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The map label for this gene is pckA [H]

Identifier: 57237760

GI number: 57237760

Start: 944364

End: 945938

Strand: Reverse

Name: pckA [H]

Synonym: CJE1010

Alternate gene names: 57237760

Gene position: 945938-944364 (Counterclockwise)

Preceding gene: 57237761

Following gene: 57237759

Centisome position: 53.21

GC content: 32.83

Gene sequence:

>1575_bases
ATGAAAAAATTTGATAAGTTAGGTTTGGATAATATAAAGGAAATTTTTCATAATTTAAGTTATGATGAATTAAATGCTCA
TGAAAAAGCAAATAATGAAGGTTTAAGCACGGATAATGATACTTTTTGTGTAGATACAGGGATTTTTACAGGTAGAAGTC
CTAAAGATAAATATTTTGTAAAACAAGATCCATCTAGTAAATATATAGCTTGGGGAAAGGTAAATCAGCCTATTACAAAA
GAATTATTTGATAAACTTTTAACTAAAGCAAAGCAAGAATTAAGCGGAAAGAAAATTTATGTTCAAGATGTTTTTTGCGG
TGCTTCTTTGCAAAGTCGCAAAGCAGTAAGATTTGTTACTGAAATTGCATGGCAAGCACATTTTGTTAAAAATATGTTTA
TTCGCCCAAGTCAAGAGGAACTTGAGAATTTTAAAGCAGATTTTATTGTTTATAATGCTTGCAAATGCATCAATGAAGAT
TACAAACAAGATGGTTTAAATTCAGAGGTTTTTGTGATCTTTAATGTTGAAGAAAATATAGCGGTTATTGGTGGAACTTG
GTATGGTGGAGAAATGAAAAAAGGTATTTTTTCTATGATGAATTATTGGTTGCCACTAGAAAATAAACTTTCTATGCATT
GTAGTGCAAATGTCGGAGAAAAAGACGATGTGGCACTTTTCTTTGGACTTAGCGGTACAGGAAAAACTACGCTTTCAACC
GATCCAAAAAGAAGACTCATAGGTGATGATGAGCATGGCTGGGATGATGAAGGTGTGTTTAATTTTGAGGGCGGTTGCTA
TGCAAAAACAATCAATCTTAATCCTGAACATGAGCCTGAAATTTATGGAGCCATTAAAAGAAATGCACTTTTAGAAAATG
TAGTTTTAAGAGCTGATAAGAGTGTTGATTATGCTGATGCCTCAAAAACAGAAAACACGAGAGTTTCTTATCCTATAGAG
CATATTGAAAATCATGAGCCTAGTCTAAAAGCAGGGCATCCAAAAAATATTATATTTTTAAGTGCAGATGCTTTTGGAAT
TTTACCCCCGGTGAGCAAACTAAGCAAAGAGCAGGCTATGTATTATTTTTTAAGTGGCTATACAGCAAAAGTAGCTGGAA
CTGAGCGCGGTATAACAGAGCCTCAAGCAACTTTTTCAGCTTGTTTTGGAGAACCTTTTATGCCTTTACATCCAACGGTT
TATGCGAGATTGCTTGGTGAAAAGATTGAAAAACATGAAGTTAATGTTTATCTTGTTAATACGGGTTGGAGTGGCGGAAG
CTACAGTGTGGGTAAAAGAATGAGCATTAAGGCTACTAGGGCTTGCATTAATGCTATTCTAGATGGAAGTATTACAAAAT
GCGAATTTGAAAATTTTGAAGTGTTTGATTTAGCTATTCCAAAAGCTTTAGAAGGTGTAGAAAGTGTACTTTTAAATCCT
ATAAATACTTGGTTAGATAAAAATGCTTATATTGCAACAAGAGATAAATTAGCACATATGTTTATACAAAATTTTAAACG
TTATGAAGATGTCAAAGAAGGCATTGAATTTAGCAAATTTGGACCAAAAATTTAA

Upstream 100 bases:

>100_bases
CCCAAAAGATGGAATTATTTCAGAGCTTTGTATTAAAATAGGTGATACTGTTAATGAAGGCGAAGTATTAGCTATTTACA
AGAATTAAAGTGAGGGAAAA

Downstream 100 bases:

>100_bases
GGCATTTAAATGAAAAATGAAATGTGGTCAGGACGTTTTAGTGGTGCAAGTGATGAGCTTTTAAAAGAATTTAATGCAAG
TTTAAATGTAGATAAAACTT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MKKFDKLGLDNIKEIFHNLSYDELNAHEKANNEGLSTDNDTFCVDTGIFTGRSPKDKYFVKQDPSSKYIAWGKVNQPITK
ELFDKLLTKAKQELSGKKIYVQDVFCGASLQSRKAVRFVTEIAWQAHFVKNMFIRPSQEELENFKADFIVYNACKCINED
YKQDGLNSEVFVIFNVEENIAVIGGTWYGGEMKKGIFSMMNYWLPLENKLSMHCSANVGEKDDVALFFGLSGTGKTTLST
DPKRRLIGDDEHGWDDEGVFNFEGGCYAKTINLNPEHEPEIYGAIKRNALLENVVLRADKSVDYADASKTENTRVSYPIE
HIENHEPSLKAGHPKNIIFLSADAFGILPPVSKLSKEQAMYYFLSGYTAKVAGTERGITEPQATFSACFGEPFMPLHPTV
YARLLGEKIEKHEVNVYLVNTGWSGGSYSVGKRMSIKATRACINAILDGSITKCEFENFEVFDLAIPKALEGVESVLLNP
INTWLDKNAYIATRDKLAHMFIQNFKRYEDVKEGIEFSKFGPKI

Sequences:

>Translated_524_residues
MKKFDKLGLDNIKEIFHNLSYDELNAHEKANNEGLSTDNDTFCVDTGIFTGRSPKDKYFVKQDPSSKYIAWGKVNQPITK
ELFDKLLTKAKQELSGKKIYVQDVFCGASLQSRKAVRFVTEIAWQAHFVKNMFIRPSQEELENFKADFIVYNACKCINED
YKQDGLNSEVFVIFNVEENIAVIGGTWYGGEMKKGIFSMMNYWLPLENKLSMHCSANVGEKDDVALFFGLSGTGKTTLST
DPKRRLIGDDEHGWDDEGVFNFEGGCYAKTINLNPEHEPEIYGAIKRNALLENVVLRADKSVDYADASKTENTRVSYPIE
HIENHEPSLKAGHPKNIIFLSADAFGILPPVSKLSKEQAMYYFLSGYTAKVAGTERGITEPQATFSACFGEPFMPLHPTV
YARLLGEKIEKHEVNVYLVNTGWSGGSYSVGKRMSIKATRACINAILDGSITKCEFENFEVFDLAIPKALEGVESVLLNP
INTWLDKNAYIATRDKLAHMFIQNFKRYEDVKEGIEFSKFGPKI
>Mature_524_residues
MKKFDKLGLDNIKEIFHNLSYDELNAHEKANNEGLSTDNDTFCVDTGIFTGRSPKDKYFVKQDPSSKYIAWGKVNQPITK
ELFDKLLTKAKQELSGKKIYVQDVFCGASLQSRKAVRFVTEIAWQAHFVKNMFIRPSQEELENFKADFIVYNACKCINED
YKQDGLNSEVFVIFNVEENIAVIGGTWYGGEMKKGIFSMMNYWLPLENKLSMHCSANVGEKDDVALFFGLSGTGKTTLST
DPKRRLIGDDEHGWDDEGVFNFEGGCYAKTINLNPEHEPEIYGAIKRNALLENVVLRADKSVDYADASKTENTRVSYPIE
HIENHEPSLKAGHPKNIIFLSADAFGILPPVSKLSKEQAMYYFLSGYTAKVAGTERGITEPQATFSACFGEPFMPLHPTV
YARLLGEKIEKHEVNVYLVNTGWSGGSYSVGKRMSIKATRACINAILDGSITKCEFENFEVFDLAIPKALEGVESVLLNP
INTWLDKNAYIATRDKLAHMFIQNFKRYEDVKEGIEFSKFGPKI

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=535, Percent_Identity=56.6355140186916, Blast_Score=620, Evalue=1e-179,
Organism=Saccharomyces cerevisiae, GI6322950, Length=487, Percent_Identity=48.4599589322382, Blast_Score=450, Evalue=1e-127,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 59084; Mature: 59084

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFDKLGLDNIKEIFHNLSYDELNAHEKANNEGLSTDNDTFCVDTGIFTGRSPKDKYFV
CCCHHHCCHHHHHHHHHCCCHHHHCCHHCCCCCCCCCCCCCEEEECCEECCCCCCCCEEE
KQDPSSKYIAWGKVNQPITKELFDKLLTKAKQELSGKKIYVQDVFCGASLQSRKAVRFVT
EECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHH
EIAWQAHFVKNMFIRPSQEELENFKADFIVYNACKCINEDYKQDGLNSEVFVIFNVEENI
HHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEHHHHHHCHHHHHCCCCCCEEEEEEECCCE
AVIGGTWYGGEMKKGIFSMMNYWLPLENKLSMHCSANVGEKDDVALFFGLSGTGKTTLST
EEEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCC
DPKRRLIGDDEHGWDDEGVFNFEGGCYAKTINLNPEHEPEIYGAIKRNALLENVVLRADK
CCHHHCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCC
SVDYADASKTENTRVSYPIEHIENHEPSLKAGHPKNIIFLSADAFGILPPVSKLSKEQAM
CCCCCCCCCCCCCEEECCHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHEE
YYFLSGYTAKVAGTERGITEPQATFSACFGEPFMPLHPTVYARLLGEKIEKHEVNVYLVN
EEEECCCEEEECCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCEEEEEEEE
TGWSGGSYSVGKRMSIKATRACINAILDGSITKCEFENFEVFDLAIPKALEGVESVLLNP
CCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCEEEECCCEEEEEEHHHHHHHHHHHHHHHH
INTWLDKNAYIATRDKLAHMFIQNFKRYEDVKEGIEFSKFGPKI
HHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKKFDKLGLDNIKEIFHNLSYDELNAHEKANNEGLSTDNDTFCVDTGIFTGRSPKDKYFV
CCCHHHCCHHHHHHHHHCCCHHHHCCHHCCCCCCCCCCCCCEEEECCEECCCCCCCCEEE
KQDPSSKYIAWGKVNQPITKELFDKLLTKAKQELSGKKIYVQDVFCGASLQSRKAVRFVT
EECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHH
EIAWQAHFVKNMFIRPSQEELENFKADFIVYNACKCINEDYKQDGLNSEVFVIFNVEENI
HHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEHHHHHHCHHHHHCCCCCCEEEEEEECCCE
AVIGGTWYGGEMKKGIFSMMNYWLPLENKLSMHCSANVGEKDDVALFFGLSGTGKTTLST
EEEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCC
DPKRRLIGDDEHGWDDEGVFNFEGGCYAKTINLNPEHEPEIYGAIKRNALLENVVLRADK
CCHHHCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCC
SVDYADASKTENTRVSYPIEHIENHEPSLKAGHPKNIIFLSADAFGILPPVSKLSKEQAM
CCCCCCCCCCCCCEEECCHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHEE
YYFLSGYTAKVAGTERGITEPQATFSACFGEPFMPLHPTVYARLLGEKIEKHEVNVYLVN
EEEECCCEEEECCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCEEEEEEEE
TGWSGGSYSVGKRMSIKATRACINAILDGSITKCEFENFEVFDLAIPKALEGVESVLLNP
CCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCEEEECCCEEEEEEHHHHHHHHHHHHHHHH
INTWLDKNAYIATRDKLAHMFIQNFKRYEDVKEGIEFSKFGPKI
HHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA