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The map label for this gene is cca [H]

Identifier: 55980894

GI number: 55980894

Start: 875988

End: 877283

Strand: Reverse

Name: cca [H]

Synonym: TTHA0925

Alternate gene names: 55980894

Gene position: 877283-875988 (Counterclockwise)

Preceding gene: 55980896

Following gene: 55980893

Centisome position: 47.43

GC content: 71.06

Gene sequence:

>1296_bases
ATGGACTTCCCCTTTTATACCCCCAAGGACGCCTTCCCCGTGGGCGGGGCGGTGCGGGACCTCCTCCTGGGAAGAAGGCC
AACGGACCTGGACTACGCCGCCTTGGACCCCGAAGGGGCGGCCGAGGAGGCAAAGAGGCGCCTCGGGGGAAGCCTCTTCC
CCCTGGACCCCAAGCGGGGCCACTACCGCCTGGTGGTGGGGGAAAGGACCCTGGACTTCACCCCCTTGGAGGGGAGGCTG
GAGGAGGACCTCCTCCGGCGGGACTACCGGGTGAACGCCCTCCTCTGGAAGGGGGGCGCCGTCTTCGGCCTAAAGGGGGT
GGAGGAGGACCTGAGGCGGCGCCTCCTCGTCCCCGTGCGGGAGGAAAACCTCTACCAGGACCACCTGCGAAGCCTCCGGG
GGGTCCGCCTCGCCGCCACCTTGGGCTTCGGGCTTCCCCAGAGGACCCGGGAGGCCCTTGGCCGCCACGCCCGGTTCCTC
CAGGCCCACCCCGAGGCCCTCCCCGCGCGGGAAAGGGTCAAGGAGGAGCTTGCCCGGCTCCTCCTCTCCCCGAGGGCGGC
CTTCGGCCTGCGCCTTCTGGAGAGGGTGGGGCTCCTTGGGGTCTACCTCCCCGAGCTCGCCCTCCTCGTGGGCCTCCATC
AGGGCGGGGTGCACCACCTTCCCGCCTGGGACCACACCCTGAGCGCGGTCTTCCACCTCCTCTGGCTCTGGCCCGAGGCC
CCCCTCGAGGCCCGCCTCGCCGCCCTCTTCCACGACGTGGGCAAGCCCCTCACCCGCCGCTTTGACCCCGAGGTGGGCCG
CTTCCGCTTCCTGGGGCACGCCGAGGTGGGGGCGGAGATCGCCCGGGCAAGCCTCTTTTGGCTCCGCTTCCCCAAGGAGG
TGGTGGAGCGGGCGGCGGGCCTGGTGCGCCGCCACATGGACCGCCTCCCCGAGGAGAGGAAGGCCCTGCGCCGCTTCTTC
CTGAGGCGCCAGGACCTCCTCCCGGACCTCGTCTACCTCATGGCGGCGGACCGCCTGGCCACGAGGGGCGTGGAGCGCGA
GGCCTGGGAGGTGCTCGGGCGCTACGAAGAGGTCCTGAAAGACCCCCTGCCGCAAAGGCCCCTCCTCTCCGGGGAGGAGG
TGATGGCCCTTCTGGGCCTCCAAGAAGGCCCCGAGGTGGGAAGGGCCCTAAAGGCCCTCCTCGAGGCCCAGGCCGAAGGC
CGGGTGGGGACCAAGGAGGAGGCCCGGGCCTTTCTCCTATATTGGAGGGGTGGAAGGGAGGCTCAGGCTTCGGGAACCCC
AGATCACCCCCATTGA

Upstream 100 bases:

>100_bases
GGTAGCCCCAGGACCTGAGGAGGGCGGCCACGGTGCTCTTGCCGCTGCCGATGTTCCCGGTGATGCCGATAATAATGGGG
TGCTTCGCCTCGTGGCCCAT

Downstream 100 bases:

>100_bases
GGGGGGCTTCCTGGTCTCCGACCCCTACGGGGTCTACGAGAAGCCCCTGGCCCTCACCGAGGGCGGGCTTTTCCTCCTCT
CCCTCATGGAGGGGAGGACC

Product: poly(A) polymerase family protein

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MDFPFYTPKDAFPVGGAVRDLLLGRRPTDLDYAALDPEGAAEEAKRRLGGSLFPLDPKRGHYRLVVGERTLDFTPLEGRL
EEDLLRRDYRVNALLWKGGAVFGLKGVEEDLRRRLLVPVREENLYQDHLRSLRGVRLAATLGFGLPQRTREALGRHARFL
QAHPEALPARERVKEELARLLLSPRAAFGLRLLERVGLLGVYLPELALLVGLHQGGVHHLPAWDHTLSAVFHLLWLWPEA
PLEARLAALFHDVGKPLTRRFDPEVGRFRFLGHAEVGAEIARASLFWLRFPKEVVERAAGLVRRHMDRLPEERKALRRFF
LRRQDLLPDLVYLMAADRLATRGVEREAWEVLGRYEEVLKDPLPQRPLLSGEEVMALLGLQEGPEVGRALKALLEAQAEG
RVGTKEEARAFLLYWRGGREAQASGTPDHPH

Sequences:

>Translated_431_residues
MDFPFYTPKDAFPVGGAVRDLLLGRRPTDLDYAALDPEGAAEEAKRRLGGSLFPLDPKRGHYRLVVGERTLDFTPLEGRL
EEDLLRRDYRVNALLWKGGAVFGLKGVEEDLRRRLLVPVREENLYQDHLRSLRGVRLAATLGFGLPQRTREALGRHARFL
QAHPEALPARERVKEELARLLLSPRAAFGLRLLERVGLLGVYLPELALLVGLHQGGVHHLPAWDHTLSAVFHLLWLWPEA
PLEARLAALFHDVGKPLTRRFDPEVGRFRFLGHAEVGAEIARASLFWLRFPKEVVERAAGLVRRHMDRLPEERKALRRFF
LRRQDLLPDLVYLMAADRLATRGVEREAWEVLGRYEEVLKDPLPQRPLLSGEEVMALLGLQEGPEVGRALKALLEAQAEG
RVGTKEEARAFLLYWRGGREAQASGTPDHPH
>Mature_431_residues
MDFPFYTPKDAFPVGGAVRDLLLGRRPTDLDYAALDPEGAAEEAKRRLGGSLFPLDPKRGHYRLVVGERTLDFTPLEGRL
EEDLLRRDYRVNALLWKGGAVFGLKGVEEDLRRRLLVPVREENLYQDHLRSLRGVRLAATLGFGLPQRTREALGRHARFL
QAHPEALPARERVKEELARLLLSPRAAFGLRLLERVGLLGVYLPELALLVGLHQGGVHHLPAWDHTLSAVFHLLWLWPEA
PLEARLAALFHDVGKPLTRRFDPEVGRFRFLGHAEVGAEIARASLFWLRFPKEVVERAAGLVRRHMDRLPEERKALRRFF
LRRQDLLPDLVYLMAADRLATRGVEREAWEVLGRYEEVLKDPLPQRPLLSGEEVMALLGLQEGPEVGRALKALLEAQAEG
RVGTKEEARAFLLYWRGGREAQASGTPDHPH

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081691, Length=231, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1789436, Length=301, Percent_Identity=31.5614617940199, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 48889; Mature: 48889

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFPFYTPKDAFPVGGAVRDLLLGRRPTDLDYAALDPEGAAEEAKRRLGGSLFPLDPKRG
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
HYRLVVGERTLDFTPLEGRLEEDLLRRDYRVNALLWKGGAVFGLKGVEEDLRRRLLVPVR
CEEEEEECCCCCCCCCCCHHHHHHHHHCHHCEEEEECCCCEEECCCHHHHHHHHHCCCCH
EENLYQDHLRSLRGVRLAATLGFGLPQRTREALGRHARFLQAHPEALPARERVKEELARL
HHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LLSPRAAFGLRLLERVGLLGVYLPELALLVGLHQGGVHHLPAWDHTLSAVFHLLWLWPEA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
PLEARLAALFHDVGKPLTRRFDPEVGRFRFLGHAEVGAEIARASLFWLRFPKEVVERAAG
CHHHHHHHHHHHHCHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHH
LVRRHMDRLPEERKALRRFFLRRQDLLPDLVYLMAADRLATRGVEREAWEVLGRYEEVLK
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
DPLPQRPLLSGEEVMALLGLQEGPEVGRALKALLEAQAEGRVGTKEEARAFLLYWRGGRE
CCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCEEEEEEECCCC
AQASGTPDHPH
CCCCCCCCCCC
>Mature Secondary Structure
MDFPFYTPKDAFPVGGAVRDLLLGRRPTDLDYAALDPEGAAEEAKRRLGGSLFPLDPKRG
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
HYRLVVGERTLDFTPLEGRLEEDLLRRDYRVNALLWKGGAVFGLKGVEEDLRRRLLVPVR
CEEEEEECCCCCCCCCCCHHHHHHHHHCHHCEEEEECCCCEEECCCHHHHHHHHHCCCCH
EENLYQDHLRSLRGVRLAATLGFGLPQRTREALGRHARFLQAHPEALPARERVKEELARL
HHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LLSPRAAFGLRLLERVGLLGVYLPELALLVGLHQGGVHHLPAWDHTLSAVFHLLWLWPEA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
PLEARLAALFHDVGKPLTRRFDPEVGRFRFLGHAEVGAEIARASLFWLRFPKEVVERAAG
CHHHHHHHHHHHHCHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHH
LVRRHMDRLPEERKALRRFFLRRQDLLPDLVYLMAADRLATRGVEREAWEVLGRYEEVLK
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
DPLPQRPLLSGEEVMALLGLQEGPEVGRALKALLEAQAEGRVGTKEEARAFLLYWRGGRE
CCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCEEEEEEECCCC
AQASGTPDHPH
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA