Click here to switch to the map view.

The map label for this gene is miaB

Identifier: 54294222

GI number: 54294222

Start: 1437630

End: 1438973

Strand: Reverse

Name: miaB

Synonym: lpl1287

Alternate gene names: 54294222

Gene position: 1438973-1437630 (Counterclockwise)

Preceding gene: 54294223

Following gene: 54294221

Centisome position: 43.01

GC content: 39.58

Gene sequence:

>1344_bases
ATGGTTAAAAAATTATATATTAAAACAAATGGCTGCCAAATGAATGAATACGATTCTTCTAAAATGGCAGAAGTTCTTTA
CGCTTCCCATGGTTTGGTCAAAACTGATCAAGTTGAGGATGCCGATGTTATTTTATTAAACACATGCTCAATTCGAGAAA
AAGCACAGGAAAAAGTTTTTTCTCAACTGGGTCAATGGCGGGAATATAAAGCCAAAAACCCTCATGTGCTCATTGGAGTG
GGAGGGTGCGTAGCGAGCCAGGAAGGCTCAGACATCATAAAAAGAGCACCGTTTGTTGATATCGTATTTGGGCCACAAAC
ATTGCACAGGCTCCCTGCCCTGCTCAATGAAAGGCTTGAAAAAAATAAATCCGTTGTTGATATCAGCTTTCCAGAAATAG
AGAAATTTGATCATTTACCCGCACCAAGAGCAGAAGGCCCTACAGCTTTTGTATCTATAATGGAAGGCTGTAGTAAATAT
TGCAGCTTCTGTGTAGTGCCCTACACGCGTGGCGAAGAAATCAGCAGACCATTTGATGATGTATTGGCTGAGTGTTATCA
ATTAGCCAGTCAAGGCGTCAGAGAGATTAATTTACTAGGCCAAAATGTGAACGATTACAGAGGTATTATGGATAATGGGG
ATATAGCCGATCTTGCCCTGTTAATTCATTATATTGCCGCTATTGATGGAATAGGAAGAATACGTTTCACAACATCACAT
CCCTTGGCATTCTCTGAAAATTTGATTAATGCCTACGCTGAGGTTCCAGAGCTTGCTAATCATTTACACCTTCCGGTACA
AAGCGGATCCGATCGCATTTTATCGTTAATGAAACGAGGGTACACTGCTTTGGAATTTAAATCCAAAATTCGTAAATTAA
GGAAAATTCGACCAGACATTCGCTTATCTACTGACATTATTGTAGGCTTTCCTGGAGAGACAGACAAAGATTTCCAGGAC
ACTATGGATCTTGTACATGAAATCGGTTTTGATACCTCTTTCAGTTTTATATACAGCCCAAGACCAGGAACTCCTGCAGC
AAATTTACCTGATGACACACCTATGGAGATCAAAAAGCAGCGTTTACAGATTTTACAAAACAGATTATTAATGAATGCAG
CTCGTTACAGTGAATCAATGATAGGCAGCAAGCAAAAAATTCTGGTTACTGGATTTTCCAAGAAAAGTTCCCAGCAATTA
TCTGGTCGTACTGAATGTAACCGTGTAGTTAATTTTGATGGACCACCTCATTTAATTGGACAATTTATAGATGTTCAAAT
TAGTGATGCACTACCTAATTCATTGCGAGGAAGGCTGCTTGAAAAGGAGATGCAGCCAGCCTAA

Upstream 100 bases:

>100_bases
CATGTTAGTTGCGTTACTAATATTACCGAAAATCAAAGAAACCTATTGCCAAAGTGTCAATTAATGAAATAATACCGCAA
TTTCAATTTGTAGAATCAAA

Downstream 100 bases:

>100_bases
TGATTGAGCATATCCAGCAACAATTATCAGTACCTCGCGAATTACATAACCAACGAGTAGATAGCGTGCTTGCCAGGCTG
CTTCCTGAGTATTCACGCTC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MVKKLYIKTNGCQMNEYDSSKMAEVLYASHGLVKTDQVEDADVILLNTCSIREKAQEKVFSQLGQWREYKAKNPHVLIGV
GGCVASQEGSDIIKRAPFVDIVFGPQTLHRLPALLNERLEKNKSVVDISFPEIEKFDHLPAPRAEGPTAFVSIMEGCSKY
CSFCVVPYTRGEEISRPFDDVLAECYQLASQGVREINLLGQNVNDYRGIMDNGDIADLALLIHYIAAIDGIGRIRFTTSH
PLAFSENLINAYAEVPELANHLHLPVQSGSDRILSLMKRGYTALEFKSKIRKLRKIRPDIRLSTDIIVGFPGETDKDFQD
TMDLVHEIGFDTSFSFIYSPRPGTPAANLPDDTPMEIKKQRLQILQNRLLMNAARYSESMIGSKQKILVTGFSKKSSQQL
SGRTECNRVVNFDGPPHLIGQFIDVQISDALPNSLRGRLLEKEMQPA

Sequences:

>Translated_447_residues
MVKKLYIKTNGCQMNEYDSSKMAEVLYASHGLVKTDQVEDADVILLNTCSIREKAQEKVFSQLGQWREYKAKNPHVLIGV
GGCVASQEGSDIIKRAPFVDIVFGPQTLHRLPALLNERLEKNKSVVDISFPEIEKFDHLPAPRAEGPTAFVSIMEGCSKY
CSFCVVPYTRGEEISRPFDDVLAECYQLASQGVREINLLGQNVNDYRGIMDNGDIADLALLIHYIAAIDGIGRIRFTTSH
PLAFSENLINAYAEVPELANHLHLPVQSGSDRILSLMKRGYTALEFKSKIRKLRKIRPDIRLSTDIIVGFPGETDKDFQD
TMDLVHEIGFDTSFSFIYSPRPGTPAANLPDDTPMEIKKQRLQILQNRLLMNAARYSESMIGSKQKILVTGFSKKSSQQL
SGRTECNRVVNFDGPPHLIGQFIDVQISDALPNSLRGRLLEKEMQPA
>Mature_447_residues
MVKKLYIKTNGCQMNEYDSSKMAEVLYASHGLVKTDQVEDADVILLNTCSIREKAQEKVFSQLGQWREYKAKNPHVLIGV
GGCVASQEGSDIIKRAPFVDIVFGPQTLHRLPALLNERLEKNKSVVDISFPEIEKFDHLPAPRAEGPTAFVSIMEGCSKY
CSFCVVPYTRGEEISRPFDDVLAECYQLASQGVREINLLGQNVNDYRGIMDNGDIADLALLIHYIAAIDGIGRIRFTTSH
PLAFSENLINAYAEVPELANHLHLPVQSGSDRILSLMKRGYTALEFKSKIRKLRKIRPDIRLSTDIIVGFPGETDKDFQD
TMDLVHEIGFDTSFSFIYSPRPGTPAANLPDDTPMEIKKQRLQILQNRLLMNAARYSESMIGSKQKILVTGFSKKSSQQL
SGRTECNRVVNFDGPPHLIGQFIDVQISDALPNSLRGRLLEKEMQPA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=481, Percent_Identity=34.9272349272349, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI28872782, Length=481, Percent_Identity=34.9272349272349, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI93277076, Length=455, Percent_Identity=30.989010989011, Blast_Score=179, Evalue=4e-45,
Organism=Escherichia coli, GI1786882, Length=443, Percent_Identity=65.0112866817156, Blast_Score=608, Evalue=1e-175,
Organism=Escherichia coli, GI1787057, Length=379, Percent_Identity=29.023746701847, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=470, Percent_Identity=33.4042553191489, Blast_Score=249, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI71996771, Length=370, Percent_Identity=27.027027027027, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21356207, Length=479, Percent_Identity=32.776617954071, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI19922432, Length=462, Percent_Identity=29.004329004329, Blast_Score=152, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_LEGPA (Q5X5N2)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_123612.1
- ProteinModelPortal:   Q5X5N2
- SMR:   Q5X5N2
- STRING:   Q5X5N2
- GeneID:   3118967
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp1288
- LegioList:   lpp1288
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- ProtClustDB:   PRK14325
- BioCyc:   LPNE297246:LPP1288-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50051; Mature: 50051

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKLYIKTNGCQMNEYDSSKMAEVLYASHGLVKTDQVEDADVILLNTCSIREKAQEKVF
CCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCEEEEECCHHHHHHHHHHH
SQLGQWREYKAKNPHVLIGVGGCVASQEGSDIIKRAPFVDIVFGPQTLHRLPALLNERLE
HHHHHHHHHCCCCCEEEEECCCEECCCCCCHHHHHCCEEEEEECHHHHHHHHHHHHHHHH
KNKSVVDISFPEIEKFDHLPAPRAEGPTAFVSIMEGCSKYCSFCVVPYTRGEEISRPFDD
CCCCEEEEECCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHH
VLAECYQLASQGVREINLLGQNVNDYRGIMDNGDIADLALLIHYIAAIDGIGRIRFTTSH
HHHHHHHHHHCCHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCC
PLAFSENLINAYAEVPELANHLHLPVQSGSDRILSLMKRGYTALEFKSKIRKLRKIRPDI
CCHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
RLSTDIIVGFPGETDKDFQDTMDLVHEIGFDTSFSFIYSPRPGTPAANLPDDTPMEIKKQ
EEECEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHH
RLQILQNRLLMNAARYSESMIGSKQKILVTGFSKKSSQQLSGRTECNRVVNFDGPPHLIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCHHHCEEECCCCCHHHHH
QFIDVQISDALPNSLRGRLLEKEMQPA
HHHEEEEHHCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVKKLYIKTNGCQMNEYDSSKMAEVLYASHGLVKTDQVEDADVILLNTCSIREKAQEKVF
CCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCEEEEECCHHHHHHHHHHH
SQLGQWREYKAKNPHVLIGVGGCVASQEGSDIIKRAPFVDIVFGPQTLHRLPALLNERLE
HHHHHHHHHCCCCCEEEEECCCEECCCCCCHHHHHCCEEEEEECHHHHHHHHHHHHHHHH
KNKSVVDISFPEIEKFDHLPAPRAEGPTAFVSIMEGCSKYCSFCVVPYTRGEEISRPFDD
CCCCEEEEECCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHH
VLAECYQLASQGVREINLLGQNVNDYRGIMDNGDIADLALLIHYIAAIDGIGRIRFTTSH
HHHHHHHHHHCCHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCC
PLAFSENLINAYAEVPELANHLHLPVQSGSDRILSLMKRGYTALEFKSKIRKLRKIRPDI
CCHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
RLSTDIIVGFPGETDKDFQDTMDLVHEIGFDTSFSFIYSPRPGTPAANLPDDTPMEIKKQ
EEECEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHH
RLQILQNRLLMNAARYSESMIGSKQKILVTGFSKKSSQQLSGRTECNRVVNFDGPPHLIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCHHHCEEECCCCCHHHHH
QFIDVQISDALPNSLRGRLLEKEMQPA
HHHEEEEHHCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA