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The map label for this gene is sflR [H]

Identifier: 54295274

GI number: 54295274

Start: 2689900

End: 2691015

Strand: Reverse

Name: sflR [H]

Synonym: lpl2359

Alternate gene names: 54295274

Gene position: 2691015-2689900 (Counterclockwise)

Preceding gene: 54295275

Following gene: 54295273

Centisome position: 80.43

GC content: 45.16

Gene sequence:

>1116_bases
ATGGATGTTTTTGTGCCCGCAATTCCGGAAATGAGTCGTTATTTCAATACTGACAGTACAACGATGCAGGCTACCCTTTA
TTTATTTATGCTTACTGTAGCTTTGGGTCAGCTATTGGTTGGGCCTTTAGCCGATCGTTTTGGACGAAGACAGATGGCTT
TGAGTACGGCACTTCTTTTTTTTGCAGGATCGTTGATCTCTGCGTTAGCTCCGTCAGTGCCGGCATTGATTTTTGCACGT
ATCATTCAGGCAGCTGGTGCCTGTGGAACCTATTTACTATGCTTTATTATCGTTCGAGATAATTTTTCAACGGGCACCTG
TGCCCGCTTGTTTAGCATCCTGTCTGGGACGAATTCGATGGTTGCCAGTTCTGCTCCAGTCATTGGGGGATTATTGTTGG
ATATGACGCAGGATTGGCATAGTGGATTCTACTTTTTAACCTTGCTTGGGTTATTAATGAGTGGCATAGCCTTCCGTTAC
ATCCCCGATTATCACTATCCCAAACCCGAGCTGCCCCAGATGAGGGTGCACCAAGTGATGAGGCAGATAATAAGTAACTC
GGACTTTCGCCAATACGCATGGGTTGCATCAGCCAGTTTGCTGGGATTGTACTTATTTTGTGCTCAATCACCAGGTATTT
TGATCACACAACTGCACCTGAGTGCGACTGAATATGGTTTCTGGTTTGGTTTGAATGCAATGACTGTGTTTGTTGCCAAT
ATGATGGCCGCACGACTTACTTATTCTCACTCATTGGAGAGCATAGTGCGTTGGGGCTTGGTTTTAATGACACTGTCATG
CTTTTTTATGCTGGCTCTTAATCTTCGTCAATGCAGCATTTTAAGTTTTATGCTTCCCATGCTTTGCTTGACTGTGGGTA
TTGGTATTAGCATGGGCACAGCAACAGCACTTGCTTTAAAGGATTTTGAACAGCAAGCTGGTGTAGCTACCGCTCTGTTA
GGTGCTTGCCAGTTTGGTCTCGCGGGCCTGATTGGTGTTTTGACAGCTCAATGGATGCCCGGGCCATTAAGTGTAGCTAT
CCCCGTACTTTGTTTCACTGCAGTGGGATTGGCTAGAATCACCAAATTCAGTTTTAGAATATTGAGCGGCTCTTAA

Upstream 100 bases:

>100_bases
AATATTTTAACAGGAACACCATTAATTGAATTTTATTGGCTGGACAGAAAGACAAAGTCTTTTTCGATTATTTCCACTCA
TTATCAGCATTTCTTTTGCT

Downstream 100 bases:

>100_bases
TCTTGTCCGCAGGCAATTGGCCAAAAAAGACCGATTACGGGCTATGTTGTAGGTAACACTGTGGCATTTAATGTTGTTTA
TCCCGGGTGTGGTTCTGTAT

Product: hypothetical protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MDVFVPAIPEMSRYFNTDSTTMQATLYLFMLTVALGQLLVGPLADRFGRRQMALSTALLFFAGSLISALAPSVPALIFAR
IIQAAGACGTYLLCFIIVRDNFSTGTCARLFSILSGTNSMVASSAPVIGGLLLDMTQDWHSGFYFLTLLGLLMSGIAFRY
IPDYHYPKPELPQMRVHQVMRQIISNSDFRQYAWVASASLLGLYLFCAQSPGILITQLHLSATEYGFWFGLNAMTVFVAN
MMAARLTYSHSLESIVRWGLVLMTLSCFFMLALNLRQCSILSFMLPMLCLTVGIGISMGTATALALKDFEQQAGVATALL
GACQFGLAGLIGVLTAQWMPGPLSVAIPVLCFTAVGLARITKFSFRILSGS

Sequences:

>Translated_371_residues
MDVFVPAIPEMSRYFNTDSTTMQATLYLFMLTVALGQLLVGPLADRFGRRQMALSTALLFFAGSLISALAPSVPALIFAR
IIQAAGACGTYLLCFIIVRDNFSTGTCARLFSILSGTNSMVASSAPVIGGLLLDMTQDWHSGFYFLTLLGLLMSGIAFRY
IPDYHYPKPELPQMRVHQVMRQIISNSDFRQYAWVASASLLGLYLFCAQSPGILITQLHLSATEYGFWFGLNAMTVFVAN
MMAARLTYSHSLESIVRWGLVLMTLSCFFMLALNLRQCSILSFMLPMLCLTVGIGISMGTATALALKDFEQQAGVATALL
GACQFGLAGLIGVLTAQWMPGPLSVAIPVLCFTAVGLARITKFSFRILSGS
>Mature_371_residues
MDVFVPAIPEMSRYFNTDSTTMQATLYLFMLTVALGQLLVGPLADRFGRRQMALSTALLFFAGSLISALAPSVPALIFAR
IIQAAGACGTYLLCFIIVRDNFSTGTCARLFSILSGTNSMVASSAPVIGGLLLDMTQDWHSGFYFLTLLGLLMSGIAFRY
IPDYHYPKPELPQMRVHQVMRQIISNSDFRQYAWVASASLLGLYLFCAQSPGILITQLHLSATEYGFWFGLNAMTVFVAN
MMAARLTYSHSLESIVRWGLVLMTLSCFFMLALNLRQCSILSFMLPMLCLTVGIGISMGTATALALKDFEQQAGVATALL
GACQFGLAGLIGVLTAQWMPGPLSVAIPVLCFTAVGLARITKFSFRILSGS

Specific function: Appears to provoke a reduction of the content of the major porins ompA and ompC [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=341, Percent_Identity=26.9794721407625, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI87082312, Length=339, Percent_Identity=25.6637168141593, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1790146, Length=330, Percent_Identity=23.3333333333333, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI48994889, Length=363, Percent_Identity=23.1404958677686, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 40306; Mature: 40306

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVFVPAIPEMSRYFNTDSTTMQATLYLFMLTVALGQLLVGPLADRFGRRQMALSTALLF
CCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
FAGSLISALAPSVPALIFARIIQAAGACGTYLLCFIIVRDNFSTGTCARLFSILSGTNSM
HHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHH
VASSAPVIGGLLLDMTQDWHSGFYFLTLLGLLMSGIAFRYIPDYHYPKPELPQMRVHQVM
HHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
RQIISNSDFRQYAWVASASLLGLYLFCAQSPGILITQLHLSATEYGFWFGLNAMTVFVAN
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHH
MMAARLTYSHSLESIVRWGLVLMTLSCFFMLALNLRQCSILSFMLPMLCLTVGIGISMGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ATALALKDFEQQAGVATALLGACQFGLAGLIGVLTAQWMPGPLSVAIPVLCFTAVGLARI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
TKFSFRILSGS
HHHHHEEECCC
>Mature Secondary Structure
MDVFVPAIPEMSRYFNTDSTTMQATLYLFMLTVALGQLLVGPLADRFGRRQMALSTALLF
CCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
FAGSLISALAPSVPALIFARIIQAAGACGTYLLCFIIVRDNFSTGTCARLFSILSGTNSM
HHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHH
VASSAPVIGGLLLDMTQDWHSGFYFLTLLGLLMSGIAFRYIPDYHYPKPELPQMRVHQVM
HHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
RQIISNSDFRQYAWVASASLLGLYLFCAQSPGILITQLHLSATEYGFWFGLNAMTVFVAN
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHH
MMAARLTYSHSLESIVRWGLVLMTLSCFFMLALNLRQCSILSFMLPMLCLTVGIGISMGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ATALALKDFEQQAGVATALLGACQFGLAGLIGVLTAQWMPGPLSVAIPVLCFTAVGLARI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
TKFSFRILSGS
HHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1648560; 1658833 [H]