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The map label for this gene is greA

Identifier: 54295463

GI number: 54295463

Start: 2910925

End: 2911407

Strand: Reverse

Name: greA

Synonym: lpl2549

Alternate gene names: 54295463

Gene position: 2911407-2910925 (Counterclockwise)

Preceding gene: 54295464

Following gene: 54295461

Centisome position: 87.02

GC content: 40.79

Gene sequence:

>483_bases
ATGAGTAAACATCCTATGACAGTTGAGGGTGCAGAAGCACTAAAAGCAGAATTGCATCGCTTAAAGTTCGTGGATCGTCC
GCGCATTGTAGAAGCAATTGCAACTGCAAGAGCCCATGGTGATTTAAAAGAAAATGCGGAATATCACGCAGCTCGAGAAC
AACAAAGTTTTAATGAAGGGCGCATTCAAGAGCTTGAAGCGAAGTTGTCTCATGCGCAAATAATTGATATCAGTAAATTA
CCTAACAATGGCAAAGTGATATTTGGTTCAACCGTTACAATTTGTCATGTAGCAACGGGCTCAGAATTAACCTATAAAAT
AGTTGGAGAGGATGAAGCTGATATCAAACTGAACAAGATTTCATACAGTTCTCCTATTGCCCGAGCTTTAATTGGCAAAG
AGCTTGATGATGCGGTCACGGTAGAAACACCTGGCGGCATGGTTGAATATGAAATCATCCAGGTGCAATATATTGTTGAG
TGA

Upstream 100 bases:

>100_bases
ACACTACGACCTTATCAGGAGCCGAAGCTGCTTGTTTGGCTATGAAGTATGAAGATAGGGAAACTGTAACTCGCTTACAA
GATTTACATTAGGGGTAAAT

Downstream 100 bases:

>100_bases
TTTCAGTTATTTTCTTTGATTTTTAGTGCAACTGTCATTAAAGACAGTTGCCACTCAGGAAACCATAAGTATTTATGGTT
GAAATACTTCTTGCTGAATA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MSKHPMTVEGAEALKAELHRLKFVDRPRIVEAIATARAHGDLKENAEYHAAREQQSFNEGRIQELEAKLSHAQIIDISKL
PNNGKVIFGSTVTICHVATGSELTYKIVGEDEADIKLNKISYSSPIARALIGKELDDAVTVETPGGMVEYEIIQVQYIVE

Sequences:

>Translated_160_residues
MSKHPMTVEGAEALKAELHRLKFVDRPRIVEAIATARAHGDLKENAEYHAAREQQSFNEGRIQELEAKLSHAQIIDISKL
PNNGKVIFGSTVTICHVATGSELTYKIVGEDEADIKLNKISYSSPIARALIGKELDDAVTVETPGGMVEYEIIQVQYIVE
>Mature_159_residues
SKHPMTVEGAEALKAELHRLKFVDRPRIVEAIATARAHGDLKENAEYHAAREQQSFNEGRIQELEAKLSHAQIIDISKLP
NNGKVIFGSTVTICHVATGSELTYKIVGEDEADIKLNKISYSSPIARALIGKELDDAVTVETPGGMVEYEIIQVQYIVE

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=63.9240506329114, Blast_Score=214, Evalue=3e-57,
Organism=Escherichia coli, GI87082261, Length=157, Percent_Identity=35.031847133758, Blast_Score=85, Evalue=2e-18,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_LEGPA (Q5X1R6)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_124982.1
- HSSP:   P0A6W5
- ProteinModelPortal:   Q5X1R6
- SMR:   Q5X1R6
- STRING:   Q5X1R6
- GeneID:   3117693
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp2677
- LegioList:   lpp2677
- eggNOG:   COG0782
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   LPNE297246:LPP2677-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17669; Mature: 17538

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKHPMTVEGAEALKAELHRLKFVDRPRIVEAIATARAHGDLKENAEYHAAREQQSFNEG
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHCCHHHHHHHHHCCCCC
RIQELEAKLSHAQIIDISKLPNNGKVIFGSTVTICHVATGSELTYKIVGEDEADIKLNKI
HHHHHHHHHCCCEEEEEEECCCCCCEEEECEEEEEEEECCCCEEEEEECCCCCCEEEEEE
SYSSPIARALIGKELDDAVTVETPGGMVEYEIIQVQYIVE
CCCCHHHHHHHCCCCCCCEEEECCCCEEEEEEEEEEEEEC
>Mature Secondary Structure 
SKHPMTVEGAEALKAELHRLKFVDRPRIVEAIATARAHGDLKENAEYHAAREQQSFNEG
CCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHCCHHHHHHHHHCCCCC
RIQELEAKLSHAQIIDISKLPNNGKVIFGSTVTICHVATGSELTYKIVGEDEADIKLNKI
HHHHHHHHHCCCEEEEEEECCCCCCEEEECEEEEEEEECCCCEEEEEECCCCCCEEEEEE
SYSSPIARALIGKELDDAVTVETPGGMVEYEIIQVQYIVE
CCCCHHHHHHHCCCCCCCEEEECCCCEEEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA