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The map label for this gene is purH [H]

Identifier: 57237781

GI number: 57237781

Start: 970677

End: 972209

Strand: Reverse

Name: purH [H]

Synonym: CJE1033

Alternate gene names: 57237781

Gene position: 972209-970677 (Counterclockwise)

Preceding gene: 57237782

Following gene: 57237780

Centisome position: 54.69

GC content: 33.86

Gene sequence:

>1533_bases
ATGAGAGCATTGCTTAGTGTGAGTGATAAAGAAGGTATAGTAGAATTTGGCAAAGAGCTTGAAAATTTAGGCTTTGAGAT
TCTTTCAACAGGTGGGACTTTTAAGTTTTTAAAAGAAAATGGAATAAAAGTTATGGAAGTGAGTGATTTTACAAAAAGTC
CTGAGCTTTTTGAAGGGCGTGTAAAGACTTTACACCCTAAGATTCATGGTGGAATTTTACACAAAAGAAGCAATGAAAAT
CATATCAAACAAGCAAAAGAAAATGAAATTTTAGGAATTGATCTAGTTTGTGTGAATTTATATCCTTTTAAAAAAACCAC
TATCATGAGTGATGATTTTGATGAAATTATAGAAAATATCGATATAGGTGGGCCTGCGATGATAAGAAGTGCAGCAAAGA
ACTATAAAGATGTGATGGTACTTTGCGATCCGCTTGATTATGAAAAAGTCATCGAGACTTTAAAAAAAGGTCAAATTGAT
GAGAATTTTCGTTTAAATTTGATGATAAAAGCTTATGAGCATACGGCAAATTATGATGCTTATATTGCAAATTATATGAA
TGAACGCTTTAACGGAGGTTTTGGAGCGAGTAAATTTATAGTAGGACAAAAAGTATTTGATACAAAATATGGAGAAAATC
CACATCAAAAAGGGGCTTTATACGAGTTTGATGCCTTTTTTAGTGCAAATTTCAAAGCCCTTAAAGGCGAAGCAAGTTTT
AATAATCTAACCGATATTAACGCAGCTTTAAATTTGGCTAGTAGTTTCGATAAAGCTCCAGCTATTGCTATAGTAAAACA
TGGAAATCCTTGCGGTTTTGCTATAAAAGAAAATTTAGTACAAAGCTATATACATGCTTTAAAATGCGATAGCGTTAGTG
CTTATGGAGGAGTGGTTGCAATCAATGGAACTCTTGATGAGGCTTTAGCAAATAAAATCAATGAAATTTATGTAGAAGTA
ATCATCGCAGCCAATGTAGATGAAAAAGCTTTAGCTGTGTTTGAAGGTAAAAAACGCATTAAAATTTTCACTCAAGAAAG
CTCGTTTTTAATCCGCAGTTTTGATAAATATGATTTTAAACATATTGATGGGGGTTTTGTATATCAAAACAGCGATGAAG
TTGGCGAAGATGAGTTTAAAAATGCAAAGCTTATGAGTCAAAGAGAGGCAAGTAAGGAAGAATTAAAAGATCTTGAAATC
GCCATGAAAATAGCAGCATTTACCAAATCAAACAATGTTGTTTATGTAAAAAATGGCGCTATGGTGGCTATAGGCATGGG
TATGACAAGCAGAATCGATGCAGCAAAGGTAGCAATCGCTAAAGCTAAAGAAATGGGGCTTGATTTGCAAGGTTGCGTTT
TAGCAAGTGAAGCCTTTTTTCCTTTTAGAGATAGCATAGATGAAGCAAGTAAAGTAGGAGTTAAAGCTATAGTAGAACCA
GGCGGAAGTATAAGAGATGATGAAGTGGTAAAGGCGGCTGATGAGTATGGTATGGCGCTTTATTTTACAGGAGTAAGACA
TTTCTTACATTAA

Upstream 100 bases:

>100_bases
CAAACAATGTTAGTGAAGAAGAGCTTAAAATCGGTGTAGGGAAATTTCAAAAAATCAACGAAGCTTACGAAAAAGTTAAA
AAACATTTAGAAAGGTAAAA

Downstream 100 bases:

>100_bases
GTTTAATGGAATGTTATGTTTTTTACACATAATTCAATAAAAATTAAATAAATTATGTGTAAAATATTTCCGAAAAATTT
TATTTTTAGGAAACTATGAT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MRALLSVSDKEGIVEFGKELENLGFEILSTGGTFKFLKENGIKVMEVSDFTKSPELFEGRVKTLHPKIHGGILHKRSNEN
HIKQAKENEILGIDLVCVNLYPFKKTTIMSDDFDEIIENIDIGGPAMIRSAAKNYKDVMVLCDPLDYEKVIETLKKGQID
ENFRLNLMIKAYEHTANYDAYIANYMNERFNGGFGASKFIVGQKVFDTKYGENPHQKGALYEFDAFFSANFKALKGEASF
NNLTDINAALNLASSFDKAPAIAIVKHGNPCGFAIKENLVQSYIHALKCDSVSAYGGVVAINGTLDEALANKINEIYVEV
IIAANVDEKALAVFEGKKRIKIFTQESSFLIRSFDKYDFKHIDGGFVYQNSDEVGEDEFKNAKLMSQREASKEELKDLEI
AMKIAAFTKSNNVVYVKNGAMVAIGMGMTSRIDAAKVAIAKAKEMGLDLQGCVLASEAFFPFRDSIDEASKVGVKAIVEP
GGSIRDDEVVKAADEYGMALYFTGVRHFLH

Sequences:

>Translated_510_residues
MRALLSVSDKEGIVEFGKELENLGFEILSTGGTFKFLKENGIKVMEVSDFTKSPELFEGRVKTLHPKIHGGILHKRSNEN
HIKQAKENEILGIDLVCVNLYPFKKTTIMSDDFDEIIENIDIGGPAMIRSAAKNYKDVMVLCDPLDYEKVIETLKKGQID
ENFRLNLMIKAYEHTANYDAYIANYMNERFNGGFGASKFIVGQKVFDTKYGENPHQKGALYEFDAFFSANFKALKGEASF
NNLTDINAALNLASSFDKAPAIAIVKHGNPCGFAIKENLVQSYIHALKCDSVSAYGGVVAINGTLDEALANKINEIYVEV
IIAANVDEKALAVFEGKKRIKIFTQESSFLIRSFDKYDFKHIDGGFVYQNSDEVGEDEFKNAKLMSQREASKEELKDLEI
AMKIAAFTKSNNVVYVKNGAMVAIGMGMTSRIDAAKVAIAKAKEMGLDLQGCVLASEAFFPFRDSIDEASKVGVKAIVEP
GGSIRDDEVVKAADEYGMALYFTGVRHFLH
>Mature_510_residues
MRALLSVSDKEGIVEFGKELENLGFEILSTGGTFKFLKENGIKVMEVSDFTKSPELFEGRVKTLHPKIHGGILHKRSNEN
HIKQAKENEILGIDLVCVNLYPFKKTTIMSDDFDEIIENIDIGGPAMIRSAAKNYKDVMVLCDPLDYEKVIETLKKGQID
ENFRLNLMIKAYEHTANYDAYIANYMNERFNGGFGASKFIVGQKVFDTKYGENPHQKGALYEFDAFFSANFKALKGEASF
NNLTDINAALNLASSFDKAPAIAIVKHGNPCGFAIKENLVQSYIHALKCDSVSAYGGVVAINGTLDEALANKINEIYVEV
IIAANVDEKALAVFEGKKRIKIFTQESSFLIRSFDKYDFKHIDGGFVYQNSDEVGEDEFKNAKLMSQREASKEELKDLEI
AMKIAAFTKSNNVVYVKNGAMVAIGMGMTSRIDAAKVAIAKAKEMGLDLQGCVLASEAFFPFRDSIDEASKVGVKAIVEP
GGSIRDDEVVKAADEYGMALYFTGVRHFLH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=485, Percent_Identity=32.5773195876289, Blast_Score=220, Evalue=3e-57,
Organism=Escherichia coli, GI1790439, Length=529, Percent_Identity=44.4234404536862, Blast_Score=430, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71985564, Length=499, Percent_Identity=32.064128256513, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI71985574, Length=331, Percent_Identity=25.6797583081571, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71985556, Length=60, Percent_Identity=50, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323768, Length=488, Percent_Identity=31.9672131147541, Blast_Score=226, Evalue=8e-60,
Organism=Saccharomyces cerevisiae, GI6323056, Length=488, Percent_Identity=32.172131147541, Blast_Score=219, Evalue=7e-58,
Organism=Drosophila melanogaster, GI24649832, Length=473, Percent_Identity=33.1923890063425, Blast_Score=229, Evalue=4e-60,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56505; Mature: 56505

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALLSVSDKEGIVEFGKELENLGFEILSTGGTFKFLKENGIKVMEVSDFTKSPELFEGR
CCCEECCCCCCHHHHHHHHHHHCCEEEEECCCCEEEEECCCCEEEEECCCCCCCHHHCCC
VKTLHPKIHGGILHKRSNENHIKQAKENEILGIDLVCVNLYPFKKTTIMSDDFDEIIENI
HHHCCCHHHCCEEECCCCCHHHHHCCCCCEEEEEEEEEEEECCCCEEECCCCHHHHHHHC
DIGGPAMIRSAAKNYKDVMVLCDPLDYEKVIETLKKGQIDENFRLNLMIKAYEHTANYDA
CCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCHH
YIANYMNERFNGGFGASKFIVGQKVFDTKYGENPHQKGALYEFDAFFSANFKALKGEASF
HHHHHHHHHCCCCCCCCEEEECCHHHCCCCCCCCCCCCCEEEEEHHHCCCCEEEECCCCC
NNLTDINAALNLASSFDKAPAIAIVKHGNPCGFAIKENLVQSYIHALKCDSVSAYGGVVA
CCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEE
INGTLDEALANKINEIYVEVIIAANVDEKALAVFEGKKRIKIFTQESSFLIRSFDKYDFK
ECCCHHHHHHHHHHHEEEEEEEEECCCHHHHHEECCCCEEEEEEECCCHHHHHCCCCCCE
HIDGGFVYQNSDEVGEDEFKNAKLMSQREASKEELKDLEIAMKIAAFTKSNNVVYVKNGA
ECCCCEEECCCCCCCCHHHHCHHHHHHHHCCHHHHHHHHHHHHEEEEECCCCEEEEECCC
MVAIGMGMTSRIDAAKVAIAKAKEMGLDLQGCVLASEAFFPFRDSIDEASKVGVKAIVEP
EEEEECCCCCCCCHHHHHHHHHHHHCCCCHHEEEECCCCCCCHHCHHHHHHCCEEEEECC
GGSIRDDEVVKAADEYGMALYFTGVRHFLH
CCCCCCHHHHHHHHHCCEEEEEEHHHHHCC
>Mature Secondary Structure
MRALLSVSDKEGIVEFGKELENLGFEILSTGGTFKFLKENGIKVMEVSDFTKSPELFEGR
CCCEECCCCCCHHHHHHHHHHHCCEEEEECCCCEEEEECCCCEEEEECCCCCCCHHHCCC
VKTLHPKIHGGILHKRSNENHIKQAKENEILGIDLVCVNLYPFKKTTIMSDDFDEIIENI
HHHCCCHHHCCEEECCCCCHHHHHCCCCCEEEEEEEEEEEECCCCEEECCCCHHHHHHHC
DIGGPAMIRSAAKNYKDVMVLCDPLDYEKVIETLKKGQIDENFRLNLMIKAYEHTANYDA
CCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCHH
YIANYMNERFNGGFGASKFIVGQKVFDTKYGENPHQKGALYEFDAFFSANFKALKGEASF
HHHHHHHHHCCCCCCCCEEEECCHHHCCCCCCCCCCCCCEEEEEHHHCCCCEEEECCCCC
NNLTDINAALNLASSFDKAPAIAIVKHGNPCGFAIKENLVQSYIHALKCDSVSAYGGVVA
CCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEE
INGTLDEALANKINEIYVEVIIAANVDEKALAVFEGKKRIKIFTQESSFLIRSFDKYDFK
ECCCHHHHHHHHHHHEEEEEEEEECCCHHHHHEECCCCEEEEEEECCCHHHHHCCCCCCE
HIDGGFVYQNSDEVGEDEFKNAKLMSQREASKEELKDLEIAMKIAAFTKSNNVVYVKNGA
ECCCCEEECCCCCCCCHHHHCHHHHHHHHCCHHHHHHHHHHHHEEEEECCCCEEEEECCC
MVAIGMGMTSRIDAAKVAIAKAKEMGLDLQGCVLASEAFFPFRDSIDEASKVGVKAIVEP
EEEEECCCCCCCCHHHHHHHHHHHHCCCCHHEEEECCCCCCCHHCHHHHHHCCEEEEECC
GGSIRDDEVVKAADEYGMALYFTGVRHFLH
CCCCCCHHHHHHHHHCCEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA