAATTTAAGTCGTTATGATGGGGTGCGTTATGGCAAGCGTTCTGAAAATATCCAAAATTTAAAAGAAATGTATGTAAAT
ACACGCAGCGAAGGTTTTGGAGAAGAAGTAAAAAGAAGAATTTTGTTAGGAACTTTTGTTTTAAGCAGTGGATATTATGA
TGCGTATTATATTAAAGCACAAAAAGCTAGAGCTTTTATTAAGGCAAAATATGAAGAAATTTTACAAGATTGTGATCTTA
TTTTTATGCCTGTAACGCCTACAACGGCTTTTAAATTTGATACTCAAAAAAGCCCTATACAAACTTATTTAGAAGATGTT
TATACTATCTCTGTAAATTTAGCAGGGCTTGGAGGCATTAGTGTGCCTGTTGCAAAAGATAAAGAAGGGCTTAATATATC
AGCACAACTGATTTGCAAAGCTTATGATGAACAAACCTTGTTAGACGGGGCTTTAAGTTTAGAACAAATGATTAAAAATT
AA

Upstream 100 bases:

>100_bases
AAAATCCTATTCCAAATAGCATTATCATCACAACTGTAGCGGTGGTTTTGGCTTTTAGTGCTTTAGCAGTATTTTTGATC
AAAAAAACAAAGGAAAATAA

Downstream 100 bases:

>100_bases
GGATGAAAGATGAAAATTGTAAAAAGAGCTTTAACTTTTGAAGATGTATTATTGCGTCCTGGGTATTCTGAAGTTCTACC
TAAGGAAGTAAAAATTCATA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MITLKEALKYSKEELENLKKELNEKAKKEKKIGAYIEQFLDKDLSVSGEGVPVAIKDNISVKGWELTSASKILQGYIAPY
DASVIVNLKANGFSPFGRCNMDEFAMGSSTASSYYGKTLNPLNFERVPGGSSGGSAAAVAGGLALASLGSDTGGSVRQPA
AFCGCVGFKPSYGRVSRYGLASYSSSLDQIGVLTQNVEDAAILYDAIAGYDKMDSTSANIEFIKTAPNLNANKKLKIAVI
ENYVNDADSEVKNALLKTIDMLKANGHEIVYKNLLDSKFDIAAYYIIATAEASTNLSRYDGVRYGKRSENIQNLKEMYVN
TRSEGFGEEVKRRILLGTFVLSSGYYDAYYIKAQKARAFIKAKYEEILQDCDLIFMPVTPTTAFKFDTQKSPIQTYLEDV
YTISVNLAGLGGISVPVAKDKEGLNISAQLICKAYDEQTLLDGALSLEQMIKN

Sequences:

>Translated_453_residues
MITLKEALKYSKEELENLKKELNEKAKKEKKIGAYIEQFLDKDLSVSGEGVPVAIKDNISVKGWELTSASKILQGYIAPY
DASVIVNLKANGFSPFGRCNMDEFAMGSSTASSYYGKTLNPLNFERVPGGSSGGSAAAVAGGLALASLGSDTGGSVRQPA
AFCGCVGFKPSYGRVSRYGLASYSSSLDQIGVLTQNVEDAAILYDAIAGYDKMDSTSANIEFIKTAPNLNANKKLKIAVI
ENYVNDADSEVKNALLKTIDMLKANGHEIVYKNLLDSKFDIAAYYIIATAEASTNLSRYDGVRYGKRSENIQNLKEMYVN
TRSEGFGEEVKRRILLGTFVLSSGYYDAYYIKAQKARAFIKAKYEEILQDCDLIFMPVTPTTAFKFDTQKSPIQTYLEDV
YTISVNLAGLGGISVPVAKDKEGLNISAQLICKAYDEQTLLDGALSLEQMIKN
>Mature_453_residues
MITLKEALKYSKEELENLKKELNEKAKKEKKIGAYIEQFLDKDLSVSGEGVPVAIKDNISVKGWELTSASKILQGYIAPY
DASVIVNLKANGFSPFGRCNMDEFAMGSSTASSYYGKTLNPLNFERVPGGSSGGSAAAVAGGLALASLGSDTGGSVRQPA
AFCGCVGFKPSYGRVSRYGLASYSSSLDQIGVLTQNVEDAAILYDAIAGYDKMDSTSANIEFIKTAPNLNANKKLKIAVI
ENYVNDADSEVKNALLKTIDMLKANGHEIVYKNLLDSKFDIAAYYIIATAEASTNLSRYDGVRYGKRSENIQNLKEMYVN
TRSEGFGEEVKRRILLGTFVLSSGYYDAYYIKAQKARAFIKAKYEEILQDCDLIFMPVTPTTAFKFDTQKSPIQTYLEDV
YTISVNLAGLGGISVPVAKDKEGLNISAQLICKAYDEQTLLDGALSLEQMIKN

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=431, Percent_Identity=36.6589327146172, Blast_Score=273, Evalue=4e-73,
Organism=Homo sapiens, GI195972892, Length=419, Percent_Identity=23.8663484486874, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI166795287, Length=229, Percent_Identity=30.5676855895196, Blast_Score=79, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=411, Percent_Identity=36.0097323600973, Blast_Score=241, Evalue=6e-64,
Organism=Caenorhabditis elegans, GI17556264, Length=247, Percent_Identity=33.6032388663968, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI71990152, Length=234, Percent_Identity=36.7521367521368, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17537465, Length=413, Percent_Identity=23.9709443099274, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=270, Percent_Identity=26.2962962962963, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=250, Percent_Identity=30, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=250, Percent_Identity=30, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=274, Percent_Identity=25.5474452554745, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=418, Percent_Identity=35.8851674641148, Blast_Score=224, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6319685, Length=399, Percent_Identity=27.5689223057644, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648113, Length=425, Percent_Identity=36, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24644968, Length=414, Percent_Identity=26.8115942028986, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24652985, Length=456, Percent_Identity=24.780701754386, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI19922090, Length=456, Percent_Identity=24.780701754386, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24652981, Length=456, Percent_Identity=24.780701754386, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24652983, Length=456, Percent_Identity=24.780701754386, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI45550774, Length=204, Percent_Identity=30.8823529411765, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648435, Length=204, Percent_Identity=30.8823529411765, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648437, Length=204, Percent_Identity=30.8823529411765, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648441, Length=204, Percent_Identity=30.8823529411765, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24648439, Length=204, Percent_Identity=30.8823529411765, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI161078093, Length=413, Percent_Identity=26.1501210653753, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356731, Length=178, Percent_Identity=30.3370786516854, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49357; Mature: 49357

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLKEALKYSKEELENLKKELNEKAKKEKKIGAYIEQFLDKDLSVSGEGVPVAIKDNIS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCE
VKGWELTSASKILQGYIAPYDASVIVNLKANGFSPFGRCNMDEFAMGSSTASSYYGKTLN
EEEEECCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHCCCCCHHHHCCCCCC
PLNFERVPGGSSGGSAAAVAGGLALASLGSDTGGSVRQPAAFCGCVGFKPSYGRVSRYGL
CCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHH
ASYSSSLDQIGVLTQNVEDAAILYDAIAGYDKMDSTSANIEFIKTAPNLNANKKLKIAVI
HHHHCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEE
ENYVNDADSEVKNALLKTIDMLKANGHEIVYKNLLDSKFDIAAYYIIATAEASTNLSRYD
HHHHCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCEEEEEEEEECCCCCCHHHHC
GVRYGKRSENIQNLKEMYVNTRSEGFGEEVKRRILLGTFVLSSGYYDAYYIKAQKARAFI
CCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHH
KAKYEEILQDCDLIFMPVTPTTAFKFDTQKSPIQTYLEDVYTISVNLAGLGGISVPVAKD
HHHHHHHHHCCCEEEEECCCCCEEEECCCCCHHHHHHHHHEEEEEEEEECCCCCCCCCCC
KEGLNISAQLICKAYDEQTLLDGALSLEQMIKN
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MITLKEALKYSKEELENLKKELNEKAKKEKKIGAYIEQFLDKDLSVSGEGVPVAIKDNIS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCE
VKGWELTSASKILQGYIAPYDASVIVNLKANGFSPFGRCNMDEFAMGSSTASSYYGKTLN
EEEEECCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHCCCCCHHHHCCCCCC
PLNFERVPGGSSGGSAAAVAGGLALASLGSDTGGSVRQPAAFCGCVGFKPSYGRVSRYGL
CCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHH
ASYSSSLDQIGVLTQNVEDAAILYDAIAGYDKMDSTSANIEFIKTAPNLNANKKLKIAVI
HHHHCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEE
ENYVNDADSEVKNALLKTIDMLKANGHEIVYKNLLDSKFDIAAYYIIATAEASTNLSRYD
HHHHCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCEEEEEEEEECCCCCCHHHHC
GVRYGKRSENIQNLKEMYVNTRSEGFGEEVKRRILLGTFVLSSGYYDAYYIKAQKARAFI
CCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHH
KAKYEEILQDCDLIFMPVTPTTAFKFDTQKSPIQTYLEDVYTISVNLAGLGGISVPVAKD
HHHHHHHHHCCCEEEEECCCCCEEEECCCCCHHHHHHHHHEEEEEEEEECCCCCCCCCCC
KEGLNISAQLICKAYDEQTLLDGALSLEQMIKN
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA