Click here to switch to the map view.

The map label for this gene is napB [H]

Identifier: 57237628

GI number: 57237628

Start: 814948

End: 815469

Strand: Direct

Name: napB [H]

Synonym: CJE0874

Alternate gene names: 57237628

Gene position: 814948-815469 (Clockwise)

Preceding gene: 57237627

Following gene: 57237629

Centisome position: 45.84

GC content: 34.67

Gene sequence:

>522_bases
ATGAAGAAGAAATTGGTTTTATTAGGAAGTGCTGCAGTGGTATTTTTTGCCGCTTGTGCAATGAATAGTGGGGTAAGTTC
GGAACAAATTGGACTTAGAAAAGCAAGTTTAGAAAATGAAAATAAAGTAAATTTAGTGGAAGCAAATTTCACAACTTTAC
AACCTGGGGAATCTACTCGTTTTGAGCGTTCTTATGAAAATGCACCACCATTAATTCCGCATGCTATTGAAGATTTGTTA
CCTATAACTAAAGATAACAATATGTGCTTAAGCTGCCATGATAAGGCTATAGCAGCAGATGCTGGTGCAACTCCACTTCC
TGCTAGTCATTATTATGATTTTAGACACAATAAAACCACAGGAGATGTGATTAGCGATAGTCGTTTTAATTGCACTCAGT
GTCATGTTCCACAAAGTGATGCAAAACCTTTAGTGGGAAATAGCTTTAAACCTGAATTTAAAAATGAACAATTAAAAAGT
CGTTCAAATTTAATTGATGTGATTAATGAGGGTGTAAAGTAA

Upstream 100 bases:

>100_bases
CTAGCGTGAAATCAGGAGAGTGTATAAGGTGTGCAAGATGTATAGAAGTTTGTAATGATGATGCTTTGAATTTTAATATT
TTTGATTTAAGGAATAAATA

Downstream 100 bases:

>100_bases
ATAAATGAAAAAATTTCTTTTTATTTTGAGTCTTTTTTGTGTTTTGTCTTATGCTTATGAGCTGAAATTAAATGCCAATA
TAACAGCTTTAAAGCTTGAT

Product: periplasmic nitrate reductase, small subunit

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MKKKLVLLGSAAVVFFAACAMNSGVSSEQIGLRKASLENENKVNLVEANFTTLQPGESTRFERSYENAPPLIPHAIEDLL
PITKDNNMCLSCHDKAIAADAGATPLPASHYYDFRHNKTTGDVISDSRFNCTQCHVPQSDAKPLVGNSFKPEFKNEQLKS
RSNLIDVINEGVK

Sequences:

>Translated_173_residues
MKKKLVLLGSAAVVFFAACAMNSGVSSEQIGLRKASLENENKVNLVEANFTTLQPGESTRFERSYENAPPLIPHAIEDLL
PITKDNNMCLSCHDKAIAADAGATPLPASHYYDFRHNKTTGDVISDSRFNCTQCHVPQSDAKPLVGNSFKPEFKNEQLKS
RSNLIDVINEGVK
>Mature_173_residues
MKKKLVLLGSAAVVFFAACAMNSGVSSEQIGLRKASLENENKVNLVEANFTTLQPGESTRFERSYENAPPLIPHAIEDLL
PITKDNNMCLSCHDKAIAADAGATPLPASHYYDFRHNKTTGDVISDSRFNCTQCHVPQSDAKPLVGNSFKPEFKNEQLKS
RSNLIDVINEGVK

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=97, Percent_Identity=38.1443298969072, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: NA

Molecular weight: Translated: 18951; Mature: 18951

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKLVLLGSAAVVFFAACAMNSGVSSEQIGLRKASLENENKVNLVEANFTTLQPGESTR
CCCEEEEECHHHHHHHHHHHHCCCCCHHHHCHHHHCCCCCCCEEEEECCCEEECCCCCCC
FERSYENAPPLIPHAIEDLLPITKDNNMCLSCHDKAIAADAGATPLPASHYYDFRHNKTT
CHHCCCCCCCCCHHHHHHHCCCCCCCCEEEEECCCEEECCCCCCCCCCCCEEECCCCCCC
GDVISDSRFNCTQCHVPQSDAKPLVGNSFKPEFKNEQLKSRSNLIDVINEGVK
CCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKKLVLLGSAAVVFFAACAMNSGVSSEQIGLRKASLENENKVNLVEANFTTLQPGESTR
CCCEEEEECHHHHHHHHHHHHCCCCCHHHHCHHHHCCCCCCCEEEEECCCEEECCCCCCC
FERSYENAPPLIPHAIEDLLPITKDNNMCLSCHDKAIAADAGATPLPASHYYDFRHNKTT
CHHCCCCCCCCCHHHHHHHCCCCCCCCEEEEECCCEEECCCCCCCCCCCCEEECCCCCCC
GDVISDSRFNCTQCHVPQSDAKPLVGNSFKPEFKNEQLKSRSNLIDVINEGVK
CCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8376334; 12948488 [H]