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The map label for this gene is ogt [H]

Identifier: 57237677

GI number: 57237677

Start: 862589

End: 863041

Strand: Direct

Name: ogt [H]

Synonym: CJE0923

Alternate gene names: 57237677

Gene position: 862589-863041 (Clockwise)

Preceding gene: 57237671

Following gene: 57237683

Centisome position: 48.52

GC content: 26.71

Gene sequence:

>453_bases
ATGTTTAAAGTTTATTATAAAATGCCTTTATGTTACCTATCTTTACATAGTGATGGAAAATTTTTAACTAGAGTCGATTT
TTGTGATAATAAAAGATCAGAAAAAAAATGTAGTTTGCTTGACTTAGCAAAATATGAACTTGATTTATATTTTACTCATA
AGCTTAGAAAATTTAGCATTCCTGTTTTGATACAAGGTACGGATTTTGAATCAAAGGTTTATAAGGCTTTAATGGAAATT
CCTTATGGCAAAATAGCTACTTATAAAGATATAGCAGAAAAAATTAATTATCCAAAAGCTTTTAGAGCTGTTGGAAATGC
AAATTCTAAAAATCAAATTCCTATTTTTATACCTTGTCATAGAGTTATTGCAAGCAATGGTATAGGAGGATATAATGGAG
GGCTTGAAATAAAAAGATTTTTGCTTGAAAATGAAGGAGTGAATTTAAAATAA

Upstream 100 bases:

>100_bases
CGGCTCTTTCTTCAACAAGTTTATTGTATTCTTGTATAAAAGACATTTTCTACTCCTTAAATATTTTTTATTATAATTGT
AGCTCTTTGAAGGATAAAAA

Downstream 100 bases:

>100_bases
GCTTGGGGTTTGATTTTCTTTGTTTTCAAATTCTTGTTCAAAAATATCATAATTTTCAAGAATTAAACAATATTTAAAAT
TTTTTAATTTTCTTAAATCT

Product: methylated-DNA--protein-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MFKVYYKMPLCYLSLHSDGKFLTRVDFCDNKRSEKKCSLLDLAKYELDLYFTHKLRKFSIPVLIQGTDFESKVYKALMEI
PYGKIATYKDIAEKINYPKAFRAVGNANSKNQIPIFIPCHRVIASNGIGGYNGGLEIKRFLLENEGVNLK

Sequences:

>Translated_150_residues
MFKVYYKMPLCYLSLHSDGKFLTRVDFCDNKRSEKKCSLLDLAKYELDLYFTHKLRKFSIPVLIQGTDFESKVYKALMEI
PYGKIATYKDIAEKINYPKAFRAVGNANSKNQIPIFIPCHRVIASNGIGGYNGGLEIKRFLLENEGVNLK
>Mature_150_residues
MFKVYYKMPLCYLSLHSDGKFLTRVDFCDNKRSEKKCSLLDLAKYELDLYFTHKLRKFSIPVLIQGTDFESKVYKALMEI
PYGKIATYKDIAEKINYPKAFRAVGNANSKNQIPIFIPCHRVIASNGIGGYNGGLEIKRFLLENEGVNLK

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=111, Percent_Identity=39.6396396396396, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1787596, Length=159, Percent_Identity=36.4779874213836, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1788542, Length=113, Percent_Identity=37.1681415929204, Blast_Score=84, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI115533068, Length=85, Percent_Identity=44.7058823529412, Blast_Score=79, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=85, Percent_Identity=44.7058823529412, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320001, Length=84, Percent_Identity=41.6666666666667, Blast_Score=78, Evalue=7e-16,
Organism=Drosophila melanogaster, GI17137554, Length=97, Percent_Identity=41.2371134020619, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17243; Mature: 17243

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKVYYKMPLCYLSLHSDGKFLTRVDFCDNKRSEKKCSLLDLAKYELDLYFTHKLRKFSI
CEEEEEECCEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHEEEEEEEECHHHCCC
PVLIQGTDFESKVYKALMEIPYGKIATYKDIAEKINYPKAFRAVGNANSKNQIPIFIPCH
CEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEEECH
RVIASNGIGGYNGGLEIKRFLLENEGVNLK
HHHHCCCCCCCCCCHHHHHEEHHCCCCCCC
>Mature Secondary Structure
MFKVYYKMPLCYLSLHSDGKFLTRVDFCDNKRSEKKCSLLDLAKYELDLYFTHKLRKFSI
CEEEEEECCEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHEEEEEEEECHHHCCC
PVLIQGTDFESKVYKALMEIPYGKIATYKDIAEKINYPKAFRAVGNANSKNQIPIFIPCH
CEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEEECH
RVIASNGIGGYNGGLEIKRFLLENEGVNLK
HHHHCCCCCCCCCCHHHHHEEHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA