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The map label for this gene is hipO [H]

Identifier: 57237812

GI number: 57237812

Start: 996676

End: 997827

Strand: Reverse

Name: hipO [H]

Synonym: CJE1067

Alternate gene names: 57237812

Gene position: 997827-996676 (Counterclockwise)

Preceding gene: 57237813

Following gene: 57237809

Centisome position: 56.13

GC content: 33.07

Gene sequence:

>1152_bases
ATGAATTTAATTCCAGAAATACTAGACTTACAAGGCGAATTTGAAAAAATTCGTCATCAAATTCATGAAAATCCTGAGCT
TGGTTTTGATGAATTATGTACTGCAAAATTAGTGGCGCAAAAATTAAAAGAATTTGGTTATGAGGTTTATGAGGAAATAG
GAAAAACAGGCGTTGTGGGGGTTTTAAAAAAGGGGAATAGCGATAAAAAAATAGGACTTCGTGCAGATATGGATGCTTTG
CCTTTGCAAGAATGCACAAATTTGCCTTATAAAAGCAAAAAAGAAAATGTAATGCATGCTTGCGGTCATGATGGACATAC
TACTTCTTTATTGCTTGCTGCAAAGTATTTAGCAAGTCAGAATTTTAATGGCACTTTAAATCTTTATTTTCAACCTGCTG
AAGAGGGTTTGGGTGGTGCTAAGGCAATGATAGAAGATGGATTGTTTGAAAAATTTGATAGTGCTTATGTTTTTGGATGG
CACAATATGCCTTTTGGTAGCGATAAGAAATTTTATCTTAAAAAAGGTGCGATGATGGCTTCTTCGGATAGTTATAGCAT
TGAAGTTATTGGAAGAGGTGGTCATGGAAGTGCTCCAGAAAAGGCAAAAGATCCTATTTATGCTGCTTCTTTACTTGTTG
TGGCTTTACAAAGCATAGTATCTCGCAATGTTGATCCCCAAAATTCAGCAGTTGTAAGCATAGGAGCTTTTAATGCAGGA
CATGCTTTTAATATCATTCCAGATATTGCAACGATTAAAATGAGTGTTAGAGCATTAGATAATGAAACTAGAAAGCTAAC
TGAAGAAAAAATTTATAAAATTTGTAAAGGTCTTGCACAGGCTAATGATATAGAGATTAAAATCAATAAAAATGTTGTTG
CACCAGTGACTATGAATAACGATGAAGCTGTGGATTTTGCTAGTGAGGTTGCAAAAGAATTATTTGGCGAAAAAAATTGT
GAATTTAATCATCGTCCTTTAATGGCAAGTGAGGATTTTGGATTTTTTTGCGAAATGAAAAAATGTGCCTATGCTTTTTT
AGAAAATGAAAACGACATTTATTTACATAATTCTAGTTATGTTTTTAATGATAAACTTTTAGCTAGGGCTGCAAGTTATT
ATGCGAAGCTAGCTTTAAAATACTTAAAATAA

Upstream 100 bases:

>100_bases
TTGGGTTTTGTTTATATTTTTGCTTTGATTTTTATTTCAGCGATCTTAGCACAATTTGTTTTACCTAGAAGAGAAAATTT
TATACAAGGAGAAAAATAGA

Downstream 100 bases:

>100_bases
AAACTAATCTAGAATTTCAAGCGCAATTTCTTCCTTTAAACCTTTGATTTGAATTTCTTTGAGTTTTAGATTTGAAGGAA
GGTTTGCTAAAAGCTCTTTA

Product: hippurate hydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MNLIPEILDLQGEFEKIRHQIHENPELGFDELCTAKLVAQKLKEFGYEVYEEIGKTGVVGVLKKGNSDKKIGLRADMDAL
PLQECTNLPYKSKKENVMHACGHDGHTTSLLLAAKYLASQNFNGTLNLYFQPAEEGLGGAKAMIEDGLFEKFDSAYVFGW
HNMPFGSDKKFYLKKGAMMASSDSYSIEVIGRGGHGSAPEKAKDPIYAASLLVVALQSIVSRNVDPQNSAVVSIGAFNAG
HAFNIIPDIATIKMSVRALDNETRKLTEEKIYKICKGLAQANDIEIKINKNVVAPVTMNNDEAVDFASEVAKELFGEKNC
EFNHRPLMASEDFGFFCEMKKCAYAFLENENDIYLHNSSYVFNDKLLARAASYYAKLALKYLK

Sequences:

>Translated_383_residues
MNLIPEILDLQGEFEKIRHQIHENPELGFDELCTAKLVAQKLKEFGYEVYEEIGKTGVVGVLKKGNSDKKIGLRADMDAL
PLQECTNLPYKSKKENVMHACGHDGHTTSLLLAAKYLASQNFNGTLNLYFQPAEEGLGGAKAMIEDGLFEKFDSAYVFGW
HNMPFGSDKKFYLKKGAMMASSDSYSIEVIGRGGHGSAPEKAKDPIYAASLLVVALQSIVSRNVDPQNSAVVSIGAFNAG
HAFNIIPDIATIKMSVRALDNETRKLTEEKIYKICKGLAQANDIEIKINKNVVAPVTMNNDEAVDFASEVAKELFGEKNC
EFNHRPLMASEDFGFFCEMKKCAYAFLENENDIYLHNSSYVFNDKLLARAASYYAKLALKYLK
>Mature_383_residues
MNLIPEILDLQGEFEKIRHQIHENPELGFDELCTAKLVAQKLKEFGYEVYEEIGKTGVVGVLKKGNSDKKIGLRADMDAL
PLQECTNLPYKSKKENVMHACGHDGHTTSLLLAAKYLASQNFNGTLNLYFQPAEEGLGGAKAMIEDGLFEKFDSAYVFGW
HNMPFGSDKKFYLKKGAMMASSDSYSIEVIGRGGHGSAPEKAKDPIYAASLLVVALQSIVSRNVDPQNSAVVSIGAFNAG
HAFNIIPDIATIKMSVRALDNETRKLTEEKIYKICKGLAQANDIEIKINKNVVAPVTMNNDEAVDFASEVAKELFGEKNC
EFNHRPLMASEDFGFFCEMKKCAYAFLENENDIYLHNSSYVFNDKLLARAASYYAKLALKYLK

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=396, Percent_Identity=25, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 42519; Mature: 42519

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLIPEILDLQGEFEKIRHQIHENPELGFDELCTAKLVAQKLKEFGYEVYEEIGKTGVVG
CCCCCHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VLKKGNSDKKIGLRADMDALPLQECTNLPYKSKKENVMHACGHDGHTTSLLLAAKYLASQ
EEECCCCCCEEEEECCCCCCCHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCC
NFNGTLNLYFQPAEEGLGGAKAMIEDGLFEKFDSAYVFGWHNMPFGSDKKFYLKKGAMMA
CCCCEEEEEEECHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCEEE
SSDSYSIEVIGRGGHGSAPEKAKDPIYAASLLVVALQSIVSRNVDPQNSAVVSIGAFNAG
CCCCEEEEEEECCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
HAFNIIPDIATIKMSVRALDNETRKLTEEKIYKICKGLAQANDIEIKINKNVVAPVTMNN
CEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEECC
DEAVDFASEVAKELFGEKNCEFNHRPLMASEDFGFFCEMKKCAYAFLENENDIYLHNSSY
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCE
VFNDKLLARAASYYAKLALKYLK
EECHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLIPEILDLQGEFEKIRHQIHENPELGFDELCTAKLVAQKLKEFGYEVYEEIGKTGVVG
CCCCCHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VLKKGNSDKKIGLRADMDALPLQECTNLPYKSKKENVMHACGHDGHTTSLLLAAKYLASQ
EEECCCCCCEEEEECCCCCCCHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCC
NFNGTLNLYFQPAEEGLGGAKAMIEDGLFEKFDSAYVFGWHNMPFGSDKKFYLKKGAMMA
CCCCEEEEEEECHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCEEE
SSDSYSIEVIGRGGHGSAPEKAKDPIYAASLLVVALQSIVSRNVDPQNSAVVSIGAFNAG
CCCCEEEEEEECCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
HAFNIIPDIATIKMSVRALDNETRKLTEEKIYKICKGLAQANDIEIKINKNVVAPVTMNN
CEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEECC
DEAVDFASEVAKELFGEKNCEFNHRPLMASEDFGFFCEMKKCAYAFLENENDIYLHNSSY
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCE
VFNDKLLARAASYYAKLALKYLK
EECHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]