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The map label for this gene is cmoB [H]

Identifier: 57237803

GI number: 57237803

Start: 988820

End: 989695

Strand: Direct

Name: cmoB [H]

Synonym: CJE1058

Alternate gene names: 57237803

Gene position: 988820-989695 (Clockwise)

Preceding gene: 57237801

Following gene: 57237804

Centisome position: 55.62

GC content: 30.02

Gene sequence:

>876_bases
ATGCAAGAAAATTTACTTGAAAAACAATTTCTAAATCACCCTTTATACGCTAAAATTCAAGAACTTAAAGCTCTCAATCT
TGCTTGTAATTTTTCTTTAGATGATAGTGTAAATTTATCTACAAATTCTCAAGCCAAAGATGAAATCTTAGCTATAGCAA
AAGAACTTAAACCATGGAGAAAAGGTCCTTTTAAAATAGACGATCTTTTCATCGATACAGAATGGCAAAGTTTTATTAAA
TTCAACATTCTAAAGCCTTTCATGAACGAAATTTCTCAAAAATGCGTTGCTGATATAGGCTGTAATAATGGTTATTATAT
GTTTAAAATGCTTGAATTTAATCCTGCAAAACTCATAGGTTTTGATCCTTCTATCAAATACCGTTTGCAATTTGAACTCA
TTAACGCTTTAGCTAAAACTCCTATAAAATACGAACTTTTAGGCGTAGAAGATCTTCCAAGCTATGGCTTGAAATTTGAT
GTGATTTTCTGTCTTGGGGTGATTTATCATAGAAGTGATCCTATAAAAATGCTAAAAGATTTAAAAGCAGGACTTAATAA
AAATGGAGTTGTTTTTTTAGATACCATGTATATAGAAGATGAGAGAGAAATCGCTCTTGTGCCTAATAAAACTTATTCTA
AAATTCCAAATATTTATTTTGTACCAAGCATTAGTGCATTAAAAAACTGGTGTGAAAGAGCAGGATTTAAAGAATTTGAA
GTTTTGGCTACAAAAAAAACCGATGAAAACGAACAAAGAAAAACAGAATGGATAGACTCTTTTTCGCTGGAAAATTTCTT
AGATCCAAAGGATAAAAATCTTACCATTGAAGGCTATGAAGCACCCAAAAGAGTTTATATACGCATTAAAATATAA

Upstream 100 bases:

>100_bases
TGAGTTATTTTTGATCAAATATTTAATGCATATAATTTATATTGCCATAAAAGCTTTTAATGTTATAATTTAGCATTAAA
AGCAAATGTCAAGGATAAAA

Downstream 100 bases:

>100_bases
ATTCTTTTTATTTTTTACCGATATTGAAAATTAGATATTTTGAAGTAGATAAGTAGAATTTAAAGGTTAGATATGGCAAA
AAAAGATAATTTTGAAGAAT

Product: methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MQENLLEKQFLNHPLYAKIQELKALNLACNFSLDDSVNLSTNSQAKDEILAIAKELKPWRKGPFKIDDLFIDTEWQSFIK
FNILKPFMNEISQKCVADIGCNNGYYMFKMLEFNPAKLIGFDPSIKYRLQFELINALAKTPIKYELLGVEDLPSYGLKFD
VIFCLGVIYHRSDPIKMLKDLKAGLNKNGVVFLDTMYIEDEREIALVPNKTYSKIPNIYFVPSISALKNWCERAGFKEFE
VLATKKTDENEQRKTEWIDSFSLENFLDPKDKNLTIEGYEAPKRVYIRIKI

Sequences:

>Translated_291_residues
MQENLLEKQFLNHPLYAKIQELKALNLACNFSLDDSVNLSTNSQAKDEILAIAKELKPWRKGPFKIDDLFIDTEWQSFIK
FNILKPFMNEISQKCVADIGCNNGYYMFKMLEFNPAKLIGFDPSIKYRLQFELINALAKTPIKYELLGVEDLPSYGLKFD
VIFCLGVIYHRSDPIKMLKDLKAGLNKNGVVFLDTMYIEDEREIALVPNKTYSKIPNIYFVPSISALKNWCERAGFKEFE
VLATKKTDENEQRKTEWIDSFSLENFLDPKDKNLTIEGYEAPKRVYIRIKI
>Mature_291_residues
MQENLLEKQFLNHPLYAKIQELKALNLACNFSLDDSVNLSTNSQAKDEILAIAKELKPWRKGPFKIDDLFIDTEWQSFIK
FNILKPFMNEISQKCVADIGCNNGYYMFKMLEFNPAKLIGFDPSIKYRLQFELINALAKTPIKYELLGVEDLPSYGLKFD
VIFCLGVIYHRSDPIKMLKDLKAGLNKNGVVFLDTMYIEDEREIALVPNKTYSKIPNIYFVPSISALKNWCERAGFKEFE
VLATKKTDENEQRKTEWIDSFSLENFLDPKDKNLTIEGYEAPKRVYIRIKI

Specific function: Catalyzes the conversion of 5-hydroxyuridine (ho5U) to 5-methoxyuridine (mo5U) at position 34 in tRNA [H]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. CmoB family [H]

Homologues:

Organism=Escherichia coli, GI1788178, Length=242, Percent_Identity=38.0165289256198, Blast_Score=197, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010017 [H]

Pfam domain/function: PF08003 Methyltransf_9 [H]

EC number: NA

Molecular weight: Translated: 33815; Mature: 33815

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQENLLEKQFLNHPLYAKIQELKALNLACNFSLDDSVNLSTNSQAKDEILAIAKELKPWR
CCCHHHHHHHHCCCHHHHHHHHHEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCHHH
KGPFKIDDLFIDTEWQSFIKFNILKPFMNEISQKCVADIGCNNGYYMFKMLEFNPAKLIG
CCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEE
FDPSIKYRLQFELINALAKTPIKYELLGVEDLPSYGLKFDVIFCLGVIYHRSDPIKMLKD
CCCCEEEEEHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
LKAGLNKNGVVFLDTMYIEDEREIALVPNKTYSKIPNIYFVPSISALKNWCERAGFKEFE
HHHCCCCCCEEEEEEEEEECCCCEEEECCCHHHHCCCEEEECCHHHHHHHHHHCCCCEEE
VLATKKTDENEQRKTEWIDSFSLENFLDPKDKNLTIEGYEAPKRVYIRIKI
EEEECCCCCHHHHHHHHHHHHCHHHCCCCCCCCEEEECCCCCEEEEEEEEC
>Mature Secondary Structure
MQENLLEKQFLNHPLYAKIQELKALNLACNFSLDDSVNLSTNSQAKDEILAIAKELKPWR
CCCHHHHHHHHCCCHHHHHHHHHEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCHHH
KGPFKIDDLFIDTEWQSFIKFNILKPFMNEISQKCVADIGCNNGYYMFKMLEFNPAKLIG
CCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEE
FDPSIKYRLQFELINALAKTPIKYELLGVEDLPSYGLKFDVIFCLGVIYHRSDPIKMLKD
CCCCEEEEEHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
LKAGLNKNGVVFLDTMYIEDEREIALVPNKTYSKIPNIYFVPSISALKNWCERAGFKEFE
HHHCCCCCCEEEEEEEEEECCCCEEEECCCHHHHCCCEEEECCHHHHHHHHHHCCCCEEE
VLATKKTDENEQRKTEWIDSFSLENFLDPKDKNLTIEGYEAPKRVYIRIKI
EEEECCCCCHHHHHHHHHHHHCHHHCCCCCCCCEEEECCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA