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The map label for this gene is tgt [H]

Identifier: 54295566

GI number: 54295566

Start: 3023860

End: 3025011

Strand: Reverse

Name: tgt [H]

Synonym: lpl2653

Alternate gene names: 54295566

Gene position: 3025011-3023860 (Counterclockwise)

Preceding gene: 54295567

Following gene: 54295565

Centisome position: 90.42

GC content: 40.62

Gene sequence:

>1152_bases
ATGAACACTTTTTCATGTGAAGTACTGTACCAGCATTCGGGTACCAAAGCTCGTGTTACACGTGTGGTTACGCCTCATGG
TGAATTTATAACCCCGGTATTTATGCCAGTTGGAACCCGTGCAGGGGTCAATAACATGATGCCGTCAGAACTGTCTGCTG
CGGGTACTCAAATTATTCTGGGCGGTAATACCTATCATATGTTGTGTGCGCCTGGCATGAAGGTGATAGAAAATGCAGGG
GGGATGCATCGCTTTATGGGGTGGCATGGACCTATGCTAACAGACAGCGGTGGTTTTCAAGTTTTTAGTCTATCGAAGAA
CAAGGAAATTTGTTCTATAGATGAATCTGGTGCTCATTTTAAATTGCCTGGCAGTAAGGGGATTATCCATATGACCCCGG
AAATGTCTCTGGAGACCCAGAAAATAATTGGTGCCGATATTATAATGGCATTTGATCAGTGTACTCCTGATAGCTGCTCC
AAGGATGAAGTAAGTCAAATCATGTTGCGGACCCACAGATGGCTAAAACAATCGATTGACTACCATCAACAATATCCCAA
CTCTCGATACGGATATAAACAAGCTTTATTTGGCATTGTGCAAGGAGGAGTTTTTCAAGATTTACGTCGAGAAAGTGCTG
ATTTTGTTTCTGCAATGAATACGGATGGTATTGCAATAGGTGGAGAAACTGTAGGCTTTGATATGGATACTACAGTGGAA
GTCATTCGTTGGGTGTATGAGTATCTTCCGGGCAATAAGCCACGTTATACTATGGGAGTTGGAATGTCACCTCAAGATCT
GTTGGATGTGGTGGCAGAAGGTATCGATATGTTTGATTGTGTTGCACCAACACGTAATGCCCGACATGGTGCCTTATATT
GTGGTAAATTGGTGAGAGAAGGGAGTTGGCTAAAATTTCAAAGCGAACATGAAAATGGACGAATTCAAATAAAGAAATCA
TGTTTTGCTGATGATTTGGCACCTGTTATGGAGAGTTGTTCTTGTTATACTTGTCTTAATTATTCAAGATCCTATTTGCA
TCATTTATCAAAACAAAAAGCTAATTTATTTACTGCTTTGGCAAGCATCCATAATGTTCATGTTTTGCATGATGTTTGTA
ATCACATGCGTCATTTGATAATAAATTCCTAA

Upstream 100 bases:

>100_bases
GGGCTTTTTCTGTTTTTTCGAACCCGAACGATCAATTAATTCCTGGCATTAAACGCGTGCTTTCGTTATCATTCTTTGTT
TTATCTTGTCGTTTCTGCGA

Downstream 100 bases:

>100_bases
ATTATTTAATATTAAGAGGTGAAATAATGGTTTTAATTTCTACATCCATGGGTGATATTCATATCAAATTAGATACAGAA
AATACCCCATTAACTGCAGA

Product: hypothetical protein

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MNTFSCEVLYQHSGTKARVTRVVTPHGEFITPVFMPVGTRAGVNNMMPSELSAAGTQIILGGNTYHMLCAPGMKVIENAG
GMHRFMGWHGPMLTDSGGFQVFSLSKNKEICSIDESGAHFKLPGSKGIIHMTPEMSLETQKIIGADIIMAFDQCTPDSCS
KDEVSQIMLRTHRWLKQSIDYHQQYPNSRYGYKQALFGIVQGGVFQDLRRESADFVSAMNTDGIAIGGETVGFDMDTTVE
VIRWVYEYLPGNKPRYTMGVGMSPQDLLDVVAEGIDMFDCVAPTRNARHGALYCGKLVREGSWLKFQSEHENGRIQIKKS
CFADDLAPVMESCSCYTCLNYSRSYLHHLSKQKANLFTALASIHNVHVLHDVCNHMRHLIINS

Sequences:

>Translated_383_residues
MNTFSCEVLYQHSGTKARVTRVVTPHGEFITPVFMPVGTRAGVNNMMPSELSAAGTQIILGGNTYHMLCAPGMKVIENAG
GMHRFMGWHGPMLTDSGGFQVFSLSKNKEICSIDESGAHFKLPGSKGIIHMTPEMSLETQKIIGADIIMAFDQCTPDSCS
KDEVSQIMLRTHRWLKQSIDYHQQYPNSRYGYKQALFGIVQGGVFQDLRRESADFVSAMNTDGIAIGGETVGFDMDTTVE
VIRWVYEYLPGNKPRYTMGVGMSPQDLLDVVAEGIDMFDCVAPTRNARHGALYCGKLVREGSWLKFQSEHENGRIQIKKS
CFADDLAPVMESCSCYTCLNYSRSYLHHLSKQKANLFTALASIHNVHVLHDVCNHMRHLIINS
>Mature_383_residues
MNTFSCEVLYQHSGTKARVTRVVTPHGEFITPVFMPVGTRAGVNNMMPSELSAAGTQIILGGNTYHMLCAPGMKVIENAG
GMHRFMGWHGPMLTDSGGFQVFSLSKNKEICSIDESGAHFKLPGSKGIIHMTPEMSLETQKIIGADIIMAFDQCTPDSCS
KDEVSQIMLRTHRWLKQSIDYHQQYPNSRYGYKQALFGIVQGGVFQDLRRESADFVSAMNTDGIAIGGETVGFDMDTTVE
VIRWVYEYLPGNKPRYTMGVGMSPQDLLDVVAEGIDMFDCVAPTRNARHGALYCGKLVREGSWLKFQSEHENGRIQIKKS
CFADDLAPVMESCSCYTCLNYSRSYLHHLSKQKANLFTALASIHNVHVLHDVCNHMRHLIINS

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=376, Percent_Identity=35.3723404255319, Blast_Score=225, Evalue=5e-59,
Organism=Homo sapiens, GI13375872, Length=272, Percent_Identity=22.4264705882353, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786607, Length=365, Percent_Identity=41.6438356164384, Blast_Score=274, Evalue=6e-75,
Organism=Caenorhabditis elegans, GI17544680, Length=364, Percent_Identity=36.8131868131868, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI25146502, Length=285, Percent_Identity=24.9122807017544, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19920510, Length=374, Percent_Identity=37.7005347593583, Blast_Score=241, Evalue=6e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42634; Mature: 42634

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
8.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTFSCEVLYQHSGTKARVTRVVTPHGEFITPVFMPVGTRAGVNNMMPSELSAAGTQIIL
CCCEEEEEEEECCCCEEEEEEEECCCCCEECHHEECCCCCCCCCCCCCHHHHHCCCEEEE
GGNTYHMLCAPGMKVIENAGGMHRFMGWHGPMLTDSGGFQVFSLSKNKEICSIDESGAHF
CCCEEEEEECCCHHHHHCCCCHHHHHCCCCCEEECCCCEEEEEECCCCCEEEECCCCCEE
KLPGSKGIIHMTPEMSLETQKIIGADIIMAFDQCTPDSCSKDEVSQIMLRTHRWLKQSID
ECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YHQQYPNSRYGYKQALFGIVQGGVFQDLRRESADFVSAMNTDGIAIGGETVGFDMDTTVE
HHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEECCCEECCCCCHHHH
VIRWVYEYLPGNKPRYTMGVGMSPQDLLDVVAEGIDMFDCVAPTRNARHGALYCGKLVRE
HHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHC
GSWLKFQSEHENGRIQIKKSCFADDLAPVMESCSCYTCLNYSRSYLHHLSKQKANLFTAL
CCCEEEECCCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ASIHNVHVLHDVCNHMRHLIINS
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNTFSCEVLYQHSGTKARVTRVVTPHGEFITPVFMPVGTRAGVNNMMPSELSAAGTQIIL
CCCEEEEEEEECCCCEEEEEEEECCCCCEECHHEECCCCCCCCCCCCCHHHHHCCCEEEE
GGNTYHMLCAPGMKVIENAGGMHRFMGWHGPMLTDSGGFQVFSLSKNKEICSIDESGAHF
CCCEEEEEECCCHHHHHCCCCHHHHHCCCCCEEECCCCEEEEEECCCCCEEEECCCCCEE
KLPGSKGIIHMTPEMSLETQKIIGADIIMAFDQCTPDSCSKDEVSQIMLRTHRWLKQSID
ECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YHQQYPNSRYGYKQALFGIVQGGVFQDLRRESADFVSAMNTDGIAIGGETVGFDMDTTVE
HHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEECCCEECCCCCHHHH
VIRWVYEYLPGNKPRYTMGVGMSPQDLLDVVAEGIDMFDCVAPTRNARHGALYCGKLVRE
HHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHC
GSWLKFQSEHENGRIQIKKSCFADDLAPVMESCSCYTCLNYSRSYLHHLSKQKANLFTAL
CCCEEEECCCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ASIHNVHVLHDVCNHMRHLIINS
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]