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The map label for this gene is pyrE

Identifier: 54294148

GI number: 54294148

Start: 1355006

End: 1355641

Strand: Reverse

Name: pyrE

Synonym: lpl1212

Alternate gene names: 54294148

Gene position: 1355641-1355006 (Counterclockwise)

Preceding gene: 54294152

Following gene: 54294145

Centisome position: 40.52

GC content: 38.05

Gene sequence:

>636_bases
ATGAATCATACCAAATCATCTTTTATAAAATTAGCTCTGGAGTGTCAAGTACTTAAATTTGGTGAATTTACTTTAAAATC
AGGACGAATAAGCCCCTATTTCTTTAATGCTGGTTTATTTTATCATGGTAATTCCATACGTCAGTTAGGTCAATTTTATG
CAAAAACACTTTTAGAACAAGAAATTTCTTTTGAGCACTTGTTTGGACCAGCGTACAAAGGTATCCCACTCGCTACTGCA
ACTGCAGTGGCCTTAGCTGAGTTAGGCAGGGACATCACAGTGACTTTTAATCGTAAAGAAGTAAAAACTCATGGAGAAGG
CGGACAATTAATTGGTTCACCGCTCACAGGAAGGACAGTGATCATTGACGATGTGATTACAGCGGGAACAGCATTCAGAG
AATCACAAACCCTGATTAAAGAGAATGGAGGAATTCTTCGTGGTGTCATTATCGCCCTGGACAGATGCGAACGAGGATTA
ACTGAGAAATCAACCCTGTCAGAAATCAGGGAGCAAGGAATTGAAGTCTATTCAATTATCAATTTGTTTGATCTAATAGA
ATTTCTGAAAAATGACAATCAATACGAACAGGTACAAAAACTGGAGTCTTATCACGAGCTTTATGGCGCATATTAG

Upstream 100 bases:

>100_bases
ATCCCGCACTATGATTAGCATATCAGTTTTTTGTTGATTGTTAAGGATTTTTTTAAAATATGTGATTGTTTTCAATAAAA
TTTTGTATTAGGAAACCAGA

Downstream 100 bases:

>100_bases
GATCAGGACTTACGAAAAACCCCAATCTCTTTGTTAAAAAATGTTTTTAATTCGGTCATTTAGTGTATCTAAACTCTCTC
ATTAAAAACATTTTTTCCTC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MNHTKSSFIKLALECQVLKFGEFTLKSGRISPYFFNAGLFYHGNSIRQLGQFYAKTLLEQEISFEHLFGPAYKGIPLATA
TAVALAELGRDITVTFNRKEVKTHGEGGQLIGSPLTGRTVIIDDVITAGTAFRESQTLIKENGGILRGVIIALDRCERGL
TEKSTLSEIREQGIEVYSIINLFDLIEFLKNDNQYEQVQKLESYHELYGAY

Sequences:

>Translated_211_residues
MNHTKSSFIKLALECQVLKFGEFTLKSGRISPYFFNAGLFYHGNSIRQLGQFYAKTLLEQEISFEHLFGPAYKGIPLATA
TAVALAELGRDITVTFNRKEVKTHGEGGQLIGSPLTGRTVIIDDVITAGTAFRESQTLIKENGGILRGVIIALDRCERGL
TEKSTLSEIREQGIEVYSIINLFDLIEFLKNDNQYEQVQKLESYHELYGAY
>Mature_211_residues
MNHTKSSFIKLALECQVLKFGEFTLKSGRISPYFFNAGLFYHGNSIRQLGQFYAKTLLEQEISFEHLFGPAYKGIPLATA
TAVALAELGRDITVTFNRKEVKTHGEGGQLIGSPLTGRTVIIDDVITAGTAFRESQTLIKENGGILRGVIIALDRCERGL
TEKSTLSEIREQGIEVYSIINLFDLIEFLKNDNQYEQVQKLESYHELYGAY

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=185, Percent_Identity=28.6486486486486, Blast_Score=74, Evalue=9e-14,
Organism=Escherichia coli, GI1790073, Length=212, Percent_Identity=55.188679245283, Blast_Score=218, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17508631, Length=192, Percent_Identity=25, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323530, Length=218, Percent_Identity=46.3302752293578, Blast_Score=180, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6323927, Length=215, Percent_Identity=43.2558139534884, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_LEGPL (Q5WX86)

Other databases:

- EMBL:   CR628337
- RefSeq:   YP_126563.1
- HSSP:   P08870
- ProteinModelPortal:   Q5WX86
- SMR:   Q5WX86
- STRING:   Q5WX86
- GeneID:   3113333
- GenomeReviews:   CR628337_GR
- KEGG:   lpf:lpl1212
- NMPDR:   fig|297245.3.peg.2371
- LegioList:   lpl1212
- eggNOG:   COG0461
- HOGENOM:   HBG404341
- OMA:   FAFNRKE
- PhylomeDB:   Q5WX86
- ProtClustDB:   PRK00455
- BioCyc:   LPNE297245:LPL1212-MONOMER
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 23634; Mature: 23634

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 26-26 BINDING 98-98 BINDING 99-99 BINDING 102-102 BINDING 104-104 BINDING 127-127 BINDING 155-155

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHTKSSFIKLALECQVLKFGEFTLKSGRISPYFFNAGLFYHGNSIRQLGQFYAKTLLEQ
CCCCHHHHHHHHHHHEEEECCCEEECCCCCCCEEEECEEEEECHHHHHHHHHHHHHHHHH
EISFEHLFGPAYKGIPLATATAVALAELGRDITVTFNRKEVKTHGEGGQLIGSPLTGRTV
HCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHEECCCCCCEECCCCCCCEE
IIDDVITAGTAFRESQTLIKENGGILRGVIIALDRCERGLTEKSTLSEIREQGIEVYSII
EEEHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHH
NLFDLIEFLKNDNQYEQVQKLESYHELYGAY
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNHTKSSFIKLALECQVLKFGEFTLKSGRISPYFFNAGLFYHGNSIRQLGQFYAKTLLEQ
CCCCHHHHHHHHHHHEEEECCCEEECCCCCCCEEEECEEEEECHHHHHHHHHHHHHHHHH
EISFEHLFGPAYKGIPLATATAVALAELGRDITVTFNRKEVKTHGEGGQLIGSPLTGRTV
HCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHEECCCCCCEECCCCCCCEE
IIDDVITAGTAFRESQTLIKENGGILRGVIIALDRCERGLTEKSTLSEIREQGIEVYSII
EEEHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHH
NLFDLIEFLKNDNQYEQVQKLESYHELYGAY
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA