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The map label for this gene is nfo [H]

Identifier: 55980803

GI number: 55980803

Start: 799876

End: 800688

Strand: Reverse

Name: nfo [H]

Synonym: TTHA0834

Alternate gene names: 55980803

Gene position: 800688-799876 (Counterclockwise)

Preceding gene: 55980804

Following gene: 55980799

Centisome position: 43.29

GC content: 71.09

Gene sequence:

>813_bases
ATGCCGCGCTACGGGTTCCACCTTTCCATCGCCGGGAAAAAGGGCGTGGCCGGGGCGGTGGAGGAAGCCACCGCCCTCGG
CCTCACCGCTTTCCAGATCTTCGCCAAAAGCCCGCGGAGCTGGCGCCCAAGGGCCCTCTCCCCGGCCGAGGTGGAGGCCT
TCCGCGCCTTAAGGGAGGCCTCCGGGGGCCTCCCCGCCGTGATCCACGCCTCCTACCTGGTCAACCTGGGGGCGGAGGGG
GAGCTTTGGGAGAAGAGCGTGGCGAGCCTGGCGGACGACCTGGAGAAGGCCGCCCTCCTCGGGGTGGAGTACGTGGTCGT
CCACCCCGGCTCGGGCCGCCCCGAGCGGGTCAAGGAAGGGGCCCTCAAGGCCCTGCGCCTCGCCGGCGTCCGCTCCCGCC
CCGTCCTCCTCGTGGAGAACACCGCTGGGGGTGGGGAGAAGGTGGGGGCGCGGTTTGAGGAGCTCGCCTGGCTCGTGGCG
GACACCCCCCTCCAGGTCTGCCTGGACACCTGCCACGCCTACGCCGCCGGGTACGACGTGGCCGAGGACCCCTTGGGGGT
CCTGGACGCCTTGGACCGGGCCGTGGGCCTGGAGCGGGTGCCCGTGGTCCACCTCAACGACTCCGTGGGCGGCCTCGGAA
GCCGCGTGGACCACCACGCCCACCTCCTCCAGGGAAAGATCGGGGAGGGGCTCAAGCGCGTCTTTTTGGACCCGAGGCTC
AAGGACCGGGTCTTCATCCTGGAAACCCCCAGGGGACCGGAGGAGGACGCCTGGAACCTCCGGGTCTTTAGGGCCTGGCT
CGAGGAGGCCTAA

Upstream 100 bases:

>100_bases
GAGAGCCTGAAGCGGGGCTTCGCCCTCTTCCTGGTGGCCATGGGGGTCTTCATCGTGGCCCAGAACCTAGGCTAAGCGCC
CCTGTGGTAGGCTTCGGGGG

Downstream 100 bases:

>100_bases
GCGCCCCACCCTGGCCTTGGGCCGAAGGGGAGGCCCCTAAGTATCCCACCGCGGCTTTCGCCGCGGTGGGGGCCCCAAAT
TAGCTTCCCAAGCCTTATTC

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MPRYGFHLSIAGKKGVAGAVEEATALGLTAFQIFAKSPRSWRPRALSPAEVEAFRALREASGGLPAVIHASYLVNLGAEG
ELWEKSVASLADDLEKAALLGVEYVVVHPGSGRPERVKEGALKALRLAGVRSRPVLLVENTAGGGEKVGARFEELAWLVA
DTPLQVCLDTCHAYAAGYDVAEDPLGVLDALDRAVGLERVPVVHLNDSVGGLGSRVDHHAHLLQGKIGEGLKRVFLDPRL
KDRVFILETPRGPEEDAWNLRVFRAWLEEA

Sequences:

>Translated_270_residues
MPRYGFHLSIAGKKGVAGAVEEATALGLTAFQIFAKSPRSWRPRALSPAEVEAFRALREASGGLPAVIHASYLVNLGAEG
ELWEKSVASLADDLEKAALLGVEYVVVHPGSGRPERVKEGALKALRLAGVRSRPVLLVENTAGGGEKVGARFEELAWLVA
DTPLQVCLDTCHAYAAGYDVAEDPLGVLDALDRAVGLERVPVVHLNDSVGGLGSRVDHHAHLLQGKIGEGLKRVFLDPRL
KDRVFILETPRGPEEDAWNLRVFRAWLEEA
>Mature_269_residues
PRYGFHLSIAGKKGVAGAVEEATALGLTAFQIFAKSPRSWRPRALSPAEVEAFRALREASGGLPAVIHASYLVNLGAEGE
LWEKSVASLADDLEKAALLGVEYVVVHPGSGRPERVKEGALKALRLAGVRSRPVLLVENTAGGGEKVGARFEELAWLVAD
TPLQVCLDTCHAYAAGYDVAEDPLGVLDALDRAVGLERVPVVHLNDSVGGLGSRVDHHAHLLQGKIGEGLKRVFLDPRLK
DRVFILETPRGPEEDAWNLRVFRAWLEEA

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=284, Percent_Identity=35.2112676056338, Blast_Score=135, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17531193, Length=268, Percent_Identity=35.0746268656716, Blast_Score=148, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6322735, Length=284, Percent_Identity=30.6338028169014, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 29086; Mature: 28954

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRYGFHLSIAGKKGVAGAVEEATALGLTAFQIFAKSPRSWRPRALSPAEVEAFRALREA
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
SGGLPAVIHASYLVNLGAEGELWEKSVASLADDLEKAALLGVEYVVVHPGSGRPERVKEG
CCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHH
ALKALRLAGVRSRPVLLVENTAGGGEKVGARFEELAWLVADTPLQVCLDTCHAYAAGYDV
HHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
AEDPLGVLDALDRAVGLERVPVVHLNDSVGGLGSRVDHHAHLLQGKIGEGLKRVFLDPRL
CCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
KDRVFILETPRGPEEDAWNLRVFRAWLEEA
CCCEEEEECCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PRYGFHLSIAGKKGVAGAVEEATALGLTAFQIFAKSPRSWRPRALSPAEVEAFRALREA
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
SGGLPAVIHASYLVNLGAEGELWEKSVASLADDLEKAALLGVEYVVVHPGSGRPERVKEG
CCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHH
ALKALRLAGVRSRPVLLVENTAGGGEKVGARFEELAWLVADTPLQVCLDTCHAYAAGYDV
HHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
AEDPLGVLDALDRAVGLERVPVVHLNDSVGGLGSRVDHHAHLLQGKIGEGLKRVFLDPRL
CCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
KDRVFILETPRGPEEDAWNLRVFRAWLEEA
CCCEEEEECCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA