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The map label for this gene is def [H]

Identifier: 54295432

GI number: 54295432

Start: 2872646

End: 2873158

Strand: Reverse

Name: def [H]

Synonym: lpl2518

Alternate gene names: 54295432

Gene position: 2873158-2872646 (Counterclockwise)

Preceding gene: 54295437

Following gene: 54295431

Centisome position: 85.88

GC content: 42.69

Gene sequence:

>513_bases
ATGGCAATTCGCAAGATTCTTTATTTACCTGATGAGCGGTTAAGAAAAATAGCTAAGCCGGTCGAGACTTTTGATGAGAG
TTTACAGACATTAATCAACGATATGTTTGATACAATGTATGATGCTCGGGGAGTTGGGCTTGCCGCACCACAAATAGGAG
TCAGCTTGCGTTTATCTGTCATTGATATAGTGGGAGATAAAAAGGAGCAGATCGTTATTGTTAATCCTGAAATAGTTTCC
TCTCATGGCGAAAAAGAGTTTGAAGAAGGCTGTTTGTCTGTCCCAGGGGCTTATGATACCGTTGTCAGAGCTGAAAAAGT
GACCGTGAAGGCACTGGACAGGTTCGGCAAACCGTTTGAAATTACGGGTGAAGGGCTGCTTGCCGAGTGTTTACAACATG
AAATTGACCATATGAATGGCAAATTGTTTGTCGATATGTTGTCTCCATTGAAGCGAATGATGGCTCGCCGGAAGTTAGAT
AAGTTCAAGCGTTTACAAGCTCGTAAGCCATGA

Upstream 100 bases:

>100_bases
ATCTCATGTATCAATCCCTTTAGTTGAGCCTTAGCATCTTGCAGCATTACTGTATATCGGGGATAATATCACGATTAACG
TGTGGAGAACTAGATAAACA

Downstream 100 bases:

>100_bases
ATGGTTTAACTGTCGTTTTTGCTGGAACTCCAGAATTTGGCTTACCCTGTCTGGATGCTTTAATTCAATCAAGGCATCAT
CTCAAGGCTGTTTATACTCA

Product: hypothetical protein

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MAIRKILYLPDERLRKIAKPVETFDESLQTLINDMFDTMYDARGVGLAAPQIGVSLRLSVIDIVGDKKEQIVIVNPEIVS
SHGEKEFEEGCLSVPGAYDTVVRAEKVTVKALDRFGKPFEITGEGLLAECLQHEIDHMNGKLFVDMLSPLKRMMARRKLD
KFKRLQARKP

Sequences:

>Translated_170_residues
MAIRKILYLPDERLRKIAKPVETFDESLQTLINDMFDTMYDARGVGLAAPQIGVSLRLSVIDIVGDKKEQIVIVNPEIVS
SHGEKEFEEGCLSVPGAYDTVVRAEKVTVKALDRFGKPFEITGEGLLAECLQHEIDHMNGKLFVDMLSPLKRMMARRKLD
KFKRLQARKP
>Mature_169_residues
AIRKILYLPDERLRKIAKPVETFDESLQTLINDMFDTMYDARGVGLAAPQIGVSLRLSVIDIVGDKKEQIVIVNPEIVSS
HGEKEFEEGCLSVPGAYDTVVRAEKVTVKALDRFGKPFEITGEGLLAECLQHEIDHMNGKLFVDMLSPLKRMMARRKLDK
FKRLQARKP

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=168, Percent_Identity=51.7857142857143, Blast_Score=192, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24645728, Length=165, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24645726, Length=137, Percent_Identity=30.6569343065693, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19228; Mature: 19097

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRKILYLPDERLRKIAKPVETFDESLQTLINDMFDTMYDARGVGLAAPQIGVSLRLSV
CCCCHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
IDIVGDKKEQIVIVNPEIVSSHGEKEFEEGCLSVPGAYDTVVRAEKVTVKALDRFGKPFE
HEEECCCCCEEEEECCHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
ITGEGLLAECLQHEIDHMNGKLFVDMLSPLKRMMARRKLDKFKRLQARKP
ECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AIRKILYLPDERLRKIAKPVETFDESLQTLINDMFDTMYDARGVGLAAPQIGVSLRLSV
CCCHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
IDIVGDKKEQIVIVNPEIVSSHGEKEFEEGCLSVPGAYDTVVRAEKVTVKALDRFGKPFE
HEEECCCCCEEEEECCHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
ITGEGLLAECLQHEIDHMNGKLFVDMLSPLKRMMARRKLDKFKRLQARKP
ECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]