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The map label for this gene is hypF [H]

Identifier: 57233776

GI number: 57233776

Start: 1302427

End: 1304718

Strand: Direct

Name: hypF [H]

Synonym: DET1432

Alternate gene names: 57233776

Gene position: 1302427-1304718 (Clockwise)

Preceding gene: 57233777

Following gene: 57233775

Centisome position: 88.62

GC content: 52.4

Gene sequence:

>2292_bases
ATGCCCGCACCTAAGCAAACCAAGCGGTTTGCCTTTGCCATCAGCGGGATTGTCCAGGGGGTGGGCTTCCGCCCCTTTGT
TTACCAGCTGGCCGCCAAACACGGCTTAAACGGCTGGGTCAGGAATACTTCTGCCGGAGTGGAGATAGAGGCAGAAGGGA
TTGAACAAGCGCTGGACAGCTTTTCAGATGAGCTAATTAGCCTCAGCCCGCCCCAGTCAGTTATTAAAACATTTAAACAG
ACTGAAATACCCTTAACAGGTGAAGAAGGGTTTACCATCAAACCCAGCCAGAGCCTTTCCGGGCGTTTTCAGCTGGTTTC
ACCGGATATAGCCACCTGCACCCAGTGCCAAAATGATGTATCTGACCCCCGAAACCGCCGCTACCGTTACCCCTTTACCA
ACTGCACCAACTGCGGCCCCCGTTTTACCATAATTGAGGACATACCCTATGACAGACCCCTGACCACTATGAAAGACTTT
GAAATGTGCCCGGTCTGCCAGGCCGAATATGACAACCCTTTAGACCGCCGTTTCCATGCCCAGCCCAATGCCTGCCCGGT
GTGCGGCCCCCGCCTCTGGCTGGTGGATAAAACCGGAAAAGAGCTTGAAAGTGCCGATGCTATAGCAGAAGCCGCAAAAC
TGCTGAAAGACGGCCGGATTTTAGCCATACGGGGTCTGGGGGGCTTTCTGCTGGCCGCTGACGCCACTAATCAGCTGGCG
GTTAATACCCTCCGGCGCCGTAAACACCGCCCTTCCAAACCCTTTGCGGTTATGCTTCCTGATATAGCCGAAGCCCGCAA
ACACTGTTTTATATCCGCCGAAGAGGAAAAGCTGCTTATTTCACCTGCCGCACCCATTGTCCTTTGCAAATGGAACAGGG
AAAGCCCCATATGCCCCGAAACCGCCCCTAAACAAAATTATCTGGGCATAATGCTGGCATACACCCCTCTCCACCACCTG
CTTTTAAAGGAAACGGGTAAACCCCTTATCATGACCAGCGGCAATCTGAGCGCAGAGCCCATTGCCAAAGACAATGACGA
AGCCCTGAAACGGCTGGGCAATATAGCGGATTATTTTCTGCTTCACAACCGCCCCATCTACTCCCGTTATGATGACAGCG
TGGTCATGTATGAGGCCGGTGCAAAAAGGGTAATCAGGCGGGGCAGGGGTTATGCCCCCTACCCTGTCCAGACCTCTTTT
ACGGATAAAGAAGTACTGGCGCTTGGTCCGCAGGAAAAAAACACCTTCTGCCTGCTTAAAGACGGTTATGCTTTTGTCAG
CCAGCATATAGGGGATATGCAAAACCCCGAAACGCTGGACAGCTTTGAAGAAACCCTGGCGGTTTACCTGAAGCTATTCC
GCTTAAACCCCCAAATAATAGCTTCAGACCTCCACCCCGGTTATATTACCACCGCTCTGGCTGAGGCACGCTCAGCCAGC
CTTGACATACCTCTGGTCAAAGTCCAGCACCACCATGCCCATATTGCCTCCTGTCTGGCGGAAAACAACTGTGACGAAGA
GGTTATCGGGGTGGCTCTGGACGGCACCGGCTACGGGCTGGACGGGCATATCTGGGGCGGGGAATTTTTCTGCGGGGGCA
TAAAACAGGGTTTTAGCCGCCAAGCCCATCTGGAATACCTGCCTCTCCCCGGAGGCGAAGCCGCCATTAAAAAACCCTAC
CGCACAGCCGTGTCATATGTCTACACCCTGCTGGGGGAAGAGGGTTTGAACTTTTTTAAAAATATAGAACCGGAAGAACT
GGATATCATAAAAACCCAGCTTGAGCGGAAACTGAACTCACCCCTTACTTCCAGTGCGGGCAGGCTGTTTGATGCAGTTG
CCGCCCTGTGCGGAATCTGCCAAGTGGCAAACTATGATTCCCAGCCGGCCATAGAGTTGGAAACCGAAGCCGGGGATACC
GAATTTGCCCAGTGCTACCCCTTTGAGTTAGACTTAGACAAGGGCATTTACCTGATACGGCTGAAGGGCATAATCCAAGG
CGTCCTTTCAGACCTTTCATCAGGCAAAACAGCCGCTTATATATCGGTAAAATTTCACAATACTGTCAGCCAGATAGTTC
TGGACACCTGCCTAAGGCTTTCAGCCCGAAGCGGCCTTACCAAAGTAGCCCTTTCAGGCGGGGTTTTCCAGAACAGGCGG
GTTTTCCGCCAGGTGATAAAAAAGCTGGAAGCAGAGGGATTTGAAGTGATTACCCATAAAGAAATCCCCTCTAATGACGG
CTGCATATCACTGGGTCAGGCGGTAATAGCCACCAGCCAAGGAGGAATATAA

Upstream 100 bases:

>100_bases
CAACCCGGACGTAAAAATAATTGAGCTTTCAGTAAGGAATGGCCAGGGGCTGGACGAATGGACAGCCTGGCTGAAAAGCA
ACCTCCTCAAGGGGAAATAG

Downstream 100 bases:

>100_bases
TGTGCCTTGCCGTACCTGCCAAAATAATATCAGTTGAAGACGGGGTAGCGGAGGTAGACCTGAACGGGACTACCTATAAA
GCCAGCCTGATGCTCACCCC

Product: [NiFe] hydrogenase maturation protein HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 763; Mature: 762

Protein sequence:

>763_residues
MPAPKQTKRFAFAISGIVQGVGFRPFVYQLAAKHGLNGWVRNTSAGVEIEAEGIEQALDSFSDELISLSPPQSVIKTFKQ
TEIPLTGEEGFTIKPSQSLSGRFQLVSPDIATCTQCQNDVSDPRNRRYRYPFTNCTNCGPRFTIIEDIPYDRPLTTMKDF
EMCPVCQAEYDNPLDRRFHAQPNACPVCGPRLWLVDKTGKELESADAIAEAAKLLKDGRILAIRGLGGFLLAADATNQLA
VNTLRRRKHRPSKPFAVMLPDIAEARKHCFISAEEEKLLISPAAPIVLCKWNRESPICPETAPKQNYLGIMLAYTPLHHL
LLKETGKPLIMTSGNLSAEPIAKDNDEALKRLGNIADYFLLHNRPIYSRYDDSVVMYEAGAKRVIRRGRGYAPYPVQTSF
TDKEVLALGPQEKNTFCLLKDGYAFVSQHIGDMQNPETLDSFEETLAVYLKLFRLNPQIIASDLHPGYITTALAEARSAS
LDIPLVKVQHHHAHIASCLAENNCDEEVIGVALDGTGYGLDGHIWGGEFFCGGIKQGFSRQAHLEYLPLPGGEAAIKKPY
RTAVSYVYTLLGEEGLNFFKNIEPEELDIIKTQLERKLNSPLTSSAGRLFDAVAALCGICQVANYDSQPAIELETEAGDT
EFAQCYPFELDLDKGIYLIRLKGIIQGVLSDLSSGKTAAYISVKFHNTVSQIVLDTCLRLSARSGLTKVALSGGVFQNRR
VFRQVIKKLEAEGFEVITHKEIPSNDGCISLGQAVIATSQGGI

Sequences:

>Translated_763_residues
MPAPKQTKRFAFAISGIVQGVGFRPFVYQLAAKHGLNGWVRNTSAGVEIEAEGIEQALDSFSDELISLSPPQSVIKTFKQ
TEIPLTGEEGFTIKPSQSLSGRFQLVSPDIATCTQCQNDVSDPRNRRYRYPFTNCTNCGPRFTIIEDIPYDRPLTTMKDF
EMCPVCQAEYDNPLDRRFHAQPNACPVCGPRLWLVDKTGKELESADAIAEAAKLLKDGRILAIRGLGGFLLAADATNQLA
VNTLRRRKHRPSKPFAVMLPDIAEARKHCFISAEEEKLLISPAAPIVLCKWNRESPICPETAPKQNYLGIMLAYTPLHHL
LLKETGKPLIMTSGNLSAEPIAKDNDEALKRLGNIADYFLLHNRPIYSRYDDSVVMYEAGAKRVIRRGRGYAPYPVQTSF
TDKEVLALGPQEKNTFCLLKDGYAFVSQHIGDMQNPETLDSFEETLAVYLKLFRLNPQIIASDLHPGYITTALAEARSAS
LDIPLVKVQHHHAHIASCLAENNCDEEVIGVALDGTGYGLDGHIWGGEFFCGGIKQGFSRQAHLEYLPLPGGEAAIKKPY
RTAVSYVYTLLGEEGLNFFKNIEPEELDIIKTQLERKLNSPLTSSAGRLFDAVAALCGICQVANYDSQPAIELETEAGDT
EFAQCYPFELDLDKGIYLIRLKGIIQGVLSDLSSGKTAAYISVKFHNTVSQIVLDTCLRLSARSGLTKVALSGGVFQNRR
VFRQVIKKLEAEGFEVITHKEIPSNDGCISLGQAVIATSQGGI
>Mature_762_residues
PAPKQTKRFAFAISGIVQGVGFRPFVYQLAAKHGLNGWVRNTSAGVEIEAEGIEQALDSFSDELISLSPPQSVIKTFKQT
EIPLTGEEGFTIKPSQSLSGRFQLVSPDIATCTQCQNDVSDPRNRRYRYPFTNCTNCGPRFTIIEDIPYDRPLTTMKDFE
MCPVCQAEYDNPLDRRFHAQPNACPVCGPRLWLVDKTGKELESADAIAEAAKLLKDGRILAIRGLGGFLLAADATNQLAV
NTLRRRKHRPSKPFAVMLPDIAEARKHCFISAEEEKLLISPAAPIVLCKWNRESPICPETAPKQNYLGIMLAYTPLHHLL
LKETGKPLIMTSGNLSAEPIAKDNDEALKRLGNIADYFLLHNRPIYSRYDDSVVMYEAGAKRVIRRGRGYAPYPVQTSFT
DKEVLALGPQEKNTFCLLKDGYAFVSQHIGDMQNPETLDSFEETLAVYLKLFRLNPQIIASDLHPGYITTALAEARSASL
DIPLVKVQHHHAHIASCLAENNCDEEVIGVALDGTGYGLDGHIWGGEFFCGGIKQGFSRQAHLEYLPLPGGEAAIKKPYR
TAVSYVYTLLGEEGLNFFKNIEPEELDIIKTQLERKLNSPLTSSAGRLFDAVAALCGICQVANYDSQPAIELETEAGDTE
FAQCYPFELDLDKGIYLIRLKGIIQGVLSDLSSGKTAAYISVKFHNTVSQIVLDTCLRLSARSGLTKVALSGGVFQNRRV
FRQVIKKLEAEGFEVITHKEIPSNDGCISLGQAVIATSQGGI

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=762, Percent_Identity=39.3700787401575, Blast_Score=440, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 84121; Mature: 83990

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPKQTKRFAFAISGIVQGVGFRPFVYQLAAKHGLNGWVRNTSAGVEIEAEGIEQALDS
CCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEECCCCCCEEEHHHHHHHHHH
FSDELISLSPPQSVIKTFKQTEIPLTGEEGFTIKPSQSLSGRFQLVSPDIATCTQCQNDV
HHHHHEECCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCCEEEEECCCHHHHHHHHHHC
SDPRNRRYRYPFTNCTNCGPRFTIIEDIPYDRPLTTMKDFEMCPVCQAEYDNPLDRRFHA
CCHHHCCEECCCCCCCCCCCCEEEEECCCCCCCCCCHHCCCCCCCCHHCCCCCHHHHHCC
QPNACPVCGPRLWLVDKTGKELESADAIAEAAKLLKDGRILAIRGLGGFLLAADATNQLA
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCHHH
VNTLRRRKHRPSKPFAVMLPDIAEARKHCFISAEEEKLLISPAAPIVLCKWNRESPICPE
HHHHHHHCCCCCCCEEEECCCHHHHHHHEEEECCCCEEEEECCCCEEEEEECCCCCCCCC
TAPKQNYLGIMLAYTPLHHLLLKETGKPLIMTSGNLSAEPIAKDNDEALKRLGNIADYFL
CCCCCCCCEEEEHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
LHNRPIYSRYDDSVVMYEAGAKRVIRRGRGYAPYPVQTSFTDKEVLALGPQEKNTFCLLK
CCCCCCCCCCCCCEEEEECCHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEE
DGYAFVSQHIGDMQNPETLDSFEETLAVYLKLFRLNPQIIASDLHPGYITTALAEARSAS
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCHHHHHHHHHHCCC
LDIPLVKVQHHHAHIASCLAENNCDEEVIGVALDGTGYGLDGHIWGGEFFCGGIKQGFSR
CCCEEEEEEHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEECCCEEECHHHHCCCC
QAHLEYLPLPGGEAAIKKPYRTAVSYVYTLLGEEGLNFFKNIEPEELDIIKTQLERKLNS
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
PLTSSAGRLFDAVAALCGICQVANYDSQPAIELETEAGDTEFAQCYPFELDLDKGIYLIR
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEECEEEECCCCEEEEE
LKGIIQGVLSDLSSGKTAAYISVKFHNTVSQIVLDTCLRLSARSGLTKVALSGGVFQNRR
HHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHH
VFRQVIKKLEAEGFEVITHKEIPSNDGCISLGQAVIATSQGGI
HHHHHHHHHCCCCEEEEEECCCCCCCCCHHHCCEEEEECCCCC
>Mature Secondary Structure 
PAPKQTKRFAFAISGIVQGVGFRPFVYQLAAKHGLNGWVRNTSAGVEIEAEGIEQALDS
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEECCCCCCEEEHHHHHHHHHH
FSDELISLSPPQSVIKTFKQTEIPLTGEEGFTIKPSQSLSGRFQLVSPDIATCTQCQNDV
HHHHHEECCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCCEEEEECCCHHHHHHHHHHC
SDPRNRRYRYPFTNCTNCGPRFTIIEDIPYDRPLTTMKDFEMCPVCQAEYDNPLDRRFHA
CCHHHCCEECCCCCCCCCCCCEEEEECCCCCCCCCCHHCCCCCCCCHHCCCCCHHHHHCC
QPNACPVCGPRLWLVDKTGKELESADAIAEAAKLLKDGRILAIRGLGGFLLAADATNQLA
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCHHH
VNTLRRRKHRPSKPFAVMLPDIAEARKHCFISAEEEKLLISPAAPIVLCKWNRESPICPE
HHHHHHHCCCCCCCEEEECCCHHHHHHHEEEECCCCEEEEECCCCEEEEEECCCCCCCCC
TAPKQNYLGIMLAYTPLHHLLLKETGKPLIMTSGNLSAEPIAKDNDEALKRLGNIADYFL
CCCCCCCCEEEEHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
LHNRPIYSRYDDSVVMYEAGAKRVIRRGRGYAPYPVQTSFTDKEVLALGPQEKNTFCLLK
CCCCCCCCCCCCCEEEEECCHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEE
DGYAFVSQHIGDMQNPETLDSFEETLAVYLKLFRLNPQIIASDLHPGYITTALAEARSAS
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCHHHHHHHHHHCCC
LDIPLVKVQHHHAHIASCLAENNCDEEVIGVALDGTGYGLDGHIWGGEFFCGGIKQGFSR
CCCEEEEEEHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEECCCEEECHHHHCCCC
QAHLEYLPLPGGEAAIKKPYRTAVSYVYTLLGEEGLNFFKNIEPEELDIIKTQLERKLNS
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
PLTSSAGRLFDAVAALCGICQVANYDSQPAIELETEAGDTEFAQCYPFELDLDKGIYLIR
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEECEEEECCCCEEEEE
LKGIIQGVLSDLSSGKTAAYISVKFHNTVSQIVLDTCLRLSARSGLTKVALSGGVFQNRR
HHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHH
VFRQVIKKLEAEGFEVITHKEIPSNDGCISLGQAVIATSQGGI
HHHHHHHHHCCCCEEEEEECCCCCCCCCHHHCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]