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The map label for this gene is hisH [H]

Identifier: 54294140

GI number: 54294140

Start: 1346395

End: 1346994

Strand: Reverse

Name: hisH [H]

Synonym: lpl1204

Alternate gene names: 54294140

Gene position: 1346994-1346395 (Counterclockwise)

Preceding gene: 54294141

Following gene: 54294139

Centisome position: 40.26

GC content: 38.33

Gene sequence:

>600_bases
ATGATTGCTGTGATTGATGTCAGTGGGAATAATTTAACTTCTCTGACAAATGCCTTGATTCGATTGGGAGGGCATTTTGC
ACTGACCCATGATGCTCAAGAAATACAAAAAGCAAGCCATGTCATTTTGCCAGGGGTTGGAACAGCACGTTCTGGTATGA
CAGCTTTGCAGCAAAATGGGCTAATAGACGTTTTAAGAACCTTAACCCAACCACTTCTTGGCATCTGTCTTGGCATGCAA
TTATTGCTTGAATACAGTGAAGAAGACGACATACCCTGCCTGGGTTTGATTCCTGGAGTTGCAGAGTTATTAAAAGCTGA
AAGAAATCATCCTGTTCCTCATATGGGATGGAACAATCTTCATTGGCAAAAAACATCATCATTACAACAAGGATTAAATA
ATAGTGATTATGTTTATTTTGTTCATAGCTATGCTCTAAAAGCAGATGATTATGCATTGGCTCGATGTAGGTATCACGAA
GAGTTCACTGCAGTTGTAAAAAAAGGCAATTTTTATGGCATGCAATTTCACCCAGAAAAATCAGCCGATGTTGGCATGGT
ATTACTAAATAACTTTTTATCTTTGGAGTCTAAATGTTAG

Upstream 100 bases:

>100_bases
ATCACCACATGATCGAAGCATGCTTCAAAGTTTTAGGCAGAGCCTTGCGACAGGCATGCTCAAGAACGAATAATTATCTA
CCTTCAACCAAGGGTGTATT

Downstream 100 bases:

>100_bases
TTATTCCAGCTATTGATTTGCAATCTGGACGATGTGTGCGCTTAAAACAAGGACGGTTTGATCAAGTAACGCAATTTAGC
GTTTTTCCCATAGAAAGGGC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit 2; IGP synthase subunit hisH 2; ImGP synthase subunit hisH 2; IGPS subunit hisH 2 [H]

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MIAVIDVSGNNLTSLTNALIRLGGHFALTHDAQEIQKASHVILPGVGTARSGMTALQQNGLIDVLRTLTQPLLGICLGMQ
LLLEYSEEDDIPCLGLIPGVAELLKAERNHPVPHMGWNNLHWQKTSSLQQGLNNSDYVYFVHSYALKADDYALARCRYHE
EFTAVVKKGNFYGMQFHPEKSADVGMVLLNNFLSLESKC

Sequences:

>Translated_199_residues
MIAVIDVSGNNLTSLTNALIRLGGHFALTHDAQEIQKASHVILPGVGTARSGMTALQQNGLIDVLRTLTQPLLGICLGMQ
LLLEYSEEDDIPCLGLIPGVAELLKAERNHPVPHMGWNNLHWQKTSSLQQGLNNSDYVYFVHSYALKADDYALARCRYHE
EFTAVVKKGNFYGMQFHPEKSADVGMVLLNNFLSLESKC
>Mature_199_residues
MIAVIDVSGNNLTSLTNALIRLGGHFALTHDAQEIQKASHVILPGVGTARSGMTALQQNGLIDVLRTLTQPLLGICLGMQ
LLLEYSEEDDIPCLGLIPGVAELLKAERNHPVPHMGWNNLHWQKTSSLQQGLNNSDYVYFVHSYALKADDYALARCRYHE
EFTAVVKKGNFYGMQFHPEKSADVGMVLLNNFLSLESKC

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=195, Percent_Identity=42.0512820512821, Blast_Score=165, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6319725, Length=214, Percent_Identity=35.0467289719626, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 21993; Mature: 21993

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAVIDVSGNNLTSLTNALIRLGGHFALTHDAQEIQKASHVILPGVGTARSGMTALQQNG
CEEEEEECCCCHHHHHHHHHHHCCCEEEECCHHHHHHHCCEEECCCCCCHHHHHHHHHCC
LIDVLRTLTQPLLGICLGMQLLLEYSEEDDIPCLGLIPGVAELLKAERNHPVPHMGWNNL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCC
HWQKTSSLQQGLNNSDYVYFVHSYALKADDYALARCRYHEEFTAVVKKGNFYGMQFHPEK
CCCCHHHHHCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
SADVGMVLLNNFLSLESKC
CCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIAVIDVSGNNLTSLTNALIRLGGHFALTHDAQEIQKASHVILPGVGTARSGMTALQQNG
CEEEEEECCCCHHHHHHHHHHHCCCEEEECCHHHHHHHCCEEECCCCCCHHHHHHHHHCC
LIDVLRTLTQPLLGICLGMQLLLEYSEEDDIPCLGLIPGVAELLKAERNHPVPHMGWNNL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCC
HWQKTSSLQQGLNNSDYVYFVHSYALKADDYALARCRYHEEFTAVVKKGNFYGMQFHPEK
CCCCHHHHHCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
SADVGMVLLNNFLSLESKC
CCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA