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The map label for this gene is pyrB [H]

Identifier: 54293570

GI number: 54293570

Start: 674741

End: 675634

Strand: Direct

Name: pyrB [H]

Synonym: lpl0622

Alternate gene names: 54293570

Gene position: 674741-675634 (Clockwise)

Preceding gene: 54293569

Following gene: 54293574

Centisome position: 20.17

GC content: 38.37

Gene sequence:

>894_bases
ATGAAGCATTTTATTGAAATTAGTCAATTGTCATCTGAACAAATTGAATCCTTATTGCAACGAGCTTTATACTTTAAACA
CACAAAACAATACCCTTCTTATTCCCAAAGTATTATTGCTAATTTATTTTATGAAAACAGTACCAGAACCCGGATCAGTT
TTGAGTTAGCAGAGCGCCATCTGGCAATGTCTGTAGTGAATCTGGATCTTGAGACCTCATCAGAAACTAAAGGCGAAGCC
ATTGAAGACACCATCAGAACGCTAGCCGCTATGGGTATCCAGTATTTTGTAATTCGCCATAAGCAAGATGGGCTGCAGCA
AAATTTAGCTAATAAGTTAGGTGATACAGTTCATATAATAAATGCTGGTGATGGAACTCATGCACATCCAAGCCAGGCGA
TATTGGATATGGTGACAATTGTTGAACAAAAAAAGCAATTAGACAAATTAAAAATTGCTATTTTGGGAAATATCAAACAT
TCACGTGTTGCTAATTCTTTCCAATGCATTTGCAGTAAATTAGGGGTTGGTGAGTTGGTTTTGATTTCTCCGGAGATTTG
GCAACCATCACAAGTGCACTTTGGACGGGTTACTGATAATCTCAATGAAGGTCTGGAAGGTGCGGATGTAGTAATTTGCC
TGCGAGTTCAAAAGGAACGTTTGCTGCAAGATGATCATTTGGATTTGGATTTTTATAGAAACAATTTTGCACTTACTCAA
AAAAGTCTGTCTTATGCAAAACCTGACGCTATGGTTATGCACCCTGGGCCTATGAATCGCGGGGTTGAAATTGATAGTGA
AGTGGCTGATGGCAACCAATCTTGTATTTTACAACAGGTTACTAATGGTGTTTATGCAAGAATGGCTATCCTTGAATCGC
TGATAGCGAGTTAG

Upstream 100 bases:

>100_bases
AGTATAGCAGCTTGTGTTATACTGGAGCAGTGGTTGCAACAATCGGAATAACTCAAAGAGTATTTTTCTTTTGGCGTAAA
ATGAAATTTGGCGGGGGAAC

Downstream 100 bases:

>100_bases
ATTTCTCTATATTCTGTCATACATTGTGCTTTTATTCTGTTGGGTAATATATTCTACTCATACTGCAAGACGCATAATTA
ACCACGCTGAGCAAGAGATA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MKHFIEISQLSSEQIESLLQRALYFKHTKQYPSYSQSIIANLFYENSTRTRISFELAERHLAMSVVNLDLETSSETKGEA
IEDTIRTLAAMGIQYFVIRHKQDGLQQNLANKLGDTVHIINAGDGTHAHPSQAILDMVTIVEQKKQLDKLKIAILGNIKH
SRVANSFQCICSKLGVGELVLISPEIWQPSQVHFGRVTDNLNEGLEGADVVICLRVQKERLLQDDHLDLDFYRNNFALTQ
KSLSYAKPDAMVMHPGPMNRGVEIDSEVADGNQSCILQQVTNGVYARMAILESLIAS

Sequences:

>Translated_297_residues
MKHFIEISQLSSEQIESLLQRALYFKHTKQYPSYSQSIIANLFYENSTRTRISFELAERHLAMSVVNLDLETSSETKGEA
IEDTIRTLAAMGIQYFVIRHKQDGLQQNLANKLGDTVHIINAGDGTHAHPSQAILDMVTIVEQKKQLDKLKIAILGNIKH
SRVANSFQCICSKLGVGELVLISPEIWQPSQVHFGRVTDNLNEGLEGADVVICLRVQKERLLQDDHLDLDFYRNNFALTQ
KSLSYAKPDAMVMHPGPMNRGVEIDSEVADGNQSCILQQVTNGVYARMAILESLIAS
>Mature_297_residues
MKHFIEISQLSSEQIESLLQRALYFKHTKQYPSYSQSIIANLFYENSTRTRISFELAERHLAMSVVNLDLETSSETKGEA
IEDTIRTLAAMGIQYFVIRHKQDGLQQNLANKLGDTVHIINAGDGTHAHPSQAILDMVTIVEQKKQLDKLKIAILGNIKH
SRVANSFQCICSKLGVGELVLISPEIWQPSQVHFGRVTDNLNEGLEGADVVICLRVQKERLLQDDHLDLDFYRNNFALTQ
KSLSYAKPDAMVMHPGPMNRGVEIDSEVADGNQSCILQQVTNGVYARMAILESLIAS

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=307, Percent_Identity=30.6188925081433, Blast_Score=139, Evalue=3e-33,
Organism=Escherichia coli, GI2367364, Length=301, Percent_Identity=32.2259136212625, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1790703, Length=336, Percent_Identity=25, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786469, Length=337, Percent_Identity=25.8160237388724, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=326, Percent_Identity=31.2883435582822, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322331, Length=272, Percent_Identity=31.25, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6322373, Length=325, Percent_Identity=26.1538461538462, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24642586, Length=313, Percent_Identity=30.0319488817891, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 33278; Mature: 33278

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHFIEISQLSSEQIESLLQRALYFKHTKQYPSYSQSIIANLFYENSTRTRISFELAERH
CCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEHHHHHHH
LAMSVVNLDLETSSETKGEAIEDTIRTLAAMGIQYFVIRHKQDGLQQNLANKLGDTVHII
HHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHCCCEEEEE
NAGDGTHAHPSQAILDMVTIVEQKKQLDKLKIAILGNIKHSRVANSFQCICSKLGVGELV
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHCCCCEEE
LISPEIWQPSQVHFGRVTDNLNEGLEGADVVICLRVQKERLLQDDHLDLDFYRNNFALTQ
EECCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECHHHHCCCCCCCEEEECCCCHHHH
KSLSYAKPDAMVMHPGPMNRGVEIDSEVADGNQSCILQQVTNGVYARMAILESLIAS
HHHHHCCCCEEEECCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKHFIEISQLSSEQIESLLQRALYFKHTKQYPSYSQSIIANLFYENSTRTRISFELAERH
CCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEHHHHHHH
LAMSVVNLDLETSSETKGEAIEDTIRTLAAMGIQYFVIRHKQDGLQQNLANKLGDTVHII
HHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHCCCEEEEE
NAGDGTHAHPSQAILDMVTIVEQKKQLDKLKIAILGNIKHSRVANSFQCICSKLGVGELV
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHCCCCEEE
LISPEIWQPSQVHFGRVTDNLNEGLEGADVVICLRVQKERLLQDDHLDLDFYRNNFALTQ
EECCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECHHHHCCCCCCCEEEECCCCHHHH
KSLSYAKPDAMVMHPGPMNRGVEIDSEVADGNQSCILQQVTNGVYARMAILESLIAS
HHHHHCCCCEEEECCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA