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The map label for this gene is rsmB [H]

Identifier: 54295430

GI number: 54295430

Start: 2870425

End: 2871708

Strand: Reverse

Name: rsmB [H]

Synonym: lpl2516

Alternate gene names: 54295430

Gene position: 2871708-2870425 (Counterclockwise)

Preceding gene: 54295431

Following gene: 54295429

Centisome position: 85.83

GC content: 42.91

Gene sequence:

>1284_bases
ATGAAAGTAAATGACAGATTCAACGCCTTAAAAATTTTGACGGCCTTGATAGAAAGCAGAAAAAACCTGTCCTATTTGAT
GTCTCCGCAAGAGATTTCTCCCATGACAAAAGAAATTTGCTATGGATTTTGTCGTCATTATTTTCGTTTACAATCCATAG
CTGATACTTTAATCAATAAGCGACCTAAAGATAATGAGGTATGGATAGTCTTGTTGATAGGTATTTATCAATTGCAGTAT
ATGAATAAGCCGGATTATGCCGTGGTGAAGGAGACAGTGTCTTTACTGGGGCAGGTTAAAAAAAGTTGGGCAAAAGGACT
GGTAAATGCCGTGCTAAGGAATTTTTGCCGTCGACAAGACCATATTCTGGAAAAGTTGGCAGCAGAACCGGTTTTTATCT
ATGGACAGCCGAATTGGTTACTTCAACGCCTGCAAAAAAACTGGCCAAACGATTGGCAATTCATCGCTGAAGCGAATGAT
AGGCACCCACCCATGACTTTGAGAGTCAATGTCTTGAAACACTCAGTTGATGAGTATCTTGATTTACTAAAAAAATCCGG
GATAGAGGCTTTTAAACACCCTGTCGCACCTGATGGAATTACTTTGGCAATCCCTTGTGATGTGATGACGCTTCCTGGTT
TTGCGCAAGGTTCTGTGTCTGTTCAGGATGCCGCGCCTCAATTAGCAGCGTATTTATTGTCTTTAAAACCCGGGCAGCGA
GTACTGGATGCCTGTTGCGCTCCTGGCGGAAAAACATGTCATATTTTGGAGAAAGAGTCTGGTTTGGCTTCCTGTGTTGC
ATTGGATTTAGACCCACAGCGCTTACAGCGGGTCAGAGATAACTTAAATCGATTGGATTTGCAAGCAACGGTGGTGGAAG
GTGATGTATTGGAACCCAGAAGCTGGTGGGATGGTCATCCATTTGATCGTATTTTACTGGATGCGCCCTGTTCCGCTACA
GGAGTAATTAGACGCCACTCTGATATCAAATTATTACGAACGGATGAAGAGATTTCAGCAATTTCTCAGGTACAATCCAG
CATGTTGAATTCATTATGGCCACTGCTTGCACCAGGTGGTTTAATGGTTTATGCCACATGCTCTATTATGAGTGATGAAA
ATGAGTTGCAAATCGCCCGATTTGTTGCAGAGCATTCGGATTGTCAAGTGAGTGATGGTAAATGGTCCTGGGGAAGGTGG
ACTGGGCATGGTCAACAGATATTGCCTGGGGAGCATGGCATGGATGGCTTTTTTTACAGTGTATTGCGAAAGGATGAAAA
ATGA

Upstream 100 bases:

>100_bases
TGTTAGTGGAAAAGATTCAATTTCCCGGAGCAAAGGTCATTTCAGTCAGGGATTGGTTAAATTCGGGTAAAACACAATTG
CATACTGGTTTAATGTTGCA

Downstream 100 bases:

>100_bases
TAATTGATTGGCCTGTAATTATTGTCTTGTTTTGTTTATCCATTCCTGGTGTTTTAATCGTGATGCATCGCCTGGTATAT
TTTCTGCTAGCCGATAATAC

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKVNDRFNALKILTALIESRKNLSYLMSPQEISPMTKEICYGFCRHYFRLQSIADTLINKRPKDNEVWIVLLIGIYQLQY
MNKPDYAVVKETVSLLGQVKKSWAKGLVNAVLRNFCRRQDHILEKLAAEPVFIYGQPNWLLQRLQKNWPNDWQFIAEAND
RHPPMTLRVNVLKHSVDEYLDLLKKSGIEAFKHPVAPDGITLAIPCDVMTLPGFAQGSVSVQDAAPQLAAYLLSLKPGQR
VLDACCAPGGKTCHILEKESGLASCVALDLDPQRLQRVRDNLNRLDLQATVVEGDVLEPRSWWDGHPFDRILLDAPCSAT
GVIRRHSDIKLLRTDEEISAISQVQSSMLNSLWPLLAPGGLMVYATCSIMSDENELQIARFVAEHSDCQVSDGKWSWGRW
TGHGQQILPGEHGMDGFFYSVLRKDEK

Sequences:

>Translated_427_residues
MKVNDRFNALKILTALIESRKNLSYLMSPQEISPMTKEICYGFCRHYFRLQSIADTLINKRPKDNEVWIVLLIGIYQLQY
MNKPDYAVVKETVSLLGQVKKSWAKGLVNAVLRNFCRRQDHILEKLAAEPVFIYGQPNWLLQRLQKNWPNDWQFIAEAND
RHPPMTLRVNVLKHSVDEYLDLLKKSGIEAFKHPVAPDGITLAIPCDVMTLPGFAQGSVSVQDAAPQLAAYLLSLKPGQR
VLDACCAPGGKTCHILEKESGLASCVALDLDPQRLQRVRDNLNRLDLQATVVEGDVLEPRSWWDGHPFDRILLDAPCSAT
GVIRRHSDIKLLRTDEEISAISQVQSSMLNSLWPLLAPGGLMVYATCSIMSDENELQIARFVAEHSDCQVSDGKWSWGRW
TGHGQQILPGEHGMDGFFYSVLRKDEK
>Mature_427_residues
MKVNDRFNALKILTALIESRKNLSYLMSPQEISPMTKEICYGFCRHYFRLQSIADTLINKRPKDNEVWIVLLIGIYQLQY
MNKPDYAVVKETVSLLGQVKKSWAKGLVNAVLRNFCRRQDHILEKLAAEPVFIYGQPNWLLQRLQKNWPNDWQFIAEAND
RHPPMTLRVNVLKHSVDEYLDLLKKSGIEAFKHPVAPDGITLAIPCDVMTLPGFAQGSVSVQDAAPQLAAYLLSLKPGQR
VLDACCAPGGKTCHILEKESGLASCVALDLDPQRLQRVRDNLNRLDLQATVVEGDVLEPRSWWDGHPFDRILLDAPCSAT
GVIRRHSDIKLLRTDEEISAISQVQSSMLNSLWPLLAPGGLMVYATCSIMSDENELQIARFVAEHSDCQVSDGKWSWGRW
TGHGQQILPGEHGMDGFFYSVLRKDEK

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=353, Percent_Identity=28.0453257790368, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI76150623, Length=353, Percent_Identity=28.0453257790368, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI32698918, Length=174, Percent_Identity=31.6091954022989, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI8922322, Length=305, Percent_Identity=28.1967213114754, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI270288818, Length=305, Percent_Identity=28.1967213114754, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI23199998, Length=305, Percent_Identity=28.1967213114754, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI270288816, Length=305, Percent_Identity=28.1967213114754, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI11545785, Length=183, Percent_Identity=32.2404371584699, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI40316918, Length=187, Percent_Identity=31.5508021390374, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI39995082, Length=181, Percent_Identity=30.939226519337, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI301336155, Length=181, Percent_Identity=30.939226519337, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI2367212, Length=429, Percent_Identity=44.2890442890443, Blast_Score=336, Evalue=2e-93,
Organism=Escherichia coli, GI87081985, Length=249, Percent_Identity=28.9156626506024, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17536757, Length=345, Percent_Identity=31.0144927536232, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI25143471, Length=177, Percent_Identity=31.0734463276836, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324268, Length=343, Percent_Identity=27.1137026239067, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319447, Length=259, Percent_Identity=30.1158301158301, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI22024126, Length=316, Percent_Identity=25.9493670886076, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24668781, Length=222, Percent_Identity=31.0810810810811, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356513, Length=222, Percent_Identity=31.0810810810811, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19920494, Length=173, Percent_Identity=28.9017341040462, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21355201, Length=185, Percent_Identity=27.027027027027, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 48248; Mature: 48248

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVNDRFNALKILTALIESRKNLSYLMSPQEISPMTKEICYGFCRHYFRLQSIADTLINK
CCCCCHHHHHHHHHHHHHCCCCHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
RPKDNEVWIVLLIGIYQLQYMNKPDYAVVKETVSLLGQVKKSWAKGLVNAVLRNFCRRQD
CCCCCCEEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HILEKLAAEPVFIYGQPNWLLQRLQKNWPNDWQFIAEANDRHPPMTLRVNVLKHSVDEYL
HHHHHHCCCCEEEEECCHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHH
DLLKKSGIEAFKHPVAPDGITLAIPCDVMTLPGFAQGSVSVQDAAPQLAAYLLSLKPGQR
HHHHHCCHHHHHCCCCCCCEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHH
VLDACCAPGGKTCHILEKESGLASCVALDLDPQRLQRVRDNLNRLDLQATVVEGDVLEPR
HHHHHCCCCCCEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEEECCCCCCCC
SWWDGHPFDRILLDAPCSATGVIRRHSDIKLLRTDEEISAISQVQSSMLNSLWPLLAPGG
CCCCCCCCCCEEEECCCCCHHHHEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCC
LMVYATCSIMSDENELQIARFVAEHSDCQVSDGKWSWGRWTGHGQQILPGEHGMDGFFYS
EEEEEEEEHCCCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCEECCCCCCCHHHHHH
VLRKDEK
HHHCCCC
>Mature Secondary Structure
MKVNDRFNALKILTALIESRKNLSYLMSPQEISPMTKEICYGFCRHYFRLQSIADTLINK
CCCCCHHHHHHHHHHHHHCCCCHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
RPKDNEVWIVLLIGIYQLQYMNKPDYAVVKETVSLLGQVKKSWAKGLVNAVLRNFCRRQD
CCCCCCEEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HILEKLAAEPVFIYGQPNWLLQRLQKNWPNDWQFIAEANDRHPPMTLRVNVLKHSVDEYL
HHHHHHCCCCEEEEECCHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHH
DLLKKSGIEAFKHPVAPDGITLAIPCDVMTLPGFAQGSVSVQDAAPQLAAYLLSLKPGQR
HHHHHCCHHHHHCCCCCCCEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHH
VLDACCAPGGKTCHILEKESGLASCVALDLDPQRLQRVRDNLNRLDLQATVVEGDVLEPR
HHHHHCCCCCCEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEEECCCCCCCC
SWWDGHPFDRILLDAPCSATGVIRRHSDIKLLRTDEEISAISQVQSSMLNSLWPLLAPGG
CCCCCCCCCCEEEECCCCCHHHHEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCC
LMVYATCSIMSDENELQIARFVAEHSDCQVSDGKWSWGRWTGHGQQILPGEHGMDGFFYS
EEEEEEEEHCCCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCEECCCCCCCHHHHHH
VLRKDEK
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]