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The map label for this gene is grpE

Identifier: 57237603

GI number: 57237603

Start: 787078

End: 787605

Strand: Direct

Name: grpE

Synonym: CJE0849

Alternate gene names: 57237603

Gene position: 787078-787605 (Clockwise)

Preceding gene: 57237602

Following gene: 57237604

Centisome position: 44.27

GC content: 31.63

Gene sequence:

>528_bases
ATGAGCGAACAAAAGCAAGAAATTGAAAATGAAAATGCACAAAATTCTGAAAATTTACAGGATGATTTGCAAGATAATGA
AAAAAACGAAACAAATGAATTACAAAAAGAATTAGAGGAATTAAAAGATAAATATATGCGTGCAAACGCTGAATTTGAAA
ATATTAAAAAAAGAATGGAAAAAGAAAAGCTAAGTGCTATGGCTTATGCAAATGAAAGCTTTGCAAAGGATTTGCTTGAT
GTTTTAGATGCTTTAGAAGCAGCTGTTAATGTCGAATGCCAAGATGAAATTAGCTTAAAAATCAAAGAAGGAGTGCAAAA
TACTTTAGATTTATTTCTTAAGAAACTTGAAAAACATGGGGTGGCTCTTATCAAAGATGAAAAAGAGTTTGATCCTAATT
TACACGAAGCAATGTTTCATGTAGATAGTGAAAACCATCAAAGTGGTGAAGTGGTTCAAGTGCTTCAAAAAGGTTATAAA
ATAGCCGATCGCGTGATCCGCCCAACCAAAGTTAGTGTAGCAAAATAA

Upstream 100 bases:

>100_bases
AGGTAGATGCGCAGTTTTTGGGCGAAGATGTAAATATTATCTTAGCAGGTAGTGTTTATACGGATTATAAAAAGATATTA
CAATACATAAAGGAGGCGGC

Downstream 100 bases:

>100_bases
ATTTTAATAAAAGGATAAAAAATGAGTAAAGTTATAGGTATAGATTTAGGAACAACCAATTCTTGTGTTGCTGTATATGA
ACGCGGCGAGAGCAAAGTAA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MSEQKQEIENENAQNSENLQDDLQDNEKNETNELQKELEELKDKYMRANAEFENIKKRMEKEKLSAMAYANESFAKDLLD
VLDALEAAVNVECQDEISLKIKEGVQNTLDLFLKKLEKHGVALIKDEKEFDPNLHEAMFHVDSENHQSGEVVQVLQKGYK
IADRVIRPTKVSVAK

Sequences:

>Translated_175_residues
MSEQKQEIENENAQNSENLQDDLQDNEKNETNELQKELEELKDKYMRANAEFENIKKRMEKEKLSAMAYANESFAKDLLD
VLDALEAAVNVECQDEISLKIKEGVQNTLDLFLKKLEKHGVALIKDEKEFDPNLHEAMFHVDSENHQSGEVVQVLQKGYK
IADRVIRPTKVSVAK
>Mature_174_residues
SEQKQEIENENAQNSENLQDDLQDNEKNETNELQKELEELKDKYMRANAEFENIKKRMEKEKLSAMAYANESFAKDLLDV
LDALEAAVNVECQDEISLKIKEGVQNTLDLFLKKLEKHGVALIKDEKEFDPNLHEAMFHVDSENHQSGEVVQVLQKGYKI
ADRVIRPTKVSVAK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=180, Percent_Identity=33.8888888888889, Blast_Score=84, Evalue=7e-17,
Organism=Homo sapiens, GI40255109, Length=153, Percent_Identity=37.2549019607843, Blast_Score=71, Evalue=5e-13,
Organism=Escherichia coli, GI1788967, Length=137, Percent_Identity=35.7664233576642, Blast_Score=83, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17552458, Length=153, Percent_Identity=37.9084967320261, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324806, Length=174, Percent_Identity=36.2068965517241, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24653432, Length=142, Percent_Identity=38.0281690140845, Blast_Score=87, Evalue=4e-18,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_CAMJR (Q5HV34)

Other databases:

- EMBL:   CP000025
- RefSeq:   YP_178851.1
- ProteinModelPortal:   Q5HV34
- SMR:   Q5HV34
- STRING:   Q5HV34
- GeneID:   3231362
- GenomeReviews:   CP000025_GR
- KEGG:   cjr:CJE0849
- TIGR:   CJE0849
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- ProtClustDB:   PRK14159
- BioCyc:   CJEJ195099:CJE_0849-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 20164; Mature: 20033

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQKQEIENENAQNSENLQDDLQDNEKNETNELQKELEELKDKYMRANAEFENIKKRME
CCHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KEKLSAMAYANESFAKDLLDVLDALEAAVNVECQDEISLKIKEGVQNTLDLFLKKLEKHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
VALIKDEKEFDPNLHEAMFHVDSENHQSGEVVQVLQKGYKIADRVIRPTKVSVAK
CEEEECCHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure 
SEQKQEIENENAQNSENLQDDLQDNEKNETNELQKELEELKDKYMRANAEFENIKKRME
CHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KEKLSAMAYANESFAKDLLDVLDALEAAVNVECQDEISLKIKEGVQNTLDLFLKKLEKHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
VALIKDEKEFDPNLHEAMFHVDSENHQSGEVVQVLQKGYKIADRVIRPTKVSVAK
CEEEECCHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA